UNIVERSIDAD NACIONAL DE INGENIERÍA

FACULTAD DE INGENIERÍA QUÍMICA Y TEXTIL

ÁREA ACADÉMICA DE INGENIERÍA QUÍMICA
PI722/A – Procesos Bioquimicos
PERIODO ACADEMICO 2018-1 / FECHA: 28/04/2018

Practica Calificada 2

 La práctica consta de 2 Temas que contienen en total 2 preguntas para responder que acumula 20 puntos
 La prueba es individual y está prohbido el uso de apuntes de cualqueir tipo. En caso contrario, la prueba es
inválida y ocasionará su anulación y calificación con la Nota A0 no sustituible (art.23 RR N°964).

Temas 2 y 3: Bioreactores y Cineticas Biotecnológicas

1. Cinetica de producto: Se desea fermentar glucosa a etanol en un reactor batch usando

Saccharomyces cerevisiae. Graficar el comportamiento de las concentraciones de las células,
sustrato, producto y crecimiento en función del tiempo. La concentración inicial de las células
es de 1 g/dm3 y la concentración incial de sustrato es de 250 g/dm3.

Datos:
Cp* = 93 g/dm3 YC/S = 0.08 g/g
n = 0.52 YP/S = 0.45 g/g
M = 0.33 h-1 YP/C = 5.6 g/g
KS = 1.7 g/dm3 Kd = 0.01 h-1
m = 0.03 g sustrato/g células ∙h

Graficar y analizar comportamiento de:

a) x, s, p vs t
b) rg, rs, rd, rp vs t

Solución 1:

Datos: cp* 93
n 0.52
um 0.33
ks 1.7
m 0.03
YC/S 0.08
YP/S 0.45
YP/C 5.6
kd 0.01
t 0.1
tiempo Celulas sustrato producto rg rd rs rp dx/dt ds/dt dp/dt
0 1.000 250.000 0.000 0.318 0.010 -4.002 1.801 0.308 -4.002 1.801
0.1 1.031 249.600 0.180 0.327 0.010 -4.121 1.855 0.317 -4.121 1.855
0.2 1.062 249.188 0.366 0.337 0.011 -4.243 1.910 0.326 -4.243 1.910
0.3 1.095 248.763 0.557 0.347 0.011 -4.369 1.966 0.336 -4.369 1.966
0.4 1.129 248.326 0.753 0.357 0.011 -4.498 2.024 0.346 -4.498 2.024
0.5 1.163 247.877 0.956 0.368 0.012 -4.631 2.084 0.356 -4.631 2.084
0.6 1.199 247.414 1.164 0.378 0.012 -4.767 2.145 0.366 -4.767 2.145
0.7 1.236 246.937 1.378 0.390 0.012 -4.906 2.208 0.377 -4.906 2.208
0.8 1.273 246.446 1.599 0.401 0.013 -5.050 2.272 0.388 -5.050 2.272
0.9 1.312 245.941 1.826 0.413 0.013 -5.197 2.339 0.399 -5.197 2.339
1 1.352 245.422 2.060 0.425 0.014 -5.348 2.407 0.411 -5.348 2.407
1.1 1.393 244.887 2.301 0.437 0.014 -5.503 2.476 0.423 -5.503 2.476
1.2 1.435 244.336 2.549 0.450 0.014 -5.662 2.548 0.435 -5.662 2.548
1.3 1.479 243.770 2.803 0.462 0.015 -5.825 2.621 0.448 -5.825 2.621
1.4 1.524 243.188 3.066 0.476 0.015 -5.992 2.697 0.460 -5.992 2.697
1.5 1.570 242.589 3.335 0.489 0.016 -6.164 2.774 0.474 -6.164 2.774
1.6 1.617 241.972 3.613 0.503 0.016 -6.339 2.853 0.487 -6.339 2.853
1.7 1.666 241.338 3.898 0.518 0.017 -6.520 2.934 0.501 -6.520 2.934
1.8 1.716 240.686 4.191 0.532 0.017 -6.704 3.017 0.515 -6.704 3.017
1.9 1.767 240.016 4.493 0.547 0.018 -6.893 3.102 0.530 -6.893 3.102
2 1.820 239.327 4.803 0.563 0.018 -7.087 3.189 0.544 -7.087 3.189
2.1 1.875 238.618 5.122 0.578 0.019 -7.285 3.278 0.560 -7.285 3.278
2.2 1.931 237.889 5.450 0.594 0.019 -7.487 3.369 0.575 -7.487 3.369
2.3 1.988 237.141 5.787 0.611 0.020 -7.695 3.463 0.591 -7.695 3.463
2.4 2.047 236.371 6.133 0.628 0.020 -7.907 3.558 0.607 -7.907 3.558
2.5 2.108 235.580 6.489 0.645 0.021 -8.124 3.656 0.624 -8.124 3.656
2.6 2.170 234.768 6.854 0.662 0.022 -8.346 3.756 0.641 -8.346 3.756
2.7 2.235 233.933 7.230 0.680 0.022 -8.573 3.858 0.658 -8.573 3.858
2.8 2.300 233.076 7.616 0.699 0.023 -8.805 3.962 0.676 -8.805 3.962
2.9 2.368 232.196 8.012 0.718 0.024 -9.042 4.069 0.694 -9.042 4.069
3 2.437 231.291 8.419 0.737 0.024 -9.283 4.177 0.712 -9.283 4.177
3.1 2.509 230.363 8.837 0.756 0.025 -9.530 4.288 0.731 -9.530 4.288
3.2 2.582 229.410 9.265 0.776 0.026 -9.782 4.402 0.751 -9.782 4.402
3.3 2.657 228.432 9.706 0.797 0.027 -10.038 4.517 0.770 -10.038 4.517
3.4 2.734 227.428 10.157 0.817 0.027 -10.300 4.635 0.790 -10.300 4.635
3.5 2.813 226.398 10.621 0.839 0.028 -10.567 4.755 0.810 -10.567 4.755
3.6 2.894 225.341 11.096 0.860 0.029 -10.838 4.877 0.831 -10.838 4.877
3.7 2.977 224.258 11.584 0.882 0.030 -11.115 5.002 0.852 -11.115 5.002
3.8 3.062 223.146 12.084 0.904 0.031 -11.397 5.128 0.874 -11.397 5.128
3.9 3.150 222.006 12.597 0.927 0.031 -11.683 5.257 0.896 -11.683 5.257
4 3.239 220.838 13.123 0.950 0.032 -11.974 5.388 0.918 -11.974 5.388
4.1 3.331 219.641 13.662 0.974 0.033 -12.270 5.521 0.940 -12.270 5.521
4.2 3.425 218.414 14.214 0.997 0.034 -12.570 5.657 0.963 -12.570 5.657
4.3 3.521 217.157 14.779 1.022 0.035 -12.875 5.794 0.986 -12.875 5.794
4.4 3.620 215.869 15.359 1.046 0.036 -13.185 5.933 1.010 -13.185 5.933
4.5 3.721 214.551 15.952 1.071 0.037 -13.498 6.074 1.034 -13.498 6.074
4.6 3.824 213.201 16.560 1.096 0.038 -13.816 6.217 1.058 -13.816 6.217
4.7 3.930 211.819 17.181 1.122 0.039 -14.138 6.362 1.082 -14.138 6.362
4.8 4.038 210.406 17.817 1.147 0.040 -14.463 6.509 1.107 -14.463 6.509
4.9 4.149 208.959 18.468 1.173 0.041 -14.793 6.657 1.132 -14.793 6.657
5 4.262 207.480 19.134 1.200 0.043 -15.125 6.806 1.157 -15.125 6.806
5.1 4.378 205.967 19.815 1.226 0.044 -15.461 6.957 1.183 -15.461 6.957
5.2 4.496 204.421 20.510 1.253 0.045 -15.799 7.110 1.208 -15.799 7.110
5.3 4.617 202.841 21.221 1.280 0.046 -16.141 7.263 1.234 -16.141 7.263
5.4 4.740 201.227 21.948 1.307 0.047 -16.484 7.418 1.260 -16.484 7.418
5.5 4.866 199.579 22.689 1.335 0.049 -16.830 7.574 1.286 -16.830 7.574
5.6 4.995 197.896 23.447 1.362 0.050 -17.177 7.730 1.312 -17.177 7.730
5.7 5.126 196.178 24.220 1.390 0.051 -17.526 7.887 1.339 -17.526 7.887
5.8 5.260 194.426 25.009 1.417 0.053 -17.876 8.044 1.365 -17.876 8.044
5.9 5.396 192.638 25.813 1.445 0.054 -18.226 8.202 1.391 -18.226 8.202
6 5.536 190.815 26.633 1.473 0.055 -18.576 8.359 1.417 -18.576 8.359
6.1 5.677 188.958 27.469 1.500 0.057 -18.926 8.517 1.444 -18.926 8.517
6.2 5.822 187.065 28.321 1.528 0.058 -19.275 8.674 1.470 -19.275 8.674
6.3 5.969 185.138 29.188 1.556 0.060 -19.623 8.830 1.496 -19.623 8.830
6.4 6.118 183.175 30.071 1.583 0.061 -19.969 8.986 1.522 -19.969 8.986
6.5 6.270 181.178 30.970 1.610 0.063 -20.312 9.141 1.547 -20.312 9.141
6.6 6.425 179.147 31.884 1.637 0.064 -20.653 9.294 1.573 -20.653 9.294
6.7 6.582 177.082 32.813 1.663 0.066 -20.989 9.445 1.598 -20.989 9.445
6.8 6.742 174.983 33.758 1.690 0.067 -21.322 9.595 1.622 -21.322 9.595
6.9 6.904 172.851 34.717 1.715 0.069 -21.649 9.742 1.646 -21.649 9.742
7 7.069 170.686 35.691 1.741 0.071 -21.971 9.887 1.670 -21.971 9.887
7.1 7.236 168.489 36.680 1.766 0.072 -22.287 10.029 1.693 -22.287 10.029
7.2 7.405 166.260 37.683 1.790 0.074 -22.595 10.168 1.716 -22.595 10.168
7.3 7.577 164.001 38.700 1.813 0.076 -22.896 10.303 1.738 -22.896 10.303
7.4 7.751 161.711 39.730 1.836 0.078 -23.187 10.434 1.759 -23.187 10.434
7.5 7.927 159.392 40.774 1.859 0.079 -23.470 10.561 1.779 -23.470 10.561
7.6 8.105 157.045 41.830 1.880 0.081 -23.742 10.684 1.799 -23.742 10.684
7.7 8.284 154.671 42.898 1.900 0.083 -24.003 10.801 1.817 -24.003 10.801
7.8 8.466 152.271 43.978 1.920 0.085 -24.251 10.913 1.835 -24.251 10.913
7.9 8.650 149.846 45.069 1.938 0.086 -24.487 11.019 1.852 -24.487 11.019
8 8.835 147.397 46.171 1.956 0.088 -24.709 11.119 1.867 -24.709 11.119
8.1 9.022 144.926 47.283 1.972 0.090 -24.917 11.212 1.881 -24.917 11.212
8.2 9.210 142.434 48.405 1.987 0.092 -25.108 11.299 1.894 -25.108 11.299
8.3 9.399 139.923 49.534 2.000 0.094 -25.283 11.378 1.906 -25.283 11.378
8.4 9.590 137.395 50.672 2.012 0.096 -25.441 11.448 1.916 -25.441 11.448
8.5 9.781 134.851 51.817 2.023 0.098 -25.580 11.511 1.925 -25.580 11.511
8.6 9.974 132.293 52.968 2.032 0.100 -25.700 11.565 1.932 -25.700 11.565
8.7 10.167 129.723 54.125 2.040 0.102 -25.800 11.610 1.938 -25.800 11.610
8.8 10.361 127.143 55.286 2.045 0.104 -25.878 11.645 1.942 -25.878 11.645
8.9 10.555 124.555 56.450 2.049 0.106 -25.934 11.670 1.944 -25.934 11.670
9 10.750 121.962 57.617 2.052 0.107 -25.968 11.686 1.944 -25.968 11.686
9.1 10.944 119.365 58.786 2.052 0.109 -25.978 11.690 1.943 -25.978 11.690
9.2 11.138 116.767 59.955 2.050 0.111 -25.963 11.683 1.939 -25.963 11.683
9.3 11.332 114.171 61.123 2.047 0.113 -25.923 11.665 1.933 -25.923 11.665
9.4 11.525 111.579 62.290 2.041 0.115 -25.856 11.635 1.926 -25.856 11.635
9.5 11.718 108.993 63.453 2.033 0.117 -25.763 11.593 1.916 -25.763 11.593
9.6 11.910 106.417 64.612 2.023 0.119 -25.643 11.539 1.904 -25.643 11.539
9.7 12.100 103.853 65.766 2.010 0.121 -25.494 11.472 1.889 -25.494 11.472
9.8 12.289 101.303 66.914 1.996 0.123 -25.317 11.393 1.873 -25.317 11.393
9.9 12.476 98.771 68.053 1.979 0.125 -25.111 11.300 1.854 -25.111 11.300
10 12.662 96.260 69.183 1.960 0.127 -24.875 11.194 1.833 -24.875 11.194
10.1 12.845 93.773 70.302 1.938 0.128 -24.609 11.074 1.809 -24.609 11.074
10.2 13.026 91.312 71.410 1.914 0.130 -24.313 10.941 1.784 -24.313 10.941
10.3 13.204 88.881 72.504 1.887 0.132 -23.987 10.794 1.755 -23.987 10.794
10.4 13.380 86.482 73.583 1.858 0.134 -23.630 10.634 1.725 -23.630 10.634
10.5 13.552 84.119 74.646 1.827 0.136 -23.243 10.459 1.691 -23.243 10.459
10.6 13.721 81.795 75.692 1.793 0.137 -22.825 10.271 1.656 -22.825 10.271
10.7 13.887 79.512 76.720 1.757 0.139 -22.377 10.070 1.618 -22.377 10.070
10.8 14.049 77.274 77.726 1.718 0.140 -21.899 9.855 1.578 -21.899 9.855
10.9 14.206 75.085 78.712 1.677 0.142 -21.391 9.626 1.535 -21.391 9.626
11 14.360 72.945 79.675 1.634 0.144 -20.854 9.384 1.490 -20.854 9.384
11.1 14.509 70.860 80.613 1.588 0.145 -20.287 9.129 1.443 -20.287 9.129
11.2 14.653 68.831 81.526 1.540 0.147 -19.693 8.862 1.394 -19.693 8.862
11.3 14.793 66.862 82.412 1.490 0.148 -19.071 8.582 1.342 -19.071 8.582
11.4 14.927 64.955 83.270 1.438 0.149 -18.422 8.290 1.289 -18.422 8.290
11.5 15.056 63.113 84.099 1.384 0.151 -17.748 7.986 1.233 -17.748 7.986
11.6 15.179 61.338 84.898 1.327 0.152 -17.048 7.672 1.176 -17.048 7.672
11.7 15.297 59.633 85.665 1.269 0.153 -16.324 7.346 1.116 -16.324 7.346
11.8 15.408 58.001 86.400 1.209 0.154 -15.578 7.010 1.055 -15.578 7.010
11.9 15.514 56.443 87.101 1.148 0.155 -14.810 6.664 0.992 -14.810 6.664
12 15.613 54.962 87.767 1.084 0.156 -14.021 6.310 0.928 -14.021 6.310
12.1 15.706 53.560 88.398 1.019 0.157 -13.213 5.946 0.862 -13.213 5.946
12.2 15.792 52.239 88.993 0.953 0.158 -12.387 5.574 0.795 -12.387 5.574
12.3 15.872 51.000 89.550 0.885 0.159 -11.543 5.194 0.727 -11.543 5.194
12.4 15.944 49.846 90.070 0.816 0.159 -10.684 4.808 0.657 -10.684 4.808
12.5 16.010 48.777 90.550 0.746 0.160 -9.809 4.414 0.586 -9.809 4.414
12.6 16.069 47.796 90.992 0.675 0.161 -8.920 4.014 0.514 -8.920 4.014
12.7 16.120 46.904 91.393 0.603 0.161 -8.017 3.608 0.441 -8.017 3.608
12.8 16.164 46.103 91.754 0.529 0.162 -7.099 3.195 0.367 -7.099 3.195
12.9 16.201 45.393 92.073 0.454 0.162 -6.166 2.775 0.292 -6.166 2.775
13 16.230 44.776 92.351 0.378 0.162 -5.213 2.346 0.216 -5.213 2.346
13.1 16.252 44.255 92.585 0.300 0.163 -4.234 1.905 0.137 -4.234 1.905
13.2 16.265 43.831 92.776 0.218 0.163 -3.210 1.445 0.055 -3.210 1.445
13.3 16.271 43.510 92.920 0.127 0.163 -2.078 0.935 -0.036 -2.078 0.935
 rg, rd, rs y rp vs t
15.000

10.000

5.000
rg, rd, rs y rp (g/dm3(h))

0.000
0 2 4 6 8 10 12 14
-5.000

-10.000

-15.000

-20.000

-25.000

-30.000
tiempo(h)

rg rd rs rp

Concentracion vs tiempo
300.000

250.000
Concentracion(g/dm3)

200.000

150.000 Celulas
Sustrato
100.000
Producto
50.000

0.000
0 2 4 6 8 10 12 14
tiempo(h)
 2. Bioreactor: Grafique el
modelo de la figura 7.6
y encuentre
a) Dmax
b) Dmax out

Solución 2:
D [S] x Dx
1 0 0.000 5.000 0
ks 0.2 0.05 0.011 4.995 0.24973684
Y x/S 0.5 0.1 0.022 4.989 0.49888889
sf 10 0.15 0.035 4.982 0.74735294
0.2 0.050 4.975 0.995
0.25 0.067 4.967 1.24166667
0.3 0.086 4.957 1.48714286
0.35 0.108 4.946 1.73115385
0.4 0.133 4.933 1.97333333
0.45 0.164 4.918 2.21318182
0.5 0.200 4.900 2.45
0.55 0.244 4.878 2.68277778
0.6 0.300 4.850 2.91
0.65 0.371 4.814 3.12928571
0.7 0.467 4.767 3.33666667
0.75 0.600 4.700 3.525
0.8 0.800 4.600 3.68
0.85 1.133 4.433 3.76833333
0.9 1.800 4.100 3.69
0.95 3.800 3.100 2.945
1 10.000 0.000 0
 10 5

8 4
substrate concentration, s, g/L

Cell production rate xD, g/L-h

Cell concentration, x, g/L

6 3
Concentracion del
sustrato
Concentracion celular
4 2

Output of cells

2 1

0 0
0 0.2 0.4 0.6 0.8 1
Dilution rate, D, 1/h

Dmáx=
Dmáx out =