Materials & Streams Report September 11, 2017

for Tutorial fermentasi

1. OVERALL PROCESS DATA

Annual Operating Time 7,903.23h

Recipe Batch Time 32.52h
Recipe Cycle Time 32.52h
Number of Batches per Year 243.00
MP = Undefined

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2.1 STARTING MATERIAL REQUIREMENTS (per Section)

Amount
Gross
Needed Molar Mass
Mass
Section Starting Material Active Product (kg Yield Yield
Yield
Sin/kg (%) (%)
(%)
MP)
Fermentation (none) (none) Unknown Unknown Unknown Unknown

Sin = Section Starting Material, Aout = Section Active Product

2.2 BULK MATERIALS (Entire Process)

Material kg/yr kg/batch kg/kg MP

Air 1,835,732 7,554.454
Biomass 1,215 5.000
Media 91,125 375.000
Water 1,895,400 7,800.000
TOTAL 3,823,472 15,734.454

2.3 BULK MATERIALS (per Section)

SECTIONS IN: Fermentation Reactor

Fermentation
Material kg/yr kg/batch kg/kg MP
Air 1,835,732 7,554.454
Biomass 1,215 5.000
Media 91,125 375.000
Water 1,895,400 7,800.000
TOTAL 3,823,472 15,734.454

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2.4 BULK MATERIALS (per Material)

Air
Procedure % Total kg/yr kg/batch kg/kg MP
Fermentation (Fermentation Reactor)
P-1 100.00 1,835,732 7,554.454
TOTAL 100.00 1,835,732 7,554.454

Biomass
Procedure % Total kg/yr kg/batch kg/kg MP
Fermentation (Fermentation Reactor)
P-1 100.00 1,215 5.000
TOTAL 100.00 1,215 5.000

Media
Procedure % Total kg/yr kg/batch kg/kg MP
Fermentation (Fermentation Reactor)
P-1 100.00 91,125 375.000
TOTAL 100.00 91,125 375.000

Water
Procedure % Total kg/yr kg/batch kg/kg MP
Fermentation (Fermentation Reactor)
P-1 100.00 1,895,400 7,800.000
TOTAL 100.00 1,895,400 7,800.000

2.5 BULK MATERIALS: SECTION TOTALS (kg/batch)

Raw Material Fermentation

Air 7,554.454
Biomass 5.000
Media 375.000
Water 7,800.000
TOTAL 15,734.454

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2.6 BULK MATERIALS: SECTION TOTALS (kg/yr)

Raw Material Fermentation

Air 1,835,732
Biomass 1,215
Media 91,125
Water 1,895,400
TOTAL 3,823,472

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3. STREAM DETAILS

Stream Name Water Media Air Vent

Source INPUT INPUT INPUT P-1
Destination P-1 P-1 P-1 OUTPUT
Stream Properties
Activity (U/ml) 0.00 0.00 0.00 0.00
Temperature (C) 25.00 25.00 25.00 37.00
Pressure (bar) 1.01 1.01 1.01 1.01
Density (g/L) 994.70 995.39 1.18 1.13
Total Enthalpy (kW-h) 226.23 11.02 53.12 80.93
Specific Enthalpy (kcal/kg) 24.96 24.96 6.05 9.21
Heat Capacity (kcal/kg-C) 1.00 1.00 0.24 0.24
Component Flowrates (kg/batch)
Biomass 0.000 5.000 0.000 0.000
Carb. Dioxide 0.000 0.000 0.000 35.376
Media 0.000 375.000 0.000 0.000
Nitrogen 0.000 0.000 5,795.156 5,802.659
Oxygen 0.000 0.000 1,759.297 1,726.199
Water 7,800.000 0.000 0.000 0.000
TOTAL (kg/batch) 7,800.000 380.000 7,554.454 7,564.233
TOTAL (L/batch) 7,841.526 381.758 6,406,305.316 6,665,095.998

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Stream Name Broth
Source P-1
Destination OUTPUT
Stream Properties
Activity (U/ml) 0.00
Temperature (C) 5.00
Pressure (bar) 0.82
Density (g/L) 1,002.10
Total Enthalpy (kW-h) 47.45
Specific Enthalpy (kcal/kg) 4.99
Heat Capacity (kcal/kg-C) 1.00
Component Flowrates (kg/batch)
Biomass 19.152
Impurity 5.054
MAb 1.011
Media 324.456
Water 7,830.326
TOTAL (kg/batch) 8,180.000
TOTAL (L/batch) 8,162.845

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4. OVERALL COMPONENT BALANCE (kg/batch)

COMPONENT INITIAL INPUT OUTPUT FINAL IN-OUT

Biomass 0.000 5.000 19.152 0.000 - 14.152
Carb. Dioxide 0.000 0.000 35.376 0.005 - 35.381
Impurity 0.000 0.000 5.054 0.000 - 5.054
MAb 0.000 0.000 1.011 0.000 - 1.011
Media 0.000 375.000 324.456 0.000 50.544
Nitrogen 8.302 5,795.156 5,802.659 0.800 0.000
Oxygen 2.520 1,759.297 1,726.199 0.238 35.381
Water 0.000 7,800.000 7,830.326 0.000 - 30.326
TOTAL 10.822 15,734.454 15,744.233 1.042 0.000

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5. EQUIPMENT CONTENTS

FR-101
Procedure Operation Time (in h) Volume (in L)
P-1 START 0.000 0.000
P-1 CHARGE-1 (Charge) 1.390 7,841.526
P-1 CHARGE-2 (Charge) 2.357 8,223.285
P-1 HEAT-1 (Batch Heating) 2.840 8,259.583
FERMENT-1 (Batch Kinetic
P-1 26.840 8,258.771
Fermentation)
P-1 COOL-1 (Batch Cooling) 27.990 8,162.845
P-1 TRANSFER-OUT-1 (Transfer Out) 32.524 0.000

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