LESSON PLAN

ANALYTICAL INSTRUMENTATION

Sub Code : 10 EI 65 IA Marks : 25 Hrs/Week : 04

Exam Hours : 03 Total Hrs : 52 Exam Marks : 100
PARTA

Actual hour Hours

unit Topics
ofclasses taken

1 VISIBLE ULTRAVIOLET SPECTROPHOTOMETERS:

Electromagnetic radiation, Beer Lambert law, absorption instruments,
7 8
colorimeters, spectrophotometers, sources of error in spectrophotometers,
measurements, calibration.
2 FLAME PHOTOMETERS: Principle of flame photometers, constructional
details of flame photometers, clinical flame photometers, accessories of flame 7 5
photometers, interference in flame photometry and determinations.
3 FLUORIMETERS & PHOSPHORIMETERS: Principle of fluorescence,
measurement of fluorescence, spectrofluorimeters, microprocessor based 6 5
spectrofluorometer, Measurement of phosphorescence.
4 MASS SPECTROMETER & NMR SPECTROMETER: Basic mass
spectrometer, types of mass spectrometer, components of mass spectrometer,
resolution and applications. Principle of NMR, constructional details, 6 7
sensitivity enhancement for analytical NMR spectroscopy, use of computers
with NMR spectrometers.
PART B
5 INFRARED SPECTROPHOTOMETER: Infrared spectroscopy, Basic
components of infrared spectrophotometer, Types of IR spectrophotometer,
7 8
Fourier transform IR spectroscopy. Thermo-analytical Method:
Thermogravimetric analysis (TGA), Differential Thermal analysis (DTA).
6 ELECTROPHORESIS AND DENSITOMETERS: Basic Electrophoresis,
Electrophoresis techniques, paper Electrophoresis, Electrophoresis apparatus,
7 6
spectrodensitometer, microprocessor based densitometer,
microelectrophoresis.
7 CHROMATOGRAPHY: Gas chromatograph basic concepts, parts of gas
chromatograph, Method of peak areas, Liquid chromatography, basic concepts,
6 6
types of liquid chromatography, liquid chromatograph, HPLC principle and
block diagram description.
8 BLOOD GAS ANALYZER: Principle of pH measurement, electrode for pH
measurement, Blood pH measurement, measurement of Blood pCO 2 ,
measurement of Blood pO complete Blood gas analyzer, commercially 6 4
available blood gas analyzers.
TEXT BOOK:
1. Hand Book of Analytical Instruments - By R.S. Khandpur, TMH Publications, 1 Edn. 1989.

REFERENCE BOOKS:
1. Instrumental methods of analysis by H.H Willard, L.L. Meritt & A. Dean, CBS Publications
7 St Edn., 1988
2. Principles of Instrumental analysis by S.J. Holler & T. A. Nilman Saunders college
publications, 5
Edn, 1998.