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Department of Pathology, The First Affiliated Hospital of Sun Yat-sen University 58, Zhongshan
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Abstract: Nasopharyngeal carcinoma (NPC) is a tumor arising from the epithelial cells that
cover the surface and line the nasopharynx. Regardless of geographic distribution, racial
background and enigmatic etiology of NPC, A major problem has been the lack of a universally
accepted system for histopathologic classification of NPC. To alleviate this problem, the World
keratinizing squamous cell carcinoma and nonkeratinizing carcinoma since 1978, although this
classification system has not been accepted worldwide. During the past 50 years, several NPC
histological classifications have been published and adopted in clinic. As a high-incidence area
of NPC, until new classification published in 2001, Chinese NPC classifications can not be very
well to link with the international classifications of NPC, which makes great misunderstandings
in international communication. Actually there is still a long way to go to reach the consensus on
histological classification of NPC. This review will retrospectively link the difference of these
systems.
Introduction
Regardless of geographic distribution, racial background and enigmatic etiology of NPC, a major
problem has been the lack of a universally accepted system for histopathologic classification of
NPC. Although World Health Organization (WHO) advocates its diagnostic designation, there
have several histological classification systems been used to identify the variants of NPC in high-
and low-incidence regions. According to the WHO classification, NPC encompasses squamous
cell carcinoma, non-keratinizing carcinoma and basaloid squamous cell carcinoma. The non-
keratinizing type is further subdivided into differentiated and undifferentiated [1]. Therefore, this
strict definition of NPC by WHO is to the exclusion of all other malignant tumors that may arise
and lymphomas. However, before the criteria of WHO classification system is accepted in the
worldwide, there is still a long way to go to reach the consensus on histological classification of
NPC. In fact, Chinese classification system of NPC has not completely unified to WHO system.
The nasopharynx is the narrow tubular passage behind the nasal cavity. Its sloping roof and
posterior wall are formed by the basi-sphenoid, basi-occiput and the first cervical vertebra.
Anteriorly, it communicates with the nasal cavity via the choanae. The orifices of Eustachian
tubes are in the lateral walls, and each is shielded superiorly and posteriorly by a comma-shaped
elevation called the torus tubarius. Immediately above and behind the torus tubarius is a
pharyngeal recess called the fossa of Rosenmuller. The nasopharynx tapers inferiorly, and
continues as the oropharynx from the level of the soft palate. The nasopharynx constitutes part of
the Waldeyer ring. Histologically, its mucosa is covered by respiratory-type ciliated epithelium,
but variable amounts of squamous epithelium are common. The mucosa exhibits invaginations,
forming crypts that abut the underlying stroma. The stroma is rich in lymphoid tissue that often
includes reactive lymphoid follicles. The surface or crypt epithelium is commonly infiltrated by
many small lymphoid cells, which expand and disrupt the epithelium to produce a reticulated
pattern. Some seromucinous glands are present, but they are not as abundant as in the nasal
mucosa. The most frequent site of origin for NPC is the lateral wall of the nasopharynx, an area
epithelium via an intervening intermediate epithelium which has ultrastructural properties similar
The histogenesis NPC has been a complex problem. The ultrastructural studies of Svoboda et al
in 1965 proposed a squamous cell origin for NPC based on the ultrastructural demonstration of
desmosomes and keratin fibrils (tonofilaments) [4]. The subsequent studies have supported this
concept and have postulated to classify all NPCs as variants of squamous cell carcinoma [5-6].
However, some studies, including ultrastructural data from Chinese NPC patients, have
suggested the possibility of a respiratory epithelial origin for NPC based on ultrastructural
studies showing common cellular organelles in normal respiratory epithelium and some NPC
tumor cells [7-10]. Lin and his colleagues showed that some NPC cells contained a cytoplasmic
vacuolar system with a well-developed Golgi complex and endoplasmic reticulum as well as
cytoplasmic tonofilaments. They proposed that NPC, at least some variant of NPC, might arise
from basal cells of respiratory epithelium [11]. Sichuan Medical College in China have
recognized 6 morphologic types of NPC cells based on ultrastructural findings: Type 1 and 2
contain abundant tonofilaments and desmosomes; Type 4 and 5 contain secretory vacuoles and
rough endoplasmic reticulum; Type 3 contains mixtures of types 1, 2, 4, and 5; however, Type 6
are poorly differentiated with very few organelles. According to the ultrastructural variability of
NPC cells, they suggest that perhaps at least some cases of NPC are in a state of differentiation
toward respiratory epithelium because there are some similarities between NPC cells and
respiratory epithelial cells [8]. Prasad suggests that NPC cells may be derived from epithelial
cells that have undergone metaplastic transformation from columnar (i.e., respiratory) to
squamous cells because his ultrastructural study reveals secretory granules, well-developed
Golgi complex, and granular endoplasmic reticulum appear to have intermingle with
tonofilaments and desmosomes in cytoplasm of NPC cells [12]. Batsakis and his colleagues
propose that undifferentiated or nonkeratinizing NPC arises from primitive stem cells or
indeterminate cells, or from intermediate epithelium, all of these cell types being capable of
differentiating into mature squamous or respiratory epithelium [13]. Shi et al. found that some
cases of NPC showed carcinoma in situ involving a respiratory epithelial mucosal surface, and
Their immunohistochemical study on NPC suggests that perhaps some non-keratinizing and
undifferentiated cases of NPC have a respiratory epithelial origin, although some squamous
morphologic ultrastructural features may be present [14]. At present, the histogenesis of NPC
mucosa origin has been proposed [1]. However, some researches on precursor lesions of NPC,
including dysplasia and carcinoma in-situ of nasopharyngeal mucosa, suggest that most NPCs do
not originate from NPC in-situ because an in-situ or intraepithelial component is rarely detected
in NPC [15-17]. Presumably, NPC derives from precursor cells in basal layers of the stratified
squamous epithelium, a finding further supported by the strong immunoreactivity for p63 in both
the tumor and normal basal cells. These progenitor cells appear to be multipotent and capable to
NPC was firstly described as skull base cancer in 1845 by Michaux (another argument was in
1837 by Derand-Fardel) [18-19]. In 1921, NPC was firstly classified as a separate entity and
tumor might originate from lymphoepithelium. Quick and Culter in 1927 put forward the name
of transitional cell carcinoma. In 1938, Cappell divided NPC into Schimincke type
lymphoepithelioma and Regaud type lymphoepithelioma. In 1967, the NPC symposium of UICC
(Union for International Cancer Control) classified NPC into 7 subtypes: classic epidermoid
carcinoma, clear cell carcinoma, spindle cell carcinoma, transitional cell carcinoma,
Shanmugaratnam divided NPC histopathology into two major types: squamous cell carcinoma
and undifferentiated carcinoma. Squamous cell carcinoma subdivided into classic carcinoma,
clear cell carcinoma and spindle cell carcinoma; undifferentiated carcinoma included vesicular
nucleus cell carcinoma, fused type and mixed type [22]. In 1978, the first edition of WHO
classification of tumors published its NPC histological classification, the tumor was divided into
three major types: type I (squamous cell carcinoma), type II (non-keratinizing carcinoma) and
type III (undifferentiated carcinoma) [23]. However, in 1982, WHO regional offices for the
western pacific suggested that WHO type II and type III NPC should be combined, and only
type I and type III NPC retained. In addition, WHO define the abbreviation of NPC was only
applicable for the nasopharyngeal cancer with squamous differentiation under the light
microscope [24]. It means that NPC did not include the cancers occurring in the nasopharynx
sarcomas and lymphomas. In 1991, the second edition of WHO classification of tumors revised
its NPC histological types. There were two main groups according to whether or not there is
clear evidence of squamous differentiation by light microscopy. One was squamous cell
carcinoma (keratinizing squamous cell carcinoma), the other was non-keratinizing carcinoma.
The latter was subdivided into differentiated and undifferentiated variants [25]. In this
classification, the keratinizing squamous cell carcinoma of type I in 1978 WHO classification
was retained as squamous cell carcinoma, while part of type I (non-keratinizing squamous cell
carcinoma), type II and III variants of 1978 WHO classification were included as non-
keratinizing carcinoma. Based on the second edition of WHO classification of NPC, in 2005,
WHO updated its NPC histological classification and basaloid squamous cell carcinoma was
Table 1. The main NPC histological classifications of Chinese and international system.
Liangs classification (1961) [43] Norms of China for NPC (1991) [45] 2001 classification (2001) [46] WHO classification
Moderately-differentiated
2. Poorly-differentiated type Differentiated type Differentia
Poorly-differentiated
Squamous cell carcinoma grade Vesicular nucleus cell carcinoma Mixed types
III
3. Adenocarcinoma
Salivary-gland type
microinvasive carcinoma
This variant of NPC is an invasive carcinoma showing obvious squamous differentiation at the
light microscopic level, in the form of intercellular bridges and /or keratinisation over most of the
tumor, morphologically similar to keratinizing squamous cell carcinoma occurring in other head
and neck mucosal sites. The degree of differentiation can be further graded as: well
differentiated, moderately differentiated and poorly differentiated. The tumor cell borders are
distinct and separated by intercellular bridges. The nuclei often show hyperchromasia, but not
vesicular pattern, and the degree of nuclear pleomorphism ranges from mild to marked. The
cells, neutrophils and eosinophils. Compared with non-keratinizing carcinoma, this variant of
NPC shows a greater propensity for locally advanced tumor growth and lower propensity for
lymph node metastasis. Some studies suggest that this subtype of NPC has lower responsiveness
to radiation therapy and a worse prognosis [26-28]. However, other studies have not found this
The frequency of keratinizing squamous cell carcinoma of NPC is varied. In high incidence
areas, such as southern China, only 1%-2% of NPC patients are this tumor histology, while the
corresponding histological distribution in low incidence areas (Japan and North America) is
13%-25% [1, 31-32]. However, Singapore shows this histological type in 17% of NPC patients,
although it is also in high incidence area [28]. Unlike non-keratinizing carcinoma, keratinizing
squamous cell carcinoma of NPC tend to carry lower copy numbers of EBV, and nuclear signals
of EBER are usually confines to the less differentiated cells (basal cells that surround the
individual tumour islands), but not in the cells showing obvious squamous differentiation on in
Non-keratinizing carcinoma
Non-keratinizing carcinoma of NPC comprises over 95% of NPC in high incidence areas and
approximately 75%-87% of NPC in low incidence areas [1, 31-32]. These tumors are generally
more radiosensitive than squamous cell carcinoma and have stronger relationships with EBV
[34-36]. The tumors are often infiltrated by abundant lymphocytes. This group comprises a
and different areas of the same tumor or different biopsies taken at different time intervals from
the same patient may exhibit features of one or the other subtype. When both subtypes are seen
in a specimen, the tumor may be classified according to the prominent subtype, or as non-
indistinct cell borders, round to oval vesicular nuclei, and large central nucleoli. The cells often
appear crowded or even overlapping. The scant cytoplasm is either amphophilic or eosinophilic.
Abundant lymphocytes and plasma cells infiltrate the tumor islands, breaking them up into tiny
clusters or single cells and obscuring the epithelial nature of the tumor; the term
lymphoepithelial carcinoma may be applied for such cases. There can be small foci of
primitive squamous differentiation in this subtype. The undifferentiated type of NPC represents
over 90% of all NPCs in high incidence areas, and is the most frequent tumor type seen in
stratification and pavementing, often with a plexiform growth, reminiscent of transitional cell
carcinoma of the bladder. The tumor cells show fairly well-defined cell borders and sometimes
vague intercellular bridges, and there may exceptionally be occasional keratinized cells.
Compared with the undifferentiated subtype, the cells are often slightly smaller, the nuclear-
cytoplasmic ratio is lower, the nuclei can be more chromatin-rich, and nucleoli are usually not as
prominent. This type of NPC is uncommon, representing approximately 7%-12% of all NPCs in
southern China [1]. However, it is almost equal to undifferentiated type in Singapore with
Only a few cases of primary basaloid squamous cell carcinoma of NPC have been described in
literature [38-41] and represent less than 0.2% of all NPCs in southern China [1]. The
morphological features of this type are identical to the same tumor more commonly occurring in
other head and neck sites. Basaloid cells are small, with hyperchromatic nuclei without nucleoli,
and scant cytoplasm. They are closely packed, growing in a solid pattern with a lobular
configuration, and in some cases, there is prominent peripheral palisading. Comedo-type necrosis
and distinctive small cystic spaces containing PAS- and Alcian blue positive material can be
found in this type of NPC. The tumor appears to show a lower clinical aggressiveness compared
with basaloid squamous cell carcinoma occurring in other head and neck sites. The tumor cells of
this type in all Asian reported cases were positive to EBV, while only one Caucasian case was
In 1940s, lymphoepithelial carcinoma was used to describe this malignant tumor in China.
Since 1950s, some Chinese pathological experts have published a number of research papers
College divided NPC into 4 types: squamous cell carcinoma, transitional cell carcinoma,
classification of NPC proposed three main groups according to their histological characteristics
undifferentiated types. Well-differentiated type included squamous cell carcinoma grade I and II,
basal cell carcinoma and columnar cell carcinoma (adenocarcinoma). Poorly-differentiated type
included large round cell carcinoma, spindle cell carcinoma and squamous cell carcinoma grade
III. Undifferentiated type was pleomorphic cell carcinoma [43]. This histological classification
had a profound impact on subsequent classification of NPC in China. In 1979, the 5th Meeting of
Chinese NPC Prevention Collaboration held in Changsha city, China. Two classification strategy
of NPC were proposed in this meeting. Strategy I divided NPC into two groups: carcinoma in
situ and invasive carcinoma. Invasive carcinoma subdivided into 4 subtypes, which were
vesicular nucleus cell carcinoma (also known as large round cell carcinoma or lymphoepithelial
carcinoma), squamous cell carcinoma (the degree of differentiation was further graded as well-
differentiated and poorly differentiated), undifferentiated carcinoma and other rare carcinoma
such as adenoid cystic carcinoma, basal cell carcinoma and mucoepidermoid carcinoma. Strategy
II had also two main groups as strategy I: carcinoma in situ and invasive carcinoma. However,
[44].
In 1991, the Norms of China Common Cancer Clinical Diagnosis and Treatment was published,
and NPC was formally divided into carcinoma in situ and invasive carcinoma as two major
groups. Invasive carcinoma was sub-divided into micro-invasive carcinoma, squamous cell
differentiated and poorly-differentiated subtypes [45]. Like previously proposed Chinese NPC
NPC, which was not consistent with NPC histological classification recommended by WHO. In
addition, vesicular nucleus cell carcinoma was also identified as an independent entity of NPC
rather than a histological pattern of undifferentiated carcinoma. This classification has been
advocated widely and still being in use in China. However, there are great differences between
are frequently because of different NPC classification system. Therefore, in 2001, Zong and his
in line with international standard [46]. In the new 2001 Chinese classification of NPC, four
main groups were categorized, which were keratinizing squamous cell carcinoma, non-
differentiated types. Anaplastic squamous cell carcinoma, clear-cell squamous cell carcinoma,
pseudoglandular squamous cell carcinoma, papillary squamous cell carcinoma and basaloid
squamous cell carcinoma could be regarded as the specific histological patterns of keratinizing
squamous cell carcinoma with various degree of differentiation. Non-keratinizing carcinoma was
subdivided into differentiated, undifferentiated and mixed types. The histological diagnostic
criteria of these subtypes are similar to those in 1991 WHO classification of NPC. Mixed type of
non-keratinizing carcinoma was not included in WHO classification. Zong et al. identified this
type when the tumor exhibit features of both differentiated and undifferentiated type. However,
the authors considered that the mixed type of NPC might be combined with undifferentiated type
papillary adenocarcinoma, intestinal-type adenocarcinoma and signet ring cell carcinoma were
and mucoepidermoid carcinoma were the most common tumors of salivary-gland type
Whether or not carcinoma in situ and microinvasive carcinoma should be identified as distinct
carcinoma in situ is characterized by atypical epithelial change confined to the surface or crypt
epithelium, and lacking an invasive component. The epithelium is usually slightly thickened, and
consists of cells with variable loss of polarity, nuclear enlargement, nuclear crowding and distinct
nucleoli. 2001 Chinese classification further subdivided nasopharyngeal carcinoma in situ into
squamous cell type and columnar cell type according to the different type of epithelium
undergoing carcinogenesis [46]. Squamous cell carcinoma in situ was often observed at the
lining epithelium of nasopharynx, while columnar cell carcinoma in situ usually located at the
identified in only 3-8% of NPC cases, and pure nasopharyngeal carcinoma in situ, as confirmed
by multiple biopsies to rule out an invasive component, is very rare. Therefore, when
determine whether the invasive carcinoma has already existed at the other sites of
nasopharyngeal tissues. So far, all cases of nasopharyngeal carcinoma in situ studied have been
positive for EBV (EBER), confirming that EBV infection precedes the acquisition of
invasiveness by nasopharyngeal carcinoma. EBER may aid in the distinction between carcinoma
in situ and non-specific reactive atypia of the nasopharyngeal epithelium. Therefore, a few
studies suggest that pre-invasive NPC should be regarded as a rare but distinct entity [47-48],
and indeed a proportion of patients develop invasive cancer on follow-up [49]. At present, WHO
NPC classification has accepted the carcinoma in situ as a precursor lesion, but not a variant of
NPC [1]. Since nasopharyngeal carcinoma in situ has reliably identified histological appearances
and relevance for clinical outcome, we consider it should be classified as a distinct variant of
NPC, but not as a precursor lesion only, although it is indeed rare to be observed in clinical
practice.
The nasopharyngeal microinvasive carcinoma is designed when the tumor cells break the
basement membrane and infiltrate into the stroma, the extension of invasive cells is no more than
one high power field (under the objective 40 times) in 1991 and 2001 Chinese NPC classification
same patient. In fact, WHO has not described this lesion in NPC classification, and to our best
knowledge, so far microinvasive nasopharyngeal carcinoma has only described and discussed in
Chinese literature [46, 50]. Like nasopharyngeal carcinoma in situ, microinvasive carcinoma
microinvasive carcinoma is frequently associated with carcinoma in situ [46]. However, when
microinvasive carcinomas have been rarely encountered in the absence of an adjacent in situ
component, the diagnosis becomes challenging and puzzled. In addition, we found that there
were one or more separated microscopic foci of infiltration of tumor cells into the
whether or not the extension of infiltrating tumor cells is beyond the specialized fields under the
microscopy. Therefore, we suggest that the criteria for microinvasive carcinoma may be defined
when the greatest dimension in any one invasive focus is less than or equal to 1mm in size. Since
only a few of studies on nasopharyngeal microinvasive carcinoma are investigated, reliable data
on the clinical behavior of microinvasive carcinoma are not available and no predictive factors
have been identified. At present, microinvasive carcinoma can not be regarded as a distinct
variant of NPC. However, this lesion should be added into the NPC classification if the
prognoses of patients with microinvasive carcinoma have been convinced to be more excellent
than that in patients with invasive carcinoma. Of course, it is necessary that the strict definition
of microinvasive carcinoma is applied for diagnosis and this lesion can be clearly recognized
firstly.
Whether or not basaloid squamous cell carcinoma is a distinct variant of NPC or a histological
In 1961 Liangs classification, 1991 and 2001 Chinese classification, basaloid squamous cell
considered identifiable histological appearances, but that do not have a distinct clinical or
pathological significance. However, since 2005, basaloid squamous cell carcinoma has been
morphology, age distribution and biologic behaviour. The tumour appears to show a lower
clinical aggressiveness compared with basaloid squamous cell carcinoma occurring in other head
and neck sites. Basaloid squamous cell carcinoma has been accepted as a variant of squamous
cell carcinoma in other head and neck sites. But it is seldom paid attention in nasopharynx
because nasopharyngeal basaloid squamous cell carcinoma is rare, to our knowledge, so far only
6 such cases have been described in literature [38-41]. In fact, the association between this type
of tumor and its clinical behaviours has not been established yet. Mller and his colleagues have
follow: (1). younger median age at diagnosis as for other nasopharyngeal carcinomas; (2). very
slow development before diagnosis; (3). other etiological factors than nicotine and alcohol abuse
may play a role in oncogenesis; (4). presence of Epstein-Barr virus seems not to be correlated
with the basaloid squamous cell carcinoma of the nasopharynx; (5). possibly lower
aggressiveness than the tumor in other sites; (6). radical surgical and radiation therapy may
control the tumour [39]. Therefore, there are some differences between basaloid squamous cell
differences, we consider that particular attention must be paid to find the correlation between this
variant and its clinical observation, including prognosis and optimum treatment strategy. Once
nasopharyngeal basaloid squamous cell carcinoma has been convinced to have identifiable
clinical and pathological significance, it will be accepted as a distinct variant of NPC by Chinese
adenocarcinoma (NAC) as a clearly distinct variant of NPC, which might originates from the
lining epithelium or crypt, as well as small salivary glands of nasopharynx [43-46]. In 2001
Chinese classification, NAC was first classified into traditional (conventional) adenocarcinomas
and salivary gland-type adenocarcinomas. The traditional type was further subdivided into acinar
malignant mixed tumor. However, adenocarcinomas have been excluded by 1991 and 2005
carcinoma are low-grade carcinomas of nasopharynx with distinctive characteristics from NPC
NPC. (i) NPC has a unique geographical distribution, particularly in the Cantonese population of
China. In endemic areas, the undifferentiated non-keratinizing carcinoma accounts for more than
95%. In low incidence areas, keratinizing differentiated squamous cell carcinoma and
undifferentiated non-keratinizing carcinoma accounts for no more than 65%. However, the
geographical distribution of NAC is not distinct. The recent studies reveal that the incidence of
NAC of all cancers of nasopharynx is 0.11% in southern china, 0.38% in Hong Kong, 1.3% in
Taiwan, 0.7% in Italy, 0.0% in Uganda and 3.9% in USA [51-55]. These data suggest that the
incidence of NAC is extremely rare even among defined ethnic groups, and there is no specific
geographical distribution [56-57]. (ii) Epstein-Barr virus (EBV) may not be closely associated
with the carcinogenesis of NAC. A unique feature of NPC is its strong association with EBV.
Higher EBV antibody titers, especially of IgA class, are observed in most NPC patients. Latent
EBV infection is identified in cancer cells of virtually all cases of NPC in endemic regions.
Almost all of NPC cases have EBER positive by in situ hybridization (ISH). However, the
positive rate for the EBV antibody VCA-IgA in all types of NAC was 53% and only 24% in
the salivary gland type of NPAC [58]. More than 95% of conventional adenocarcinomas show no
EBV infection by EBER detection [46]. But some research data showed EBV was detected by
EBER ISH in 60% cases of salivary gland adenocarcinoma [52]. The prevalence of EA-IgA,
VCA-IgA and EBV DNase antibody plasma level were 13.3%, 25% and 42.9%, respectively in
adenoid cystic adenocarcinoma of the nasopharynx [59]. Due to its rarity, the relationship
between NAC and EBV is still unclear. (iii) Difference in clinical manifestation and prognosis.
The male to female ratio for NAC was reported to be 1.18:1, while the male to female ratio was
reported to be 2-3:1 in NPC. The majority of NPCs arise in the lateral walls, metastasis is
frequently found, and cervical lymphadenopathy is often the only clinical manifestation of NPC
patients. However, the slow rate of growth of NAC might be explained by the long duration of
symptoms before patients sought medical attention. Neck masses are uncommon in NAC due to
its low rate of cervical node metastasis. Due to the rarity of NAC, there is still no standard
postoperative treatment for patients who are at high risk of postoperative recurrence. But in high-
incidence endemic regions, the treatment of most NAC is similar to that of NPC. It is found that
NAC can be well controlled by radiotherapy with and without chemotherapy. Some research data
reveal that polymorphic low-grade adenocarcinomas, hyalinizing clear cell carcinomas and
acinic cell carcinomas in NAC has good prognosis [60]. Since NPC is highly radiosensitive,
radiotherapy has always been the main treatment of choice for this cancer. With conventional
practice and two-dimensional planning techniques, the local control rates were in the order of
80%, taking all T stages together. Intensity-modulated radiotherapy (IMRT) provides superior
local control and better delineation of tumor target volume [61]. Although overall survival after
radiotherapy for early staging is encouraging, there are significant rates of local failure and
Compared with NPC, NAC is very rare but distinct entity with various pathological types,
clinical manifestation and biological behaviours. We consider that NAC should not be regarded
as a variant of NPC. A slowly rate of growth, a tendency for cranial nerve invasion, a high
recurrence rate and a low distant metastatic rate are characteristic of NAC. Therefore, the clinical
tumor, node and metastases (TNM) staging system for NAC and optimal treatment strategies of
In 1991, Chinese NPC histological classification was published by the Norms of China Common
Cancer Clinical Diagnosis and Treatment. The invasive carcinoma was divided into micro-
invasive carcinoma, squamous cell carcinoma, adenocarcinoma, vesicular nucleus cell carcinoma
and undifferentiated carcinoma. Squamous cell carcinoma and adenocarcinoma included well-
same year, WHO classification divided NPC into keratinizing squamous cell carcinoma and non-
keratinizing carcinoma, the latter was sub-divided into differentiated type and undifferentiated
type. There are some questions when linking these two classifications of NPC. Which
undifferentiated type of WHO classification was puzzled. It was also confusion whether or not
undifferentiated carcinoma could be divided into four subtypes according to its morphological
and squamous cell carcinoma respectively [62]. Zong et al. revised their classification in 2001
and also considered the vesicular nucleus cell carcinoma or large round cell carcinoma was
1991 WHO classification should included undifferentiated carcinoma, large round cell
keratinizing carcinoma would exceed 90%, and the proportion of undifferentiated carcinoma
would be greatly reduced, if all poorly-differentiated squamous cell carcinoma in 1991 China
classification were included in the differentiated non-keratinizing carcinoma of 1991 WHO
classification, which was obviously inconsistent with the geographical distribution. However, it
Conclusion
Review the histological classification of NPC is important for pathologists to understand the
development history of NPC histological research. During the past 50 years, several NPC
histological classifications have been published and adopted in clinic. However, a major problem
at present is still a lack of a universally accepted system for using worldwide. As a high-
incidence area of NPC, until 2001 Chinese classification published, Chinese NPC classifications
can not be very well to link with the international classifications of NPC, which makes great
histological criteria to date for separating the different variants of NPC is based on cellular
morphology and growth patterns. Possibly this separation are not clinically meaningful. To date,
TNM staging system has been proven to be useful for predicting the prognosis of NPC patients.
However, the values of histological classification of NPC for predicting prognosis and
monitoring disease progression, including WHO and Chinese classification, are rather limited. In
most NPC studies, there are no correlation between histological classification and the patients
outcomes. Therefore, the future widely accepted NPC classification should meet the