Documente Academic
Documente Profesional
Documente Cultură
Tubes (5)
Potato dextrose
Fungi
(slant tube)
Middlebrook 7H11
(slant tube)
OR Mycobacteria
Lowenstein-Jensen
(slant tube)
Thioglycolate broth Anaerobic bacteria
Slides (2)
Swabs (8)
Additional Supplies
Proparacaine drops
Patient labels for each culture and stain
Biohazard specimen bags
Swabbing Technique
Swab the base of the corneal ulcer using gentle pressure with the appropriate swab. There should be
enough pressure to indent the cornea slightly. If the cornea is significantly thinned, apply less pressure
to avoid perforation. Multiple passes can be made to ensure adequate sampling.
Remember to avoid the eyelids and eyelashes to prevent contamination of the culture by skin flora.
Streaking Technique
Container lids should remain on the culture media at all times, both before and after inoculation.
Streak multiple "C" shapes onto the glass slides and agar plates. Gentle pressure should be applied
while streaking, but take care not to puncture the agar media.
Inoculate the agar slants, beginning at the base of the slant, streaking horizontally as you move toward
the top of the tube.
Preparation
1. Gather the supplies
2. Pre-label all media and slides
3. Instill topical proparacaine
4. Remember to avoid the eyelids and eyelashes while swabbing to avoid contamination. Once
unsheathed, the swabs should only contact the corneal ulcer and the medium or glass slide they are
inoculating.
Procedure
HSV/viral media inoculation
1. Use the dry, sterile, polyester tipped swab to sample the ulcer
2. Place the swab deep in the pink viral media
3. Snap off the swab tip, discarding the remaining handle
4. Close the tube, leaving the swab tip in the tube
References
1. Weisenthal R. Basic and Clinic Science Couse: External Disease and Cornea (Section 8). San Francisco:
American Academy of Ophthalmology, 2015.