∆9-TETRAHYDROCANNABINOL

Methanol Solution

Product Number T 4764 Synonyms Tetrahydro-6,6,9-trimethyl-3-pentyl-

6H-dibenzo[b,d]pyran-1-ol;
Lot Number 91K8801
∆1-Tetrahydrocannabinol; ∆9-THC; ∆1-THC
CAS Number [1972-08-3]
Stability If stored at 2-8°C in an air tight container,
Concentration 1.05 mg ∆9-Tetrahydrocannabinol/mL protected from light, decomposition of the
GC grade methanol (conc. verified product is less than 1% in 24 months. After
by HPLC) opening, concentration may change due to
loss of solvent.
Molecular Formula C21H30O2
Expiration Date May 2004
Molecular Weight 314.5
Storage 2-8°C; After opening, transfer contents to
DEA Class Exempt preparation of Class I small vial provided for your convenience.

High Pressure Liquid Chromatography

Column C-18: 5 micron, 4.6 x 25 cm (Supelco 58298-C46)

Mobile Phase
80 % Methanol / 20 % 18 MΩ water
Gradient isocratic
Flow Rate 1.0 mL/min.
Temp ambient
Detector UV @ 210 nm

5 10 15 20 25 30
Time (min.) Perkin-Elmer LC-4 with Hewlett-Packard 3365 Series II Chemstation

FT-IR

100

%
80 T
r
a
n
60 s
m
i
t
40 t
a
n
c
e
20
KBr
4000 3600 3200 2800 2400 2000 1600 1200 800 400

Wavenumbers (cm-1) Mattson Galaxy 3020 FT-IR

∆9-TETRAHYDROCANNABINOL CH3

Methanol Solution OH
Product Number T 4764
Lot Number 91K8801
H C
3
H C O C H (C H ) C H
3 2 2 3 3

Ultraviolet Spectrum Capillary Gas Chromatography

Peak 276.5 nm Column Supelco SPB-1 (Cat. No. 2-4162)

I.D. 0.20 mm Length 15 m Film Thickness 0.20 µm
Absorbance 0.399 Oven Temperature 50°C (2 min.), then 15°/min. to 300°C,
Solvent Methanol hold 11 min.
Injector Temperature 250°C, Split
Dilution 1 : 9 (v/v) Detector Hewlett-Packard 5971
Mass Selective Detector (70 eV, 40-500 m/z)
1.0

0.9

0.8

A 0.7
b
s 0.6
o
r
b 0.5
a
n 0.4
c
e 0.3

0.2

0.1

0
200 240 280 320
220 260 300 5.00 10.00 15.00 20.00 25.00
Wavelength (nm) Time (min.)

Bio-Tek Uvikon XL Hewlett-Packard 5890

70 eV Electron Ionization Mass Spectrum

Abundance
299
35000
231 314
30000

25000

20000 271

15000 243
258
10000
41 91
5000 81 174 193
67 115 128 187 217
55 147 161 285
0
40 60 80 100 120 140 160 180 200 220 240 260 280 300
m/z

Hewlett-Packard 5890 Series II Gas Chromatograph

Hewlett-Packard 5971 Mass Selective Detector
Hewlett-Packard G1034C MS ChemStation (HP-DOS series)

T 4764/91K8801/09/21/01

This product is not for in vitro diagnostic procedures. For R&D use only. Not for drug, household or other uses.
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Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications.
Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply.
Please see reverse side of the invoice or packing slip.