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Refractometry
By:
Bullago, Joshua G.
Caw-is, Imee A.
February 2017
ACKNOWLEDGEMENT
The researchers would like to extend their warmest gratitude to everyone who
To Engr. Genevieve De Vera, for providing deeper understanding of the topic to the
researchers by explaining the theory and principles related to the experiment, as well as the
To the laboratory custodians, for supervising and assisting the researchers in using the
To God, for giving the researchers the strength and patience to perform the
TITLE PAGE i
ACKNOWLEDGEMENT ii
LIST OF TABLES iv
LIST OF FIGURES v
ABSTRACT vi
Chapter 1: INTRODUCTION 1
REFERENCES 11
APPENDICES 12
LIST OF TABLES
INTRODUCTION
The speed of light in a vacuum is always the same, but when light moves through any
other medium it travels more slowly since it is constantly being absorbed and reemitted by
the atoms in the material. The ratio of the speed of light in a vacuum to the speed of light in
another substance is defined as the index of refraction (aka refractive index or n) for the
substance.
Whenever light changes speed as it crosses a boundary from one medium into another
its direction of travel also changes, i.e., it is refracted (Figure 1). (In the special case of the
light traveling perpendicular to the boundary there is no change in direction upon entering the
new medium.) The relationship between light's speed in the two mediums (vA and vB), the
angles of incidence (qA) and refraction (qB) and the refractive indexes of the two mediums
determine its index of refraction. Instead, by measuring the angle of refraction, and knowing
the index of refraction of the layer that is in contact with the sample, it is possible to
determine the refractive index of the sample quite accurately. The refractive index of
different substrates measures with refractometers. There are four main types of
laboratory or Abbe refractometers, and inline process refractometers. There is also the
Rayleigh Refractometer used (typically) for measuring the refractive indices of gases.A
sodium lamp may be used to provide the light source at a known wave-length (589.6 nm)
although many instruments are corrected for daylight use. Nearly all refractometers utilize
this principle, but may differ in their optical design. In the Abbe' refractometer the liquid
sample is sandwiched into a thin layer between an illuminating prism and a refracting prism
(Figure 2). The refracting prism is made of a glass with a high refractive index (e.g., 1.75)
and the refractometer is designed to be used with samples having a refractive index smaller
than that of the refracting prism. A light source is projected through the illuminating prism,
the bottom surface of which is ground (i.e., roughened like a ground-glass joint), so each
point on this surface can be thought of as generating light rays traveling in all directions.
Inspection of Figure 2 shows that light traveling from point A to point B will have the largest
angle of incidence (qi) and hence the largest possible angle of refraction (qr) for that sample.
All other rays of light entering the refracting prism will have smaller qr and hence lie to the
left of point C. Thus, a detector placed on the back side of the refracting prism would show a
Samples with different refractive indexes will produce different angles of refraction
(see Equation 2 above and recall that the angle of incidence and the refractive index of the
prism are fixed) and this will be reflected in a change in the position of the borderline
between the light and dark regions. By appropriately calibrating the scale, the position of the
borderline can be used to determine the refractive index of any sample. In an actual Abbe'
refractometer there is not a detector on the back of the refracting prism, and there are
additional optics, but this is the essential principle. (It is also possible to design a
refractometer based on the reflection of light from the boundary between the prism and the
sample. These types of refractometers are often used for continuous monitoring of industrial
processes.)
In most liquids and solids the speed of light, and hence the index of refraction, varies
causes white light moving through a prism to be refracted into a rainbow. Shorter
wavelengths are normally refracted more than longer ones.) Thus, for the most accurate
measurements it is necessary to use monochromatic light. The most widely used wavelength
of light for refractometry is the sodium D line at 589 nm. If white light were used in the
simple Abbe' refractometer optics shown in Figure 2, dispersion would result in the light and
dark borderline being in different places for different wavelengths of light. The resulting
"fuzziness" of the borderline would make precise work impossible. However, many Abbe'
refractometers are able to operate satisfactorily with white light by introducing a set of
"compensating prisms" into the optical path after the refracting prism. These compensating
prisms are designed so that they can be adjusted to correct (i.e., compensate for) the
dispersion of the sample in such a way that they reproduce the refractive index that would be
since the light is being absorbed and reemitted by the atoms in the sample. Since the density
of a liquid usually decreases with temperature, it is not surprising that the speed of light in a
liquid will normally increase as the temperature increases. Thus, the index of refraction
normally decreases as the temperature increases for a liquid (Table 1). For many organic
liquids the index of refraction decreases by approximately 0.0005 for every 1 °C increase in
water through the refractometer to maintain a given temperature. Most of the refractive index
Refractive index has the large number of applications. It is mostly applied to identify
a particular substance, confirm its purity, or measure its concentration. Generally it is used to
measure the concentration of a solute in an aqueous solution. For a solution of sugar, the
refractive index can be used to determine the sugar content (Brix degree). It can be used also
focusing power of lenses, and the dispersive power of prisms. Also it is applied for estimation
above rows, the study on refractive index of substrates can be useful in various fields (both
refractometer, medicine droppers, small beaker. On the other hand, the following reagents
and materials were used: cotton, ethyl acetate, benzene, chloroform, butanol, sugar solution,
Calibration with distilled water was first done by the laboratory supervisor. Distilled
water using glass dropper was placed on the prism surface of the refractometer. It remained to
stand for at least three to five minutes for temperature stability. The turn mode selector was
shifted to refractive index option. The eyepiece is then focused until crosshair is clear. The
adjustment control was rotated counterclockwise so that the shadow line an crosshair meet.
The dispersion correction wheel is also rotated until a possible color free shadow line is
produced. Then the shadowline is adjusted to meet the crosshair. The red button and
To calibrate further, the mode selector is turned to BX-TC position. The “read” display
button was pressed. An LED reading of 000.0 or 0.1 is normal. A negative sign and decimal
point however will indicate no reading. If this persists, the adjustment control unit must bte
turned until a normal reading can be displayed. Only slight adjustment is required. Reading
through the reticle crosshair to shadowline, should measure 000.0. The process is repeated
Determining the refractive index of the substance has now been made possible. The
prism assemble cover is then opened and the protective lens tissue removed. The measuring
prism surface is cleaned with cotton and alcohol before any sample has been placed. The
mode selector is put to desired position. Adjustment control was turned counterclockwise to
position the shadowline at bottom of field of view. The shadowline is in center and is in
center to crosshair for accurate reading. The eyepiece is also adjusted to focused on crosshair.
The shadowline is moved to crosshair reticle through coarse adjustment knob. The dispersion
correction wheel has been rotated to eliminate any red or green color at edge of shadownline.
After such conditions are met, the molar refraction was calculated.
Proper laboratory guidelines were followed on disposing some excess samples and on
handling the excess unknown liquid. The sets of apparatus were cleaned thoroughly with
A. RESULTS
B. DISCUSSION
The Abbe Refractometer used was very precise, accurate and fast in acquiring the
respective indexes of refraction of the solutions to be analyzed. Using the equipment, the
values needed for the computation for the molar refraction was easily taken. When adjusting
the eyepiece, there is a dependence on the clarity and focus of the sample with the eyes of the
person manipulating the refractometer because what is clear to one person may not on the
other. When adjusting the dispersion correction wheel, there is a great deal to make sure the
crosshair is adjusted properly. The refractive index of a liquid varies with temperature that is
why there is a must for the researchers to make use of the proper density of the liquid to be
analyzed in the proper temperature scale. These values were calculated given the constants
given in the Perry’s Chemical Engineers’ Handbook. The molar mass values were also taken
in the handbook.
The molar refraction was then computed given the formula and the following solution
through a given substance. The amount by which the light is refracted determines the
refractive index. Refractive index can be used to identify an unknown liquid compound.
Refractive index is defined as the ratio of the velocity of light in air to the velocity of light in
the medium being measured. In the experiment the refractometer used was able to determine
the index of refraction of Chloroform, Benzene, Ethyl acetate, Sugar solution, Butanol, and
Carbon tetrachloride as well as its temperature. The mode in the refractometer was already
settled and was ready to use. The sample solution was placed to the prism using a dropper
and the prism was closed. Adjustments in both the dispersion correction wheel and the
eyepiece was needed primary to the control knob before reading the output value. Since the
index of refraction depends on the temperature and the pressure the density at a given
temperature was used to correct and achieve the molar refraction of the samples. Combining
refractive index and substance density it is possible to define a quantity that is temperature
independent. It is called specific refraction and multiplying it with the molar mass gave the
molar refraction results in the experiment. The experiment enabled the researchers to
successfully manipulate and operate the refractometer as well as determining the molar
refractometry.
To limit the errors in determining the molar refraction by means of the refractometer,
the following should be observed; the prism must be wiped properly and cleaned with alcohol
followed by distilled water to avoid contaminants in the sample, do not wipe the prism dry to
avoid any residue in the prism that can affect the accuracy of the reading, when rotating the
eyepiece to focus the crosshair, one person can see the crosshair more clearly than another
because of human error and visible clarity and it is suggested that only one should operate per
liquid, and the shadow line must also be made sure to be below the field of view. When
proper care and correct procedure is followed and exercised using the refractometer, this
equipment can be used for highly accurate and precise determination of the refractive index
of materials that can help to determine the specific refraction and molar refraction of a
sample.
REFERENCES
Abbe Refractometer - is a highly reliable instrument with high precision that is used to
Medicine Dropper- a laboratory apparatus used to transfer samples through suction of the
Small Beaker- a laboratory apparatus used mainly as containers for the liquid samples and
cleaning agent
APPENDIX B
Definition of Terms
Refraction- is the bending of a wave when it enters a medium where its speed is different.
Refractometry- is a technique that measures how light is refracted when it passes through a
given substance.
Refractometer- is an instrument that measures the extent to which light is bent, or refracted,
when it moves from air into a sample and is typically used to determine the index of
refraction of a sample.
Refractive index- also known as the index of refraction, this is defined as the speed of light in
Molar Refraction- indicates the manner in which a molecule interacts with light. This is a
Computations
(𝑛2 − 1)𝑀
𝑅𝑚 =
(𝑛2 + 2)𝜌
For Chloroform:
𝑔
(1.44392 − 1)(119.378 ) 𝑐𝑚3
𝑅𝑚 = 𝑚𝑜𝑙 = 21.8130
𝑔 𝑚𝑜𝑙
(1.44392 + 2)(1.4968 3 )
𝑐𝑚
𝑔
(1.37182 − 1)(88.105 ) 𝑐𝑚3
𝑅𝑚 = 𝑚𝑜𝑙 = 22.2411
𝑔 𝑚𝑜𝑙
(1.37182 + 2)(0.8999 )
𝑐𝑚3
For Butanol:
𝑔
(1.39802 − 1)(74.122 ) 𝑐𝑚3
𝑅𝑚 = 𝑚𝑜𝑙
𝑔 = 22.1158 𝑚𝑜𝑙
(1.39802 + 2)(0.8089 )
𝑐𝑚3
For Benzene:
𝑔
(1.49912 − 1)(78.112 ) 𝑐𝑚3
𝑅𝑚 = 𝑚𝑜𝑙 = 26.1622
𝑔 𝑚𝑜𝑙
(1.49912 + 2)(0.8768 )
𝑐𝑚3
𝑔
(1.33672 − 1)(342 ) 3
𝑅𝑚 = 𝑚𝑜𝑙 = 69.6632 𝑐𝑚
𝑔 𝑚𝑜𝑙
(1.33672 + 2)(1.0200 )
𝑐𝑚3
𝑔
(1.45812 − 1)(153.82 ) 𝑐𝑚3
𝑅𝑚 = 𝑚𝑜𝑙 = 26.4022
𝑔 𝑚𝑜𝑙
(1.45812 + 2)(1.59 )
𝑐𝑚3
APPENDIX D
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