BF Biosciences Limited Doc. No.

: Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
1 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

COVER SHEET

Issue Issue Date Originator Amendments/Justification

00 27-06-2009 Muhammad Adnan Khan New Procedure

_______________________

Valid from : 30-06-2009

Purpose : To establish a procedure for purified and drinking water sampling.

.

Responsible for Implementation : Manager Quality Operations

Manager Quality Control/Microbiology
A.M. Microbiology
Microbiologist

Total pages : 07

Issued to : 1. Manager Quality Operations

2. Quality Control Manager/Microbiology
3. AM Microbiology
4. Quality Assurance Manager

Reference : Microbiology Manual

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
2 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

LIST OF CONTENTS

1. Objective

2. Scope

3. Responsibilities

4. Requirements

5. Sampling Procedure for Microbiological Analysis

6. Sampling Procedure for Chemical Analysis

7. Precautions

8. Training

9. Distribution

10. Related Document

11. History

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
3 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

1. OBJECTIVE

Objective of this SOP is to define a sampling procedure for purified water and drinking water
as well.

2. SCOPE

This SOP describes the execution of purified and drinking water sampling.

3. RESPONSIBILITIES

4.1. Microbiologist:

- To interface this SOP to the actual practices of flow patterns.

- To ensure that the SOP is updated & reflects the actual execution.

- To review the SOP in case of any change or whenever it is due for revision.

4.2. AM Microbiology:

- To follow the instructions laid down by this SOP.

- To keep the relevant SOPs valid and updated.

4.3. Manager Quality Operations:

- To provide adequate resources for the implementation of this SOP as define.

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
4 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

4. REQUIREMENTS
4.1. Refrigerator
4.2. Sterile screw-capped glass bottles 100 mL
4.3. Sterile latex gloves
4.4. Filtered 70% Isopropyl Alcohol spray bottle
4.5. Washed and cleaned 2.5 L bottle

5. SAMPLING PROCEDURE FOR MICROBIOLOGICAL ANALYSIS

5.1. Sampling points must be labeled properly as no any difficulty to take
representative sample.

5.2. Before sampling, properly label the sampling bottle including sampling
point number, sampling date and name.

5.3. Sampling points must be free from aerators, sieves and purification
devices.

5.4. Before sampling, wash hands thoroughly, do not open sterile sample
bottle until you are ready to proceed.

5.5. Wear sterile latex gloves; disinfect them with filtered 70% I.P.A.

5.6. Before sampling, remove the aluminium cover from the sampling point
and disinfect it with filtered 70% I.P.A. for removing viable as well as non-
viable particles from the site of sampling, if any.

5.7. Open the sampling point and drain-out the water approximately for
1 minute (about 2 Liters); ensure that there is no splattering in water flow.

5.8. Take sterile sample bottle near to the sampling point, hold it from the
bottom in left hand and with right hand slowly remove the cap, make sure

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
5 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

that the inner surface of cap should not come in contact with any surface
including fingers or hands of the individual collecting the sample.

5.9. Collect the water sample in the sterile sample bottle up to 100 mL (do not
overflow the sample bottle) from mid of the water stream.

5.10. During collection of the sample, do not allow the bottle or the water
sample in the bottle to come in contact with the sampling valve.

5.11. Place the cap on the bottle as quickly as possible without heavy
movement of sample towards cap (avoid air to trap inside the sample
bottle).

5.12. Tighten the cap securely, close the sampling valve.

5.13. Collect the sample(s) and proceed for analysis as per SOP # .
If the sample(s) are not taken immediately for analysis OR not analyze
within 30 minutes to 2 hours then store at 2 8 C in refrigerator but not
exceed 24 hours.

5.14. Ensure that the analysis is, in any case, initiated within 24 hours after
sampling.

6. SAMPLING PROCEDURE FOR CHEMICAL ANALYSIS

6.1. For chemical analysis, clean and dried sample bottles are used for
sampling.

6.2. Before sampling, properly label the sampling bottle including sampling
point number, sampling date and name.

6.3. Before sampling, wear gloves and remove the aluminium cover from the
sampling point.

6.4. Open the sampling point and drain-out the water approximately for
1 minute (about 2 Liters); ensure that there is no splattering in water flow.

6.5. Take sample bottle near to the sampling point, hold it from the bottom in
left hand and with right hand slowly remove the cap, make sure that the

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
6 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

inner surface of cap should not come in contact with any surface including
fingers or hands of the individual collecting the sample.

6.6. Rinse the bottle with the water to be sampled at least 3 times.

6.7. Collect the water sample in the sample bottle up to the mark, place cap
on the bottle as quickly as possible and ensure that no air bubble is
entrapped.

6.8. For TOC analysis, collect the sample in the separate bottle.

6.9. Collect the sample by over flowing the bottle and close with the cap
immediately.

6.10. Tighten the cap securely, close the sampling valve.

6.11. Collect the sample(s) and proceed for analysis as per SOP # .
If the sample(s) are not taken immediately for analysis OR not analyze
within 30 minutes to 2 hours then store at 2 8 C in refrigerator but not
exceed 24 hours.

6.12. Ensure that the analysis is, in any case, initiated within 24 hours after
sampling.

7. PRECAUTIONS

7.1. Always wear latex gloves either sampling for microbiological analysis OR
chemical analysis.

7.2. Ensure that there is no splattering OR any other turbulence in water

stream.

7.3. Avoid heavy movement of sample bottle (microbiological analysis) while it

is opened.

7.4. Do not touch inner surface of cap and also care must be taken during
sampling that nothing come in contact with sampling valve either it is
bottle OR water that is being sampled.

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
7 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

7.5. Water sample analysis should be initiated immediately as sample

received otherwise sample should be kept in refrigerator at 2 8 C but
not more than 24 hours.

8. TRAINING

Training about the contents of this SOP must be imparted to all the concerned
personnel once in a year and also to new employees before starting with the job and
the records documented according to training SOP.

9. DISTRIBUTION
Issuance of New Copy Retrieval of Old version

Department /
Signature Copy # Retrieved from Retrieved by
Person

Original Copy

Copy # 1

Copy # 2

Copy # 3

Copy # 4

10. RELATED DOCUMENT

NA

11. HISTORY

Microbiology
BF/SOP/Mic
 BF Biosciences Limited Doc. No. : Mic-SOP-009-00
Effective Date: 30-06-2009

SOP Review Date:

Page:
30-06-2012
8 of 8

SAMPLING OF PURIFIED WATER AND DRINKING WATER

Written By Reviewed By Approved By
Signature Signature Signature
& Date & Date & Date

Name Muhammad Adnan Khan Name Asadullah Khan Name Ejaz Ahmad
Title Microbiologist Title Assistant Manager Microbiology Title Manager Quality Operations

This is the First version.

- First Issue : JUN,2009

- Reason of composition / review new SOP

Microbiology
BF/SOP/Mic