Test Purpose Reagent Observation Results

Catalase To determine the Hydrogen Bubbles Positive

ability of bacteria to Peroxide
convert Hydrogen
peroxide to H2O, O2
Coagulase differentiate Staphyloc Plasma No clumps Negative
occus
aureus (positive) from
Coagulase Negative
Staphylococcus
(CONS)
Mannitol To determine if MSA None to few Negative
Salt Agar bacteria is able to fermentation
ferment mannitol

Mannitol Salt Agar (Specimen B) Staphylococcus Epidermidis

Observation: Few fermentation means it is negative

Catalase Test (Slide test)

Observations: It bubbled therefore it is
positive.
Coagulase Test: (Slide test)
Observations: No clumps therefore it is
negative.