temperature 30 C 40 C 50 C

time, months % Assay 30 C

0 100 100 100
4.61
1 99.2 97.7 96.3
4.6 f(x) = - 0.0081258473x + 4.6043
3 97.2 95.7 92.8
4.59 R² = 0.9901332922
6 95.3 92.3 87.4
4.58
4.57

ln Ca
4.56
30 C
4.55
time, months Ca ln Ca
4.54
0 100 4.605170186
4.53
1 99.2 4.5971380143 0 1 2 3 4 5
3 97.2 4.5767707115
time
6 95.3 4.5570298107

40 C
time, months Ca ln Ca
0 100 4.605170186
1 97.7 4.581901559
3 95.7 4.5612182985
6 92.3 4.5250441415

50 C
time, months Ca ln Ca
0 100 4.605170186
1 96.3 4.5674683188
3 92.8 4.5304466398
6 87.4 4.4704952827

ln k
t 1/T k ln k 0
303.15 0.003298697 0.0081 -4.8158912173 0.003 0.0031 0.0032
313.15 0.0031933578 0.0127 -4.3661532855 -1
323.15 0.0030945381 0.0215 -3.8397023438
-2

Axis Title -3

-4
f(x) = - 4775.
Ea/R=4775.9 R² = 0.995938
-5

-6
Axis Title
 Axis Title -3

-4
f(x) = - 4775.
R² = 0.995938
Ea=4775.9(8.314) -5
39706.8326
-6
Ea=39706.83 J/mol K
Axis Title

Solve for the Activation Energy, EA, and the frequency factor at 28C temperature, calculate the shelf –life in months

k=k0e-Ea/RT
k=0.9e-(39706.83/8.314(301.15))
k=1.167x10-7
 30 C 40 C
4.62
(x) = - 0.0081258473x + 4.6043417989 m= -k
R² = 0.9901332922 -
m= -k=
4.6
4.58
f(x) = - 0.0127387695x + 4.6001804699
R² = 0.9833141055
-0.01

0.0081 4.56

ln Ca
4.54
Column D
Linear 4.52
(Column D) 4.5
4.48
1 2 3 4 5 6 7 0 1 2 3 4 5 6 7
time time

50 C
4.65
m= -k= -0.0215
4.6
f(x) = - 0.0215319588x + 4.5972250037
4.55 R² = 0.9856650329
Column D
ln Ca

4.5 Linear (Column D)

4.45

4.4
0 1 2 3 4 5 6 7
time

ln k vs. 1/T
0
0.003 0.0031 0.0032 0.0033 0.0034
-1
m= -4775.9
-2
Column D
e -3 Linear (Column D)

-4
f(x) = - 4775.8926398688x + 10.9209306544
R² = 0.9959383521
-5

-6
Axis Title
 Column D
e -3 Linear (Column D)

-4
f(x) = - 4775.8926398688x + 10.9209306544
R² = 0.9959383521
-5

-6
Axis Title

ulate the shelf –life in months for an acceptable assay of 90%.

 40 C
m= -k=
5x + 4.6001804699 -0.0127

Column D
Linear (Column D)

3 4 5 6 7
time