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GROUP 7A
ANCHETA, Celline Victoria
GERODIAS, Mark Patrick
PANOPIO, Allen Jeremy
REYES, Dana Gillian
RUIZ, John Michael
TABIRAO, Mia Veronica
VELASCO, Patrick Benedict
VITUG, Jasmine Lorraine
Questions:
1. Describe the effect that boiling had on the activity of amylase. Why did boiling
have this effect? How does the effect of freezing differ from the effect of boiling?
- Appropriate temperature is highly needed for enzyme to work
optimally, in this case, amylase works at 37 degrees Celsius. When the
temperature increases like in boiling, the amylase’s activity is inhibited
due to the denaturation. On the other hand, freezing does not change
the structure of the enzyme thus, it will not affect its activity.
2. What is the purpose for including tube 3 and what can you conclude from the
result.
- The purpose of the tube 3 is to serve as a basis or a standard and for
comparison between tubes 1 and 2 that was subjected to boiling and
freezing, respectively with the same incubation time and pH level (7.0).
4. From what you learned in this activity, suggest a reason why salivary amylase
would be much less active in the stomach.
- Salivary amylase would be much less active in the stomach because of
its pH level. The stomach is too acidic for this enzyme work optimally.
The pH should approximately at 7.0.
2. Can you determine if fat hydrolysis occurred in tube 5? Why or why not?
- No, because the pH of the solution is too acidic for the enzyme.
3. Would pancreatic lipase be active in the mouth? Why or Why not?
- Yes, because pancreatic lipase works optimally at pH 7.0 and the
mouth has a pH closer to 7.0.
4. Describe the physical separation of fats by bile salts.
- Bile salts physically emulsifies fat globules (triglyceride clumps) by
separating it into fat (triglyceride) droplets.