Biology 10

(Osmosis Lab Report)

By

Anupunswang Aticha (Bam)

Koosongdham Bhu (Foonjakrawan)

Ngarmpornchai Witchayut (Beam)

Sangwong Tanat (Tai)

Thoumprathom Masna (Na)

1002

Date: 6th March, 2017

Introduction
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Purpose

The purpose of the lab is to understand more about passive transport in osmosis
way. Osmosis is a movement of water from high concentration of water to low concentration
of water in the condition that only solvent can pass but solute can not.

Hypothesis

How will soaking the Jelly in distilled water affect the size of the candy?
Hypothesis: If we soak the jelly in the distilled water, then the jelly will increase in size.

How will soaking Jelly in concentrated salt water affect the size of the candy?
Hypothesis: If we soak the jelly in the concentrated salt water, then the jelly will decrease in
size.

How will soaking the Potato in distilled water affect the size?
Hypothesis: If we soak the potato in distilled water, then the potato will be the same size.

How will soaking the Potato in concentrated salt water affect the size?
Hypothesis: If we soak the potato in concentrated salt water, then the potato will decrease in
size.

Materials

1. 6 Beakers (3 for jelly, 3 for potato)

2. Electronic Balance
3. Distilled water
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4. Concentrated salt water

5. 3 Jellies
6. 3 Potatoes Pieces
7. Calculator a groups bring their own calculator
8. Knife
9. Weighing paper
10. Markers
11. Gloves
12. Lab Coat
13. Goggles

Methods
1. Collect the materials that your group needs (3 Jelly and Half a Potato)
2. Use the knife to cut the potato into 3 equal pieces
3. Use the electronic balance to find the mass of your jelly. Remember to lay
down a piece of weighing paper and set to zero.
4. Record your data
5. Record descriptive observations about the jelly prior to the experiment.
6. Measure the initial volume of the jelly and the potato and record your data.
7. Fill your 1st beaker halfway with distilled water. Put one of your jelly into the
beaker. Set the beaker aside for one hour. Label the beaker using marker.
8. Fill your 2st beaker with concentrated salt solution halfway. Put your 2nd jelly
into that beaker. Set beaker aside for one hour. Label the beaker using marker.
9. Put your 3rd jelly into an empty beaker without putting anything in it. That
should be your control.
10. Put the 4th beaker halfway with distilled water. Put one of your potato pieces
into the beaker. Set the beaker aside for one hour. Label the beaker.
11. Put the 5th beaker halfway with concentrated salt solution halfway. Put your
second potato pieces into that beaker and set it aside for an hour. Label the beaker.
12. Place your last piece of potato pieces into the last beaker, empty. That is your
control for the potato. Label the beaker.
13. Make sure you label all the beakers distilled water or concentrated salt
solution with some markers.
14. After one hour, come back and look at each of the beakers and record the
data volume and mass of both the jellies in two beaker and both the potato in two
other beakers.
15. Also record the descriptive data for what the jelly and the potato look like now
after being soaker for an hour.
16. Calculate the percent change in the size of each jelly and potato

Data Tables

A: The volume and mass of the two jellies before and after soak into hypertonic and
hypotonic solutions

Jelly 1 Jelly 2 (Color)

Dimensions Initial: Before Final: After Initial: Before Final: After

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soaking in soaking in soaking in salt soaking in salt

distilled water distilled water water water

Volume 3 mL 6 mL 4 mL 4.5 mL

Mass 5.1 g 6.42 g 5.10 g 5.06 g

B: The volume and mass of the two potatoes before and after soak into hypertonic and
hypotonic solutions

Potato 1 Potato 2 (Color)

Dimensions Initial: Before Final: After Initial: Before Final: After

soaking in soaking in soaking in salt soaking in salt
distilled water distilled water water water

Volume 4 mL 6 mL 5 mL 4.5 mL

Mass 5.27 g 5.87 g 5.22 g 4.33 g

C: The description of the two jellies before and after soak into hypertonic and hypotonic
solutions

Jelly 1 put into distilled water: (Color) Jelly 2 put into salt water: (Color)

Descriptive Observations Before Soaking in Descriptive Observations Before Soaking in

Distilled Water: Salt Water:
Round purple jelly that slighty sticks to the Round purple jelly that slighty sticks to the
surface it touches surface it touches

Descriptive Observations After Soaking in Descriptive Observations After Soaking in Salt

Distilled Water: Water:
It swells a little bit and becomes more slippery The size of the jelly is the same, but there was
with a swelled part that is half transparent. a white substance around the jelly which can
be easily broken down when we touch it.
D: The description of the two potatoes before and after soak into hypertonic and hypotonic
solutions

Potato 1put into distilled water: (Color) Potato 2 put into salt water: (Color)

Descriptive Observations Before Soaking in Descriptive Observations Before Soaking in

Distilled Water: Salt Water:
The potato is yellow, and it is a little bit The potato is yellow, and it is a little bit
moist. The potato is also not slippery moist. The potato is also not slippery

Descriptive Observations After Soaking in Descriptive Observations After Soaking in

Distilled Water: Salt Water:
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The potato size is a little bit bigger, and its It looks just slightly smaller and
edge becomes whiter. Also, the potato is
still not slippery.

E: The percent change in volume and mass of the two jellies after soak into hypertonic and
hypotonic solutions

Jelly 1 (Color): Soaked in Jelly 2 (Color): Soaked in

Distilled Water Salt Water

Percent Change in Volume 100% 12.5%

Percent Change in Mass 25.88% 0.78%

F: The percent change in volume and mass of the two potatoes after soak into hypertonic
and hypotonic solutions

Potato 1 (Color): Soaked in Potato 2 (Color): Soaked in

Distilled Water Salt Water

Percent Change in Volume 50% 10%

Percent Change in Mass 11.39% 17.05%

Bar Graph

The percentage change in mass and volume of jelly

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The percentage change in mass and volume of potato

Analysing Results

1. What happened to the jellies after being soaked in distilled water and salt water for an
hour? Why? Be descriptive and detailed in your answer. Use your knowledge of membrane
transport.
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Answer: The jelly that was put into the distilled water swelled a little bit from its original size.
The jellys mass increased from 5.10 to 6.42 gram and its volume from 3 to 6 mL due to the
osmosis reaction. Since the jelly has more solute concentration than the solution which was
the distilled water according to the osmosis reaction, water will move from lower
concentration of solute to the higher concentration of solute; therefore water moved from the
solution into the jelly. So the jelly swelled a little and increased in mass and volume.
According to the osmosis reaction, if we put the jelly which may have less solute
concentration than the salt water solution, then the jelly would shrink and decrease in mass
and volume. However, our result from putting jelly into the salt water was that its mass
decreases, and its volume increases. The jelly mass changed from 5.10 to 5.06 gram and
volume changed from 4 to 4.5 mL. From this result, we assume that we havent put the jelly
into the solution long enough to get the accurate result which the jellys mass and volume
both decreases

2. What happened to the potato after being soaked in distilled water and salt water for an
hour? Be descriptive and detailed in your answer. Use your knowledge of membrane
transport?

Answer: The potato that was put into the salt water decreased in size, volume and mass
comparing with the original size. The mass of the potato is decreased about 0.89 grams. The
volume is decreased 0.5 mL. It is decreased because the potato has less concentration than
the solution. Since in the osmosis reaction, the water will move from less concentration of
solute to the higher concentration of solute; therefore water will move out from potato to salt
water.
On the other hand, the potato that was put into the distilled water is increased in size
and both volume and mass. The mass is increased about 0.60 grams, and the volume is
increased 2 mL. According to the osmosis reaction, It is increased because the water will
move to the higher concentration of solute. So the water will move into the potato.

Conclusion

In conclusion, water mostly goes outwards the jelly and potato in the hypertonic
solution; hence, their mass and volume are decreased. On the other hand, water goes
inwards the jelly and potato in the hypotonic solution; hence, their mass and volume are
increased. The osmosis reaction occurs in these jellies and potatoes mass and makes their
volumes change.
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Work Log

Name: Job / Responsible:

Bhu K. Data table, analyse 1

Na T. Data table, Bar graph

Tai S. Data table, analyse 2, adjust layout

Beam N. Purpose, Data table, Conclusion

Bam A. Data table, material, method, analyse 1