Name: John Michael F.

Ruiz

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results

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1. Which of the following is/are true of bile?

Correct answer: b. It works by a physical process.

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2. The substrate used in this simulation is

Correct answer: c. vegetable oil.

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3. When fatty acids are liberated by lipase, the

pH Correct answer: b. decreases.

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4. One of the products of the chemical digestion of lipids is

Correct answer: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
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Stop & Think Questions:

What does the pH measure?
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Correct answer: d. both lipase activity and fatty acid release

Why is it difficult to detect whether lipase is active in tube 5?

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Correct answer: d. The pH is already very low, so a decrease in pH might be difficult to detect.

Experiment Data:

Tube No. Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time Temp. pH

1 Lipase Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 6.21
2 Lipase Vegetable Oil Deionized pH 7.0 Buffer 60 37 7.00
Water
3 Lipase Deionized Water Bile Salts pH 9.0 Buffer 60 37 9.00
4 Deionized Water Vegetable Oil Bile Salts pH 7.0 Buffer 60 37 7.00
5 Lipase Vegetable Oil Bile Salts pH 2.0 Buffer 60 37 2.00
6 Lipase Vegetable Oil Bile Salts pH 9.0 Buffer 60 37 9.00

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Post-lab Quiz Results
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1. What is the product of lipase hydrolysis?

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Correct answer: d. fatty acids

2. From your results, which pH is ideal for pancreatic lipase digestion?

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Correct answer: b. pH 7.0

3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
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Correct answer: c. tube 4

4. From your results, where (in theory) would pancreatic lipase be

active? You did not answer this question.

Correct answer: d. mouth and pancreas

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Review Sheet Results
1. Explain why you can't fully test the lipase activity in tube 5.
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2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
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3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
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4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
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