22-cGAMP VacciGrade

Cyclic [G(2,5)pA(2,5)p] - STING ligand

Catalog # vac-cga22

For research use only. Not for use in humans.

Version # 13L19-MM

PRODUCT INFORMATION METHODS

Content: Preparation of stock solution (1 mg/ml):
1 mg (2 x 500 mg) lyophilized 22-cGAMP VacciGrade - Add 500 ml sterile endotoxin-free physiological water (provided)
10 ml sterile endotoxin-free physiological water (NaCl 0.9%) to 500 mg 22-cGAMP VacciGrade.
- Mix the solution by pipetting up and down.
Storage and stability:
- 22-cGAMP VacciGrade is shipped at room temperature and Working Concentration: 5 - 50 mg/mouse
should be stored at -20C. Lyophilized product is stable 1 year when
properly stored. DESCRIPTION
- Upon resuspension, prepare aliquots of 22-cGAMP VacciGrade 22-cGAMP (cyclic [G(2,5)pA(2,5)p]) is a synthetic cyclic
and store at -20C. Resuspended product is stable 6 months when dinucleotide. Cyclic dinucleotides (CDNs) are a relatively new class
properly stored. Avoid repeated freeze-thaw cycles. of adjuvants that have been shown to increase vaccine potency1.
CDNs activate innate immunity by directly binding the endoplasmic
Quality control: reticulum-resident receptor STING (stimulator of interferon genes),
- 22-cGAMP VacciGrade is a preclinical grade preparation of the activating a signaling pathway that induces the expression of
cyclic dinucleotide 22-cGAMP. It is prepared under strict aseptic interferon- (IFN-) and also nuclear factor-B (NF-B) dependent
conditions and is tested for the presence of endotoxins. 22-cGAMP inflammatory cytokines1.
VacciGrade is guaranteed sterile and its endotoxin level
is <0.005 EU/mg. 22-cGAMP binds STING and subsequently induces TBK1-IRF3
- Purity and structure controlled by LC/MS and NMR: 95% (TANK-binding kinase 1-IFN regulatory transcription factor3) -
- Biological activity is assessed by measuring induction of the dependent production of IFN-2. 22-cGAMP is similar to
interferon pathway in THP1-Blue ISG cells. 23-cGAMP in terms of STING binding and type I interferon
induction2. 23-cGAMP is a cyclic dinucleotide produced in
CHEMICAL PROPERTIES mammalian cells by cGAS (cGAMP synthase) in response to
Synonym: cyclic GMP-AMP; c-GpAp diammonium salt double-stranded DNA in the cytoplasm. Recently, it has been reported
Formula: C20H22N10O13P2 .2NH4 that 23-cGAMP is an effective adjuvant that boosts the production of
Molecular weight: 708.47 antigen-specific antibodies and T cell responses in mice3.
Solubility: 1 mg/ml in water
Source: Synthetic 1. Dubensky TW. et al., 2013. Rationale, progress and development of vaccines utilizing
Structure: O STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4):
N 131-143. 2. Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphdiester
NH linkages is an endogenous high-affinity ligand for STING. Mol Cell.51(2):226-35.
O G 3. Li XD. et al., 2013. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and
immune adjuvant effects. Science. 341(6152):1390-4.
O 5' N N NH2
NH4+ P O O
O OH
2' 2'
OH O
O O P
N N 5' O NH +
N
A O 4

N
NH2

TECHNICAL SUPPORT
InvivoGen USA (TollFree): 888-457-5873
InvivoGen USA (International): +1 (858) 457-5873
InvivoGen Europe: +33 (0) 5-62-71-69-39 www.invivogen.com
InvivoGen Hong Kong : +852 3-622-34-80
E-mail: info@invivogen.com
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TECHNICAL SUPPORT
InvivoGen USA (TollFree): 888-457-5873
InvivoGen USA (International): +1 (858) 457-5873
InvivoGen Europe: +33 (0) 5-62-71-69-39
InvivoGen Hong Kong : +852 3-622-34-80
www.invivogen.com
E-mail: info@invivogen.com