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Thesis Title Detection of Apomixis in Lansium domesticum Corr.
Author Miss Pharichart Khongsuwan
Major Program Plant Science
Academic Year 2005

ABSTRACT

Detection of apomixis in Lansium domesticum Corr. was carried out by

cytoembryological method and RAPD (Random Amplified Polymorphic DNA) technique. Flower
of longkong langsat and duku were collected from experimental plot at the Department of
Plant Science, Faculty of Natural Resources, Prince of Songkla University, while leaf and seed
samples were collected from farmerts orchards at Narathiwat and Songkhla province. For
cytoembryological analysis, flowers at various stages were sectioned at 8 micrometer, stained and
embryo sac development was observed under compound microscope. At the receptive stage,
multiple embryo sac in the same ovule were found in longkong and langsat indicated aposporous
apomixis. However, multiple embryo sac was not detected in duku. DNA patterns of longkong,
langsat and duku seedlings including their maternal parents were revealed by RAPD with the
following eight primers : OPA-10 (GTGATCGCAG), OPB-04 (GGACTGGAGT), OPB-07
(GGTGACGCAG), OPC-04 (CCGCATCTAC), OPC-05 (GATGACCGCC), OPD-03
(GTCGCCGTCA), OPT-01 (GGGCCACTCA) and OPT-08 (AACGGCGACA). Of total 149
longkong seedlings from different 3 maternal plants exhibited identical DNA fragment profiles. It
was concluded that all longkong seedlings from this study derived from somatic cells and
confirmed obligate apomixis. While 71.4 % of langsat and only 12.8 % of duku seedlings have
DNA patterns like their maternal plants. These results indicated that 28.6 % and 88.2 % of langsat
and duku seed originated from fusion between sperm and egg cell and confirmed facultative
apomixis. Four and twenty-four genotypes can be distinguished within langsat and duku seedling
populations, respectively. Based on the RAPD patterns obtained with 8 primers from this study,
indicating longkong can be propagated true-to-type by seed whereas propagation by seed in langsat
and duku produce different genotype of seedlings.

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