NewVITEKMSSystemtoIdentify193SpeciesofBacteriaandYeastsRapidly

Learn about VITEK MS identification system for identification of pathogenic microorganisms developed by BioMerieux.
Pathogens in finished products and environmental flora must be identified in pharmaceutical manufacturing up to species level. According to FDA every
bacterium in manufacturing environment should be identified and every new microbe should be identified when it is caught first time during environmental
monitoring.

VITEK MS System
Identification by traditional method selective media always remains questionable because method does
not provide supporting data i.e. printouts by 21 CFR compliance software with date, authentic
photographs etc. These selective media methods take five days for final results of identification.

Due to all these problems an authentic and rapid method is required to identify the environmental isolates.
BioMerieux, Inc. recently developed such a method to identify the 193 pathogenic species of bacteria and
yeasts. Yeasts from Malassezia, Cryptococcus and Candida groups and bacteria from Staphylococcaceae,
Streptococcaceae, Enterobacteriaceae, Pseudomonadaceae, and Bacteroidaceae families those are
responsible for many serious human infections are easily identified with this VITEK MS system.

VITEK MS system is based on matrixassisted laser desorption/ionizationtime of flight mass spectrometry

MALDITOF MS technology. System breaks the cells of bacteria and yeasts into small particles. The pattern of particles is compared with the 193 saved
patterns in system database. Identification is done in three simple steps 1 placement of sample directly onto the target slide 2 addition of ready made
matrix solution and 3 analysis of sample within minutes. System can analyze 4 sample slides with 48 positions each 192 isolates in every run.

Related: SOP for Biomerieux Kit

Identification Process

A small number of cells are required for identification unlike the other methods those require dense colonies. So testing can be started when growth starts
after 1824 hours of incubation.

FDA approved the VITEK MS system after finding the results of a study on 7,068 microorganisms.
FDA says When compared to sequencing and biochemical testing, the VITEK MS correctly identified the scientific group or family 93.6 percent of the time (with
87.5 percent of microorganisms identified to species level). The VITEK MS provided a `no identification` result for 3.2 percent of the microorganisms in the
study. Of all test results, only 0.8 percent were incorrect and 2.4 percent were low discrimination with no correct result."

VITEK MS system will help to identify the microbial contaminants up to species level in pharmaceuticals and also be helpful in identification of human
specimen of critically ill patients for bacterial and fungal infections in very short time.

Also see: SOP for Identification of Microbes using Biomerieux Identification System

Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of Pharmaceutical Guidelines, a widelyread pharmaceutical
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