Description

Designation:FDAstrainSeattle1946[DSM1103,NCIB12210]
DepositedName:Escherichiacoli(Migula)CastellaniandChalmers
ProductSheet AntigenicProperties:SerotypeO6,Biotype1
ProductDescription:Doesnotproduceverotoxin.ThisorganismisaCLSIcontrolstrainforantimicrobial
Escherichiacoli(ATCC susceptibilitytesting.Itisusedformediatesting,asanegativecontrolforLTtoxinproduction,andasaquality
controlstrainforAbbott,API,Autobac,BBL,bioMerieuxVitek,Biosynth,Difco,IDS,MicroMedia,MicroScan,
25922) RocheDiagnostics,andSensititreproducts.Usedinsusceptibilitydisctestingofneomycin,colistin[colimycin],
kanamycin,cephalexin,gentamicins,cefamandole,cephalothin,tetracycline,cephaloglycin,cephaloridine
[cephalomycin],nalidixicacid,andchloramphenicol.
PleasereadthisFIRST
Propagation
StorageTemp.
Frozen:80Cor Medium
colder ATCCMedium18:TrypticaseSoyAgar/Broth

FreezeDried:2C GrowthConditions
to8C Temperature:37C
LiveCulture:See Atmosphere:Aerobic
Propagation
PropagationProcedure
Section 1. Openvialaccordingtoenclosedinstructions.
2. Usingasingletubeof#18broth(5to6mL),withdrawapproximately0.5to1.0mLwithaPasteuror
1.0mLpipette.Rehydratetheentirepellet.
3. Asepticallytransferthisaliquotbackintothebrothtube.Mixwell.

BiosafetyLevel
4. Useseveraldropsofthesuspensiontoinoculatea#18agarslantand/orplate.
1
5. Incubatethetubesandplateat37Cfor24hours.

IntendedUse Notes

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.
CitationofStrain
Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Escherichiacoli(ATCC25922)
ATCC25922isarecommendedreferencestrainforantibioticsusceptibilitytesting.Ithasbeenfoundthat
passageinbrothoftenresultsinachangeinMIClevels.Therefore,itisbesttokeepitonagarandtomake
stocksforstorageimmediately.Repeatedpassageisdiscouraged.
Colonieson#18agarareentire,glistening,circular,smooth,translucent,andlowconvex.
PurifiedgenomicDNAofthisstrainisavailableasATCC25922D5.
AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.

References

Referencesandotherinformationrelatingtothisproductareavailableonlineatwww.atcc.org.

BiosafetyLevel:1

Appropriatesafetyproceduresshouldalwaysbeusedwiththismaterial.Laboratorysafetyisdiscussedin
thecurrentpublicationoftheBiosafetyinMicrobiologicalandBiomedicalLaboratoriesfromtheU.S.
DepartmentofHealthandHumanServicesCentersforDiseaseControlandPreventionandNationalInstitutes
forHealth.

ATCCWarranty

ATCCproductsarewarrantedfor30daysfromthedateofshipment,andthiswarrantyisvalidonlyifthe
productisstoredandhandledaccordingtotheinformationincludedonthisproductinformationsheet.Ifthe
AmericanTypeCultureCollection
POBox1549 ATCCproductisalivingcellormicroorganism,ATCCliststhemediaformulationthathasbeenfoundtobe
Manassas,VA20108USA effectiveforthisproduct.Whileother,unspecifiedmediamayalsoproducesatisfactoryresults,achangein
www.atcc.org mediaortheabsenceofanadditivefromtheATCCrecommendedmediamayaffectrecovery,growthand/or
functionofthisproduct.Ifanalternativemediumformulationisused,theATCCwarrantyforviabilityisno
800.638.6597or703.365.2700
longervalid.
Fax:703.365.2750
Email:Tech@atcc.org
Disclaimers
Orcontactyourlocaldistributor

Page1of2
 Thisproductisintendedforlaboratoryresearchpurposesonly.Itisnotintendedforuseinhumans.
WhileATCCusesreasonableeffortstoincludeaccurateanduptodateinformationonthisproductsheet,
ATCCmakesnowarrantiesorrepresentationsastoitsaccuracy.Citationsfromscientificliteratureand
patentsareprovidedforinformationalpurposesonly.ATCCdoesnotwarrantthatsuchinformationhasbeen
confirmedtobeaccurate.
Thisproductissentwiththeconditionthatyouareresponsibleforitssafestorage,handling,anduse.ATCC
ProductSheet
isnotliableforanydamagesorinjuriesarisingfromreceiptand/oruseofthisproduct.Whilereasonableeffort
Escherichiacoli(ATCC ismadetoinsureauthenticityandreliabilityofmaterialsondeposit,ATCCisnotliablefordamagesarisingfrom
themisidentificationormisrepresentationofsuchmaterials.
25922) PleaseseetheenclosedMaterialTransferAgreement(MTA)forfurtherdetailsregardingtheuseofthis
product.TheMTAisalsoavailableonourWebsiteatwww.atcc.org

PleasereadthisFIRST AdditionalinformationonthiscultureisavailableontheATCCwebsiteatwww.atcc.org.
ATCC2017.Allrightsreserved.ATCCisaregisteredtrademarkoftheAmericanTypeCultureCollection.[01/12]

StorageTemp.
Frozen:80Cor
colder

FreezeDried:2C
to8C
LiveCulture:See
Propagation
Section

BiosafetyLevel
1

IntendedUse

Thisproductisintendedforresearchuseonly.Itisnot
intendedforanyanimalorhumantherapeuticor
diagnosticuse.

CitationofStrain

Ifuseofthiscultureresultsinascientificpublication,it
shouldbecitedinthatmanuscriptinthefollowing
manner:Escherichiacoli(ATCC25922)

AmericanTypeCultureCollection
POBox1549
Manassas,VA20108USA
www.atcc.org

800.638.6597or703.365.2700
Fax:703.365.2750
Email:Tech@atcc.org

Orcontactyourlocaldistributor

Page2of2