S96 Abstracts / Current Opinion in Biotechnology 22S (2011) S15S152
of 2 months, in 2011. Ten samples from each store were collected
and transferred to the laboratory. For Salmonella isolation, con-
ventional culture methods were performed. For conrmation and
S. enteritidis serovar determination, a PCR reaction assay was used,
with Prot6E gene primers (Prot6e-5 and Prot6e-6), specic for S.
enteritidis serovar. 37 samples out of 100 showed positive results
for Salmonella spp. presence, while 24 out of them were contam-
inated with Salmonella enteritidis. The main conclusion was that
table eggs represent a potential risk considering salmonellosis,
especially due to the existence of S. enteritidis serovar.
doi:10.1016/j.copbio.2011.05.298
I23
Relationship of somatic cell count with milk yield and
composition in Holstein and Romanian Spotted cow
crossbreed population
Lucian Ionel Ilie 1 , Anca Maria Galis 2 , Laurentiu Tudor 1
1
Veterinary Medicine Department, University of Agricultural Sciences
and Veterinary Medicine of Bucharest, Romania
2 Animal Science Department, University of Agricultural Sciences and
Veterinary Medicine of Bucharest, Romania
E-mail address: drlucianilie@yahoo.com (L.I. Ilie)
The objective of this study was to analyze the relationship
between somatic cell count (SCC), milk yield (MY), fat and protein
percentage (FP/PP), fat and protein yield (FY/PY), using analysis of
variance and correlation analysis in Romanian crossbreed cows.
The records were obtained from ten commercial herds, located in
different counties near Bucharest, during September 2010 and Jan-
uary 2011. The cows presented different ages, being at rst, second,
third, fourth or later parity, this specic fact being used as a varia-
tion factor considering the studied parameters. MY, FP, PP, FY and
PY showed variations, with the highest values for cows at rst and
second parity, while the lowest values were associated with cows
at fourth and later parity, the situation for SCC being totally oppo-
site. Analysis of variance showed highly signicant effect of SCC
values on MY and composition. The correlations were highly neg-
ative between SCC, MY and PY, and highly positive for SCC, FY, FP
and PP. There was no signicant relationship between FP and PY
(P > 0.05). The results of the present study provide relevant data for
assessing milk production in an area considered important for milk
supply in Bucharest.
doi:10.1016/j.copbio.2011.05.299
I24
PCR detection of genetically modied maize and soya in
mildly and highly processed foods
Ozge Ozgen Arun, Funda Yilmaz
Besin Hijyeni ve Teknolojisi Anabilim Dali, Veteriner Fakultesi, Istan-
bul, Turkey
E-mail address: oarun@istanbul.edu.tr (O.O. Arun)
Despite the announced advances, genetically modied organ-
isms (GMO) have received a very strong consumer reaction since
they were rst commercialized. For this purpose numerous coun-
tries set up their ofcial regulations on labeling of GMOs and GMO
derivate foods. Finally regulation on traceability and labeling of GM
foods, have come into force in our country. The Regulation stip-
ulates the labeling of foods that contain GM material above the
threshold level. However, the current situation about the foods
we consume is pretty uncertain. In this present study detection
of GM maize and soya in food products obtained from local retail
shops was performed. The samples included, maize our, soya
our, canned maize, bread, cake, meat products and similar soya
and maize containing foods. The samples were analyzed for CaMV
35S promoter and nos terminator sequences which are the most
common sequences inserted in GM crops. The samples positive
for both or either sequences were evaluated as GM positive. The
negative maize and soya samples were detected for zein and lectin
respectively for the determination of detectible DNA in the sample.
The results indicated that 10 of 30 samples detected, were positive
for either or both of the novel sequences.
doi:10.1016/j.copbio.2011.05.300
I25
Encapsulation of food avors via coacervation method
Perihan Merve Buldur, Fatma Nese Kok
Molecular Biology and Genetics Department, Istanbul Technical Uni-
versity, Istanbul, Turkey
E-mail address: perihan.merve@gmail.com (P.M. Buldur)
Flavors are used in many areas, such as food, cosmetic, phar-
maceutical and detergent industries to increase taste and/or odor.
These highly valuable compounds lose their effect beginning from
the production step due to their volatile and oxidation labile
nature. The decreases in properties continue during the transfer
and storage of the product. Encapsulation of avors into differ-
ent materials (e.g. gelatin, gum arabic, whey proteins, and pectin)
is a well known method to solve this problem. In this study, the
potential of coacervation method in the efcient encapsulation
of food avors were studied. The model avors, d-limonene and
2-phenylethanol, were encapsulated into different ratio of gum
arabic/gelatin mixtures. Prepared microcapsules were crosslinked
by a natural, non-toxic agent, namely genipin. The microbeads were
then collected and dried by lyophilization. Preparation conditions
and different crosslinking agent concentrations were studied for
their effect on coacervation properties and avor retention dur-
ing drying and storage. Microbeads were visualized in uorescence
microscopy using nile red as lipid staining dye. It was seen that
increasing the gelatin and gum arabic concentration in the medium
to more than 40% and 50%, respectively, caused aggregation of the
newly formed microbeads. Optimum ratio was found to be 2:3 for
gum arabic/gelatin.
doi:10.1016/j.copbio.2011.05.301
I26
Selection of lactic acid bacteria strains producing
exopolysaccharides
Ionela Sarbu 1 , Tatiana Vassu 1 , Ileana Stoica 1 , Emanuel Vamanu 2 ,
Diana Roxana Pelinescu 1
1Genetics department, Faculty of Biology, Bucharest, Romania
2Biotechnology Department, Faculty of Biotechnology, University of
Agronomical Science and Veterinary Medicine, Bucharest, Romania
E-mail address: drpelinescu@yahoo.com (D.R. Pelinescu)
Production of exopolysaccharides (EPS) generated a high inter-
est due to their important industrial application and benecial
effects on human health. Main goal of our study was selection of