Soal-soal Mata Kuliah Teknologi Bioproses

2.3. You are filtering a beer which contains two species : bacterial cells 7 x 10-4
cm in diameter an spores 0,3 x 10-4 cm in diameter. You have measured the
spesicific cake resistance of each species :
μαρ0 (bacteria) = 1.3 x 109 kg/sec m3
μαρ0 (spores) = 2.6 x 109 kg/sec m3
other experiments show that for a mixed culture,
μαρ0 = (∑1 ∅𝑖 √𝛼𝑖ρi0𝜇)2
where Фi is the fraction of solute i in all the solutes. Your present beer contains
half the concentration of bacteria as spores.
(a) How long will it take to filter 850 liters of combined beer in a filter of
negligible medium resistance, a pressure drop of 105 N/m2 and 4.0 m2
area?
(b) Would it be faster to first filter the bacteria (with a filter medium which
passes the spores) and then filter the spores?
Solutions :
(a) Asumsi bahwa :
 R medium diabaikan
 Anggap bahwa konsentrasi masing-masing spesies adalah sama (bacteria :
spores = 50 : 50)
 Beer yang akan diperoleh mengandung setengah konsentrasi bakteri
sebagai spora, artinya setengah bakteri akan berubah menjadi spora
(sehingga bacteria : spores = 25 : 75)
 ∆P = 105 N/m2 = 105 kg m/m2 s2
 V = 850 L = 8.5 x 10-4m3 dan A = 4 m2
Sehingga R untuk campuran :
μαρ0 = (∑1 ∅𝑖 √𝛼𝑖ρi0𝜇)2

μαρ0 = (0.25√1.3 × 109 + 0.75√2.6 × 109 )2

= 2233179.112 kg/s m3
Oleh karena itu,
 kg 2
2233179.112 3 8.5 × 10−4 m3
𝑡 = s m
𝑚 +( 4 m2
)
2 × (105 )2 𝑘𝑔
𝑠 𝑚2
t = 7.242 minute

(b) Tidak.

8.2 We have already found that the solubility of bovine serum albumin is 1.2 and
0.26 g/liter in 2.8 and 3.0 M (NH4)2SO4. What is the solubility of this protein
in 3.5 M (NH4)2SO4?
Solution :
Persamaan (8.2-5) menyatakan :
protein
ln [ ] = 21.6 − 7.65[3.5]
solubility
protein
ln [ ] = −5.175
solubility
protein
[ ] = 5.66 × 10−3 𝑔𝑟/𝑙𝑖𝑡𝑒𝑟
solubility

9.3 Imagine a protein molecule of charge (+6) and diffusion coefficient 0.71 x 10 -
6
cm2/sec in excess small electrolyte at 25oC. If this protein is placed under an
electric field of 0.13 V/cm, how fast will it move?
Solution :
𝐷𝑧1 ℱ 𝑑Ф 𝐷𝑧1 ℱ 𝑑Ф
v =( ) = ( )
𝑅𝑇 𝑑𝑧 𝑅𝑇 𝑙
10−6 𝑐𝑚2 𝐶
(0.71 × (+6) (96500
sec ) ) (0.13𝑉)
𝑚𝑜𝑙
v =
𝐽
(8.31 ) (298°𝐾)𝐶 𝑉/𝐽(1𝑐𝑚)
𝑚𝑜𝑙°𝐾
v = 2.158 x 10-5 cm/sec
9.4 In one experiment, it took 13.2 h to desalt by electrodialysis a 1% solution of
bovine serum albumin (mol. Weight 67.000). the initial volume of the
solution was 250 liters and the NaCl concentration was 0.2 M. The power
source was limited to a current of 100 A. What is the final salt concentration?
Solution :
Dik : t = 13.2 h
BM = 67
Vlarutan = 250 L
M NaCl = 0.2 M
I = 100 A
Dit : M NaCl akhir....???
Jwb :
Mol NaCl = 50 mol
NaCl akan dihilangkan sebanyak 99 % = 0.99
Apabila
250 L
𝑄 (𝑙𝑎𝑗𝑢 𝑎𝑙𝑖𝑟) =
13.2 h
Q = 18.94 L/jam
Maka :

100 C 𝑚𝑜𝑙 𝑒𝑙𝑒𝑐𝑡𝑟𝑜𝑛𝑠 1 mol NaCl

( )( )( )
sec 96500 𝐶 2 mol elektron
= 5.18 x 10-4 mol/sec
Jadi
𝐿 1 𝑗𝑎𝑚
5.18 × 10−4 𝑚𝑜𝑙/𝑠𝑒𝑐 = 0.99[𝑀] (18.94 )( )
𝑗𝑎𝑚 3600 𝑠𝑒𝑐
5.208 x 10-3 [M] L/sec = 5.18 x 10-4 mol/sec
5.18 × 10−4 𝑚𝑜𝑙/𝑠𝑒𝑐
[M] =
5.208 × 10−3 𝐿/𝑠𝑒𝑐
[M] = 0.09 M