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Gas Chromatography
Authors:
Nathan Kuffel
Timothy Ruppel
PerkinElmer, Inc.
Shelton, CT
2
Acetaldehyde
E thyl acetate
Acetone
Methanol
E thanol
N-propanol
T-butanol
0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40
Minutes
Figure 1. Chromatogram showing the elution order of the BAC mix that identified t-butanol as a suitable internal standard for the ethanol analysis.
1.40
1.20
1.00
0.80
Area Ratio
0.60
0.40
0.20
0.00
-0.20
0.00 50.00 100.00 150.00 200.00 250.00 300.00 350.00 400.00 450.00 500.00
Amount
Figure 2. Calibration curve of ethanol used for the analysis of fruit juice.
3
Conclusion For Further Reading:
This simplified method allows for favorable results in the 1. Timothy D. Ruppel; PerkinElmer Field Application Report,
quantification of ethanol content in consumable fruit juices. Blood Alcohol Analysis Utilizing Headspace Autosampling
Additionally, the headspace introduction of the sample to the GC and Dual-column GC Confirmation
ensures that the amount of sample that gets on to the column is 2. Timothy D. Ruppel; PerkinElmer Field Application Report,
consistent. By decreasing the chance of errors in the preparation Simulaneous and Rapid Separation of Blood-Alcohol
leads to concrete results that can be used as valid proof. These Compounds and Commonly Abused Inhalants by Headspace-
results are obtained from a TurboMatrix HS autosampler and the Gas Chromatography
necessary time taken in sample preparation of each individual run
due to the sensitivity of the headspace. 3. Paul L. Davis; Florida State horticultural Society, 1971,
Pg 217- 222
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