Medicina generala UMF Carol Davila

PROTOZOARE

Asistent universitar NEAGOE IONELA

AMIBE
AMIBE PATOGENE
Entamoeba hystolitica
 Entamoeba hystolitica
Stadiul de CHIST

Examen coproparazitologic = Frotiu din fecale coloratie Tricrom

 Entamoeba hystolitica
Trophe (gr)= Stadiul de TROFOZOIT
hranire; zoon
(gr)= animal

Examen
coproparazitologic =
Frotiu din fecale
muco-pio-
sangvinolente
coloratie Giemsa
 Entamoeba hystolitica
Colonoscopie Abcese primare in buton de camasa
 Entamoeba hystolitica
Abces primar in buton de camasa

Examen histopatologic = Sectiune tesut intestinal coloratie H&E

Entamoeba hystolitica
Abces secundar amoebian - hepatic
Entamoeba hystolitica
Abces secundar amoebian -perianal
AMIBE COMENSALE
 Entamoeba coli
Stadiul de CHIST

Examen 8 nuclei in
coproparazitologic planuri
= Preparat proaspat suprapuse
L+l din fecale +
solutie Lugol
AMIBE LIBERE CONDITIONAT
PATOGENE

1. Naegleria fowlery
2. Acanthamoeba sp.
Naegleria fowlery
 Naegleria fowlery

Stadiul de CHIST
 Naegleria fowlery
Stadiul FLAGELAT

Flageli
(2)
 Naegleria fowlery
Stadiul de TROFOZOIT

Recunoastere dupa nucleul cu aspect de tinta Examen histopatologic =

Sectiune tesut cerebral
coloratie H&E
Acanthamoeba sp.
 Acanthamoeba sp.
Stadiul de CHIST

Preparat
proaspat
L+l din
suspensie
tesut de
raclaj
corneean
 Acanthamoeba sp.
Stadiul de TROFOZOIT

Nucleu

Preparat
proaspat
L+l din
LCR Acantopodii
Acanthamoeba sp.
Keratita amoebiana
Acanthamoeba sp.
Abcese subcutanate granulomatoase (la
imunosupresati)
Acanthamoeba sp.
Encefalita granulomatoasa amoebiana
cronica (la imunosupresati)
FLAGELATE
FLAGELATE CAVITARE

1. Giardia duodenalis
2. Trichomonas vaginalis
Giardia duodenalis
 Giardia duodenalis

Stadiul de CHIST

Examen
coproparazitologic
= Preparat proaspat
L+l din fecale formate
+ Solutie Lugol
 Giardia duodenalis
Stadiul de TROFOZOIT

Examen
coproparazitolo
gic =
Frotiu fecale
diareice
coloratie Giemsa
 Giardia duodenalis
Stadiul de TROFOZOIT

Ventuza/Disc adeziv
Instalare MALABSORTII
Trichomonas vaginalis
Trichomonas vaginalis
Stadiul de TROFOZOIT

Frotiu secretie coloratie Giemsa

Trichomonas vaginalis
Coposcopie Vaginita + Leucoree FAZA INCIPIENTA
 Trichomonas vaginalis
Colposcopie Strawberry cervix + coinfectie cu Candida
 Trichomonas vaginalis

Testul InPouch TV pentru

detectia directa si cultivarea
trofozoitilor
 Trichomonas vaginalis
Test rapid imunocromatografic detectie
antigene
HEMOFLAGELATE

1. Leishmania sp.

2. Trypanosoma brucei
3. Trypanosoma cruzii
Leishmania sp.
 Leishmania sp.
(Sandfly)
Phlebotomus sp Lutzomia sp.
 Leishmania sp.
Stadiul PROMASTOGOT

Mastigot = Flagel liber

kinetida (gr) =
flagel+
corpuscul
bazal/kinetosom

kinetoplast

Frotiu GLANDE nucleu

SALIVARE
MUSCULITA DE
NISIP - coloratie
GIEMSA
 Leishmania sp.
Stadiul AMASTOGOT

Amastigot uninucleat

kinetoplast Nucleu
macrofag
Frotiu aspirat
medular
coloratie
Giemsa

Macrofag
Leishmania sp.
Buton de Orient
- Forma uscata - L. tropica
 Leishmania sp.
Buton Orient - Forma umeda L. major
 Leishmania sp.
Forma ESPUNDIA L. brasiliensis
Leishmania sp.
Ulcerul chiclero- L. mexicana
 Leishmania sp.
L. donovani
L. Infantum
L. chagasi
Boala Kala-
azar, febra
Dum-Dum,
boala
neagra
FAZA
CRONICA
Leishmanioza cutanata
post kala-azar FAZA
CRONICA

Leishmania sp.
Leismania donovani
Trypanosoma brucei
Trypanosoma brucei
Glossina sp. ( musca tze-tze )
 Trypanosoma brucei
Stadiul EPIMASTOGOT

Membrana ondulanta Flagel

scurta liber
anterior
Frotiu din
cultura
dezvoltata
pe mediul
NNN -
coloratie Nucleu
GIEMSA
 Trypanosoma brucei
Stadiul TRIPOMASTIGOT

Membrana
ondulanta lunga

Corpuscul bazal /Kinetosom

Examen
hematologic = Kinetoplast
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA Nucleu

Flagel liber
anterior
Trypanosoma brucei
gambiense
Sleeping sickness (Boala somnului)
FAZA INCIPIENTA

Semnul Winterbottom
 Trypanosoma brucei
Sleeping sickness (Boala
somnului) FAZA CRONICA

Meningoencefalita cronica
Trypanosoma cruzii
 Trypanosoma cruzii

Triatoma sp. (Kissing bug)

Reduviid bug
 Trypanosoma cruzii
Stadiul TRIPOMASTIGOT

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
Trypanosoma cruzii
Stadiul AMASTOGOT

Amastogoti
uninucleati

Celula
nervoasa

Aprenta tesut cardiac

coloratie Giemsa
Trypanosoma cruzii
Boala Chagasi (FAZA INCIPIENTA)

Semnul Romana
 Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Cardiomiopatie cu
megalie cardiaca

Anevrism
cardiac
Trypanosoma cruzii
Boala Chagasi (FAZA CRONICA)

Megacolon
SPOROZOARE
 Sporozoare tisulare

1. Toxoplasma gondii
Toxoplasma gondii
 Toxoplasma gondii
Stadiul de OOCHIST

P.C.

Sporozoiti
uninucleati

Sporochisti

Examen coproparazitologic fecale PISICA

 Toxoplasma gondii
Stadiul de TACHIZOIT (trofozoitul de Toxoplasma)

TACHIZOITI uninucleati
Frotiu
ascita
soarece
infectat
coloratie
Giemsa

Macrofag
tachys (gr)=rapid; zoon (gr)= animal
 Toxoplasma gondii
Stadiul de CHIST TISULAR cu bradizoiti

Celula
nervoasa

BRADIZOITI

Amprenta
tesut
cerebral
coloratie
bradys (gr)=lent; zoon (gr)= animal Giemsa
 Toxoplasma gondii
Hidrocefalie
 Toxoplasma gondii

Corioretinita
(la imunosupresati si la
nou-nascuti cu
toxoplasmoza
congenitala)
 Toxoplasma gondii
Neurotoxoplasmoza (la imunosupresati)

CT
 Sporozoare tisulare

2. Plasmodium vivax
Plasmodium ovale
Plasmodium malariae
Plasmodium falciparum
Plasmodium sp.
 Plasmodium sp.
Tantarul Anopheles sp.
Plasmodium sp.
OOCHISTI fixati pe fata
externa a stomacului unui
tantar infectat.
 Plasmodium sp.
SPOROZOITI

Frotiu GLANDE SALIVARE TANTAR - coloratie GIEMSA

 Plasmodium sp.
Amprenta
tesut
hepatic
coloratie
Giemsa

MEROZOITI INTRAHEPATOCITARI
 Plasmodium sp.
Plasmodium vivax

INEL= TROFOZOIT TANAR

Nucleu
inel Vacuola
centrala
Citoplasma
putina,
periferica
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
 Plasmodium sp.
Plasmodium vivax
AMOEBA = TROFOZOIT MATUR

Examen
hematologic =
Frotiu SANGE Citoplasma
PERIFERIC bogata, cu
coloratie multe
GIEMSA prelungiri Nucleu
amoeba
 Plasmodium sp.
Plasmodium vivax
ROZETA = SCHIZONT MATUR
16-24 merozoiti
Granulatii
Schuffner
Examen
hematologic =
Frotiu SANGE Nuclei
PERIFERIC merozoiti
coloratie
GIEMSA

Corp muriform
 Plasmodium sp.

Plasmodium vivax
GAMETOCIT MASCULIN
Examen Granulatii
hematologic = Schuffner
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA

Nucleu
central
nedefinit
 Plasmodium sp.
Plasmodium vivax
GAMETOCIT FEMININ
Granulatii
Schuffner

Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Nucleu gametocit -
periferic, triunghiular
 Plasmodium sp.
Plasmodium ovale
INEL= TROFOZOIT TANAR

Nucleu inel

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
 Plasmodium sp.
Plasmodium ovale
AMOEBA = TROFOZOIT MATUR

Examen
hematologic =
Frotiu SANGE
PERIFERIC Nucleu
coloratie amoeba
GIEMSA
 Plasmodium sp.
Plasmodium ovale
ROZETA = SCHIZONT MATUR
8-12 merozoiti

Frotiu
SANGE
PERIFERIC
coloratie
GIEMSA
Nuclei
merozoiti
 Plasmodium sp.
Plasmodium ovale
GAMETOCIT MASCULIN

Examen
hematologic =
Frotiu SANGE
PERIFERIC Granulatii
coloratie Schuffner /
GIEMSA James
 Plasmodium sp.
Plasmodium ovale
GAMETOCIT FEMININ
Granulatii
Schuffner /
James
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie Nucleu gametocit -
GIEMSA periferic, triunghiular
 Plasmodium sp.

Plasmodium malarie
INEL= TROFOZOIT TANAR

Examen
hematologic =
Frotiu SANGE
PERIFERIC Nucleu
coloratie inel
GIEMSA
 Plasmodium sp.

Plasmodium malarie
AMOEBA = TROFOZOIT MATUR

Amoeba in banda

Nucleu
Granulatii amoeba
Ziemann
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
 Plasmodium sp.
Plasmodium malarie
ROZETA = SCHIZONT MATUR
8-12 merozoiti
Rozeta in margareta

Granulatii
Ziemann

Nuclei
merozoiti Nuclei
granulocit
Examen hematologic = Frotiu SANGE Examen hematologic = Picatura groasa
PERIFERIC coloratie GIEMSA SANGE PERIFERIC coloratie GIEMSA
 Plasmodium sp.

Plasmodium malarie
GAMETOCIT MASCULIN
Granulatii
Ziemann
Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
 Plasmodium sp.

Plasmodium malarie
Granulatii GAMETOCIT FEMININ
Ziemann

Examen Nucleu gametocit -

hematologic = periferic, triunghiular
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
 Plasmodium sp.
Plasmodium falciparum
INEL= TROFOZOIT TANAR

Examen
hematologic =
Frotiu SANGE
PERIFERIC Hematie
coloratie multiparazitata
GIEMSA cu inele

Inele uni si bi nucleate

FAZA INCIPIENTA
 Plasmodium sp.

Plasmodium falciparum
AMOEBA = TROFOZOIT MATUR

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie GIEMSA

FAZA COMATOASA
 Plasmodium sp.

Plasmodium falciparum
ROZETA = SCHIZONT MATUR
8-32 merozoiti
Merozoiti
uninucleati

Examen
hematologic =
Frotiu SANGE
granulatii
PERIFERIC
Maurer
coloratie
GIEMSA
FAZA COMATOASA
 Plasmodium sp.
Plasmodium falciparum
GAMETOCIT MASCULIN in cosulet

Examen
hematologic = Nucleu granulatii
Frotiu SANGE gametocit Maurer
PERIFERIC
coloratie
GIEMSA

FAZA INCIPIENTA
 Plasmodium sp.
Plasmodium falciparum
GAMETOCIT FEMININ in cosulet

Examen
hematologic =
Frotiu SANGE
PERIFERIC
coloratie
GIEMSA
FAZA INCIPIENTA
 Plasmodium sp.

Plasmodium falciparum GAMETOCIT

MASCULIN

INEL

Examen
hematologic =
Frotiu SANGE GAMETOCITI FEMININI
PERIFERIC
coloratie
GIEMSA

FAZA INCIPIENTA
 Plasmodium falciparum
Neuropaludism Malarie cerebrala

Pete hemoragice
(petesii in rozeta)
= semn specific in
malaria cerebrala
 Teste imunocromatografice
pentru detectia antigenelor
- +
malarice

Test rapid bazat pe Test rapid bazat pe detectie dubla Ag

detectie Ag HRP2 de P.f pLDH de P.f si P,v
 Sporozoare digestive
3. Cryptosporidium parvum
Cryptosporidium hominis
Cryptosporidium sp.
 Cryptosporidium sp.
Stadiul de OOCHIST

Sporozoiti
uninucleati
(4)

Perete celular

Examen coproparazitologic = Frotiu fecale coloratie ZIEHL-NIELSEN modificata

Sporozoar digestiv

4. Isospora belli
 Isospora belli

immature
 Isospora belli
Stadiul de OOCHIST
Examen coproparazitologic

Oochist Oochist
matur (8) Sporozoiti imatur
uninucleati

(2)
Sporochisti

Frotiu fecale coloratie

Preparat
ZIEHL-NIELSEN
proaspat L+ l
modificata
din fecale cu SF
 Sporozoar - Transfug
5. Pneumocystis carinii
Pneumocystis jiroveci
 Pneumocystis carinii
Pneumocystis jiroveci
Cale
aerogena
 Pneumocystis carinii

TROFOZOITI (nuclei trofozoiti in nor cu puncte)

Frotiu LBA coloratie GIEMSA.

 Pneumocystis carinii

TROFOZOITI

CHISTI
cu (8)
corpusculii
colorati

Frotiu LBA coloratie GIEMSA.

 Pneumocystis carinii
CHISTI cu perete
celular colorat

Frotiu LBA coloratie Albastru de Toluidina.

Pneumocystis carinii

RX- plamani in geam mat

Pneumocitoza P.c interstitiala plasmocitara