A.

Data Pengamatan
Pembuatan kurva baku internal Paracetamol
100 mg/100 ml 1 mg/ml 1000 g/ml

Penimbangan Paracetamol
Kertas + Paracetamol = 0,6212 gram
Kertas + sisa = 0,5206 gram
Paracetamol = 0,1006 gram = 100,6 mg

Konsentrasi Paracetamol sebenarnya

100,6
Konsentrasi Paracetamol = = 1006
100

Deret Baku Koreksi Kadar

0 g/ml (blanko) 0 g/ml (blanko)
V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 0 0 . 1006 = 500 . C2
V1 = 0 l induk +500 l darah C2 = 0 g/ml

100 g/ml 100 g/ml

V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 100 49,70 .1006 = 500 . C2
V1 = 49,70 l induk + 450,3 l darah C2 = 99,99 g/ml

200 g/ml 200 g/ml

V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 200 99,40. 1051 = 500 . C2
V1 =99,40 l induk + 400,60 l darah C2 = 199,99 g/ml

300 g/ml 300 g/ml

V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 300 149,10 .1006 = 500 . C2
V1 =149,10 l induk + 350,90 l darah C2 = 299,99 g/ml
 400 g/ml 400 g/ml
V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 400 198,10 . 1006 = 500 . C2
V1 =198,80 l induk +301,20 l darah C2 = 398,58 g/ml
500 g/ml 500 g/ml
V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 500 248,50. 1006 = 500 . C2
V1 =248,50 l induk + 251,50 l darah C2 = 499,98 g/ml
600 g/ml 600 g/ml
V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 600 298,20 . 1006 = 500 . C2
V1 =298,20 l induk +201,80 l darah C2 = 599,98 g/ml

700 g/ml 700 g/ml

V1 . C1 = V2 . C2 V1 . C1 = V2 . C2
V1 . 1006 = 500 . 700 347,90 . 1006 = 500 . C2
V1 = 347,90 l induk + 152,10 l darah C2 = 699,97 g/ml

Pengukuran Kurva Baku Paracetamol

Konsentrasi Absorbansi
(ppm)

0 0,000

99,99 0,081
a = 0,0225
199,99 0,151
b = 1,0165 103
299,99 0,357

398,58 0,373 r = 0,9742

499,98 0,452 y=bx+a

599,98 0,523
= 1,0165 103 x + (0,0225)
699,97 0,750
 Perhitungan Dosis 500mg

t absorbansi Cp Ln Cp Cp' Cpres Ln Cpres

0 0,049 65,4455 4,1812
15 0,053 69,1274 4,2359 82,1646 13,0372 2,5678
30 0,005 Abs 70,9683 4,2622 80,8341 9,8658 2,2891
45 0,057 72,8093 4,2878 79,5253 8,5570 2,1467
60 0,068 82,9345 4,4181 Regresi Fase Eliminasi (t vs ln cp)
90 0,062 77,4116 4,3491 a = 4,42505
b = -1,0883 x 10-3
120 0,057 72,8093 4,2878 r = -0,9430
150 0,056 El 71,8888 4,2751 b =-k
180 0,052 68,2069 4,2225 Kel = 1,0883 x 10-3

0,693 0,693
1) t1/2 eliminasi = = = 636,77 menit~ 10,61
1,0883 x 103

2) Regresi Absorbsi
t Ln Cpres a = 2,7556
b = - 0,0140
15 2,5678 r = - 0,9829
30 2,2891
b = -k
45 2,1467 Ka = 0,0140

0,693 0,693
t1/2 absorbsi = = = 49,5 ~ 0,83
0,0140

0,014 1,0883 x 103

tmaks = = = 198 menit ~ 3,3
0,0141,0883 103
3) AUC Trapezoid
69,1274 +65,4455
015 = . (15 0) = 1009,29 g.menit/ml
2
30 70,9683+69,1274
15 = . (30 15) = 1050,72 g.menit/ml
2
45 72,8093+70,9683
30 = . (45 30) = 1078,33 g.menit/ml
2
60 82,9345+72,8093
45 = . (60 45) = 1168,08g.menit/ml
2
90 77,4116+82,9345
60 = . (90 60)= 2405,19g.menit/ml
2
120 72,8093+77,4116
90 = . (120 90) = 2253,31g.menit/ml
2
150 71,8888+72,8093
120 = . (150 120) = 2170,47 g.menit/ml
2
180 68,2069+71,8888
150 = . (180 150) = 2101,44 g.menit/ml
2
~ 68,2069
180 = 1,0883103 = 62672,88g.menit/ml

AUC total = 75909,71 g.menit/ml

4) F = 0,8

5) Vd =

12600 0,8
= = 122,01 ml
1,0883103 / 75909,71 ./

6) Cp max =

0,8 12600 3
/ 198
= x 1,088310 = 66,6001 g/ml
122,01

7) Cp = B.eKel.t A.eKa.t
Cp = 83,5169. e -1,0883 x 103 .t 15,7305 . e -0,0140 .t
Keterangan :
B = anti ln a eliminasi
A = anti ln a absorpsi
 Perhitungan Dosis 750mg

t absorbansi Cp Ln Cp Cp' Cpres Ln Cpres

0 0,045 61,7636 4,1233
15 0,067 82,0139 4,4069 233,1464 151,1325 5,0181
30 0,072 86,6163 4,4615 209,8655 123,2492 4,8142
45 0,075 89,3778 4,4929 188,9092 99,5314 4,6005
60 0,129 139,0832 4,9351 Regresi Fase Eliminasi (t vs ln cp)
90 0,125 135,4013 4,9082 A = 5,5569
b = -7,0133 x 10-3
120 0,106 117,9124 4,7699 r = -0,920
150 0,066 81,0935 4,3956
b =-k
180 0,062 77,4116 4,3491 Kel = 7,0133 x 10-3

0,693 0,693
8) t1/2 eliminasi = = = 98,81 ~ 1,65
7,0133 x 103

9) Regresi Absorbsi
t Ln Cpres a = 5,2285
b = -0,0139
15 5,0181 r = -0,9999
30 4,8142
b = -k
45 4,6005 Ka = 0,0139

0,693 0,693
t1/2 absorbsi = = = 49,86 ~ 0,83
0,0139

0,0139 7,0133 103

tmaks = = = 99,33 menit ~ 1,66
0,0139(7,0133 103 )
10) AUC Trapezoid
82,0139+61,7636
015 = . (15 0) = 1078,33 g.menit/ml
2
30 86,6163+82,0139
15 = . (30 15) = 1264,73 g.menit/ml
2
45 89,3778+86,6163
30 = . (45 30) = 1319,96 g.menit/ml
2
60 139,0832+89,3778
45 = . (60 45) = 1713,46 g.menit/ml
2
90 135,4013+139,0832
60 = . (90 60)= 4117,27g.menit/ml
2
120 117,9124+135,4013
90 = . (120 90) = 3799,71 g.menit/ml
2
150 81,0935+117,9124
120 = . (150 120) = 2985,09 g.menit/ml
2
180 77,4116+81,0935
150 = . (180 150) = 2377,58 g.menit/ml
2
~ 77,4116
180 = 7,0133 103 = 11037,83 g.menit/ml

AUC total = 29693,96 g.menit/ml

11) F = 0,8

12) Vd =

18900 0,8
= = 72,60 ml
7,0133 103 / 29693,96 ./

13) Cp max =

0,8 18900 3 /
= x 7,0133.10 99,33
= 103,7700 g/ml
72,60

14) Cp = B.eKel.t A.eKa.t

Cp = 259,019. e -7,0133 x 103 .t 186,513 . e -0,0139 .t
Keterangan :
B = anti ln a eliminasi
A = anti ln a absorpsi