FAKULTI TEKNOLOGI

KEJURUTERAAN
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FERMENTATION TECHNOLOGY (BNN 30304)

INDIVIDUAL ASSIGNMENT

QUESTION 1:
The air supply to a fermenter was turned off for a short period of time and then
restarted. A value for C* of 7.3 mg/l has been determined for the operating
conditions. Use the tabulated measurements of dissolved oxygen (DO) values to
estimate the oxygen uptake rate and kLa in this system

Time (min) DO (mg/ml)

-1 3.3
Air off 0 3.3
1 2.4
2 1.3
3 0.3
4 0.1
5 0.0
Air on 6 0.0
7 0.3
8 1.0
9 1.6
10 2.0
11 2.4
12 2.7
13 2.9
14 3.0
15 3.1
16 3.2
17 3.2

# use graph paper to plot the graph

QUESTION 2:
In cultivation of bakers yeasts in a stirred tank and aerated tank, lethal agents are
added to the fermentation medium to kill the organism immediately. Increase in
dissolved oxygen concentration upon addition of lethal agents is followed by the aid
of dissolved oxygen (DO) analyzer and a recorder. Using the following data in Table
1, determine the oxygen transfer coefficient (k La) for the bioreactor. Saturation DO
concentration is C* = 10 mg/l
Table 1: Tabulated measurement of dissolved oxygen

Time CL or DO
(minute) (mg/l)
1 1.5
2 3.2
2.5 4
3 5.5
4 6.5
5 7.2

# use graph paper to plot the graph

QUESTION 3:

Consider the scale up of a fermentation from 5L to 5000L vessel. The small

fermenter has a height-to diameter ratio of 3. The impeller diameter is 30% of the
tank diameter. Agitator speed is 700 rpm and three Rushton impellers are used.
Assume that fermenter is cylindrical.

(i) Determine the dimensions of the large fermenter

(ii) Calculate agitator speed at constant impeller tip speed

(iii) Calculate agitator speed at constant Reynolds number

(iv) Predict your conclusion from the results obtained in Q2(c)(ii) and
Q2(c)(ii)
 FORMULA:

2
1. V = r H
2
ND
2. =

P
N=
3. 3 5
N D

2 3
4. P=k 1 N D

3 5
5. P=N P N D

6. OTR = kLa (C*-CL)

7. OUR = qo2X