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INTRODUCTION
BIO-ELECTROFLOTATION
Electrochemistry
pH
Current density
Electrode material
Redox
reactions
Bio-
Eletroflotation
Surface
Biology
chemistry
Bioflotation Bioreagents
Decomposition of water into oxygen and hydrogen gas bubbles due to an electric
current being passed through the water.
The reaction at the anode (+) is:
2H 2 O 4H O2( g ) 4e (1)
Production of microbubbles;
Absence of coalescence of bubbles;
Production of bubbles of desired size and flux;
Higher chance of bubble particle collision;
Activeness;
C D
Figure 4 Micrographs (A, B) and schematic outline of the cell wall structures(C, D). Gram-positive (A, C); Gram-
negative (B, D). (Mesnage, et al 1998; Beveridge, 1999). 1) Plasma membrane; 2) peptidoglycan; 3) proteins; 4)
fibrils; 5) periplasmic space; 6) outer membrane.
Use of microorganisms in industrial process
Lixiviation and oxidation, remediation, removal of heavy metals (Sorption) and flotation.
Thioether >S S AA
Amide -C=O-NH2 N AA
Imine =NH N AA
Imidazole C3H4N2 N AA
Phosphonate -P=O-(OH)2 O FL
10
12
1 2 3
6 7
11
4 5
8
H2 O2
80 80
d32(m)
d32(m)
65 65
60 60
55 55
50 50
2 3 4 5 6 7 8 9 10 11 12 2 3 4 5 6 7 8 9 10 11 12
pH pH
Figure 7 Effect of pH in bubble size. A) Hydrogen bubbles; B) Oxygen bubbles . Sodium sulfate concentration: 0.05 M.
Zeta potential
25
20 R. opacus
Hematite
15
Hematite + R. opacus
10
5
0
Zeta potential (mV)
-5
IEP
-10
IEP IEP
-15
-20
-25
-30
-35
-40
-45
1 2 3 4 5 6 7 8 9 10 11 12
pH
Figure 8 Zeta potentials of hematite before and after interaction with Rhodococcus opacus.
Hematite electroflotation
70 70
65 A Concentration of bioreagent 65 B Concentration of bioreagent
60 100 mg/L 60 100 mg/L
55 200 mg/L 200 mg/L
55
300 mg/L 300 mg/L
50 50
45 45
Floatability (%)
Floatability (%)
40 40
35 35
30 30
25 25
20 20
15 15
10 10
5 5
0 0
2 3 4 5 6 7 8 9 10 11 12 2 3 4 5 6 7 8 9 10 11 12
pH pH
The zeta potential evaluation of the mineral particles before and after the R.
opacus interaction showed that the bioreagent modified the zeta potential
profiles of hematite.