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Service Locations & Telephone Numbers

Congress Registration & Congress Management


Hall 4D Skybridge, WSCC Level 4
Phone: (206) 219-4501

Friday, June 10 1200 1700


Saturday, June 11 700 1830
Sunday, June 12 700 1830
Monday, June 13 730 1830
Tuesday, June 14 730 1830
Wednesday, June 15 730 1700

Exhibitor Registration & Information


Hall 4D Skybridge, WSCC Level 4
Phone: (206) 219-4501

Friday, June 10 800 1700


Saturday, June 11 800 1700
Sunday, June 12 800 1930
Monday, June 13 930 1900
Tuesday, June 14 930 1900
Wednesday, June 15 900 1430

Exhibit Management Desk


Exhibit Hall 4EF, WSCC Level 4
Phone: (206) 219-4502

Speaker-Ready Room
Room 400, WSCC Level 4
Phone (206) 219-4503

Friday, June 10 1200 1700


Saturday, June 11 730 1700
Sunday, June 12 730 1700
Monday, June 13 730 1700
Tuesday, June 14 730 1700
Wednesday, June 15 730 1700

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ISAC 2016 Program and Abstracts 31


Committee Meetings
All meetings and events are by invitation only and held at the Washington State Convention
Center (WSCC) and the Conference Center (TCC) unless specified otherwise.

Friday, June 10

ISAC Marylou Ingram Scholars Day

700 2000 Madison Ballroom A, Renaissance Hotel

Saturday, June 11

ISAC Council Meeting

715 915 Vista Room, Renaissance Hotel

ISAC Marylou Ingram Scholar Program Committee Meeting

930 1030 Vista Room, Renaissance Hotel

Cytometry Part A Editorial Board Meeting

1200 1330 Vista Room, Renaissance Hotel

Sunday, June 12

Scientific Communications Committee

700 800 Yakima 2, TCC Level 1

Associated Societies Luncheon

1245 1400 Chelan 2, TCC Level 2

Biosafety Committee Meeting

1245 1400 Skagit 1, TCC Lower Level

Live Education Task Force

1245 1400 Skagit 2, TCC Lower Level

Finance Committee

1245 1400 Yakima 2, TCC Level 1

Monday, June 13

Membership Services Committee Meeting

730 830 Yakima 2, TCC Level 1

Image Cytometry Content Task Force

730 830 Skagit 2, TCC Lower Level

32 ISAC 2016 Program and Abstracts


Congress Overview

Overview
Congress
All Congress activities will be held at the Washington State Convention Center and the Conference Center unless
noted otherwise.

Friday, June 10, 2016

Lectures
Special
700 2000 ISAC Marylou Ingram Scholars Day Renaissance Hotel
800 1700 Exhibitor Registration Hall 4D Skybridge, WSCC Level 4
1200 1700 Scientific Registration Hall 4D Skybridge, WSCC Level 4

Saturday
11 June
Saturday, June 11, 2016
700 1830 Scientific Registration Hall 4D Skybridge, WSCC Level 4

12 June
Sunday
800 1700 Exhibitor Registration Hall 4D Skybridge, WSCC Level 4
930 1100 Scientific Tutorial Sessions 1-5 Tahoma 1-5, TCC Level 3,
and Skagit 4, TCC Lower Level
1000 1200 ICCE Exam Skagit 3, TCC Lower Level
1200 1330 Scientific Tutorial Sessions 6-10 Tahoma 1-5, TCC Level 3,

Monday
13 June
and Skagit 4, TCC Lower Level
1345 1515 Scientific Tutorial Sessions 11-14 Tahoma 1-4, TCC Level 3
and Yakima 1, TCC Level 1
1530 1915 CYTO Innovation and Keynote Lecture Hall 4C, WSCC Level 4
1650 1700 Coffee Break Hall 4C Foyer, WSCC Level 4

Tuesday
14 June
Sunday, June 12, 2016
700 1830 Scientific Registration Hall 4D Skybridge, WSCC Level 4

Wednesday
15 June
800 1930 Exhibitor Registration Hall 4D Skybridge, WSCC Level 4
745 845 First Time Attendee Orientation Tahoma 4, TCC Level 3
900 1030 Opening Remarks and State of the Art Lectures Hall 4C, WSCC Level 4
1030 1100 Coffee Break Hall 4C Foyer, WSCC Level 4
1100 1230 Concurrent Parallel Sessions 1-5 Tahoma 1-5, TCC Level 3,

Session
Poster
and Skagit 4, TCC Lower Level
1245 1345 Commercial Tutorials Tahoma 1-5, TCC Level 3,
and Skagit 4, TCC Lower Level
1415 1530 Concurrent Workshops 1-5 Tahoma 1-5, TCC Level 3,
and Skagit 4, TCC Lower Level

Scholars &
Emerging
Leaders
1530 1545 Coffee Break Tahoma Foyer, TCC Level 3
and Skagit Foyer, TCC Lower Level
1545 1700 Concurrent Workshops 6-10 Tahoma 1-5, TCC Level 3,
and Skagit 4, TCC Lower Level
1715 1815 Robert Hooke Lecture Hall 4C, WSCC Level 4

Commercial
Tutorials &
Exhibits
1600 2000 Poster Set-up Exhibit Hall 4EF, WSCC Level 4
1800 2000 Commercial Exhibits Exhibit Hall 4EF, WSCC Level 4
1830 1930 Opening Reception Exhibit Hall 4EF, WSCC Level 4
Oral Session
Monday, June 13, 2016
Abstracts

730 1830 Scientific Registration Hall 4D Skybridge, WSCC Level 4


800 1930 Poster Viewing Exhibit Hall 4EF, WSCC Level 4
Poster Session

900 1030 Frontiers Session 1 Hall 4C, WSCC Level 4


Abstracts

930 1900 Exhibitor Registration Hall 4D Skybridge, WSCC Level 4


1030 1100 Coffee Break Exhibit Hall 4EF, WSCC Level 4
1030 1930 Commercial Exhibits Exhibit Hall 4EF, WSCC Level 4
1100 1230 Concurrent Parallel Sessions 6-10 Tahoma 1-5, TCC Level 3,
Speaker/Author

and Skagit 4, TCC Lower Level


1245 1345 President's Award for Excellence Exhibit Hall 4EF, WSCC Level 4
Index

Presentations

ISAC 2016 Program and Abstracts 35


1245
 1345 Commercial Tutorials Tahoma 1-5, TCC Level 3,
Overview
Congress

and Skagit 4, TCC Lower Level


1415
 1505 Plenary Session 1A Hall 4C, WSCC Level 4
1505 1535 Coffee Break Exhibit Hall 4EF, WSCC Level 4
1535 1625 Plenary Session 1B Hall 4C, WSCC Level 4
1645 1800 Concurrent Workshops 11-15 Tahoma 1-5, TCC Level 3,
Lectures
Special

and Skagit 4, TCC Lower Level


1800 1900 Poster Session 1 Exhibit Hall 4EF, WSCC Level 4
1830 1930 Happy Hour Exhibit Hall 4EF, WSCC Level 4
1830 1930 Exhibitor Showcase Exhibit Hall 4EF, WSCC Level 4
1930 2230 Shared Resource Lab Forum Hall 4C, WSCC Level 4
Saturday
11 June

Tuesday, June 14, 2016


730 1830 Scientific Registration Hall 4D Skybridge, WSCC Level 4
12 June
Sunday

800 2000 Poster Viewing Exhibit Hall 4EF, WSCC Level 4


900 1030 Frontiers Session 2 Hall 4C, WSCC Level 4
930 1900 Exhibitor Registration Hall 4D Skybridge, WSCC Level 4
1030 1100 Coffee Break Exhibit Hall 4EF, WSCC Level 4
1030 2000 Commercial Exhibits Exhibit Hall 4EF, WSCC Level 4
Monday
13 June

1100 1230 Concurrent Parallel Sessions 11-15 Tahoma 1-5, TCC Level 3,
and Skagit 4, TCC Lower Level
1245 1345 Exceptional Student Award Exhibit Hall 4EF, WSCC Level 4
Presentations
1245 1345 Commercial Tutorials Tahoma 1-5, TCC Level 3,
Tuesday
14 June

and Skagit 4, TCC Lower Level


1415 1530 Plenary Session 2 Hall 4C, WSCC Level 4
1530 1600 Coffee Break Exhibit Hall 4EF, WSCC Level 4
1600 1715 Concurrent Workshops 16-20 Tahoma 1-5, TCC Level 3,
Wednesday

and Skagit 4, TCC Lower Level


15 June

1730 1830 Keynote Lecture Hall 4C, WSCC Level 4


1830 1930 Poster Session 2 Exhibit Hall 4EF, WSCC Level 4
1900 2000 Happy Hour Exhibit Hall 4EF, WSCC Level 4
1900 2000 Exhibitor Showcase Exhibit Hall 4EF, WSCC Level 4
Session
Poster

Wednesday, June 15, 2016


730 1700 Scientific Registration Hall 4D Skybridge, WSCC Level 4
800 1415 Poster Viewing Exhibit Hall 4EF, WSCC Level 4
Scholars &
Emerging
Leaders

830 900 ISAC Business Meeting Hall 4C, WSCC Level 4


900 1030 Frontiers Session 3 Hall 4C, WSCC Level 4
900 1430 Exhibitor Registration Hall 4D Skybridge, WSCC Level 4
1030 1430 Commercial Exhibits Exhibit Hall 4EF, WSCC Level 4
Commercial

1030 1100 Coffee Break Exhibit Hall 4EF, WSCC Level 4


Tutorials &
Exhibits

1100 1200 Poster Highlight Tour Exhibit Hall 4EF, WSCC Level 4
1100 1230 Concurrent Parallel Sessions 16-20 Tahoma 1-5, TCC Level 3,
and Skagit 4, TCC Lower Level
1245 1345 Commercial Tutorials Skagit 4, TCC Lower Level
Oral Session

1410 1500 ICCS/ESCCA Guest Symposium A Hall 4C, WSCC Level 4


Abstracts

1500 1530 Coffee Break Hall 4C Foyer, WSCC Level 4


1530 1620 ICCS/ESCCA Guest Symposium B Hall 4C, WSCC Level 4
1630 1730 Awards Ceremony Hall 4C, WSCC Level 4
1900 2300 Closing Reception Seattle Aquarium
Speaker/Author Poster Session

(Must present ticket for admittance.)


Index Abstracts

36 ISAC 2016 Program and Abstracts


Overview
Congress
Visit the Exhibits & Posters

Lectures
Special
Sunday, June 12
1600 2000 Authors Must Place Posters on Assigned Boards
1800 2000 Commercial Exhibits Open

Saturday
11 June
Monday, June 13
800 1930 Poster Viewing
1030 1100 Coffee Break
1030 1930 Commercial Exhibits

12 June
Sunday
1800 1900 Poster Session I (Authors of odd numbered boards present)
1830 1930 Happy Hour
1830 1930 Exhibitor Showcase

Monday
Tuesday, June 14

13 June
800 2000 Poster Viewing
1030 1100 Coffee Break
1030 2000 Commercial Exhibits
1830 1930 Poster Session II (Authors of even numbered boards present)

Tuesday
14 June
1900 2000 Happy Hour
1900 2000 Exhibitor Showcase

Wednesday, June 15

Wednesday
15 June
800 1415 Poster Viewing
1030 1100 Coffee Break
1030 1430 Commercial Exhibits
1100 1200 Poster Session III (consists of Poster Highlights Tour)

Session
Poster
Posters must be removed by 1430 on Wednesday, June 15, 2016.

Scholars &
Emerging
Leaders

Commercial
Tutorials &
Exhibits
Dont forget to exchange your Closing Reception coupon for a ticket to the
Tuesday evening Closing Reception at the Seattle Aquarium!
Oral Session
Abstracts

Coupons will be exchanged on a first come, first served basis until Sunday, June
12, or until the maximum capacity is reached.
Poster Session
Abstracts

(Ticket required for admittance; see page 23 for details.)


Speaker/Author
Index

ISAC 2016 Program and Abstracts 37




 Special Lectures
Overview
Congress

Saturday,
 June 11
1745 1845
CYTO Innovation Keynote
Advancing the Art and Science of Flow Cytometry through Advanced Materials
Lectures
Special

Brent Gaylord, BD Life Sciences, USA


Saturday
11 June
12 June
Sunday

Dr. Gaylord received his Ph.D. in material sciences from the University of California at Santa Barbara (UCSB) for his work in the
Development and Biological Application of Light Emitting Conjugated Polymers. This research ultimately led to the development
of Sirigen's core technology and a broad IP portfolio. After cofounding Sirigen he served as the companys Chief Scientific Officer
Monday
13 June

for eight years until the company was acquired in 2012 by Becton Dickinson (BD). He currently serves as a Director of R&D
within BD Biosciences, responsible for the development of life science research reagents and the Sirigen technology
platform. Prior to attending UCSB, he obtained a B.S. in Chemistry-Material Science Engineering and a minor in Mathematics
from the United States Air Force Academy.
Tuesday
14 June

Sunday, June 12
1715 1815
Robert Hooke Lecture
Imaging of Induced Pluripotent Stem Cells
Rick Horwitz, Allen Institute for Cell Science, USA
Wednesday
15 June
Session
Poster
Scholars &
Emerging
Leaders

Rick Horwitz is the inaugural Executive Director of the Allen Institute for Cell Science in Seattle. Previously, he was a University
Professor and Harrison Distinguished Professor of Cell Biology at the University of Virginia. He also served as the Director of the
Cell Migration Consortium: an NIH-funded multi-institutional, multi-disciplinary collaboration. Previously, he served as the Head
Commercial

of the nascent Department of Cell and Developmental Biology at the University of Illinois and was a Professor of Biophysics and
Tutorials &
Exhibits

Physical Biochemistry at the University of Pennsylvania. Rick earned his B.A. in the Honors Program at UW Madison, majoring in
chemistry (math, physics). He received his Ph.D. in Biophysics at Stanford University and did postdoctoral research in NMR at UC
Berkeley. He has served on numerous strategic planning and advisory committees and organized several international meetings.
Rick is known for his pioneering and leadership roles in cell adhesion, migration, and live-cell imaging.
Oral Session
Abstracts
Speaker/Author Poster Session
Index Abstracts

38 ISAC 2016 Program and Abstracts


open to Full Congress Registrants; no additional fees

Overview
1345 1515, Tahoma 1 TCC Lower Level

Congress
apply.
SCIENTIFIC TUTORIAL 11
AGENDA
1345 11
Imaging Bioinformatics for High Content Screening 1530
Introduction

Lectures
of 3D Cell Culture Models

Special
B. Parvin. Univ. of Nevada, Reno, U.S.A. 1540 14
The Journey from Concept to Commercialization: A
1345 1515, Tahoma 3, TCC Level 3 Daunting Task with High Rewards for Entrepreneurs
J. Phi. IntelliCyt, Albuquerque, U.S.A.
SCIENTIFIC TUTORIAL 12

Saturday
11 June
1610
1345 12 Technology Showcase Presentations
Flow Cytometry Biosafety
Judges:
K. Holmes, H. Pletcher, S. Monard and S.P. Perfetto.
NIAID, NIH, Univ. of Pennsylvania, U.S.A. and Joe Victor, Paul Smith, and David Basiji

12 June
Sunday
Walter & Eliza Hall Inst. of Med. Res., Australia 1650
Break
1345 1515, Tahoma 4, TCC Level 3 1700
SCIENTIFIC TUTORIAL 13 Technology Showcase Presentations

Monday
13 June
1345 13 Judges:
Microfluidic Flow Cytometry and Cell Sorting Joe Victor, Paul Smith, and David Basiji
T. Huang. Penn State, U.S.A. 1730
Break

Tuesday
14 June
1345 1515, Yakima 1, TCC Level 1 1745 15
SCIENTIFIC TUTORIAL 14 CYTO Innovation Keynote
Introduction: Allen Poirson
1345 448 Advancing the Art and Science of Flow Cytometry

Wednesday
Introducing Cytometry through Hands-On Activities

15 June
through Advanced Materials
J.P. Hill, A. Conway, D. Callen, R. Weeks, T. B. Gaylord. Becton Dickinson, San Diego, U.S.A.
Caraballo, J. Chalupny and S. Friend. NIAID, NIH,
Roswell Park Cancer Inst., Buffalo, Coherent Inc., 1830
Wilsonville, OR, Shoreline Community Col., Seattle, Networking Session
Glacier Peak H.S. Shoreline and Amnis/EMD 1915

Session
Poster
Millipore, Seattle, U.S.A. Session Ends

1530 1915, Hall 4C, WSCC Level 4 1650 1700, Hall 4C Foyer, WSCC Level 4
CYTO INNOVATION AND KEYNOTE LECTURE COFFEE BREAK

Scholars &
Emerging
Leaders
Chairs: Diether Recktnewald, Betsy Ohlsson-Wihelm
and Jonni Moore
The advancement of cytometry depends on the SUNDAY, JUNE 12, 2016

Commercial
translation of innovative research into useful,

Tutorials &
Exhibits
accessible tools that can catalyze new biological 745 845, Tahoma 4, TCC Level 3
understanding. CYTO Innovation is the forum within
ISAC that explores the challenges and opportunities CYTO FIRST TIME ATTENDEE WELCOME &
for translation of new cell analysis technologies into ORIENTATION Oral Session
commercially viable products and services. CYTO
Abstracts

Is this your first time attending CYTO? ISAC


Innovation provides: an opportunity to learn about the Welcomes you to its 31st International Congress
translation of ideas and discoveries into products and CYTO 2016!
knowledge; the skills required for progressing from a All first time attendees as well as nonmember
research finding to useful products with impact; a
Poster Session

registrants are invited to a Welcome & Orientation.


Abstracts

unique platform for showcasing innovation in relevant New attendees are welcome to join the CYTO family
industry sectors; and engagement with a for continental breakfast and to meet new friends. The
knowledgeable network of entrepreneurs, executives session will be informal and friendly with the
and other business people. CYTO Innovation is part of opportunity to meet with old timers of the CYTO
ISACs wider mission of advancing cytometry and conferences as well as other first time attendees.
Speaker/Author

aims to serve members and Congress attendees either During the event, you will meet ISAC Council
Index

engaged in or contemplating the translation of members, Cytometry Part A Editor-in-Chief, the


research into high impact products and services. It is

ISAC 2016 Program and Abstracts 41


Membership Services Committee (MSC) members, 1140 21
Overview
Congress

and other leaders of ISAC. Peter Lopez, ISAC Council Simultaneous Determination of mRNA and Protein
member and chair of the MSC, will make opening Expression Profiles of T-bet and Eomes in Stimulated
remarks, and representatives from the ISAC Scholars T Lymphocytes by Flow Cytometry
and Shared Resource Lab Emerging Leaders programs K.T. Soh, J.D. Tario, Jr. and P.K. Wallace. Roswell
will speak about their first CYTOs and advice they Park Cancer Inst., Buffalo, NY, U.S.A.
Lectures
Special

have to share. After the formal presentation, attendees


will be encouraged to network with each other and 1200 32
ISAC Leaders, who will be sitting at different tables Detection of HIV RNA Expression in Peripheral
throughout the room. Blood from HIV-Infected Study Participants by
There will be opportunity to ask questions; find out RNAflow Cytometry
Saturday
11 June

the resources available at the conference; bring up C. Baker, A. Tan, J. McCune, E. Sinclair and J. Milush.
specific topics of interest and request to be put in UCSF, U.S.A.
contact with specific cytometrists who will also be
attending CYTO 2016. This event is sponsored by 1100 1230, Tahoma 3, TCC Level 3
Cytometry Part A, ISACs Journal.
12 June
Sunday

PARALLEL 2: MICROFLUIDIC CYTOMETRY


900 1030, Hall 4C, WSCC Level 4 Chair: Timothy Bushnell
Cochair: Alfonso Blanco Fernandez
OPENING REMARKS & STATE-OF-THE-ART
LECTURES 1100 23
Monday
13 June

Label-Free Separation of Neuroblastoma Tumor Cells


Chair: John Nolan from Mononuclear Cells Using an Acoustophoresis-
Cochair: Alan Waggoner Based Microfluidic Chip
900 16 F. Olm, A. Urbansky, T. Laurell and S. Scheding. Lund
Quantitative Physiology of Living Single-Cells: Subtle Univ., Sweden
Tuesday
14 June

Pleasures and Future Promise 1120 24


R. Brent. Fred Hutchinson Cancer Res. Ctr., Seattle, Microfluidic Post Geometry Design for High-
U.S.A. Throughput (100 L/min) Harvesting of Leukocytes
930 17 from Blood Using Deterministic Lateral
Wednesday
15 June

High Content Analysis of Pancreatic Cancers Using Displacement Arrays


Imaging Mass Cytometry J. D'Silva, R. Austin and J. Sturm. Princeton Univ.,
D. Hedley. Princess Margaret Hosp., Toronto, Canada U.S.A.
1000 18 1140 25
Nanotechnology Platforms for Extracellular Vesicle A Time-Resolved Microfluidic Flow Cytometer and
Session
Poster

Analysis Sorter for FRET-Based Biosensors


H. Lee. Massachusetts Gen. Hosp., U.S.A. B. Fiedler, S. Van Buskirk, A. Palmer and R. Jimenez.
Univ. of Colorado Boulder and JILA/NIST, Boulder,
1030 1100, Hall 4C Foyer, WSCC Level 4 U.S.A.
Scholars &
Emerging
Leaders

COFFEE BREAK 1200 26


A High Throughput Bubble Jet Microfluidic FACS
C. Liu, D. Vercruysse, K. de Wijs, A. Dusa, S. Mao, K.
1100 1230, Tahoma 1, TCC Level 3
Blaszkiewicz, Y. Li, B. Majeed, X. Rottenberg, D.
Commercial

PARALLEL 1: RNA FLOW CYTOMETRY


Tutorials &

Sabuncuoglu Tezcan, P. Deshpande and L. Lagae.


Exhibits

Chair: David Hedley imec, Leuven and Katholieke Univ. Leuven, Belgium
Cochair: Benjamin Daniel
1100 19 1100 1230, Tahoma 4, TCC Level 3
Oral Session

Highly Multiplexed Simultaneous Detection of PARALLEL 3: IMMUNOLOGY


Abstracts

Proteins and RNAs in Single Cells


Chair: Zofia Maciorowski
P.F. Gherardini, A.P. Frei, F.A. Bava, E. Zunder,
Cochair: Anna Belkina
E.W.Y. Hsieh, S-Y. Chen and G.P. Nolan. Stanford
Univ., Univ. of Virgina and Univ. of Colorado 1100 27
Speaker/Author Poster Session
Abstracts

Denver, U.S.A. Generation of Stem Cell-Like Memory T Cells from


1120 20 Naive Precursors for Adoptive Cell Transfer
Simultaneous Imaging of Proteins and Transcripts in Immunotherapy
Cells by Highly Multiplexed Imaging Mass Cytometry A. Roberto, V. Zanon, K. Pilipow and E. Lugli. Human
D. Schulz, S. Chevrier, H. Jackson, Z. Varga and B. Clin. and Res. Ctr., Rozzano, Milan, Italy
Index

Bodenmiller. Univ. of Zurich and University Hosp.


Zurich, Switzerland

42 ISAC 2016 Program and Abstracts


ISAC Marylou Ingram Scholars

Overview
Congress
The ISAC Marylou Ingram Scholars program is designed to at the University of Rochester, Caltech and the University of
enhance the scientific and leadership experiences of Miami; at Los Alamos National Laboratory; and as a
emerging leaders in the field of cytometry. It is a career consultant to the National Cancer Institute, the FDA,
development program for those under the age of 40 on the Brookhaven National Laboratory, NASA and other

Lectures
Special
date of the application. The program provides opportunities organizations. She was a pioneer in automated cell analysis,
for leadership training, an opportunity to apply for a mentor played a key role in developing automated cell analysis
training opportunity with another Society member, systems, and was founding director of the Institute for Cell
presentation opportunities, financial support for Analysis at the University of Miami. Dr. Ingram spent two
membership in ISAC, as well as other valuable mentoring of her sabbaticals working directly with Wallace Coulter in

Saturday
11 June
activities. In addition, ISAC Marylou Ingram Scholars are the very early days of automated cell analysis, and she
given the opportunity to be an integral part of the Society regularly interacted with Wallace Coulter as he developed
through committee memberships, advisory boards, the new technologies for analysis. From 1982 to 2013, she was
opportunity to review manuscripts submitted to Cytometry, a Senior Research Scientist at the Huntington Medical
Part A (the official journal of ISAC) and creating educational Research Institute in Pasadena, California. She headed the
material to further support the goals of ISAC. Being chosen Tissue Engineering & In Vitro Systems program and led a

12 June
Sunday
as an ISAC Marylou Ingram Scholar is an indication that you research program on tumor spheroids with the aim of
are recognized for your leadership potential, scientific skills creating better models for studying tumor growth and drug
and ability to achieve your career goals. responsiveness.

ISAC recently received a grant from the Wallace H. Coulter One of the ways this grant will be used to support and

Monday
13 June
Foundation to support and strengthen the ISAC Scholar strengthen the ISAC Marylou Ingram Scholars program is by
Program. As of receipt of the grant and going forward, the paying for one day of activities for ISAC Marylou Ingram
ISAC Scholar program shall be known as the ISAC Marylou Scholars before the start of CYTO, ISACs annual Congress.
Ingram Scholars Program, and current and future Scholars The first ISAC Marylou Ingram Scholars day will be on
should refer to themselves as ISAC Marylou Ingram Friday, June 10, 2016.
Scholars.

Tuesday
14 June
Marylou Ingrams contribution to the field of cytometry
spanned nearly 70 years. Dr. Ingrams distinguished career
in academic medicine and research included time as faculty

Wednesday
ISAC Marylou Ingram Scholars 2012-2016

15 June
Participation/Support of ISAC
Pratip K. Chattopadhyay Within ISAC, I have been involved in a number of activities,
Staff Scientist, ImmunoTechnology including developing tutorials and day-long pre-congress

Session
Poster
Section, Vaccine Research Center, courses on mass cytometry, data analysis, and
NIH, Bethesda, Maryland, USA polychromatic cytometry. I am currently a member of the
Education Education Committee, and I lead the Electronic Learning (e-
Bachelors of Arts, Biology: University learning) Task Force. As part of this group, I played a key
of Virginia role in the early development of our on-line education

Scholars &
Emerging
Leaders
Ph.D., Molecular Microbiology and portal (CYTO U), and I currently manage the electronic
Immunology: Johns Hopkins delivery/production of new CYTO U courses. As an ISAC
University School of Public Health Scholar, my colleague Nima Aghaeepour and I applied for
Postdoctoral Training: National Institutes of Health (and won) funding for the first Scholar Collaboration
Scientific Interest and Avenues for Collaboration project, and have developed a proposal for more high-

Commercial
Tutorials &
profile presentations of Scholar work at the CYTO meeting.

Exhibits
My work aims to identify the characteristics of T- and B-
cells that predict successful immune responses. For this
work, I develop and optimize multi-parametric proteomic Enrico Lugli
and transcriptomic technologies, collaborating closely with Principal Investigator, Laboratory of
engineers and bioinformaticians. I then apply these new Translational Immunology Oral Session
Abstracts

technologies to better understand basic T-cell immunology Head, Humanitas Flow Cytometry Core
and the immune response to vaccines or natural disease, Humanitas Clinical and Research
using large cohorts of individuals with well-defined clinical Center
outcomes (particularly in HIV and EBV-associated diseases). Rozzano, Italy
I have extensive experience collaborating with groups 1) Education
Poster Session

developing new hardware platforms or reagents, 2) Ph.D., University of Modena and


Abstracts

searching for settings to test their data analysis tools, and 3) Reggio Emilia, Italy (supervisor: Prof. Andrea Cossarizza)
clinicians or epidemiologists interested in comprehensive Post-doctoral fellow, ImmunoTechnology Section, Vaccine
characterization of immune responses. I am always eager Research Center, NIAID, NIH, Bethesda, MD USA
to work with new collaborators in these areas. (supervisor: Dr. Mario Roederer)
Speaker/Author
Index

ISAC 2016 Program and Abstracts 55



Scientific
 Interest and Avenues for Collaboration Qianjun Zhang
Overview
Congress

x Mechanisms of memory T cell differentiation and General Manager, Emerald Biotech,


 maintenance in humans located in Hangzhou, China
x Cytokine-based cancer immunotherapy Education
x Immune reconstitution in acquired M.S., University of Colorado Health
immunodeficiencies (bone marrow Sciences Center
Lectures
Special

transplantation, HIV infection) Scientific Interest and Avenues for


Participation/Support of ISAC Collaboration
 Organization committee of CYTO 2014 I am enthusiastic about the new
 Attended CYTO 2007, 2012, 2013, 2014 technology within flow cytometry and
 ISAC-sponsored flow cytometry workshop @ ESID also dedicated to enhancing the training and knowledge of
flow techniques within resource limited areas such as South
Saturday
11 June

(Prague, Nov. 1-2, 2014)


East Asia and China. I have helped organize a few
 Cytometry Part A Editorial Board
international flow cytometry workshops and served as
 Member of the Scientific Communications faculty teaching at those venues. I would like to continue
Committee contributing and help the educational committee with my
resource and expertise.
12 June
Sunday

Gergely Toldi Participation/Support of ISAC


clinical fellow, Birmingham Women's ISAC Live Education Delivery Task Force member (2013
Hospital, Birmingham, UK present)
assistant professor, First Dept. of Actively involved in CYTO 2012, 2014
Obstetrics and Gynecology, Semmelweis Co-Chaired Parallel Sessions at CYTO 2015
University, Budapest
Monday
13 June

Serve as abstract reviewer for CYTO 2016


Education Website
MD (2011), PhD (2012), Board www.linkedin.com/in/qianjunzhang/
Certification in Pediatrics (2015),
Specialty Training in Neonatology (2015-present)
Nima Aghaeepour
Scientific Interest and Avenues for Collaboration Postdoctoral Fellow, Stanford
Tuesday
14 June

T lymphocyte activation, kinetic flow cytometry, University


autoimmunity, immunology of pregnancy and neonates Education Ph.D., Bioinformatics,
Participation/Support of ISAC University of British Columbia, 2008-
CYTO 2013, 2014, 2015 & 2016: Program Committee 2012 B.Sc., Computer Science,
Member, Session Chair University of Tehran, 2003-2008
Wednesday

Outstanding Poster Award, CYTO 2012


15 June

Scientific Interest and Avenues for


Reviewer for Cytometry A Collaboration: Nima Aghaeepour is
currently a postdoctoral fellow in Garry
Michael Zordan Nolans laboratory at Stanford University, a Fellow of the
Staff Engineer, Sony Biotechnology, Canadian Institute of Health Research, and an Ann
Inc. Schreiber Mentored Investigator of the Ovarian Cancer
Session

San Jose, CA
Poster

Research Fund. His research is focused on computational


Education analysis of single cell proteomics and genomics data to
Duke University B.S.E. Biomedical enable a systems level understanding of complex cancers as
Engineering well as hematologic and immune system malignancies.
Purdue University. Ph.D. Biomedical Participation/Support of ISAC
Scholars &

Engineering
Emerging
Leaders

He currently contributes to ISAC as a member of the finance


Post-Doctoral Fellow, Purdue and CYTO innovation committees and was a former of the
University, Laboratory of Prof. J. Paul Robinson scientific communications committee and the CYTO
Scientific Interest and Avenues for Collaboration conferences program committee. He has also chaired
My research interests are in the design and development of several CYTO parallel sessions, has co-organized the
Commercial

novel cell analysis instruments and technology. I would be


Tutorials &

FlowCAP parallel sessions, workshops, and tutorials, and


Exhibits

interested in collaborating with anyone interested in was an invited faculty in ISACs pre-congress advanced data
developing their technology or with researchers to design analysis course.
technology to perform novel measurements they cannot Website: http://linkedin.com/in/naghaeep
currently perform.
Participation/Support of ISAC Anis Larbi
Oral Session
Abstracts

Member of ISAC Data Standards Task Force Principal Investigator, Immunity and
Co-Chaired Parallel Sessions at CYTO 2013 and CYTO Aging Program, Singapore Immunology
2014 Network (SIgN) Director, A*STAR Flow
Invited Speaker, GLIIFCA 2013 Cytometry Platform Associate
Invited Speaker, Metroflow 2014 Professor, Department of Geriatrics,
Speaker/Author Poster Session

Faculty of Medicine, University of


Abstracts

Sherbrooke, Canada
Education
2005 - PhD Immunology: Research
Center on Aging, Laboratory of immunology and signaling,
Sherbrooke, Faculty of Medicine, University of Sherbrooke,
Canada
Index

56 ISAC 2016 Program and Abstracts


HEMATOLOGICAL DISORDERS

Overview
Congress
B2 139
Multimedia and Poster Sessions Automated Identification of Novel Cell Types
Basedupon Signaling Characteristics in Chronic
Exhibit Hall 4EF, WSCC Level 4 Myeloid Leukemia

Lectures
Special
J. De and P. Kane. Deepath Med. Diagnostics, Palo
Author presentation and discussion times: Alto, U.S.A.
Sunday, June 12 B3 140
1600 2000 Authors must set up posters on Survival Stratification in Acute Myeloid Leukemia by
Single Cell Signal Profiling

Saturday
assigned board

11 June
J. Skavland, S-E. Gullaksen, S. Gavasso, H. Reikvam,
Monday, June 13 . Bruserud and B.T. Gjertsen. Univ. of Bergen and
800 1930 Poster Viewing Haukeland Univ. Hosp., Norway
1800 1900 Poster Session I: Authors of ODD
numbered poster boards present NEW SOFTWARE DEVELOPMENT

12 June
Sunday
B4 141
Tuesday, June 14 CyTOF Studio: Automated Classificationand Analysis
800 2000 Poster Viewing of High-Dimensional Mass Cytometry Data
1830 1930 Poster Session II: Authors of EVEN
S. Davis, M. Nolan and J. Hill. GlaxoSmithKline,
numbered poster boards present

Monday
13 June
U.S.A.
Wednesday, June 15 OTHER BIOLOGICAL APPLICATIONS
800 1415 Poster Viewing
B5 142
1100 1200 Poster Session III (Consists of Poster
Highlights Tour) Flow and Image Cytometry Educational Outreach

Tuesday
14 June
1430 All poster material must be removed Opportunities: Roswell Park Cancer
from the boards Institute Experience
A. Conway, A. Kisailus, O. Maguire, J.D. Tario, E.
Podniesinski, H. Minderman and P.K. Wallace.

Wednesday
Roswell Park Cancer Inst., Buffalo, U.S.A.

15 June
The number listed following the letter B
signifies the poster board number/location in
the Exhibit Hall 4EF on WSCC Level 4. The
POSTER PRESENTATIONS
number next to the board number signifies the
ANTIGEN-SPECIFIC IMMUNE RESPONSES

Session
program number which can be referenced to

Poster
the abstract located in the back of the B6 143
program book. A Fluorescent Cell Barcoding Method to Measure
Human CTL Responses: Development of a "Cytotox-
Flow" Approach for Small Samples of Living Cells

Scholars &
Emerging
Leaders
F-E. L'Faqihi, M. Farc, S. Mueller, D. Lestrade and S.
MULTIMEDIA PRESENTATIONS Valitutti. INSERM U1043, Toulouse, France
B7 144
CELL SORTING AND SELECTION Recombinant BCG Over Expressing Ag85C Generates

Commercial
Tutorials &
Exhibits
B1 138 Durable Central Memory T Cells and Showed
Pre-enrichment of ILC2 from Whole Blood Reduces Enhanced Anti-mycobacterial Activity in Cattle
Sort Time and Results in Improved Purity A. Kumar and H. Krishna Prasad. All India Inst. of
J. Wilshire, Y. Valdez, S. Kyei, G. Poon, F. Takei, C. Med. Sci., New Delhi, India
Oral Session
AUTOMATED MICROSCOPY
Abstracts

Peters, S. Woodside, A. Eaves and T. Thomas.


STEMCELL Technols. and Terry Fox Lab., Vancouver,
B8 145
Canada
Interest of Raman Microspectroscopy in the
FLOW CYTOMETRY INSTRUMENTATION Diagnosis and Prognosis of B-Chronic Lymphocytic
Poster Session

B312 449 Leukemia


Abstracts

In Memorium: Dick Sweet and His Contributions to N. Bailly, D. Gheldof, S. Walbrecq, L. Liu, M. Fere, C.
Flow Cytometry and Cell Sorting Gobinet, J. Clossat, E. Cornet, X. Troussard, F. Mullier
M. Bigos, D. Houck. Stanford Univ. and BD and B. Chatelain. Catholic Univ. of Louvain, Belgium,
Biosciences (retired), U.S.A. Univ. of Reims Champagne-Ardenne, TRIBVN,
Speaker/Author

Chatillon and CHU Caen, France


Index

ISAC 2016 Program and Abstracts 69


AUTOMATED SAMPLE PREPARATION B16 153
Overview
Congress

B9 146 Issues with Mesenchymal Stem Cell Markers in


Car Wash an Integrated Continuous-Flow Amniotic Cells
Microfluidic Device to Prepare Leukocytes for Flow W. Weston, R. Frisch and S. Namin. UMTB/Vivex
Cytometry by Deterministic Lateral Displacement Biomed., Miami, U.S.A.
Lectures
Special

Y. Chen, J.L. DSilva, R.H. Austin, J.C. Sturm, X. Chen, B17 154
K. Reeder, C. Civin, T. Ward, A. Skelley, K. Gandhi, L. Mass Cytometry: TNF Signaling in Patients with
Aurich, Z.P. Lee and C. Dosier. Princeton Univ., Rheumatoid Arthriti
Univ. of Maryland Sch. of Med. and GPB Scientific, L. Bader, S-E. Gullaksen, J. Skavland, C. Vedeler, C.
Richmond, VA, U.S.A. Gram Gjesdal and S. Gavasso. Haukeland Univ.
Saturday
11 June

B10 147 Hosp. and Univ. of Bergen, Norway


Automated Processing of Human Blood Leukocytes B18 155
by a Plastic, Disposable Microfluidic Deterministic NIST Measurement Service for ERF Value
Lateral Displacement Device Assignments of Calibration Microspheres in Flow
12 June
Sunday

A. Skelley, T. Ward, K. Gandhi, Z.P. Lee, C. Dosier, Cytometry


J.L. DSilva, Y. Chen, M. Kim, X. Chen, L. Aurich, D. P. DeRose, A. Gaigalas and L. Wang. NIST,
Recktenwald, R.H. Austin, M. Grisham, C. Civin and Gaithersburg, MD, U.S.A.
J.C. Sturm. GPB Scientific, Richmond, VA, Princeton B19 156
Univ. and Univ. of Maryland Sch. of Med., U.S.A. Development, Validation, and Outsourcing of a Flow
Monday
13 June

B11 148 Cytometry Assay to Measure Pharmacodynamic


Preparation of Polychromatic Antibody Cocktails for Response in Patients with B-Cell Malignancies
Immunophenotypic Whole Blood Assays Using a Treated with BTCT4465A, an Anti-CD20/CD3 T-Cell
Semi-automated Approach Dependent Bispecific Therapy
Tuesday
14 June

I. Gonzalez, T. Meeuwsen, W. Miller, D. Haley, V. K. Dalpozzo, C. Green, G. Hernandez, C. Marathe


Conrad, T. Poplonski, A. Tanibata-Branham, K. Bahjat and J. Wallin. Genentech, South San Francisco,
and Y. Koguchi. Earle A. Chiles Res. Inst., Portland, U.S.A.
OR, Sony Biotechnol., San Jose, Beckman Coulter, B20 157
Inc. Life Sci., Brea, CA, Bristol-Myers Squibb,
Wednesday

Detection of PDL1 and PDL2 on Circulating Tumor


15 June

Redwood City, CA, U.S.A. Cells Using Microfluidic Based Chipcytometry


BIOMARKERS J. Teo, M. Tan, A. Mirenska, J. Oh, L. Hong, A.
B12 149 Bhagat, R. Wnek, J. Detmers, C-L. Chin and D.
Automated Analyses of Mass Cytometry Data Enables Skibinski. MSD Intl. Singapore, ClearBridge
Biomedics, Singapore, Zellkraftwerk GmbH,
Session
Poster

Identification of Biomarkers That Associate with


Hannover, Germany, Merck & Co. Inc., Rahway,
Manufacturing Success for CAR-T Cell Therapies
U.S.A and Zellkraftwerk GmbH, Leipzig, Germany
I. Pruteanu-Malinici, P. Pierog, A. Colantonio, A.
Veloso and S. Kassim. Novartis, Cambridge, MA, B21 158
U.S.A. Performing a Fit for Purpose Validation Exercise of
Scholars &
Emerging
Leaders

Flow Cytometry Assays for Lymphocyte Populations


B13 150
Assessed as Pharmacodynamic Biomarkers during
Gene Expression Profiling and Molecular
Drug Development Clinical Trials
Characterization of Miltefosine Resistance in L.
D. Lanham and M. Patel. Eurofins Pharma Bioanalysis
donovani.
Commercial
Tutorials &

UK Ltd, Abingdon, U.K.


Exhibits

P. Tiwary, D. Kumar and S. Sundar. Banaras Hindu


Univ., India B22 159
Development and Validation of a Clinical Flow
B14 151
Cytometry Assay to Measure Intracellular Ki67
Optimal Detection of Urinary Extracellular Vesicles
Oral Session

Expression in Memory T Subsets, NK and Treg Cells


Abstracts

in Evaluating Chronic Kidney Disease


in Human Whole Blood
M. Mizus, E. Mohler, III, R.D. Schretzenmair, L.
Y. Sun, T. Bridal, K. Yang, L. Patti-Diaz, M.N.
Zhang, W.T. Rogers, L.B. Holzman, R.R. Townsend
Hedrick, M. Cleary and A.G. Ehrhardt. Bristol-Myers
and J.S. Moore. Perelman Sch. of Med., Univ. of
Squibb, U.S.A.
Speaker/Author Poster Session

Pennsylvania, U.S.A.
Abstracts

B23 160
B15 152
Detection of Durvalumab-Induced Changes in
Evaluation of the Oxidant Status of Patients with
Activated and Proliferating T Cells in Melanoma
Rheumatoid Arthritis as a Potential Biomarker for
Patients Using Flow Cytometry-Based
Disease Progression
Immunophenotyping Assays
M. Chatterjee and S. De. Inst. of Postgrad. Med.
Index

Educ., Kolkata, India N. Standifer, J. Pearson, D. Sinibaldi and M. Liang.


MedImmune, CA and MD, U.S.A.

70 ISAC 2016 Program and Abstracts