winCATS Planar Chromatography Manager

Analysis Report
Method C:\CAMAG\winCATS\Data\ayu-tlc\aflatoksin yanita utama.cme
Created by universitas indonesia 30 Juli 2007 13:53:27
Last modified by universitas indonesia 30 Juli 2007 14:09:59
SOP document
Validated Design
Description :

Analysis E:\Praktikum Anfiskim\wincats\aflatoksin yanita utama.cna

Created/used by universitas indonesia 15 Juni 2014 10:42:11
Current user universitas indonesia

Stationary phase
Executed by universitas indonesia 15 Juni 2014 10:41:11
Plate size (X x Y) 20,0 x 10,0 cm
Material TLC plates silica gel 60 F 254
Manufacturer E. MERCK KGaA
Batch
GLP code
Pre-washing Yes
Mode
Solvent name sikloheksan-toluen-dietilamin (65:25:10)
Manufacturer E. MERCK KgaA
Batch
Drying device Oven
Temperature 120 C
Time 20 Minutes
Modification No

Definitions - Quantification
Executed by universitas indonesia 30 Juli 2007 14:09:59

Calibration parameters
Calibration mode Multi level
Statistics mode CV
Evaluation mode Peak Height & Area

Samples
Sample ID: 20
Sample ID: 40
Sample ID: 60
Sample ID: 80
Sample ID: 100
Sample ID: 120
Sample ID: 140
Sample ID: 160
Sample ID: 2
Sample ID: 4
Sample ID: 6
Sample ID: 8
Sample ID: 10
Sample ID: 12
Sample ID: 14
Sample ID: 16
Window Batch Product
Substance name Rf size Regression Deviation Purity Manufacturer number Expiry date number

aflatoksin b1 0,46 0,5 mm Linear 10.0 % 1.0

aflatoksin B1 0,48 0,8 mm Linear 10.0 % 1.0

Standards absolute
Standard level1

User : universitas indonesia Approved :. . . . . . . . . . . . . . . . . . . SN 1305W003, V1.4.1

15 Juni 2014 10:42:20 Report ID : 07DE060F010A2A0B Page 1 of 11
 winCATS Planar Chromatography Manager
Substance Amount/fraction
aflatoksin b1 0,020 ng
aflatoksin B1 0,020 ng

Standard level2
Substance Amount/fraction
aflatoksin b1 0,040 ng
aflatoksin B1 0,040 ng

Standard level3
Substance Amount/fraction
aflatoksin b1 0,060 ng
aflatoksin B1 0,060 ng

Standard level4
Substance Amount/fraction
aflatoksin b1 0,080 ng
aflatoksin B1 0,080 ng

Standard level5
Substance Amount/fraction
aflatoksin b1 0,100 ng
aflatoksin B1 0,100 ng

Standard level6
Substance Amount/fraction
aflatoksin b1 0,120 ng
aflatoksin B1 0,120 ng

Standard level7
Substance Amount/fraction
aflatoksin b1 0,140 ng
aflatoksin B1 0,140 ng

Standard level8
Substance Amount/fraction
aflatoksin b1 0,160 ng
aflatoksin B1 0,160 ng

Detection - CAMAG TLC Scanner 3

Information
Application position 5,0 mm
Solvent front position 81,0 mm

Instrument CAMAG TLC Scanner 3 "Scanner3_110306" S/N 110306 (1.14.26)

Executed by universitas indonesia 15 Juni 2014 10:41:55
Number of tracks 16
Position of first track X 10,0 mm
Distance between tracks 5,0 mm
Scan start pos. Y 5,0 mm
Scan end pos. Y 81,0 mm
Slit dimensions 6,00 x 0,30 mm, Micro
Optimize optical system Light
Scanning speed: 20 mm/s
Data resolution: 100 m/step

Measurement Table
Wavelength 354
Lamp Hg
Measurement Type Remission
Measurement Mode Fluorescence
Optical filter K400
Detector mode Automatic
PM high voltage 194 V

Detector properties
Y-position for 0 adjust 5,0 mm
Track # for 0 adjust 0
Analog Offset 10%
Sensitivity Automatic (219)

User : universitas indonesia Approved :. . . . . . . . . . . . . . . . . . . SN 1305W003, V1.4.1

15 Juni 2014 10:42:20 Report ID : 07DE060F010A2A0B Page 2 of 11
 winCATS Planar Chromatography Manager

Integration
Properties
Data filtering Savitsky-Golay 7
Baseline correction Lowest Slope
Peak threshold min. slope 5
Peak threshold min. height 10 AU
Peak threshold min. area 50
Peak threshold max. height 990 AU
Track start position 38,6 mm
Track end position 43,7 mm
Display scaling Automatic
All tracks at Wavelength

500
[ AU ]
400
350
300
250
200
150
100 200

50
0 [ mm ]

0,45
0,46 100

0,47
0,48 50
0,49
[ Rf ]
0,51 0

Track 1, ID: Standard1

00 300

50 250

00 200

50 150

00 100

50 50

0 0
-0,07 0,14 0,34 0,54 0,73 0,94 -0,07 0,14 0,34 0,54 0,73 0,94

User : universitas indonesia Approved :. . . . . . . . . . . . . . . . . . . SN 1305W003, V1.4.1

15 Juni 2014 10:42:20 Report ID : 07DE060F010A2A0B Page 3 of 11