Micro Lab Final Flashcards

2/5/2017 Micro Lab Final Flashcards
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Title
Micro Lab Final
Description
blah.
Total Cards
160
Subject
Microbiology
Level
Undergraduate 2
Created
05/07/2011
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Additional Microbiology Flashcards
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Term
Bacillus subtilis
Definition
gram positive. bacillirod. produces amylase. forms endospores. strict aerobicrequires oxygen to grow. colonies with a raised and a dull
surface.
Term
Enterobacter aerogenes
http://www.flashcardmachine.com/microlabfinal3.html 1/27
Definition
gram negative. bacilli rods. single. fat> plump. in gramstain dark red. oxidase negative. large intestine, E. coli.
Term
Escherichia coli
Definition
gram negative. bacillirods. facultative anaerobe. single. bipolar staining with methylene blue. in gram staining colonies are light red.
opportunistic pathogen. oxidase negative. does not produce amylase. could produce catylase.
Term
Proteus vulgaris
Definition
gram negative. bacilli rod.
Term
Serratia marcescens
Definition
gramnegative. bacilli rod. colonies are orange/red when grown at low temperatures. large, irregularly edged.
Term
Pseudomonas aeruginosa
Definition
gram negative. bacillirod. obligate anaerobe. grown in endo agar. produce catylase.
Term
Saccharomyces cerevisiae
Definition
gramnegative. fungi. dimorphic. coccioval/elongated shape. reproduces by budding which you will most likely see asexual, similar to
binary fission but gets to unequal cells budding yeast.
Term
Mycobacterium tuberculosis
Definition
red, acidbase stain. bacillirods. tend to clump together. grows as a very hard, granular colonnade. must include background color even if
it's colorless. nonmotile, nonsporing, weakly grampositive.
Term
Micrococcus luteus
Definition
grampositive cocci (round). Anaerobe. Colonies are canary yellow. Tetrade. Rodlike.
Term
Mycobacterium spegmatis
Definition
grampositive (bacillus). colonies are slow growing and punctiform. has waxy cell walls so it's generally gramnonreactive. use acid fast
staining
Term
Staphylococcus epidermidis
Definition
gram positive sphere cocci. facultative anaerobe. cluster/netlike. colonies are white, raised, circular, and entire. opportunistic pathogen.
with gramstaining colonies are purple. normally lives on skin.
Term
Clostridium sporogenes
Definition
sporeforming, grampositive, anaerobe, bacillirod. colonies are irregular and rhizoid. viewed with transmitted light. forms endospores.
obligate anaerobe.
Term
Streptococcus faecalis
Definition
grampositive. aerobe tolerant. lactic acid producing bacteria. does not produce catylase.
Term
ENDO agar
Definition
media used to detect water contamination. contains color indicators that are grampositive inhibitors. detects lactose fermentors>
selective and differential, for gram negative.
Term
Eosin Methylene Blue Agar
Definition
media used for the isolation of fecal coliforms> selective and differential, for gram negative
Term
tryptic soy agar
Definition
all purpose media
Term
lauryl tryptose broth
Definition
media used in the presumptive test for the presence of coliforms. lactose containing medium. bacteria, if aerogenic (gas produce) in this
broth, are presumed coliforms.
Term
fluid thioglycollate medium
Definition
media that tests aerotolerance of bacteria. appropriate for a broad variety of aerobic and anaerobic organisms. oxidized portion aerobic
turns pink. reduced portion anaerobic remains colorless.
Term
starch agar
Definition
media used to show how amylase breaks down the starch (using iodine)
Term
brilliant green lactose bile broth
Definition
media used in confirmed test of gas production in the water analysis. lactose broth medium. function: selective for gram negative> has
lots of sugar, shown if has turbidity, MUST use streak plate isolation. ALSO CAN USE TO DETERMINE IF LAURLY TRYPTOSE BROTH
CONTAINS COLIFORMS.
Term
macconkey's agar
Definition
media that inhibits gram positive microbes. selective and differential medium containing lactose, bile salts, neutral red, and crystal violet.
*used to isolate members of enterbacteriaceae. DIP N COUNT!
Term
triple sugar iron agar
Definition
differential agar in which bacteria that form hydrogen sulfide will blacken the agar
Term
media
Definition
nutrient solutions
Term
solid media
Definition
used for separation of bacteria and letting them grow in isolated colonies or for maintaining stock culture
Term
liquefiable solid media
Definition
colloidal suspension of nutrient material which can be liquefied with heat and poured as a liquid, or when allowed to cool, the medium can
be used in a solid state.
Term
semisolid media
Definition
see if bacteria is motile or not. *used for medical specimens containing bacteria
Term
liquid media
Definition
used for cultivation of large quality of microbes
Term
allpurpose media
Definition
supports growth of most bacteria
Term
differential media
Definition
media in which biochemical products of some chemicals react with, creating a visible result in the colony or media
Term
selective media
Definition
inhibit the growth of some microbes
Term
enrichment media
Definition
contains nutrients that favor growth of certain organisms
Term
synthetic media
Definition
media in which the exact ion concentration of every chemical in the medium is known
Term
nondefined media/chemically complexed
Definition
media contained natural ingredients such as beef extract (*these are the type that we made in the lab)
Term
dehydrated media
Definition
standardized media prepared in large batches, desiccated, bottled, and sold. *all bacteria must have water, carbon, an energy source,
nitrogen, and minerals
Term
agar media
Definition
solidifying agent used to physically, not nutritionally, support bacterial growth.
Term
autoclave
Definition
a sterilizer employing superheated steam under pressure
Term
holding temperature
Definition
temperature needed for media to be poured
Term
pure culture
Definition
contains only one kind of bacteria
Term
contamination
Definition
introduction of unwanted organisms
Term
aseptic/sterile techniques
Definition
procedure that both protects culture and protects the environment. *used to transfer bacteria so that the bacteria can have nutrients to
grow on and escape their own toxic waste
Term
colony
Definition
the descendants of one cell growing together on a solid surface
Term
flaming
Definition
used to incinerate any organisms on the inoculating wire
Term
pellicle
Definition
suface membrane produced by organisms cultivated in broth that float on the top of the medium
Term
sediment
Definition
organisms that sink to the bottom
Term
uniform fine turbidity
Definition
uniformed cloudiness
Term
flocculent
Definition
clumping of organisms in broth
Term
aerotolerance
Definition
ability/inability to grow the presence of oxygen
Term
obligate aerobes
Definition
need oxygen seen at the top of broth
Term
facultative aerobes
Definition
grow in presence/absence of oxygen
Term
aerotolerant aerobes
Definition
respire anaerobically even when oxygen is present
Term
anaerobes
Definition
doesn't matter live throughout the media
Term
microaerophiles
Definition
survive only in environments containing lower than atmospheric levels of oxygen grow in the middle
Term
obligate anaerobes
Definition
cannot grow with oxygen inhibit the lower 2/3 of media
Term
microscope
Definition
device for magnifying objects that are too small to be seen with the naked eye
Term
simple microscope
Definition
onelens magnifier
Term
compound microscope
Definition
microscopes that employ to or more sets of lenses
Term
total magnification
Definition
multiply the objective lens times the ocular lens
Term
working distance
Definition
the distance separating a specimen and your objective lens
Term
condenser
Definition
controls quality of light on microscope concentrates the light and makes illumination of the specimen more uniform
Term
microscopic field
Definition
the area of the microscope slide that you see through a lens
Term
resolving power
Definition
ability to distinguish closetogether objects, resolution, and clarity
Term
parfocal
Definition
lenses are adjusted so that the specimen remains almost in focus after the microscope is rotated the nosepiece to utilize a different
objective lens
Term
oil immersion steps
Definition
high power, in focus drop a drop of oil directly on smear, then rotate to oil immersion lens
Term
objective lens then ocular lens
Definition
magnified first by ____ to produce real image and then _____ to produce a virtual image.
Term
photosynthetic
Definition
able to make cellular molecule use light energy and CO2
Term
chemosynthetic
Definition
able to construct cellular molecules using preformed organic molecules for carbon and energy
Term
saprophytic
Definition
living in dead/decaying organic material
Term
aerotaxis
Definition
the ability to move toward or away from chemicals, light, or oxygen
Term
stained preparations
Definition
the preparation applied to dead cells
Term
unstained preparations
Definition
preparation applied to living organisms prepared by wet mount (prepared by placing a drop of liquid containing specimens onto a glass
slide and covering it. **purpose: to determine motility) or hangingdrop (specimen hangs suspended from a coverslip into the indentation
of a glass depression slide. **benefit: allows longer observation)
Term
brownian movement
Definition
a random bouncing that results from microbes colliding with molecules that are in constant motion with the liquid environment. **differs
from motility, which is directed movement
Term
prokaryotic cells
Definition
tiny and lack a membranebound nucleus
Term
cocci
Definition
circles/spheres
Term
rods
Definition
lines
Term
spirilla
Definition
spiral
Term
vibrios
Definition
slightly curved rods
Term
coccobacilli
Definition
short rods
Term
spirochetes
Definition
flexible spirals
Term
flagella
Definition
threadlike organelles that propel organisms through liquid
Term
refractile
Definition
shiny
Term
endospores
Definition
tough, resting structures
Term
conidia
Definition
reproductive spores formed at the tips of branching filaments
Term
taxis
Definition
movement of motile organisms that are positive or negative (e.g. chemotaxis, phototaxis, aerotaxis)
Term
eukaryotic cells
Definition
mostly larger than prokaryotic cells and usually contain a visible, membranebound nucleus
Term
protozoa

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2/5/2017 Micro Lab Final Flashcards

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