CURRICULUM VITAE

Dr. Abdul Aziz B. Mohamed Yusoff

azizmdy@yahoo.com

ACADEMIC QUALIFICATIONS

2004 2007 : Doctor of Philosophy (Ph.D) (Molecular Genetics-Mitochondria)

Newcastle University, UK

1999 2002 : Master Of Science (M.Sc) (Human/Medical Genetics)

Universiti Sains Malaysia

1993 1997 : Bachelor Of Biomedical Science (B.Biomed.Sc) With Honours

Universiti Kebangsaan Malaysia

WORK EXPERIENCE

2008-Date : -Lecturer at Department Neurosciences, USM

Feb 2002-Feb 2003: -Research Officer for MAKNA-USM-UPM project at Human Genome
Centre and Department of Neuroscience, Health Campus, Universiti
Sains Malaysia, 16150 Kubang Kerian, Kelantan.

Jan 1999-Mac 1999: -Medical Lab Scientist at Gribbles Pathology Sdn. Bhd Tingkat 1, Lot
No. 1002, Jalan Tok Hakim,15000 Kota Bharu, Kelantan.

Dec 1997-Oct 1998: -Lab manager/biochemist at Akudia Sdn. Bhd., Makmal Klinikal &
Penjagaan Kesihatan, Lot PT 138, Sutera Inn, Jalan Dusun Muda,
15400 Kota Bharu, Kelantan.

TEACHING EXPERIENCE:

1999-2001: -Laboratory demonstrator for 3rd year Medical Lab Technology Course
(MLT), School of Medical Sciences, USM

-Laboratory demonstrator for 2nd year Biomedical Course, School of

Health Science, USM

- Teaching on basically about Molecular Genetic of Brain tumors for 1st

year Master of Neurosurgery Course, School of Medical Science, USM

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EDUCATION AWARD(S):

2002: Academic Staff Training Scheme (ASTS) Fellowship by Universiti Sains

Malaysia

WORKSHOPS/COURSES/SEMINARS ATTENDED:

1997: Research Methodology, Biostatistics, Statistical Software Application and Data

Management For Health Sciences Researchers organized by Association of
Medical Laboratory Sciences (AMels), School of Medical Sciences, USM, 18-
20th Mac, 1997

1999: Participate in workshop on method in gene analysis II: DNA sequencing &
analysis (11th-22nd Oct. 1999) organized by UKM-MTDC Biotechnology
Academy, MBCC and CGAT, UKM.

2000: Seminar Gene Mutation Screening (6th July 2000) organized by Medical
Genetic Unit at School of Medical Sciences, Universiti Sains Malaysia,
Kelantan.

2002: Attend in the presentation on Karyotyping Workstation (Training date: 29

July 2002) Training provided by Hi-TECH Instruments Sdn. Bhd.

Participate in CMA Intracranial Microdialysis Training (17th-19th Sept. 2002)

organized by Neuro Intensive Care Unit, Hospital Universiti Sains Malaysia.

Contribute and participate in the Introduction to Neurosciences (26th Oct.

2002) organized by Neurosciences Unit, School of Medical Sciences,
Universiti Sains Malaysia

Participate in the Management of Nude Mice (22nd-23rd Dec. 2002) organized

by Neurosciences Unit, School of Medical Sciences, Universiti Sains Malaysia

2003: Participate in Animal Cell Culture Workshop (6-9 Jan. 2003) organized by
Laboratory of Molecular and Cell Biology, Institute of Bioscience, Universiti
Putra Malaysia

Participate in the Advances in Laboratory Animal Science Seminar (27 th

March, 2003) organized by Laboratory Animal Science Association of
Malaysia

Participate in the Pediatrics Genetics Seminar (9 April 2003) held at Health

Campus Universiti Sains Malaysia in Collaboration with School of Medicine,
Kobe Universiti Kobe, Japan & Takatsuki General Hospital Osaka, Japan

Participate in Intellectual Property Rights & Commercialisation in Life

Sciences (5-8 May 2003) jointly organized by Ministry of Science, Technology
and The Environment (MOSTE) & Universiti Putra Malaysia.

2005: Attachment for 2 weeks at Institut de biologie Moleculaire des plantes,

Strasbourg, France. (May 2005)

Introductory Bioinformatics Course 2005 (Netskills Training Suite, Herschel

Building 26-28 Sept 2005)

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CONFERENCES ATTENDED:

1999: 5th National Conference on Medical Sciences (NCMS), School of Medical

Sciences at Universiti Sains Malaysia, Kelantan. (4-5th May, 1999)

2001: 1st ASEAN Conference on Medical Sciences (ACMS), School of Medical

Sciences, USM in collaboration with Indonesia-Malaysia-Thailand Growth
Triangle (IMT- GT), (18-21 May 2001) at Renaissance Hotel Kota Bharu,
Kelantan, Malaysia.

2003: 8th National Conference on Medical Sciences (NCMS) Medicine In Genome

Era, organized by School of Medical Sciences, Health Campus, Universiti
Sains Malaysia, Kelantan. (8-9th May, 2003)

2006: Postgraduate Research Conference 2006, The Medical School, Newcastle

University.

PRESENTATIONS (FIRST PRESENTER OR CO-AUTHORS):

1) Analysis of p53 gene mutations using non radioactive PCR-SSCP in cases of

gliomas and meningiomas in East Coast of West Malaysia. N. Atifah Adam,
A.Aziz Yusoff, M.N.Isa, J. Abdullah, M. Ros Sidek, S.F Ramli, Khairani I.M.
Proceedings the 10th Malaysian Institute of Medical Laboratory Sciences national
Scientific Conference Hilton Kuala Lumpur, 11-12th Oct. 1999, pg 18-19.

2) A non isotopic Polymerase Chain Reaction (PCR)-based technique for

identification of Fragile X. M. Ros Sidek, S.Fatimah Ramli, N. Atifah Adam, W.
Asma, , A. Aziz Yusoff, M.N. Isa. Proceedings the 10th Malaysian Institute of Medical
Laboratory Sciences national Scientific Conference Hilton Kuala Lumpur, 11-12th Oct.
1999, pg 18-19.

3) Non-radioactive PCR-SSCP for detect on of p53 gene mutation in brain

tumours: A preliminary results in Congress of neurological Surgeon 1999, Boston
MA Oct 30-Nov 4 1999.

4) p53 gene mutations in Glioma patients in Malaysia.

A.A. Yusoff , M.R. Sidek, S.F. Ramli, N.A. Adam, K.I. Mokhtar, J. Abdullah & M.N. Isa.
(Abstract Book 1st Asean Conference on Medical Sciences 18-21 May 2001 pg. 87.
Presented as a poster at Renaissance Hotel Kota Bharu, Kelantan, Malaysia).

5) Deletion of the multiple tumor suppressor gene 1 (MTS1/p16) at exon 2 in the

progression of human gliomas in East Coast of West Malaysia.
S.Sulong, A.A. Yusoff, N. Zainuddin, M.R. Sidek, S.F. Ramli, N.A. Adam, J. Abdullah &
M.N. Isa.
(Abstract Book 1st Asean Conference on Medical Sciences 18-21 May 2001 pg. 65.
Presented as a poster at Renaissance Hotel Kota Bharu, Kelantan, Malaysia).

6) Loss of heterozygosity on chromosome 10q in East Coast Malaysian patients

with Glioma
N. Zainuddin, M.R. Sidek, S.F. Ramli, N.A. Adam, K.I. Mokhtar , A.A. Yusoff, J.
Abdullah & M.N. Isa.
(Abstract Book 1st Asean Conference on Medical Sciences 18-21 May 2001 pg. 92.
Presented as a poster at Renaissance Hotel Kota Bharu, Kelantan, Malaysia).

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7) p53 gene mutations detected in glioma patients in Malaysia
Abdullah J, Yusoff AA, Sidek MR, Ramli SF, Adam NA, Mokhtar KI, Isa MN
Accepted for poster presentation at 12th World Congress of Neurosurgery, 16-20
Sept. 2001, Sydney, Australia. Pg. 316.

8) Mutations of p53 tumor suppressor gene in progression of gliomas in the

Malaysian population
A.A. Yusoff, M.R. Sidek, S.F., Ramli, N.A. Adam, K.I. Mokhtar, J. Abdullah & M.N. Isa
Accepted for oral presentation at Malaysian Science and Technology Congress 2001
(MSTC 2001) Symposium A : Life Sciences, 24-26 Sept. 2001, Kota Kinabalu
Sabah. Pg. 190.

9) Mutational analysis of the p53, p16 and PTEN genes in human gliomas
M. N. Isa., A. A. Yusoff, N. Zainuddin, S. Sulong & J. M. Abdullah
Accepted for poster presentation at 4th HUGO Pacific Meeting & 5th ASIA-Pacific
Conference on Human Genetics, 27-30 October 2002, Ambassador City Jomtien
Pattaya, Chonburi Thailand.

10) The Lack of a Role for Ras gene in human Gliomas in Malaysian patients
A.A. Yusoff, J. Abdullah, H. Jaffar, I. Aini, A. Manaf, Y. Khatijah, O. Abdul Rahman,
M.L. Mohd Azmi, O. Fauziah, M.M. Noordin, M. Grisso & M.N. Isa
Accepted for poster presentation at 8th National Conference on Medical Sciences
Medicine in the Genomic Era, 8-9 May 2003, Health Campus, Universiti Sains
Malaysia, Kelantan.

11) Identification of the human mitochondrial valyl-tRNA synthetase

A Aziz Mohamed Yusoff, ZMA Chrzanowska-Lightowlers, RN Lightowlers
Accepted for poster presentation at Postgraduate Research Conference 2006,
Newcastle University.
*(This poster had been awarded as a Best Poster in this conference)

12) Identification of the human mitochondrial valyl-tRNA synthetase and the role of
mutated mitochondrial tRNA in disease
A Aziz Mohamed Yusoff, ZMA Chrzanowska-Lightowlers, RN Lightowlers
MRG Meeting 2006, Newcastle University

PUBLICATIONS, PROCEEDING & MANUSCRIPT

Overexpression of human mitochondrial valyl tRNA synthetase can partially restore

levels of cognate mt-tRNAVal carrying the pathogenic C25U mutation.
Rorbach J, Yusoff AA, Tuppen H, Abg-Kamaludin DP, Chrzanowska-Lightowlers ZM, Taylor
RW, Turnbull DM, McFarland R, Lightowlers RN.
Nucleic Acids Res. 2008 May;36(9):3065-74. Epub 2008 Apr 8.

Molecular genetic analysis of a supratentorial haemangioblastoma in a non-Von Hippel

Lindau patient.
Zamzuri I, Ghazali MM, Zainuddin N, Sulong S, Samarendra SM, Yusoff AA, Ariff AR,
Abdullah J.
Med J Malaysia. 2005 Aug;60(3):360-3.

Absence of Ras, c-myc and epidermal growth factor receptor (EGFR) mutations in
human gliomas and its clinical factors associated with pathological grading after
six years of diagnosis in North East Malaysian patients
MM Ghazali, MSM Zan, AA Yusof, JM Abdullah et al.
Malaysian Journal of Medical Sciences. Vol. 12, No. 2, July 2005 (27-33).

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Association of p53 tumor suppressor gene with paraclinical and clinical modalities
of gliomas patients in Malaysia.
Yusoff AA, Abdullah J, Abdullah MR, Mohd Ariff AR, Isa MN.
Acta Neurochir (Wien). 2004 Jun;146(6):595-601. Epub 2004 Apr 8.

What we know about the molecular genetics of central nervous system (CNS) tumours
in Malaysia
Sarina Sulong, Abdul Aziz Mohamed Yusoff, Norafiza Zainuddin, Jafri Malin Abdullah,
Jain George Pannatil, Hasnan Jaafar, Mohd Nizam Isa
Malaysian Journal of Medical Sciences. Vol. 11, No. 1, January 2004 (37-43)

Mutations of the p53 gene in gliomas from Malay patients

M Y Aziz, A Jafri, S Ros, I Nizam,
The Medical Journal of Malaysia. 2003. Vol. 58(2): 225-232

Molecular Genetic Analysis of Anaplastic Pleomorphic Xanthoastrocytoma

Noor Azam Nasuha, Abd. Hamid Daud, Mazira Mohamad Ghazali, Samarendra Singh Mutum,
Biswa Mohan Biswal, Abdul Rahman Mohd Ariff, Abdul Aziz Yusoff, Sarina Sulong, Norafiza
Zainuddin, Jafri Malin Abdullah & Mohd. Nizam Isa
Asian Journal of Surgery. 2003. Vol. 26(2): 120-125

Non-isotopic PCR-cold SSCP of p53 gene mutations in Gliomas using the Dcode
System
M.N. Isa, A.A. Yusoff, M.R. Sidek, J.M. Abdullah
Asia Pacific Journal of Molecular Biology & Biotechnology. 2001. Vol. 9(2): 107-110

p53 gene mutations detected in glioma patients in Malaysia

Abdullah J, Yusoff AA, Sidek MR, Ramli SF, Adam NA, Mokhtar KI, Isa MN
Proceeding of 12th World Congress of Neurosurgery, 16-20 Sept. 2001, Sydney,
Australia. (Neurosurgical Society of Australasia Research Foundation).pg. 472-473.

Is there p16 mutation in Giant Nerve Sheath tumour : Report of three cases
Abdullah JM, Sulong S, Yusoff AA, Arif AR, Biswal BM, Gurjeet K, Jaafar H, Samarendra S,
Abdullah MR, Is MN
Proceeding of 12th World Congress of Neurosurgery, 16-20 Sept. 2001, Sydney,
Australia. (Neurosurgical Society of Australasia Research Foundation).pg. 465-466.

Mutations of p53 tumor suppressor gene in progression of gliomas in the Malaysian

population
A.A. Yusoff, M.R. Sidek, S.F., Ramli, N.A. Adam, K.I. Mokhtar, J. Abdullah & M.N. Isa
Manuscript in Malaysian Science and Technology Congress 2001 (MSTC 2001)
Symposium A : Life Sciences, 24-26 Sept. 2001, Kota Kinabalu Sabah

THESIS

Abdul Aziz Mohd Yusoff (2002). Molecular genetic analysis and

Immunohischemistry of the p53 tumor suppressor gene in brain tumor patients
from Malaysia ; Universiti Sains Malaysia (M.Sc thesis)

Abdul Aziz Mohamed Yusoff (2007). Identification of the human mitochondrial

valyl-tRNA synthetase and demonstration of its role in suppressing a
pathogenic mutation in the cognate mitochondrial tRNA Val. Newcastle University
(Ph.D thesis)

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LABORATORY PRACTICAL SKILLS:

Having experiences in:

- Molecular biology experimental procedures

- DNA extraction from blood and tissue using non-phenol salting out
procedure or commercial extraction kits
- PCR amplification technique
- Gel electrophoresis analysis by agarose gel and polyacrylamide (PAGE)
- Mutational analysis by SSCP technique
- Silver staining procedures
- Immunohistochemistry analysis using strep avidin-biotin complex
technique
- Cell culture and cytogenetic technique
- DNA sequencing technique
- Molecular cloning
- Western and Northern Blot
- In vitro aminoacylation assay

Others: - Have some theoretical and practical knowledge in laboratory instruments

such as
- Dcode Universal Mutation Detection System
- Automated DNA Sequencing (ABI PRISM 377 & 3100)
- Electrophoresis instruments
- IMX System (HIV I & II AB, Hepatitis B AG & AB, ect)
- Cell-DYN 1400
- RA-50 Chemistry Analyzer
- Spectrophotometer (UV)
- Atomic Absorption Spectrophotometry
- HPLC
- Electron Microscopy

- Have a fairly good basic theoretical knowledge in:

Laboratory Safety, Quality Assurance and Good
Manufacturing Practices (GMP), and also in Biochemistry,
Microbiology and Immunology, Pharmacology, Pathology,
Anatomy. Parasitology and Community Health.