Animal Nutrition - Norel

Norel
Animal Nutrition feeding life

Norel
Miguel Colilla Commercial Director APAC Penang, Aug 17
1 Norel, S.A. CIF: A-28617801 C/Jess Aprendiz, 19 1 A y B, 28007 Madrid SPAIN +34 915 014 041 www.norel.es norel@norel.es
GUT HEALTH_FEED ADDITIVES PATHOGENS CONTROL
_
GUSTOR NRGY
GUSTOR NRGY
Efficacy of protected sodium butyrate against
Salmonella in broilers (Adapted from Puyalto et al., 2016)
Puyalto2016
M. Puyalto1, C. Sol1, P. Honrubia1, Y. Prez2, R. Balaa-Fouce2, M.J. Villamide3 and J.J. Mallo1
1 NOREL S.A., Madrid, Spain.

1 NOREL S.A
2 Dpt. Ciencias Biomdicas. Universidad de Len, Len, Spain.
2
3 Dpt. Produccin Agraria. Universidad Politcnica de Madrid, Madrid, Spain
3
Norel, S.A. CIF: A-28617801 C/Jess Aprendiz, 19 1 A y B, 28007 Madrid SPAIN +34 915 014 041 www.norel.es norel@norel.es
GUSTOR NRGY
Puyalto2016
Objective:
A trial was conducted to evaluate the preventive effect of GUSTOR NRGY against
S. Enteritidis infection in broilers, on fecal shedding and in organs content.
GUSTOR N'RGY,
Material and Methods:

Animals: 80 Cobb 1-day-old males broiler chickens. The study lasted 42 d
80Cobb 1 42
1) Treatments
No additive addition
Gustor NRGY (1.3 kg/t of feed) Gustor N'RGY1.3/
GUSTOR NRGY
Puyalto2016
2) Measurements
Presence of Salmonella in feces with cloacal swabs and microbial counts in organs of a 40% of
birds in each treatment (crop, ceca, liver and spleen)
40

5 d Challenge 5
Sampling
1 d 6 d 12d 19 d 26 d 33 d 41 d days

Euthanasia

20% Birds inoculated with 106
CFU S. Enterica CECT 4156
Organs
20106 CFU S. sampling
Enterica CECT 4156
GUSTOR NRGY
Results: Presence of Salmonella in feces

% of positive animals to Salmonella in cloacal swabs

Positive animals (%) (%)
100
*
80 *
*
60
*
40 *
20
0
6d 12d 19d 26d 33d 41d
Control Gustor N'RGY

* Differences between treatment and Control (P<0.05)
* P <0.05
GUSTOR NRGY
Results: Presence of Salmonella in organs

% of organs affected by Salmonella

Organs affected (%)
100
a a
a
80
60
b
40
b
20
b
0
Ceca Crop Spleen Liver
Control Gustor N'RGY
GUSTOR NRGY
Conclusions
It can be concluded that GUSTOR NRGY reduces the

presence of Salmonella in feces and is able to prevent
systemic infection in broilers experimentally challenged
with Salmonella Enteritidis.
GUSTOR N'RGY

(GUSTOR BP70)(NATESSE)

1Babolna Hybrid

25

Gholiamiandehkordy
(10)
1.
16 18192021 3-6 x 105

CFU 19 10
371014172023
25 Gholamiandehkordi
et al. (2007)0= 1=2= (1-2cm) ; 3=
(3-4cm)4=

NATESSE ()
17
p<0.05)
25

NATESSE

(p<0.07)
(Table 4)
17 23
Sodium salt of coconut
fatty acid distillate

FA composition
Min 67% crude fat 60
67
50
40
Lauric
30 Capric
Caprilic
20 Others
10
0
Coconut oil
In vitro Trials
Morphological changes in C.
perfringens after 30 min incubation of
cells with lauric acid at 1 mg /ml.
1/
30
Although there was no visible Normal

alteration in the cell wall structure and
size of cells, the separation of inner
and outer membranes and complete
cytoplasm disorganization in lauric
acid-treated cells are apparent

Cytoplasm disorganization

Skrivanova et al., 2005
In vitro Trials: MIC and MBC
MIC: Minimal inhibitory concentration of a product that inhibits the
growth of a microorganism (bacteriostatic activity)

MBC: Lowest concentration of a product with antimicrobial activity

able to kill the 99.9% of microorganisms (bactericide)
2400 ppm
1200 ppm
No growth
600 ppm
MIC: No visible growth
300 ppm

150 ppm
Growth 75 ppm
Positive Control
Negative Control
Product X
1, 2, 3 replicates
CDTI MIMBIOPOR, 2016
MIC: Minimal inhibitory concentration of a product that
inhibits the growth of a microorganism (bacteriostatic activity)
MBC: Lowest concentration of a product with antimicrobial

activity able to kill the 99.9% of microorganisms (bactericide)
99.9
Product X (300 ppm)
1, 2, 3 replicates
Growth
No growth
Product X (600 ppm) MBC: No colonies of

1, 2, 3 replicates microorganisms


In vitro Trials: Material and Methods

Strains evaluated
Cl. Perfringens
E. Coli Salmonella

Identification Haemolysis Identification Serotype Identification

Cholerasuis
CECT 434 + CECT 915 CP 3

EC 58 + CECT 4300 Enteriditis CP 34
EC 59 + CECT 700 Infantis CP 52

Typhimurium
EC 60 + CECT 443 CP 89

Typhimurium
EC 61 + CECT 883 CP 99

Typhimurium
EC 67 + SP 11 CP H

EC 96 + SP 28 London
EC 107 +
16 EC 115 + Norel, S.A. CIF: A-28617801 C/Jess Aprendiz, 19 1 A y B, 28007 Madrid SPAIN +34 915 014 041 www.norel.es norel@norel.es

RESULTS: MIC (ppm) PPM
E. Coli Salmonella Cl. Perfringens
Product

pH 4.8 pH 6 pH 4.8 pH 6 pH 4.8 pH 6
B98 512 50000 256 125000 nd2 31250
Dicosan 1024 > 50001 256 > 50001 nd2 7.68
RESULTS: MBC (ppm) PPM
E. Coli Salmonella Cl. Perfringens
Product

pH 4.8 pH 6 pH 4.8 pH 6 pH 4.8 pH 6
B98 62500 62500 125000 125000 nd2 62500
Dicosan >50001 >50001 256 >50001 nd2 7.68
1 It was not possible to start at higher concentration
At low pH, bacteria are more sensible to the products tested
2 Cl. Perfringens did not grow at ph 4.8
pH
DICOSAN had higher bacteriostatic and bactericide effect against Cl. Perfringens
DICOSAN CDTI MIMBIOPOR, 2016
THANK YOU VERY MUCH FOR
YOUR ATTENTION

Animal Nutrition - Norel

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Norel

Animal Nutrition feeding life

1 NOREL S.A., Madrid, Spain.

Material and Methods:

Control Gustor N'RGY

Control Gustor N'RGY

It can be concluded that GUSTOR NRGY reduces the

16 18192021 3-6 x 105

Although there was no visible Normal

MBC: Lowest concentration of a product with antimicrobial activity

MBC: Lowest concentration of a product with antimicrobial

Product X (600 ppm) MBC: No colonies of

CDTI MIMBIOPOR, 2016

EC 58 + CECT 4300 Enteriditis CP 34

EC 59 + CECT 700 Infantis CP 52

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