RAW DATA PNP 30M

0 0.1 0.2 0.3 0.4 0.5 0.6

m Mm mM mM mM mM mM
M
0 0 0.00 0.00 - - 0.01 -
4 7 0.00 0.00 9 0.01
8 3 2
30 0 0.02 0.05 - 0.11 0.14 0.06
9 0.00 5 1 4
4
60 0 0.05 0.09 - 0.16 0.22 0.14
3 4 0.00 7 1 1
1
90 0 0.08 0.14 0.00 0.24 0.35 0.26
7 3 5 9 9 1
12 0 0.11 0.18 0.00 0.29 0.41 0.34
0 5 9 9 5 6 6
15 0 0.15 0.23 0.01 0.34 0.49 0.45
0 8 5 8 8
18 0 0.17 0.28 0.02 0.42 0.56 0.53
0 6 7 5 8 9
21 0 0.21 0.33 0.02 0.51 0.68 0.65
0 5 7 5 2 3 5
24 0 0.24 0.38 0.03 0.56 0.75 0.73
0 3 4 3 3 4
27 0 0.28 0.43 0.03 0.60 0.84 0.83 CORRECTED DATA
0 3 9 9 30M5
PNP 1
30 0 0.31 0.48 0.04 0.64 0 0.91
0.1 0.92
0.2 0.3 0.4 0.5 0.6
0 7 5 5 m 9mM 2mM mM mM mM Mm
33 0 0.34 0.52 0.05 0.70 M 1.00 1.02
0 9 9 2 0 8 0 70 50 0 0 0 0
36 0 0.37 0.57 0.05 300.78
0 1.07
0.02 1.09
0.04 0.00 0.11 0.12 0.07
0 2 7 6 35 93 4 8 2 6
39 0 0.40 0.62 0.06 600.86
0 1.14
0.04 1.18
0.08 0.00 0.17 0.20 0.15
0 7 1 2 2 89 57 7 2 3
42 0 0.43 0.66 0.07 900.94
0 1.20
0.08 1.26
0.13 0.01 0.25 0.34 0.27
0 5 2 83 6 3 2 3
12 0 0.11 0.18 0.01 0.29 0.39 0.35
0 1 2 7 8 7 8
15 0 0.14 0.23 0.02 0.35 0.47 0.46
0 6 1 3 1 9 2
18 0 0.17 0.28 0.02 0.42 0.54 0.55
0 2 8 8 9 1
21 0 0.21 0.33 0.03 0.51 0.66 0.66
0 1 3 5 4 7
24 0 0.23 0.37 0.04 0.56 0.73 0.74
0 9 7 1 6 1 6
27 0 0.27 0.42 0.04 0.61 0.82 0.84
0 6 6 7 2 6 3
30 0 0.30 0.48 0.05 0.64 0.9 0.93
0 6 3 8 4
33 0 0.34 0.52 0.06 0.71 0.98 1.03
0 5 2 1 8 7
36 0 0.36 0.57 0.06 0.78 1.05 1.11
0 8 4 3 4 1
39 0 0.40 0.61 0.07 0.86 1.12 1.19
0 3 4 5 9 7
42 0 0.42 0.65 0.07 0.94 1.18 1.27
0 6 8 8 5 9 2
 M-M curve of PNP 30M
0.04

0.03

V 0.02
0.01

0
0 0.1 0.2 0.3 0.4 0.5 0.6
[S]

M-M curve PNP 10 M

0.05

V
0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
[S]

PNP 10 M (RAW
DATA)
0 0.1 0.2 0.3 0.4 0.5 0.6
mM mM mM mM mM mM mM
0 0.01 0.02 0.03 0.02 0.03 0.03 0.03
4 7 8 6 5 5 4
30 0.01 0.16 0.17 0.26 0.26 0.27 0.31
2 9 6 5 2 9
60 0.01 0.37 0.33 0.47 0.50 0.42 0.45
1 9 6 6 9 6
90 0.01 0.44 0.44 0.66 0.63 0.65 0.77
1 1 6 5 7 5 7
12 0.01 0.55 0.61 0.84 0.83 0.80 0.93
0 1 2 4 9 1 3 1
15 0.01 0.66 0.73 1.01 0.98 1.10 1.06
0 1 2 1 1 2 3 5
18 0.01 0.74 0.87 1.15 1.13 1.15 1.17
0 1 4 8 2 7 2
21 0.01 0.82 0.95 1.24 1.26 1.30 1.27
0 1 4 7 9 3 8 5
24 0.01 0.87 1.06 1.34 1.35 1.38 1.34
0 1 3 8 6 6 9
27 0.01 0.91 1.14 1.43 1.44 1.46 1.38
0 1 5 4 4 5 7 9
30 0.01 0.94 1.22 1.46 1.49 1.51 1.42 PNP 10M
0 1 7 4 7 8 5 [S Vmax [1/S] 1/Vmax
33 0.01 0.98 1.27 1.50 1.54 1.56 1.46
]
0 1 9 6 5 2
0. 0.03608 10 27.7112
36 0.01 1.01 1.32 1.54 1.58 1.6 1.47
0 1 7 7 5 5 1 6 5
39 0.01 1.03 1.37 1.55 1.60 1.61 1.49 0. 0.03857 5 25.9267
0 1 9 2 4 6 1 2 2
42 0.01 1.07 1.41 1.57 1.62 1.62 1.50 0. 0.03958 3.33333 25.2653
0 1 4 4 4 7 3 3 3 1
45 0.01 1.10 1.43 1.58 1.63 1.64 1.51 0. 0.03768 1.66666 26.5380
0 1 1 6 4 3 3 4 6 2 7 5

PNP 10 M
(CORRECTED DATA)
0 0.1 0.2 0.3 0.4 0.5 0.6
m m m m m m m
M M M M M M M
0 0 0 0 0 0 0 0
3 0 0.1 0.1 0.2 0.2 0.2 0.2
0 35 41 4 3 37 85
6 0 0.3 0.2 0.4 0.4 0.3 0.4
0 52 92 5 71 94 22
9 0 0.4 0.4 0.6 0.6 0.6 0.7
0 14 08 39 02 2 43
1 0 0.5 0.5 0.8 0.7 0.7 0.8
2 25 76 23 96 68 97
0
1 0 0.6 0.6 0.9 0.9 1.0 1.0
5 35 93 85 47 68 31
0
1 0 0.7 0.8 1.1 1.0 1.1 1.1
8 17 4 24 97 22 38
0
2 0 0.7 0.9 1.2 1.2 1.2 1.2
1 97 19 23 28 73 41
0
2 0 0.8 1.0 1.3 1.3 1.3 1.3
4 46 3 2 21 54 06
0
2 0 0.8 1.1 1.4 1.4 1.4 1.3
7 88 06 08 1 32 55
0
3 0 0.9 1.1 1.4 1.4 1.4 1.3
 0 2 82 38 62 83 91
0
3 0 0.9 1.2 1.4 1.5 1.5 1.4
3 62 38 79 05 27 26
0
3 0 0.9 1.2 1.5 1.5 1.5 1.4
6 9 89 19 45 65 41
0
3 0 1.0 1.3 1.5 1.5 1.5 1.4
9 12 34 28 71 76 58
0
4 0 1.0 1.3 1.5 1.5 1.5 1.4
2 47 72 48 89 92 69
0
4 0 1.0 1.3 1.5 1.5 1.6 1.4
5 74 98 58 98 08 8
0

PNP 30M
[S V 1/ 1/V LB plot PNP 10M
] [S]
0. 0.03176 10 31.4812 28
1 5 5 f(x) = 0.36x + 24.07
27 R = 1
0. 0.03418 5 29.2498
2 8 5
0. 0.03493 2.5 28.6242 1/V 26
4 5 8 25
0. 0.03634 2 27.5111
5 9 1 24
2 3 4 5 6 7 8 9 10 11
1/S

PNP without inhibitor( corrected

data)
0 0. 0. 0. 0. 0. 0.
m 1 2 3 4 5 6
M m m m m m m
M M M M M M
3 0 0. 0. 0. 0. 0. 0.
0 12 13 13 14 14 26
 2 4 5 4 7 2
6 0 0. 0. 0. 0. 0. 0.
0 24 27 28 30 31 54
3 5 2 3 7 2
9 0 0. 0. 0. 0. 0. 0.
0 35 40 42 46 49 79
7 3 7 4 9
1 0 0. 0. 0. 0. 0. 0.
2 46 52 56 61 67 99
0 7 6 3 4
1 0 0. 0. 0. 0. 0. 1.
5 55 64 7 75 83 14
0 6 9 7 5 1
1 0 0. 0. 0. 0. 0. 1.
8 64 75 82 88 97 23
0 3 6 1 7 5 2
2 0 0. 0. 0. 0. 1. 1.
1 72 85 93 99 08 28
0 1 6 7 5 3
2 0 0. 0. 1. 1. 1. 1.
4 79 94 02 09 17 31
0 1 2 3 2
2 0 0. 1. 1. 1. 1. 1.
7 85 02 10 16 23 32
0 2 3 3 9 4 8
3 0 0. 1. 1. 1. 1. 1.
0 90 08 16 22 28 33
0 5 6 6 3 3 6
3 0 0. 1. 1. 1. 1. 1.
3 95 14 21 26 31 34
0 2 3 7 6 4 1
3 0 0. 1. 1. 1. 1. 1.
6 99 18 25 29 33 34
0 3 9 8 8 6 4
3 0 1. 1. 1. 1. 1. 1.
9 02 22 28 31 28 34
0 7 6 9 8 6
4 0 1. 1. 1. 1. 1. 1.
2 05 25 31 33 36 34
0 8 6 5 1 8
4 0 1. 1. 1. 1. 1. 1.
5 08 28 32 34 36 34
0 5 9 8 3 8

RAW DATA (WITHOUT INHIBITOR)

0 0. 0. 0. 0. 0. 0.
m 1 2 3 4 5 6
M m M m M M M
M m M m m m
0 0 0.0 0.0 0.0 0.0 0.0 0.0
22 23 31 3 39 56
3 0 0.1 0.1 0.1 0.1 0.1 0.3
0 44 57 66 74 86 18
6 0 0.2 0.2 0.3 0.3 0.3 0.5
0 65 98 13 33 56 98
9 0 0.3 0.4 0.4 0.4 0.5 0.8
0 79 26 58 9 33 55
1 0 0.4 0.5 0.5 0.6 0.7 1.0
2 82 5 97 43 09 5
0
1 0 0.5 0.6 0.7 0.7 0.8 1.1
5 78 72 31 87 74 97
0
1 0 0.6 0.7 0.8 0.9 1.0 1.2
8 65 79 52 17 14 88
0
2 0 0.7 0.8 0.9 1.0 1.1 1.3
1 43 79 61 27 24 39
0
2 0 0.8 0.9 1.0 1.1 1.2 1.3
4 13 65 54 2 09 68
0
2 0 0.8 1.0 1.1 1.1 1.2 1.3
7 74 46 34 99 73 84
0
3 0 0.9 1.1 1.1 1.2 1.3 1.3
0 27 09 97 53 22 92
0
3 0 0.9 1.1 1.2 1.2 1.3 1.3
3 74 66 48 96 53 97
0
3 0 1.0 1.2 1.2 1.3 1.3 1.4
6 15 12 89 28 75
0
3 0 1.0 1.2 1.3 1.3 1.3 1.4
9 49 49 2 48 19 02
0
4 0 1.0 1.2 1.3 1.3 1.4 1.4
2 8 79 41 65 04
0
4 0 1.1 1.3 1.3 1.3 1.4 1.4
5 07 03 6 78 02 04
0

LB PLOT PNP Without inhibitor

70

1/V 20 f(x) = 0.26x + 24.68

R = 0.83
0 50
-30
1/[S]
MSP without inhibitor
0m 0.1 0.2 0.3 0.4 0.5 0.6
M mM mM mM mM mM mM
0 0 0.07 0.04 0.07 0.06 0.06 0.05
9 2 3 2 3
3 0 0.31 0.38 0.51 0.43 0.33 0.68
0 7 5 4 1 6 8
6 0 0.47 0.68 0.75 0.76 0.57 0.95
0 1 9 7 7 3 5
9 0 0.60 0.90 1.01 1.16 0.86 1.14
0 9 6 6 6 7 9
1 0 0.71 1.09 1.35 1.35 1.05 1.36
2 4 8 2 2 9 6
0
1 0 0.80 1.23 1.49 1.51 1.30 1.52
5 8 4 5 5 5 3
0
1 0 0.88 1.34 1.57 1.57 1.41 1.59
8 9 5 5 3 4 6
0
2 0 0.95 1.43 1.62 1.63 1.53 1.64
1 3 9 5 2 8 2
0
2 0 1.01 1.49 1.62 1.65 1.58 1.65
4 2 5 7 7 5 9
0
2 0 1.06 1.53 1.67 1.67 1.62 1.67
7 2 2 4 4 9 6
0
3 0 1.10 1.56 1.68 1.68 1.64 1.68
0 2 6 2 3 5 4
0
3 0 1.13 1.58 1.69 1.68 1.66 1.69
3 6 7 1 3 1 3
0
3 0 1.16 1.61 1.7 1.68 1.67 1.69
6 9 3 3
0
3 0 1.19 1.61 1.7 1.68 1.67 1.69
9 5 8 3 8 3
0
4 0 1.21 1.62 1.7 1.68 1.68 1.69
2 8 6 3 7 3
0
4 0 1.21 1.63 1.7 1.68 1.68 1.69
5 8 4 3 7 3
0

CORRECTED DATA
0mM 0.1mM 0.2mM 0.3mM 0.4mM 0.5mM 0.6mM
0 0 0 0 0 0 0 0
30 0 0.247 0.336 0.442 0.368 0.274 0.635
60 0 0.401 0.64 0.685 0.704 0.511 0.902
90 0 0.539 0.857 0.944 1.103 0.805 1.096
12 0 0.644 1.049 1.28 1.289 0.997 1.313
0
15 0 0.738 1.185 1.423 1.452 1.243 1.47
0
18 0 0.819 1.296 1.503 1.51 1.352 1.543
0
21 0 0.883 1.39 1.553 1.569 1.476 1.589
0
24 0 0.942 1.446 1.555 1.594 1.523 1.606
0
27 0 0.992 1.483 1.602 1.611 1.567 1.623
0
30 0 1.032 1.517 1.61 1.62 1.583 1.631
0
33 0 1.066 1.538 1.619 1.62 1.599 1.64
0
36 0 1.099 1.561 1.628 1.62 1.608 1.64
0
39 0 1.125 1.569 1.628 1.62 1.616 1.64
0
42 0 1.148 1.577 1.628 1.62 1.625 1.64
0
45 0 1.148 1.585 1.628 1.62 1.625 1.64
0

Without MSP
[S V 1/[S] 1/V
]
0. 0.03796 10 26.3404
1 4 3
0. 0.04604 3.33333 21.7193
3 2 3
0. 0.04846 2 20.6333
5 5 3
 RAW DATA
MSP
0 0.1 0.2 0.3 0.4 0.5 0.6
m mM Mm mM mM mM mM
M
0 0 0.0 0.0 0.0 0.0 0.0 0.0
05 26 29 23 23 24
3 0 0.1 0.1 0.1 0.1 0.1 0.2
0 44 59 73 56 74 12
6 0 0.3 0.3 0.3 0.3 0.3 0.4
0 43 16 75 26 39 04
0 0.4 0.4 0.5 0.5 0.4 0.6
9 47 7 01 74 1
0
1 0 0.5 0.5 0.6 0.6 0.6 0.7
2 34 99 59 63 42 78
0
1 0 0.6 0.7 0.8 0.8 0.7 0.9
5 19 36 36 28 86 69
0
1 0 0.7 0.8 0.9 0.9 0.9 1.1
8 82 38 72 43 28
0
2 0 0.7 0.9 1.0 1.1 1.0 1.2
1 63 87 58 02 78 53
0
2 0 0.8 1.0 1.1 1.2 1.1 1.3
4 22 67 66 19 95 53
0
2 0 0.8 1.1 1.2 1.3 1.2 1.4
7 71 5 45 22 89 34
0
3 0 0.9 1.2 1.3 1.4 1.3 1.5
0 61 26 23 03 7 02
0
3 0 0.9 1.2 1.3 1.4 1.4 1.5
3 59 88 86 74 38 47
0
3 0 0.9 1.3 1.4 1.5 1.4 1.5
6 92 41 35 26 88 75
0
3 0 1.0 1.3 1.4 1.5 1.5 1.6
9 27 91 82 65 31 05
0
4 0 1.0 1.4 1.5 1.6 1.5 1.6
2 56 24 18 64 21
0
4 0 1.0 1.4 1.5 1.6 1.5 1.6
5 83 6 4 23 93 37
0

CORRECTED DATA WITH MSP

0 0.1 0.2 0.3 0.4 0.5 0.6
m mM mM mM mM mM mM
M
0 0 0 0 0 0 0 0
30 0 0.13 0.13 0.14 0.13 0.15 0.18
9 3 4 3 1 8
60 0 0.33 0.29 0.34 0.30 0.31 0.38
8 6 3 6
90 0 0.44 0.44 0.47 0.47 0.45 0.58
2 4 2 7 1 6
12 0 0.52 0.57 0.63 0.64 0.61 0.75
0 9 3 9 4
15 0 0.61 0.71 0.80 0.80 0.76 0.94
0 4 7 5 3 5
18 0 0.69 0.85 0.90 0.94 0.92 1.10
0 5 6 9 9 4
21 0 0.75 0.96 1.02 1.07 1.05 1.22
0 8 1 9 9 5 9
24 0 0.81 1.04 1.13 1.19 1.17 1.32
0 7 1 7 6 2 9
27 0 0.86 1.12 1.21 1.29 1.26 1.41
0 6 4 6 9 6
30 0 0.95 1.2 1.29 1.38 1.34 1.47
0 6 4 7 8
33 0 0.95 1.26 1.35 1.45 1.41 1.52
0 4 2 7 1 5 3
36 0 0.98 1.31 1.40 1.50 1.46 1.55
0 7 5 6 3 5 1
39 0 1.02 1.36 1.45 1.54 1.50 1.58
0 2 5 3 2 8 1
42 0 1.05 1.39 1.48 1.57 1.54 1.59
0 1 8 9 7 1 7
45 0 1.07 1.43 1.51 1.6 1.57 1.61
0 8 4 1 3
ALKALINE PHOSPHATASE
[S V 1/[S] 1/V
]
0. 0.002607 10 383.5827
1
0. 0.00741 2 134.9528
5
1 0.0084 1 119.0476
2 0.0081 0.5 123.4568
500
400 0.01

300 f(x) = 28.78x + 93.12 0.01

R = 0.99
200 0.01
100 0
0 0
0 2 4 6 8 10 12
0
Linear ()
0 0.5 1 1.5 2 2.5

ALKALINE PHOSPHATASE REACTION RAW DATA

0 0.1 0.5 1 2 2.5 3
m Mm mM mM mM mM mM
M
0 0 0.02 0.05 0.06 0.04 0.06 0.02
4 3 4 6 2 9
30 0 0.12 0.38 0.29 0.28 0.32 0.19
1 4 7 2 6 7
60 0 0.25 0.57 0.78 0.74 0.82 0.82
9 1 2 9 8 2
90 0 0.33 0.79 1.57 1.46 1.51 1.46
6 9 2 1 6 3
12 0 0.42 1.01 2.52 2.38 2.39 2.23
0 6 6 6 6 7 8
15 0 0.49 1.21 3.67 3.48 3.49 3.27
0 4 8 2 1 5
18 0 0.58 1.34 4.93 4.72 4.65 4.46
0 6 9 2 4 2
21 0 0.65 1.45 6.30 6.08 5.92 5.75
0 5 5 6 7 8 6
24 0 0.73 1.53 7.72 7.53 7.29 7.11
0 7 2 6 3 2 3
27 0 0.78 1.58 9.18 9.02 8.72 8.55
0 9 9 7 2
30 0 0.86 1.61 10.6 10.5 10.2 9.98
0 8 8 51 09 8
33 0 0.91 1.64 12.1 12.0 11.7 11.4
0 6 2 85 99 24 82
36 0 0.96 1.65 13.7 13.6 13.2 12.9
0 7 63 61 81
39 0 1.00 1.65 15.2 15.2 14.8 14.4
0 5 9 2 36 14 8
42 0 1.06 1.66 16.7 16.8 16.3 15.9
0 8 45 09 75 89
45 0 1.09 1.66 18.2 18.3 17.9 17.5
0 8 8 76 91 52 04
48 0 1.14 1.67 19.8 19.9 19.5 19.0
0 7 12 82 29 19
51 0 1.16 1.67 21.3 21.5 21.1 20.5
0 5 7 48 73 14 34
54 0 1.19 1.68 22.8 23.1 22.6 22.0
0 4 6 89 64 99 49
57 0 1.27 1.68 24.4 24.7 24.2 23.5
0 6 3 55 84 64
60 0 1.27 1.68 25.9 26.3 25.8 25.0
0 6 71 46 69 79

ALKALINE PHOSPHATASE REACTION CALCULATED

DATA
0 0.1 0.5 1 2 2.5 3
mM Mm mM mM mM mM mM
30 0 0.09 0.33 0.23 0.23 0.26 0.16
7 1 3 6 4 8
60 0 0.23 0.51 0.48 0.46 0.50 0.62
5 8 5 7 2 5
90 0 0.31 0.74 0.79 0.71 0.68 0.64
2 6 2 8 1
12 0 0.40 0.96 0.95 0.92 0.88 0.77
0 2 3 4 5 1 5
15 0 0.47 1.16 1.14 1.09 1.09 1.03
0 5 4 6 4 7
18 0 0.56 1.29 1.26 1.24 1.16 1.18
0 2 6 2 2 1 5
21 0 0.63 1.40 1.37 1.36 1.27 1.29
0 1 2 4 3 6 6
24 0 0.71 1.47 1.42 1.44 1.36 1.35
0 3 9 6 4 7
27 0 0.76 1.52 1.46 1.49 1.43 1.43
0 5 7 3 4 7
30 0 0.83 1.56 1.49 1.52 1.48 1.43
0 6 5 1 4 7 8
33 0 0.89 1.58 1.50 1.54 1.51 1.49
0 2 9 5 8 5 4
36 0 0.94 1.59 1.51 1.56 1.53 1.49
0 3 7 5 4 7 9
39 0 0.98 1.60 1.52 1.57 1.55 1.49
0 1 6 3 3 9
42 0 1.03 1.61 1.52 1.57 1.56 1.50
0 6 5 5 3 1 9
45 0 1.07 1.61 1.53 1.58 1.57 1.51
0 4 5 1 2 7 5
48 0 1.11 1.62 1.53 1.59 1.57 1.51
0 6 4 6 1 7 5
51 0 1.14 1.62 1.53 1.59 1.58 1.51
0 1 4 6 1 5 5
54 0 1.17 1.63 1.54 1.59 1.58 1.51
0 3 1 1 5 5
57 0 1.24 1.63 1.54 1.59 1.58 1.51
0 6 3 1 1 5 5
60 0 1.24 1.63 1.54 1.59 1.58 1.51
0 6 3 1 1 5 5
 MSP 2mM
[S] V 1/[S] 1/V ABS vs TIME
0.1 0.03433 10 29.12941
AbOSBANCE AT 405 0
0.2 0.03790 5 26.38253 500
4 Time (secs)
0 1000
0.4 0.04038 2.5 24.76
8 0 0.1 0.5
0.6 0.04254 1.66666 23.50261 1 2 2.5
8 7
3