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Protein Purification: Principles and Practice (Springer Advanced Texts in Chemistry) Softcover reprint of
Protein Purification:
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New textbooks at all levels of chemistry appear with great regularity. Some fields such as basic biochemistry, organic
re action mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or
ISBN-13: 978-1441928337
revised editions are published sufficiently often to keep up with progress in research. However, some areas of
ISBN-10: 1441928332
Why is ISBN important? chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The
most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written
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then seek out and persuade experts in these fields to produce relatively concise but instruc tive introductions to their
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This item: Protein Purification: Principles and Practice (Springer Advanced Texts in Chemistry) by Robert K. Scopes Paperback $119.99
Lehninger Principles of Biochemistry by David L. Nelson Hardcover $221.00

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Editorial Reviews
Review

From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly"
(American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)

Product details
Series: Springer Advanced Texts in Chemistry
Paperback: 380 pages
Publisher: Springer; Softcover reprint of hardcover 3rd ed. 1994 edition (December 6, 2010)
Language: English
ISBN-10: 1441928332
ISBN-13: 978-1441928337
Product Dimensions: 6 x 0.9 x 9 inches
Shipping Weight: 1.5 pounds (View shipping rates and policies)
Average Customer Review: 6 customer reviews
Amazon Best Sellers Rank: #3,100,523 in Books (See Top 100 in Books)
#1183 in Books > Medical Books > Basic Sciences > Cell Biology
#2298 in Books > Science & Math > Biological Sciences > Biology > Molecular Biology
#3309 in Books > Engineering & Transportation > Engineering > Bioengineering > Biochemistry

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maneki neko

Lots of good information

July 25, 2011
Format: Hardcover Verified Purchase

I am a graduate student that was given the task to purify a protein of interest. We have been trying to purify this protein for a while however since
we are using recombinant technology in E. coli it's being expressed as an inclusion body. I received the second edition which was published in the late
eighties before recombinant expression was widely used so it's a topic that isn't really touched in the text (although the author anticipated its wide
usage in the future).

This book does have a great deal of information on the basic protocol of protein purification as well as some handy tips to use in the event that your
protein is insoluble or not easily purified. I do recommend this book for people who are just starting to tackle protein purification. It is well written
and the author clearly knows what he is talking about. However if you are using this to optimize purification of recombinant proteins I would
recommend looking for a newer book (and if you find a better one comment back!)

Comment 3 people found this helpful. Was this review helpful to you? Yes No Report abuse

Averroes

should be on the book shelf of everyone interested in the field of protein purfication.
April 26, 2007
Format: Hardcover Verified Purchase

I find this book, protein purification: principles and practice, is one of the best sources to understand the bases of protein purification techniques.
The book describes the mechanisms, chemistry bases, and practical considerations of different protein purification methods. Moreover, the book is
written easily for scientists/technicians with background ranging from weak to excellent in biochemistry. If you were new to the field of protein
purification, this book will help you to build skills in a very short time since it fills the missing gapes in almost famous protocols by providing very
easy background information about each purification procedure. I highly recommend to have it on your book shelf.

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Brian Barron

Great Reference for Protein Purification

May 25, 2013
Format: Hardcover Verified Purchase

This book is great. It provides good, detailed biochemical explanations / theory for a variety of techniques used in protein purification, which are
essential for troubleshooting tricky purifications or just getting more knowledgeable in the field.

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Celia Baula

Very informative
February 16, 2011
Format: Hardcover Verified Purchase
Working on various biochemical techniques but I have not used them for a while. This is a great refresher and intro for beginners.

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A customer

The best book for understanding protein purification!

August 21, 1996
Format: Hardcover
Scientists and educators alike will benefit from Robert K. Scopes
thorough and up-to-date examination of the techniques behind the science
of protein purification. Written with enough simplicity and easy
reading for the upper-level undergraduate student, but with all the
facts and formulas for hard-core theorists, Protein Purification may
well be the most concise and easy to read book of its kind on the
textbook market. A must read for those interested in any form of
protein science, anyone from the industrial biochemist to the graduate
student will benefit from Robert's full description of the most current
procedures for separating proteins by size, charge, or physical
characteristics. An in-depth discussion of procedures used from the
beginning of scientists' knowledge of the individual characteristics
of protein charge and size to the most current procedures involving dye
ligand chromatography will ensure an overall knowledge of the field.
Robert's belief that a ninety percent separation of protein is
theRead more standard for any reliable purification procedure is augmented
minimun

Comment 10 people found this helpful. Was this review helpful to you? Yes No Report abuse

Gerald J. Nora

A Clear, In-depth Treatment of Biochemistry

August 31, 2001
Format: Hardcover
In the first chapter of Protein Purification, Scopes notes that more and more biochemical techniques are black boxed, with kits eliminating the need
for an understanding of biochemical principles (e.g., "precipitate with Acme Solution B, pour off Solution B, and redissolve precipitant in Solution C").
Scopes notes that with a proper understanding of biochemistry, one can save money on kits and do more purification steps with standard lab
materials and reagents. He wrote this book to help biochemistry labs make more informed decisions on protein purification rather than just
defaulting on some already familiar kit or protocol.
Scopes succeeded in this purpose with flying colors: he hit a rare balance that makes this book fun to read. Each chapter is well-laid out, concetrating
on a single separation technique's general practice and the theoretical foundations for that method. The basic ideas and materials are laid out, with
clear, well-picked illustrations.
I was particularly happy with the mathematical treament Scopes gives: understanding the thermodynamics or physics of a process aids your intuitive
understanding and makes your troubleshooting skills much more honed.

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