Documente Academic
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Documente Cultură
1
Vol.
1
No.
1,
2012
Journal
of
Applied
Food
Technology
Lactose
and
Reduction
Sugar
Concentrations,
pH
and
the
Sourness
T(2%);
T3
(4%);
and
T4
(6%).
The
raw
was
incubated
in
effect
of
some
parameters.
The
result
of
measured
incubator
for
5
hours.
parameters
in
this
study
is
presented
in
Table
1.Tabel
1.
Lactose
Concentration,
Total
Reduction
Sugar,
pH
and
Level
Measurement
of
Lactose
Concentration
of
Sourness
of
Date
Flavored
Yogurt
Drink.
Different
was
used
Telles
Method.
Sample
was
diluted
up
to
superskrip
indicate
difference
result
based
on
statistic
200
dilution,
then
poured
2,5ml
of
simple
into
closed
test
analysis,(
ns
)
indicate
there
is
no
differences
(P<0,05).
tube
and
added
ZnSO4
5%
dan
Ba(OH)2
4,5%
each
reagent
As
seen
in
table
1,
adding
date
with
different
0,2ml.
Simple
was
centrifuged
on
speed
1000rpm
for
5
percentage
gave
variety
impact
to
all
parameters.
Lactose
minutes
till
appeared
the
sediment.
1
ml
of
supernatant
was
concentration
and
total
reduction
sugar
had
correlation
for
poured
into
closed
test
tube
and
added
Telles
Reagent,
then
assuming
the
utility
of
those
kinds
of
sugar.
The
data
gave
boiled
on
waterbath
for
6
minutes.
Aquades
was
added
into
detail
that
the
lower
lactose
concentration
means
the
higher
test
tube
until
the
volumen
became
12,5ml
and
shaked
it
lactose
was
used
by
LAB.
The
more
addition
of
date
into
well
(Teles
et
al.,
1978).
Read
the
sample
result
into
yogurt
drink
gave
impact
to
total
reduction
sugar
in
yogurt.
spectrometer
in
the
formula
bellow:
However
the
data
showed
that
the
more
total
reduction
X
(gram/100ml)
sugar,
the
lower
pH
value,
and
it
also
indicate
that
the
lower
Lactosa
concentration
=
--------------------------
pH
value,
the
higher
activities
of
LAB
in
producing
lactic
acid.
B
(gram)
Adding
different
percentage
of
date
into
yogurt
drink
influenced
the
ulitity
of
lactose
in
fermented
process
Information
:
(Fig.
1).
Lactose
concentration
of
yogurt
drink
based
on
this
X
:
Result
of
conversin
absorbant
sample
study
showed
tendency
to
decrease
from
T0
to
T2
but
still
in
B
:
weight
of
sample
same
range
of
statistic
analysis.
Treatment
T3
showed
the
highest
lactose
concentration.
The
study
informed
that
LAB
Measurement
of
pH
value
mostly
using
lactose
from
T0-T2,
and
in
T3
was
assumed
that
pH
value
was
used
pH
digital.
Sample
was
poured
LAB
used
another
kinds
of
sugar
to
grow.
Lactose
can
be
into
tube
and
then
put
indicator
of
pH
digital
on
sample.
degreed
by
LAB
and
used
the
carbon
as
energy
resource.
Read
the
result
on
pH
digital.
Mazahreh
dan
Ershidat
(2009),
state
that
Lactobacillus
Measurement
of
Reduction
Sugar
Concentration
bulgaricus
Streptococcus
thermophilus
able
to
degreed
sample
poured
into
flask
100ml
about
2ml
and
added
lactose
to
be
glucose
and
galactose
up
to
20-30%
of
total
aquadest
until
the
volume
100ml,
shaked
it
well
to
make
it
lactose
in
milk.
Yildiz
(2010)
also
clarify
that
Lactobacillus
homogen.
Put
10
ml
of
sampel
into
enlemeyer
then
poured
achidophilus
break
the
lactose
to
be
lactic
acid.
Lactose
25ml
of
Luff
Schoorl
reagent.
The
sample
was
boiled
on
concentration
was
also
depend
on
the
ability
of
LAB
to
use
waterbath
for
10
minutes,
later
on
added
15
ml
KI
20%
dan
and
degreed
lactose.
35
ml
H2SO4
carefully
through
enlemeyer-wall.
Titrated
the
Result
of
total
reduction
sugar
increase
persistently
sample
with
Na-thioslfat
0,1
N
till
become
yellow,
then
from
T0
to
T3
(Fig.
2).
Adding
date
extract
into
yogurt
drink
added
amylum
1%
continued
titrated
untill
blue
color
increase
total
reduction
sugar
of
yogurt.
Amount
of
disappeared.
Made
Blanko
titrated
with
sample
25ml
Luff
reduction
sugar
in
yogurt
can
support
the
growth
of
Lactic
Schoorl
reagent.
The
Reduction
Sugar
concentration
gain
by
Acid
Bacteria.
Gad
et
al.
(2010)
stated
that
sugar
of
date
used
the
formulas
bellow
:
palm
stimulate
the
lactic
acid
bacteria
activities.
El-
Sharnouby
et
al.
(2009)
said
that
date
contains
of
83.51%
Approach
of
result
x
P
Reduction
Sugar
Concentration
=
--------------------------------------
x
100%
reduction
sugar
such
as
glucose
and
fructose.
Reduction
Weight
of
Sampel
sugar
is
kind
of
sugar
which
encourage
LAB
to
grow.
Information
:
However
too
much
sugar
in
yogurt
will
not
good
for
LAB
to
Approach
of
Result
:
approach
of
table
Luff
Schrool
table
grow.
Lubis
(2011)
also
stated
that
adding
date
extract
into
P
:
dilution
yogurt
drink
increase
Total
LAB
significantly.
Yildiz
(2010)
state
that
range
fruit
addition
into
yogurt
drink
better
from
RESULT
AND
DISCUSSION
30-35%
containing
fructose
2.7%
in
maximum
and
sucrose
Based
on
this
research
make
clear
that
yogurt
drink
less
than
4%.
Amount
of
added
date
extract
into
yogurt
which
was
added
by
date
increased
total
lactic
acid
bacteria
drink
give
impact
to
total
reduction
sugar,
the
more
addition
and
it
influenced
some
others
parameter.
Different
of
date
extract
into
yogurt
drink,
the
more
total
reduction
percentage
date
added
into
yogurt
drink
made
different
sugar
in
yogurt
drink.
2
Vol.
1
No.
1,
2012
Journal
of
Applied
Food
Technology
Lactose
and
Reduction
Sugar
Concentrations,
pH
and
the
Sourness
In
Fig.
3
showed
that
pH
value
decreased
from
T0
to
El-Sharnouby,
G.
A.,
S.
M.
Al-Eid
and
M.
M.
Al
Otaibi.
2009.
T3.
The
more
addition
of
date
extracts
the
more
lower
pH
Utilization
of
enzymes
in
the
production
of
liquid
value
of
yogurt
drink.
pH
value
were
caused
by
activities
of
sugar
from
dates.
African
J.
of
Biochem.
3
(3)
:
041-
047.
LAB
in
forming
lactic
acid.
Yildiz
(2010)
said
that
Gad,
A.
S.,
A.
M.
Kholif
dan
A.
F.
Sayed.
2010.
Evaluation
of
Lactobacillus
achidophilus
is
able
to
produce
and
accumulate
the
nutritional
value
of
functional
yogurt
resulting
lactic
acid
so
that
decrease
the
pH
value.
Based
on
the
from
combination
of
date
palm
syrup
and
skim
research
of
Lubis
(2011),
adding
more
date
extract
milk.
Am.
J.
Food
Technology.
5
(5)
:
250-259.
accumulated
the
acidity
of
yogurt
drink.
Base
on
statistic
Marshall,
J.
2006.
Makanan
Sumber
Tenaga.
Erlangga,
analyzed
adding
date
into
yogurt
drink
gave
no
influence
to
Jakarta.
the
sourness
by
panelist
(Fig.
4).
So
the
different
percentage
Mazahreh,
A.
M
and
O.
T.
M.
Ershidat.
2009.
The
Benefits
of
lactic
acid
bacteria
in
yogurt
on
the
gastrointestinal
of
added
date
range
from
0%-6%
gave
no
impact
in
level
of
function
and
health.
Alia
University
College,
Jordan.
sourness.
8
(9)
:
1404-1410.
Legowo,
A.
M.,
Kusrahayu
dan
S.,
Mulyani.
2009.
Teknologi
CONCLUSION
dan
Teknologi
Susu.
Universitas
Diponegoro,
This
study
confirmed
that
adding
date
extract
into
Semarang.
yogurt
drink
added
up
the
amount
of
reduction
sugar
Lubis,
B.
2011.
Kadar
Air,
Keasaman,
Total
Bakteri
Asam
significantly
and
support
LAB
to
produce
lactic
acid.
The
Laktat,
Dan
Kesukaan
Compound
Yogurt
Drink
Berflavor
Kurma
(Phoenix
Dactylifera)
Sebagai
more
lactic
acid
of
yogurt,
the
lower
of
pH
value.
The
best
Minuman
Probiotik.
Program
Sarjana
Universitas
treatment
percentage
added
date
yogurt
drink
was
placed
in
Diponegoro,
Semarang.
(Skripsi
Sarjana
T2
adding
4%
of
date
extracts,
because
the
result
of
all
Peternakan).
parameter
mostly
not
give
significant
influence.
Tamime,
A.
2006.
Fermented
milk.
Blackwell
Science,
UK.
Teles,
F.
F.
F.,
Cheryl
K.Y.,
and
J.
W.
Stull.
1978.
A
Method
for
REFERENCES
Rapid
Determination
of
Laktose.
J.
Dairy
Sci
61:
Al-Khuzaim,
M.
2010.
Khasiat
Kurma
dan
Mukjizat
Kurma
506-508.
Ajwah.
Perpustakaan
Nasional
RI,
Solo.
Yildiz,
F.
2010.
Development
and
Manufacture
of
Yogurt
and
Diwangkoro,
G.
(2008).
Pengaruh
Lama
Inkubasi
pada
Proses
Other
Functional
Dairy
Products.
Taylor
and
Francis
Pembuatan
Yogurt
Susu
Kambing
yang
Group,
United
State.
Ditambahkan
Buah
Durian
5%
Terhadap
Total
Bakteri
Asam
Laktat,
Kadar
Laktosa
dan
nilai
pH.
Program
Sarjana
Universitas
Diponegoro,
Semarang.
(Skripsi
Sarjana
Peternakan).
3
Vol.
1
No.
1,
2012
Journal
of
Applied
Food
Technology