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  • Volume Fraction Measurement - ImageJ Instructions PDF

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    didap2002
    324 vizualizări2 pagini
    The document provides instructions for using ImageJ software to determine the volume fraction of phases in a micrograph through automatic thresholding and particle analysis. The key steps are to open the micrograph, adjust the threshold to isolate the phase of interest, run a particle analysis to determine the black/red pixel area fraction, and iterate the thresholding and analysis to get the most accurate measurement. Results from the final analysis should be saved, along with representative micrographs of the sample. Volume fractions are then added to a class dataset along with sample composition and analysis comments.

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    The document provides instructions for using ImageJ software to determine the volume fraction of phases in a micrograph through automatic thresholding and particle analysis. The key steps are to open the micrograph, adjust the threshold to isolate the phase of interest, run a particle analysis to determine the black/red pixel area fraction, and iterate the thresholding and analysis to get the most accurate measurement. Results from the final analysis should be saved, along with representative micrographs of the sample. Volume fractions are then added to a class dataset along with sample composition and analysis comments.

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    © All Rights Reserved
    Descărcați ca PDF, TXT sau citiți online pe Scribd
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    324 vizualizări2 pagini

    Volume Fraction Measurement - ImageJ Instructions PDF

    Încărcat de

    didap2002
    The document provides instructions for using ImageJ software to determine the volume fraction of phases in a micrograph through automatic thresholding and particle analysis. The key steps are to open the micrograph, adjust the threshold to isolate the phase of interest, run a particle analysis to determine the black/red pixel area fraction, and iterate the thresholding and analysis to get the most accurate measurement. Results from the final analysis should be saved, along with representative micrographs of the sample. Volume fractions are then added to a class dataset along with sample composition and analysis comments.

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    © All Rights Reserved
    Descărcați ca PDF, TXT sau citiți online pe Scribd
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    InstructionsforVolumeFractionMeasurementinImageJ:AutomaticThresholdMethod

    1. StarttheImageJsoftware.Usetheversioninstalledontheworkstationsinthelab.
    2. OpenthemicrographtobeanalyzedinImageJ
    3. Savetheimagetoanewnamesothatyoudonotaccidentallyoverwritetheoriginalimageas
    analyzeit.
    4. PrepareImageforParticleAnalysis
    o Iftheimagehasascalebar,cropittoexcludethescalebar.
    Highlightareatoretain
    ImageCrop
    Ifyousavethecroppedimage,makesureyoudidstep3first!
    o Converttheimagetoan8bitimage
    ImageType8bit
    o Savetheimage.Onelastchancehere:Makesureyoudontoverwritetheoriginalimage!
    o Adjustthethresholdforparticledetection.Usingthemenuslistedbelow,adjustthe
    thresholdparametersuntilthephaseforwhichyouwishtodeterminethevolumefraction
    isallred.Thegraybackgroundofyouroriginalimageshouldjustdisappearunderthered.
    ImageAdjustThreshold
    Usetheslidebarstocompletelycolorredthephaseofinterest,butnothingelse.This
    stepfrequentlyrequiressomejudgmentandcompromise.Itmaybehelpfultoopenthe
    originalimageinaseparatewindowtocomparetothethresholdedimageasyou
    manipulatethethresholdsendbars.
    PressApply.
    Theimageshouldturnblackandwhite.
    5. AnalyzeParticles/GrainSizeDetermination
    Thesoftwareisgoingtodeterminewhatfractionoftheareaofthisimageisblack.
    o Createaseparatefoldertosaveyourworkinatthispoint.Itmaybeprudenttosaveafew
    examplesoftheanalysistoreferbacktoasyouattempttomakethebestmeasurementof
    thevolumefractionofeachphaseinthemicrostructureyoucan.
    o Runtheanalysis
    AnalyzeAnalyzeParticles
    Forthefirstattempt,setsizeas0infinity.Thiswillcounteachred/blackpixelas
    apartofthephaseyouaretryingtomeasure.Selectingalargerminimum
    resultsintheprogramincludingaparticleintheareafractionmeasurement
    onlyifitscontiguousareaisgreaterthanthespecifiednumberofpixels
    squared.
    SelectShowOutlinesDisplayResults,ClearResults,andSummarize.
    ThenclickOK

    Theoutputwillappearitthreenewwindows.Thefirstisanimageinawindow
    calleddrawingoffilename.Thesecondisatextboxthatcontainsnumerical
    results.ThethirdisasecondtextboxcalledSummary..Studywhatisineach
    windowcarefullytoseewhatinformationisprovidedbeforeyoucontinue.

    Iterateonthestepsabovetodetermineavolumefractionmeasurementyouare
    confidentin.
    Runningnewanalyzeswillresultinthesoftwarereplacingtheimageinthe
    drawingwindoweachtime,sobesuretosaveanyimagesyouwanttosave.The
    newresultsareaddedtothebottomofthetextwindoweachtimeananalysisis
    run.Besuretodocumentinyournotebookwhatthesettingswerethatresulted
    ineachmeasurementthatappearsinthetextwindowandhowconfidentyou
    areinthatparticularanalysis.
    Besuretosaveacopyofthedrawingofthetestresultsfromthefinalanalysis
    youacceptasbeingasgoodasyoucanget.
    Ifthesampleappearstocontainaprimaryphase,aprecipitatedphase,oraeutectic
    component,usethemethodsabovetoestimatethevolumefractionoftheprimary
    phaseorprecipitatedphase.Onewaythatmightworkistofiddlewiththeparticlesize
    definitionstogetthesoftwaretocountonlyverylargeorverysmallparticles..
    Theresultswindowrecordsallofthedatapointsusedtodeterminethevolumefraction
    ofthephase.Thesummarywindowrecordstheareafractionofthephaseofinterestfor
    each.
    Savetheresults/summarydatayouwanttokeep.
    CheckSetFileExtensionandsetitto.xlstosavetoanexcelfileyoucanwork
    withlater.
    6. Enterthesamplecomposition,theresultsofyourquantitativeanalysisofthemicrostructure,
    andanycommentsyouwanttomakeintheclassdataset.Ifyouaredonefirst,pleasesetupa
    spreadsheetfortheclassdata.
    7. Pickoneortwoimagesthatyouthinkshowthemicrostructureofthesampleparticularlywell
    andaddthemtothefinalimagesfolder.Makesurethealloycompositionisnotedinthefile
    name.

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