EFFECT OF PH ON

ENZYME REACTION

MOHAMAD AMIRUL AMIN BIN DAUD

D20162075576
MUHAMAD IKRAM BIN AHMAD NASIR
D20162075578
MUHAMMAD AFIQ BIN MOHD NOR
D20162075546
 INTRODUCTION

What Is Ph?

Measure Of The Hydrogen Ion Concentration Of A Solution.

What Is Enzyme Reaction?

An Uncatalyzed Reaction Requires A Higher Activation Energy Than Thus A Catalyzed

Reaction.

What Is Data Logger?

An Electronic Device That Records Data Over Time Or In Relation To Location Either With
A Built In Instrument Or Sensor Or Via External Instruments And Sensors.
 ENGAGE

At what pH is the rate of enzyme activity the highest?
The rate of enzyme activity which is the highest is at pH 10.
At what pH is the rate of enzyme activity the lowest?
The rate of enzyme activity which is the lowest at pH 4.
How does changing the pH affect the rate of enzyme activity?
Usually, the enzyme activity increases from pH 4 to 10. At low pH values, the protein may denature or
change its structure. This may affect the enzymes ability to recognize a substrate or it may alter its polarity
within a cell.
What is the function of the enzyme?
Enzymes are biological molecules (typically proteins) that significantly speed up the rate of virtually all of
the chemical reactions that take place within cells. They are vital for life and serve a wide range of
important functions in the body, such as aiding in digestion and metabolism.
 EMPOWER

1. Place three clean test tubes in a rack and label them pH 4, pH 7, and pH
10.

2. Add 5 mL of 3% H2O2 and 5 mL of a pH buffer to each test tube, as in

Table 1.

3. Using the test tube labeled pH 4, add 10 drops of enzyme solution.

4. Using the test tube labeled pH 7, add 10 drops of enzyme solution.

5. Using the test tube labeled pH 10, add 10 drops of enzyme solution.

6. Graph all three runs of data on a single graph.


 ENHANCE

FUNCTION
Each Enzyme Works Within Quite A Small Ph Range. There Is A Ph At Which Its
Activity Is Greatest (The Optimal Ph). This Is Because Changes In Ph Can Make And
Break Intra- And Intermolecular Bonds, Changing The Shape Of The Enzyme And,
Therefore, Its Effectiveness.

IMPORTANCE

Copper Enzymes In Isolated Chloroplasts. Polyphenoloxidase In Beta Vulgaris

Ultrasonic Processing On Food Enzyme

Influence Of Lime, Fertilizer And Manure Applications On Soil Organic Matter Content
And Soil Physical Conditions
 EXTENSION

1. Different organisms often live in very different habitats. Design a series
of experiments to investigate how different types of organisms might affect
the rate of enzyme activity. Consider testing a plant, an animal, and a protist.

2. Presumably, at higher concentrations of H2O2, there is a greater chance

that an enzyme molecule might collide with H2O2. If so, the concentration of
H2O2 might alter the rate of oxygen production. Design a series of
experiments to investigate how differing concentrations of the substrate
hydrogen peroxide might affect the rate of enzyme activity.
 CONCLUSION

The higher the pH level, the faster the enzymatic reaction until it reach
it optimum pH