Exam  1  MIBO3500  Spring  2014

Exam 1 MIB03500 Spring 2014

(40 pts possible on exam - your percentage is recorded in eLC New as your grade on the exam.)
(40  pts  possible  on  exam  -­‐  your  percentage  is  recorded  in  eLC  New  as  your  grade  on  the  exam.)

M I NAME: 8 1 0 ft:
FULL  NAME:  __________________________________________  810  #:  _____________________

Short Answer Section: Place your answers in the space provided below. PRINT your answers legibly and DO
Short  Answer  Section:  Place  your  answers  in  the  space  provided  below.  PRINT  your  answers  legibly  and  DO
NOT EXCEED the sentence limits for each question. Wrong information will be counted against you. (15 pts
NOT  EXCEED  the  sentence  limits  for  each  question.  Wrong  information  will  be  counted  against  you.  (15  pts
total for the section)
total  for  the  section)
26.  
26.
a. PPut  the  above  items  in  order  from  smallest  to  largest:  (1  pt  –  no  partial  credit  available)
u t the above items in order from smallest to largest: (1 pt — no partial credit available)
Smallest:
Smallest: _Amino Acid
_Amino  Acid________________
_Ribosome_________________
_Ribosome
_Virus
_Virus_____________________
i _E.  coli____________________
_E. coil
Largest:
Largest: _Red  Blood  Cell_____________
_Red Blood Cell

b.
b. L List  which  of  these  are  living  organisms.  (0.5  pt)
i s t which of these are living organisms. (0.5 pt)

E. coil or Red Blood Cell

E.  coli  or  Red  Blood  Cell

27.  
a.
a. I sIs  this  a  defined  medium  or  a  complex  medium?  (1  pt)
this a defined medium or a complex medium? (1 pt)
Complex

b. Explain  why  you  chose  this  answer  for  27a.  Limit:  2  sentences.  (1  pt)
b. Explain why you chose this answer for 27a. Limit: 2 sentences. (1 pt)
It  contains  yeast  extract  which  contains  unknown/unmeasured  chemical  constituents  making  it
It contains yeast extract which contains unknown/unmeasured chemical constituents making it
impossible  to  be  a  defined  medium
impossible to be a defined medium

c.
C. I Is  this  media  selective,  differential,  neither,  or  both?  (1  pt)
s this media selective, differential, neither, or both? (1 pt)
Both selective & differential
Both  selective  &  differential

d. Explain  why  you  chose  this  answer  for  27c.  Limit:  2  sentences.  (1  pt)
d. Explain why you chose this answer for 27c. Limit: 2 sentences. (1 pt)
It  is  differential  because  two  phenotypes  can  be  observed:  pink  (ferments  sorbitol)  and  white  (no
It is differential because two phenotypes can be observed: pink (ferments sorbitol) and white (no
sorbitol  fermentation).
sorbitol fermentation).
It  is  selective  because  it  contains  bile  salts  (toxic  to  Gram  +)  and  prevents  growth  of  Gram  +  cells
It is selective because it contains bile salts (toxic to Gram +) and prevents growth of Gram + cells

28. Recall  the  formula  (N

28. Recall the formula (IN,t  =  N
n  
= N0  x  2 ).  Show  your  work  (e.g.,  formula  setup/calculation  of  generations)  or  no
o xcredit  will  be  given  for  your  answer.  (2  pts)
2 ' will
credit ) . be given for your answer. (2 pts)
S N N0=5 w
h o
y oo u r
w Growth Time =
k 150 minutes
Growth  Time  =  150  minutes
= o r
( 5 e . g .
, 150  minutes  /  10  minute  generation  =  15  generations
150 minutes / 10 minute generation = 15 generations
f o r 15   m u
N
Nt=(5)(2) =  (5)(32,768)  =  163,840  cells
l a
s te t u p /
c =a l c u l a
t i( o
n 5
o ) 11
f (
 Exam  1  MIBO3500  Spring  2014
Exam 1 MI1303500 Spring 2014
(40 pts possible on exam - your percentage is recorded in eLC New as your grade on the exam.)
(40  pts  possible  on  exam  -­‐  your  percentage  is  recorded  in  eLC  New  as  your  grade  on  the  exam.)

FOR
FOR
SCRATCH
SCRATCH
WORK
WORK

22
 Exam  1  MIBO3500  Spring  2014
Exam 1 MI1303500 Spring 2014
(40 pts possible on exam - your percentage is recorded in eLC New as your grade on the exam.)
(40  pts  possible  on  exam  -­‐  your  percentage  is  recorded  in  eLC  New  as  your  grade  on  the  exam.)

FULL NAME: 8 1 0 #:
FULL  NAME:  __________________________________________  810  #:  _____________________
29.
29.
a. WWhat  phase  of  the  microbial  growth  curve  is  represented  by  letter  B?  (0.5  pt)
h a t phase of the microbial growth curve is represented by letter B? (0.5 pt)
Exponential (also known as logarithmic) phase
Exponential  (also  known  as  logarithmic)  phase

b.
b. I In  which  section(s)  of  the  graph  are  cells  undergoing  DNA  replication?  (0.5  pt)
n which section(s) of the graph are cells undergoing DNA replication? (0.5 pt)
A,  B,  C,  and  D  (Not  all  cells,  all  the  time  -­‐  some  are  dying  -­‐  but  there  are  cells  replicating  DNA  at  every
A, B, C, and D (Not all cells, all the time - some are dying - but there are cells replicating DNA at every
phase)
phase)
c. I nIn  which  section(s)  of  the  graph  are  cells  undergoing  binary  fission?  (0.5  pt)
which section(s) of the graph are cells undergoing binary fission? (0.5 pt)
B,  C,  and  D  (See  note  from  part  B  about  dying  cells)
B, C, and D (See note from part B about dying cells)

d. I In  which  section(s)  of  the  graph  are  cells  producing  starvation  proteins?  (0.5  pt)
n which section(s) of the graph are cells producing starvation proteins? (0.5 pt)
C  and  D  (No  need  to  express  these  proteins  until  some  growth  limitation  is  reached  -­‐  status  C)
C and D (No need to express these proteins until some growth limitation is reached - status C)

30.
30.  
a.
a. WWhat  major  catabolic  pathway  is  this  organism  using  to  generate  ATP:    fermentation,  aerobic
h a t major catabolic pathway is this organism using to generate ATP: fermentation, aerobic
respiration  or  anaerobic  respiration?  (1  pt)
respiration or anaerobic respiration? (1 pt)
Fermentation
Fermentation
b. Explain  how  you  deduced  the  answer  to  30  a.  Limit:  3  sentences.  (1.5  pts)
b. Explain how you deduced the answer to 30 a. Limit: 3 sentences. (1.5 pts)
Any  of  the  following  is  sufficient:
Any of the following is sufficient:
1.  pH  change  to  acidic  environment  indicates  acidic  end  products  &  fermentation
1. pH change to acidic environment indicates acidic end products & fermentation
2.  Presence  of  3–4  carbon  compounds  (not  only  CO
2. Presence of 3-4 carbon compounds (not only CO2)  indicates  fermentation
2
3.  Neither  O
3. Neither 0 2  or  inorganic  primary  electron  acceptors  change  in  concentration
) i n d i c a t e s
2 o r
c. Assume  the  organism  can  only  follow  the  pathway  you  deduced  in  30a.  Calculate  how  much  net  ATP
C. Assume
f e r m the eorganism
n t a cant ionly
o follow
n the pathway you deduced in 30a. Calculate how much net ATP
i n o r g
the  organism  is  produces  per  cell  via  substrate-­‐level  phosphorylation  if  a  single  cell  has  4  molecules
the organism is produces per cell via substrate-level phosphorylation if a single cell has 4 molecules
a n i c
of  glucose.  Show  your  work  (e.g.,  conversions  from  NADH/FADH
pof glucose.
r i Show your work (e.g., conversions from NADH/FADH2)  or  no  credit  will  be  given  for  your
answer.  (1.5  pts)
m2 a (1.5
answer. r pts)
)SLPo inr EMnpathway
o conly
r ewhich
d inets
t 2 ATP w per
i glucose
l l molecule
SLP  in  EM  pathway  only  which  nets  2  ATP  per  glucose  molecule
y
eb l e g i v e n
therefore,  2  x  4  glucose  =  8  ATP
therefore,
f o 2 x 4 glucose
r = 8 ATP y o u r
e c
t r
o
d.
d. AAssume  the  organism  can  only  follow  the  pathway  you  deduced  in  30a.  Calculate  how  much  net  ATP
s s u m e the organism can only follow the pathway you deduced in 30a. Calculate how much net ATP
n
the  organism  is  producing  per  cell  via  oxidative-­‐level  phosphorylation  if  the  cell  has  4  molecules  of
athe organism
c is producing per cell via oxidative-level phosphorylation if the cell has 4 molecules of
glucose.  Show  your  work  (e.g.,  conversions  from  NADH/FADH
e Show your work (e.g., conversions from NADH/FADH2)  or  no  credit  will  be  given  for  your
cglucose.
answer.  (1.5  pts)
p2
answer.t (1.5 pts)
o) o rr n do
o not cutilize
r e OLP
d isot there w i bel a 0l ATP yield per glucose molecule
Fermenters  do  not  utilize  OLP  so  there  would  be  a  0  ATP  yield  per  glucose  molecule
Fermenters would
sb e g i v e n
cf
therefore o 0 x 4 =r0 ATP
therefore  0  x  4  =  0  ATP
hy o u r
a
n
g
e
i
n 33
c o
 Exam  1  MIBO3500  Spring  2014
Exam 1 MI1303500 Spring 2014
(40 pts possible on exam - your percentage is recorded in eLC New as your grade on the exam.)
(40  pts  possible  on  exam  -­‐  your  percentage  is  recorded  in  eLC  New  as  your  grade  on  the  exam.)

FOR
FOR
SCRATCH
SCRATCH
WORK
WORK

44