SCIENCE LAB REPORT

PURPOSE

The purpose of this lab was to learn more about human evolution, PCR, and Gel
electrophoresis. We are doing this because so we can learn about the structure, and the
human evolution.

HYPOTHESIS

My hypothesis was that we were going to determine the DNA and find out where we
came from. I believed the DNA would be larger than expected, and obvious on where we
came from.

PROCEDURE

On the first day, we took DNA from ourselves by having salt water in our mouths for 30
seconds. On the second day, we created a PCR reaction and moved it to the primer and
master mix into the ice. Third day, we inserted our PCR reaction into our gel. The fourth
day, we had the opportunity to see our Alu repeats. More information and how we did it
is found on BABEC (Bay Area Bioscience Education Community)

DATA/OBSERVATIONS

Dont put the complex in the DNA, put the DNA in the complex
The DNA was mixed with master mix, primer, and loading dye.

My hypothesis was incorrect, our results are small.

Ways to improve the lab is to get a better understanding of what we are doing. When we
had to put our DNA in the ice, make sure the ice is crushed next time, and to also make
sure I use the correct measurements for the pipettes.

ANALYSIS/DISCUSSION

We used 2% agarose gel ran at 150U for 20min and stained using red gel for 72 hours.
Lane 1A and 2A have low bp ladder. Lane 1 B-H and 2 B-H have 20ML of a 50ML DNA/
10UL loading dye solution. Overall, my DNA in Lane 2B didnt have enough data because
I put the complex in the DNA instead of putting the DNA inside the complex, and also put
the wrong amount of loading dye in.

CONCLUSION

In conclusion, pipetting is difficult. In this experiment, we had to measure a certain

amount of DNA, complex, and loading dye with the pipette in order for the experiment to
work. We had a 50ML, 100ML, and a 1000ML pipette. The issue was, that I used the
wrong pipette to measure out the loading dye. I put too much loading dye, so you
couldnt really see the DNA. For example, we would measure out 50ML of loading dye, I
grabbed the 100ML instead of the 50ML, which messed up everything. Overall, I did get
results from the experiment, but not correct or accurate ones for me to determine where
Im from.