Materials and Methodology Outline

4.1 Materials

4.1.1 Ulva Lactuca

.1.2 Reagents and Chemicals ( gagamiting chemicals para sa pretreament at analysis)

4.2 Experimental Set-Up

4.2.1 Experimental Flowchart

4.2.2Equipments used

4.3 Preparation of Material

4.3.1 Pretreatment of Ulva

4.3.1.1 Acid Hydrolysis

4.3.1 2 SWE

4.4 Experimental Procedure

4.4.1 Saccharification

4.4.2 Fermentation

4.5 Analysis

4.5.1 HPLC

4.5.2 DLS

4.6 Ethical, Environmental, Safety and Health Concern

4.6.1 Safety

4.6.1.1 Materials Safety

4.6.1.2 Hazard

4.6.2 Waste disposal (acid, alage and isip pa)

4.6.3 Other ethical concern

4.6.1 (sabhin lang na wala tayong ginamit na people, animals and wildlife)
 Flowchart

Biomass Ethanol

Simultaneous Saccharification and Fermentation (SSF)

Pretreatment Acid Hydrolysis Fermentation Distillation and

(Subcritical (Saccharification) (Conversion of sugar Evaporation
Water) to ethanol)

Waste
Filter/Washing
Management Lignin

Residue
List of Materials

1. Ulva lactuca (Freeze dried mass)

2. Sulfuric Acid (Different concentrations)
3. Zymomonas mobilis culture