THE USE OF BENEFICIAL

MICROORGANISMS IN CLEANING
FORMULATIONS
Tony BARTOLINI
Cleaning Solutions Technical Service Manager EEMEA
2

Agenda
Microorganisms overview

Microorganisms and chemistry

What can you do with microorganisms?

Focus on drain application

Regulatory
MICROORGANISMS OVERVIEW
4

MICROORGANISMS ARE LIVING CREATURES

FOUND EVERYWHERE ON EARTH

Only 10% of the

cells in the human
body are human!

Earth Ocean Soil Human Body

5 nonillion 10 million 40 million 40 million in a ml. of saliva
(51030) in a ml. of water in a gram of soil 100 trillion in the gut flora
10% of total dry weight
5

THE VAST MAJORITY OF MICROORGANISMS

ARE EXTREMELY SAFE

In fact, we not only live with microbes,

but our health is dependent on them
(gut bacteria, immune system development, etc)
6

WHAT ARE BACTERIA?

0.5 3m

Bacteria are organisms that are:

Living

Single-celled

Microscopic
7

BACTERIAL NUTRITION/
ENVIRONMENTAL CONDITIONS

Carbon, Nitrogen, Phosphorus and some trace of

minerals to grow

Moisture, proper temperature, appropriate pH and

something to breathe (an electron acceptor like
O2, NO3, etc.)

Production of enzymes (biocatalysts) that break

down compounds like fat, starch and protein to
yield these essential nutrients
8

COMPARISON OF BACTERIA AND ENZYMES

Raw enzymes are...

fast starting biochemicals

specific for a certain reaction
proteins that can be degraded by other enzymes

Bacteria...

take a bit longer awaken and to show activity

produce enzymes on demand, in response to
compounds present (in situ enzyme factories)
can produce suite of enzymes to completely
degrade a compound
can use the components and energy from the
enzymatic action to produce more bacteria
 9

BACTERIA PRODUCE ENZYMES

IN PART, ENZYMES ARE USED TO FEED THE
BACTERIA

Extracellular enzymes

Large molecules,
particulate or
colloidal material
Small, Intracellular
soluble enzymes
compounds

CO2 & H2O

Only ~3% of enzymes used by microorganisms are secreted;

the rest are accessible for useful applications only by using the whole cell
MICROORGANISMS AND
CHEMISTRY
 11

CHEMISTRY/ENZYMES/MICROORGANISMS

SURFACTANT/ ENZYMES MICROORGANISMS

SOLVENT

Cleaning by Cut organic soils Enzyme factory

displacement and /catalyze chemical
solubilization of reaction Metabolize/
organic soils degrade organic
Facilitate the rolling- substrates
Increase the up and cleaning
efficiency of (surfactant, Minimize impact on
enzymes and mechanical action) the environment
microorganisms by
contact improvement

+ H2O
+ CO2

Same organic Same organic Organic content

content content decreased
12

FORMULATE WITH MICROORGANISMS

pH: 5.0 to 9.0 for optimal

stability in liquids

Easy to formulate but need basic expertise (living technology)

SO, WHAT CAN YOU DO WITH
MICROORGANISMS?
14

MICROORGANISMS BENEFITS
Short term cleaning: chemistry and enzymes
Mid-term and long term degradation: microorganisms
Microorganisms
Continuous organic degradation
(fat, starch, protein, VFA)
Chemistry Enzymes

Fight stains Odor control

0 < 1 min 1 hour Time

15

CURRENT CLEANING SOLUTIONS APPLICATIONS

Carpet & fabric Drain Grease Interceptor

Improved cleaning Avoid blockages Odor control
Decrease resoiling Odor control Prevent grease release in
the sewer

Hard surface Odor control Septic & waste

Deep cleaning Drain Odor control
Odor control Hard surface Reduce blockages
Grease interceptor Enhance the degradation
Septic & waste
FOCUS ON DRAIN APPLICATION
17

KITCHEN AND BATHROOM SOIL CLEANING

Effective soil cleaning Before

treatment
with
Novozymes
technology

Novozymes technology effectively

removes both bathroom soil (left)
Water rinse
and kitchen soil (right) applied to only

ceramic tiles.

Treated with
Novozymes
technology
18

KITCHEN SOIL CLEANING

Novozymes technology
effectively removes
kitchen soil (right) while
the control without
treatment (left) shows no
change
19

KITCHEN SOIL CLEANING

Untreated Treated with Novozymes

(kitchen soil only) microorganisms

The tube treated with Novozymes microorganisms

Free flowing drain line (right) shows almost complete soil removal, and the
time for water to flow through the tube improved by 25
seconds.
20

MICROBIAL ACTIVITY ON KITCHEN SOIL

Respirometric activity

The long-lasting,
continuous degradation
(15+ days) of kitchen
soil and grease is
demonstrated by the
respirometric activity of
Novozymes beneficial
microorganisms.
21

PROTEIN DEGRADATION

After 7 days of treatment with Novozymes beneficial microorganisms, a black

protein cube is degraded and black dye is released (right) whereas the control
(left) shows no protein degradation.
22

STARCH DEGRADATION

Complex starch degradation before (left) and 72 hours after (right) addition of
Novozymes beneficial microorganisms.
23

ODOR CONTROL - MICROBIAL GROWTH ON

ODOROUS MOLECULES AND BUILDUP

Respirometry test on VFA

Novozymes beneficial
microorganisms can
degrade both the long-
and the short-chain fatty
acids commonly found in
kitchen drain soil and
responsible for malodors
and buildup.
REGULATORY
25

REGULATORY AND SAFETY INFORMATION

Tight quality control and stringent tests are incorporated

into the production process to ensure that Novozymes
products are manufactured to the highest quality standards
All microorganisms are isolates of naturally occurring
organisms and are not genetically engineered or
modified
All microorganisms have been identified by 16s rDNA
sequencing and confirmed to belong to Risk Group 1 as
defined by the Directive 2000/54/EC.
Safety assessment performed on each Novozymes product
prior to market
Reach compliance of all raw materials is checked

.
26

CONCLUSION

Beneficial bacteria can degrade overtime, making preventive

treatment really efficient

Spore-forming beneficial bacteria have long shelf life

Bacteria are sustainable and less toxic than traditional chemical

control agents

Existing of a large collection of bacteria-based products used

for degradation of grease, oil, odors in kitchen drains and
carpets, organic soiling/stains, etc.

Novozymes beneficial microorganisms could offer safe,

sustainable, efficient solution to any application where control of
undesirable compounds is needed
THANKS FOR
YOUR ATTENTION!
QUESTIONS?