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A Pilot Study

To Evaluate
Ocean Sequestration of CO2,
Fisheries Restoration, and
Hurricane Intensity Reduction:
One Solution To Three Problems
Abstract. Atmocean, Inc. has developed wave-activated deep ocean pumps, to reduce sea
surface temperature and thus hurricane strength, with possible side benefits of enhancing
biological activity to restore fisheries, and simultaneously absorb atmospheric CO2 while
producing oxygen. Although the undertaking is daring and costly, if successful, this technology
offers potential for enormous benefits for already shrinking shore habitats and marshlands as
well as for humankind. However, benefits often have associated costs. Before any large-scale
deployment, we need to undertake a pilot study to understand all the possible consequences that
our pumps may have on both regional and global climate and on marine communities.

Ocean Science. In general, ocean temperatures Adjacent pump cylinders are connected in a
decrease and nutrients increase with greater matrix, maintaining relative position one to the
depth. This is evident from oceanic upwelling, next from the sea-anchor effect. Periodic
a natural process that enhances biological seafloor anchoring maintains absolute position
activity – for example, the Newfoundland of the matrix within an ocean basin.
Banks are a long-standing productive fishery
just now getting depleted by overuse. Likewise, Figure 1. The Atmocean Pump.
the ocean is by far the largest CO2 sink, with
phytoplankton – which thrive on surface
nutrients - providing the critical link. And, it is
well-established that hurricane intensity
correlates to higher sea surface temperatures.

The Atmocean Pump. We have developed a


wave-activated deep ocean pump to bring
nutrient-rich cold water to the surface. Each
pump comprises a buoy, flexible tube, cylinder
with valve, cable to connect the buoy and
cylinder, and a communications & control
module. Through wave action, deep water is
brought to the surface where it mixes with
adjacent surface water, generally in the top 20
to 50 meters surrounding each pump.

1
Experimental Results. We conducted a primary production will probably lead to
preliminary experiment in Bermuda during increased zooplankton production, which
December 2005 and showed that a smaller subsequently may enhance fish production
prototype of the pumps (Ø 25 cm, 150 m long) (e.g., Ware and Thomson. 1991). Greater fish
worked and actually brought cold water up production could benefit the fishing industry.
(Fig. 4). From that experiment, we estimate
our pumping efficiency is approximately 30%. On the downside, a high nutrient load could
lead to toxic or non-toxic phytoplankton
Figure 4. Results of Test of 500’ Prototype blooms (Paerl 1995). Enhanced primary
Demonstrating Pumping Capability production could result in high day-night
amplitudes of oxygen and pH, which can be
24 harmful to fish and other organisms (Wetzel
Temp Probe attached
23 outside the tube just 2001). Also, when a phytoplankton bloom
Temperature, degrees C.

below the surface


crashes, it often causes anoxia due to
22
Temp Probe attached
degradation of organic material by bacteria
21 inside the tube just (Anderson et al. 2002, Kuypers et al. 2004,
below the surface Temp Probe
20 attached to Justic et al. 2005).
base (500'
19 deep)
Another effect from the increased
18
phytoplankton production could be a net
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transport of carbon from the atmosphere into


the ocean – a process believed to act against
global warming (Martin et al. 1990, Joos et al.
Ocean Surface Nutrient Enhancement. 1991, Watson et al. 2000). However,
During normal conditions, the upper water effectiveness and long-term consequences of
layer of the ocean is low in nutrients, which in enhanced carbon transport into the ocean due to
turn, limits algae production. The deep cold ocean fertilization are a topic of active debate
ocean water that we will bring to the surface to (Peng and Broecker 1991, Sarmiento and Orr
reduce the temperature of the upper water layer 1991, Kerr 1994, Gnanadesikan et al. 2003)
will contain a higher level of nutrients. The and the scope of this issue is complex (Arrigo
effects on the marine ecosystem are expected to and Tagliabue 2005).
be similar to those caused by ocean fertilization
(e.g., iron fertilization experiments (Martin et Hurricane Intensity. Hurricane frequency
al. 1994, Boyd et al. 2004), naturally occurring (Smith 1999) and intensity (Emanuel 2005)
upwelling (Fitzwater et al. 2003), or extreme have increased over the last century which has
wind mixing events caused by hurricanes been attributed to global warming (Michener et
(Walker et al. 2005)). al. 1997) and hurricane intensity is positively
correlated with sea surface temperature
After measuring the degree of change in the (Emanuel 2005).
nutrient load, we will be able to assess whether
the wave-activated pumps might have a Last year’s hurricane Katrina brought that issue
positive impact or negative repercussions on in the center of public attention as she caused
the environment. We expect to see a over 1,400 deaths and was with an estimated
phytoplankton bloom and this enhanced damage of $100 billion the most expensive
primary production may permeate through the natural disaster in US history (National
ecosystem to higher trophic levels. Enhanced Hurricane Center). A cooling of the upper

2
warmer ocean layer during hurricane season
and in the path of the hurricane would deprive Figure 3. Comparison of Precipitation, Wind
the hurricane of its energy source and slow Speeds, and Eyewall Integrity, Hurricane Ivan.
wind speed (DeMaria and Kaplan 1994).
Precipitation (Actual) Precipitation (-2.5º C)
Proposal For Arrays Of Pumps. Based on an
analysis of the paths followed by hurricanes
which hit landfall in the US from 1996-2005,
we believe 84% could have been intercepted
(and intensity likely diminished) by at least one
of the four strategically-positioned arrays of
Atmocean pumps (Figure 2 – arrays are shown
in white on map of the 2005 hurricane paths in
the Gulf of Mexico and Atlantic Ocean).

Over this ten-year period, 32 of 36 (89%) of


hurricanes landfalling in the Gulf of Mexico,
would have been intercepted; 13 of 18 (72%) Windspeed & Eyewall Windspeed & Eyewall
along the Atlantic seaboard; and 9 of 10 (90%) Integrity (actual) Integrity (-2.5º C.)
in the critical middle Atlantic hurricane-
generation region.

Figure 2. Map of 2005 Hurricane Paths and


Proposed Atmocean Pump Arrays.

Eastern Seaboard
Economics. The simplicity of the Atmocean
Pump design, as well as the low-cost ocean
Gulf (2 barge deployment method (Figure 5), are key
Middle features driving system feasibility for CO2
arrays) Atlantic mitigation, fisheries replenishment, and
hurricane intensity reduction. Given
This analysis is based on sensitivity simulations Congress’s preoccupation with Katrina
for Hurricane Ivan (2004) which exhibited damages, we will focus on this application
higher barometric pressure, lower winds, however the reader should be aware of
reduced precipitation, and eye-wall concurrent benefits (at very little added cost) to
disintegration when subjected to two arrays of CO2 mitigation and fisheries enhancement.
2.5º C. cooler sea surface temperatures
prospectively generated by our pumps in the We estimate the deployed cost will be about
Gulf of Mexico (Ritchie 2006). $2,000 per pump. Assuming 100 meter pump
spacing:

3
• Gulf of Mexico dual array 1200km+800km To proceed with development, we need to
@ 60km wide = $2.4 billion. undertake this proposed pilot study in the Gulf
• US East Coast array 1000km @ 100km of Mexico. This study should be funded by a
wide= $2 billion. consortium of federal agencies, the energy
• Atlantic array 2,000km @ 100km wide = industry, ocean fishery producers/distributors,
$4 billion. and insurance. All will benefit tremendously
• Annual maintenance & management from success of this technology.
expense ~ 25% ($2.1 billion).
Pilot Study. In order to prognosticate the
Over 10 years the total investment is thus $29.4
effectiveness of the pumps and to forecast
billion. But this will be offset by $100 billion
unintended consequences of pumping deep
or more savings from reduced hurricane ocean water to the surface, we propose a pilot
damages, and by unknown (probably equal or study. We believe that a 10-week study
greater) value from the carbon credits, as well
deploying 100 wave-activated pumps in a 1
as greater fish production. km2 grid in the Gulf of Mexico near NDBC
Buoy 42038 (located about 160nm SSE of
Figure 5. Pump Array Deployment From Ocean Galveston, TX), will provide the information to
Barges. achieve these objectives.

Our specific goals are:


(1) Calculate efficiency of the pumps in
bringing cold water to the surface;
(2) Investigate the rate at which the cold
water cools the mixed upper layer;
(3) Measure the amount of nutrients that
are transported to the surface;
(4) Study the effects of introducing
nutrient-rich water into the euphotic
zone on lower trophic levels (bacteria,
heterotrophic protozoans,
phytoplankton, zooplankton, and
possibly fish);
(5) Create a model to forecast effects on
Permanent Funding. Once this pilot study regional and global climate as well as
and follow-on rigorous research, modeling, and hurricane intensity using results from
engineering is completed, we suggest Congress numbers 1 and 2. (Initial modeling of
create a “CO2, Fisheries, & Hurricane the effect of cool surface water
Mitigation Trust Fund” akin to our Highway “patches” on three different assumed
Trust Fund that has funded our interstate hurricanes is underway).
highway system since the early 1950’s. (6) Create a model to predict long-term
effects on the biological community
Global warming from CO2, reduced ocean fish utilizing results from numbers 2, 3, and
stocks, and ever-increasing number and 4. Outcomes from the climate model
intensity of hurricanes, are a fact of life. A (number 5) and how they will affect the
dedicated funding source is needed to mitigate biological community will also be
their effects. considered.

4
Study Design week one and nine, and sampling is during
weeks two through seven.
Pumps. Each pump will consist of a 0.77 meter
diameter by 1,000 meter long polyethylene tube Permits & Compliance With Applicable
with wire rope inserted end-to-end; a 0.77 Regulations. We will obtain all necessary
meter diameter by one meter tall metal cylinder permits according to current regulations.
with butterfly valve; and a custom buoy with
approximately 2,000 pounds of buoyancy. Our Data. In order to evaluate the impact on the
engineering calculations suggest this is biological community, we will first take
sufficient to lift cold water entering the cylinder nutrient and temperature profiles at the location
to the surface. The tube is “spooled” onto the where the pumps will be deployed and use this
buoy. For deployment the entire unit is simply information for modeling purposes. The
dropped into the ocean. The heavy base sinks, temperature profile will be taken in 10 m
filling the unwinding tube with ocean water intervals from surface to 1000 m with a CTD
until the design depth is reached. Adjacent (conductivity, temperature, depth) cast. We
units are tethered at the base to maintain will measure total Nitrogen (TN),
uniform spacing. Recovery lines extending Nitrate/Nitrite (NO3/NO2), total Phosphorus
from the pump base to the surface allow us to (TP), Silicon (Si), Iron (Fe) and total organic
pull in the pumps at the conclusion of the carbon (TOC) within the mixed layer
experiment. See www.atmocean.com for more (combined samples at ten equally spaced
details. intervals from surface to the bottom of the
mixed layer), in the middle of the thermocline,
Given each pump’s stored (ready for and at 750 m and 1000 m. Nutrient samples
deployment) dimension of about 42”x56x78” will be taken and stored following the sampling
we propose to utilize two separate vessels for requirement protocols of the Soil, Water, Air
pump deployment/recovery and for acquiring Testing (SWAT) Laboratory and will later be
samples and data. The deployment vessel will analyzed by SWAT following standard
require sufficient open deck to store and procedures (www.swatlab.nmsu.edu).
retrieve 100 pumps (each volume 106 ft_ with
footprint of 16.3 ft_) – about 20’ x 80’ deck Then, during the experiment, we will sample
space. Recovery requires a winch and nine stations in 1-week intervals until
capability to re-spool each pump to its original approximately week 8 (two weeks after the
stored dimension. pumps are discontinued) or until phytoplankton
biomass starts to decline. Three stations will be
Research Vessels. The research vessel should located 5 km into the main wind direction and
provide equipment typically found necessary serve as controls (control stations), three
for ocean biological experiments. For example, stations will be located within the grid of the
the Coast Guard Research Laboratory operates pumps (grid stations) and three stations will be
the R/V Tommy Munro. Information is found marked with drift buoys on days 2, 4, and 6
at: (buoy stations). Meteorological and wave data
http://www.usm.edu/gcrl/research_vessels/tom will be obtained online from the NDBC Buoy
my_munro.php 42038 website. At all stations we will take
profiles of temperature, oxygen, salinity and
These vessels will operate on different pH at 10 m intervals within the mixed water
schedules as deployment/recovery occurs in layer. We will also combine water samples
from the surface to the bottom of the mixed

5
water layer in ten, equally spaced intervals. potentially jellyfish and other large organisms.
Part of those water samples will be used for Finally, at the grid station we will take water
nutrient analyses (see above). Another 100 mL samples from 1000 m for nutrient analyses.
of the water samples will be pre-filtered with a
20 _m mesh screen and preserved with 4% Analysis. The data will be analyzed with
borate-buffered Formalin. The pre-filtered repeated-measures Analysis of Variance
samples will later be stained with acridine (ANOVA) to compare the information from
orange (Suzuki et al. 1993) and 5-50 mL control stations, grid stations and buoy stations.
(depending on amount of suspended matter in Biological response variables will be related to
the samples) will be filtered onto 0.2-_m-pore environmental predictor variables with
size Nuclepore-black membrane filters for canonical correspondence analysis and
enumeration of bacteria under an stepwise regressions (ter Braak 1996). We will
epifluorescence microscope (Hobbie et al. also analyze satellite data for any potential
1977). An additional 250 mL sub-sample will large-scale phytoplankton blooms. Satellite
be preserved in Lugol’s solution for data can pick up differences in temperature and
identification and enumeration of chlorophyll a concentrations due to different
phytoplankton and heterotrophic protozoans ocean colors (e.g., Walker et al. 2005).
(Utermöhl 1958, Karayanni et al. 2004). Five- Satellite data are available from different
hundred milliliters of the water samples will be sensors like the Advanced Very High
filtered over a glass fiber filter (Whatman Resolution Radiometer (AVHRR) aboard
GF/F, 1 _m) on the research vessel and stored National Oceanic and Atmospheric
in opaque vials in a freezer for chlorophyll a Administration’s NOAA-11 and NOAA 14, the
analyses. Chlorophyll a will be measured on Moderate Resolution Imaging Spectrometer
return to the laboratory following standard (MODIS) of Terra-1, the Ocean Color Monitor
protocol using a fluorometer (Clesceri et al. (OCM) of Oceansat-1 or Seaviewing Wide
1998). Finally, one liter of the water sample Field of view Sensor (SeaWiFS) and vary in
will be filtered over a 30 _m mesh. Organisms resolution between 250 m2 and 1.1 km2. Then
on the filter will be back-washed, preserved in we will use the data to model effects on a larger
4% borate-buffered Formalin and later counted scale at various time intervals, temperatures,
under dissecting microscopes. Next to the and nutrient loads. We will use two different
water samples, we will take vertical plankton models, Ecopath with Ecosim (EwE)
hauls from the top of the thermocline to surface (Christensen et al. 2005) and a Generalized
with a 333 _m (Ø 0.2 m) and a 505 _m (Ø 0.6 Additive Model (GAM) (Hastie and Tibshirani
m) mesh-sized bongo nets (Posgay and Marack 1990), both of which have been successfully
1980). These samples will be taken during used to model ecological processes in marine
night time to account for vertical migration environments (e.g., EwE: Bonfil et al. 1998,
(Checkley et al. 1992) and fixed in 4% sugar Pauly et al. 2000, Harvey et al. 2003; GAM:
formaldehyde solution. For a quick estimate of Maravelias and Reid 1997; Richardson et al.
plankton biomass, we will measure plankton 2003). The models will be developed by
displacement volume (Wiebe 1988). For a different personnel and check each other’s
more accurate analysis of zooplankton and validity.
ichthyoplankton composition we will count the
samples under dissecting microscopes. Qualification of Investigators
Furthermore, we will record a 100-m transect at
each station with underwater video cameras at a Wiebke J. Boeing, Ph.D., who will be P.I. for
depth of 10 m to count and identify fish and the Pilot Study, is a faculty member at New

6
Mexico State University. Her previous Philip Fullam, P.E. is a mechanical engineer
employment was with the National Oceanic specializing in prototype design and fabrication
and Atmospheric Administration (NOAA) in and offers a broad perspective on materials
Seattle as a post-doctoral researcher for 1.5 engineering, process engineering, and CAD.
years. There she studied effects of climate
variables on ichthyoplankton dynamics and James K. Herrera, M.S.E.E. provides
assemblage in the Gulf of Alaska. Dr. Boeing instrumentation engineering, embedded
has extensive experience at every trophic level systems design and programming, and sensor &
of the aquatic food web (from bacteria to fish) data acquisition electronics expertise.
and a solid background in fisheries and
statistics. All of her previous work resulted in Consultants & Advisors
peer-reviewed publications in Oecologia,
Limnology and Oceanography, and Canadian David Castel, Ph.D. Principal Development
Journal of Aquatic and Fisheries Science. She Engineer at University of California-San Diego
has also a book chapter providing an overview Coast Data Information Program (CDIP),
about “Factors influencing Groundfish operated by the Scripps Institution of
Dynamics” in press and several other Oceanography. Dr. Castel’s experience
manuscripts on predator-prey interaction, includes involvement with Dr. John Isaacs,
influence of climate change on the aquatic pioneer in development and testing of wave-
community, and factors stabilizing fish driven ocean pumps in the 1960’s and 1970’s.
production are either accepted or submitted.
Isaac Ginis, Ph.D. University of Rhode Island
Philip W. Kithil is an experienced - Graduate School of Oceanography. Dr. Ginis
entrepreneur, business owner/manager, and holds M.S. in Applied Mathematics from
founder/CEO of Atmocean, Inc., and is Kabardino-Balkarski State University (1977),
inventor of the Atmocean Wave Activated Russia and Ph.D. in Geophysics from Institute
Deep Ocean Pump (patents pending). Kithil’s of Experimental Meteorology (1986), Russia.
educational background includes undergraduate He was associated with Princeton
and graduate degrees in Economics. As founder University/Geophysical Fluid Dynamics
and President of Atmocean’s parent company, Laboratory since 1990 as a Visiting Research
Advanced Safety Concepts, Inc., Kithil Scientist before joining Graduate School of
received seven U.S. patents and four Oceanography at University of Rhode Island in
International patents on automotive occupant 1993. His ground-breaking work in developing
sensing and crash sensing technologies, which a coupled hurricane-ocean interaction model
he ultimately licensed to six different has led to significant improvement in hurricane
automotive tier one suppliers. forecasting. The National Weather Service has
embraced this model which has become an
Thomas Pratt, MBA, CPA, is acting CFO of official operational hurricane model for the
Atmocean. Pratt has public and private finance National Hurricane Center in 2001.
experience including Deloitte & Touche,
Revlon, Harbour Group, and Lever Brothers. Elizabeth Ritchie, Ph.D. University of New
He become CEO of Florida Tile before moving Mexico, is a hurricane climatologist in
to Santa Fe, NM to assume the CEO position Electrical & Computer Engineering, and Earth
with Nambe Manufacturing. & Planetary Sciences Departments. She has
provided the simulations for Hurricanes Dennis
(2005) and Ivan (2004) referenced herein.

7
Timetable (2006/2007)

Month 1 2 3 4 5 6 7 8 9 10 11 12
Deployment of pumps X
Conduct experiments, take samples X X
Nutrient, chlorophyll a and plankton X X
displacement analyses
Develop models X X X
Write up results X X
Counting of bacteria, phyto-, zoo- and x X X X X X X
ichthyoplankton
Evaluate underwater videos for fish species X X X
and abundance
Write up results X X X
Refine models X X

2007/2008
The graduate student will finish his/her thesis and a minimum of two manuscripts will be
submitted to journals with a peer-review process.

Budget for experiment assuming a quantity of 100 pumps deployed across 1 km_.

Budget Item: Total


NMSU Costs:
Researcher Salaries $ 124,053
Employee Overhead Costs 15,839
Travel 14,500
Contract and Microscope Charge $ 16,200
Subtotal – Supplies $ 7,800
Publishing costs and miscellaneous $ 2,000
NMSU Indirect $ 81,453
Total NMSU Costs $ 261,845

ATMOCEAN COSTS
Research Vessels $ 295,080
Pump Cost $ 820,000
Permits, Notifications & Legal $75,000
Management, Staff, Consultants, Office, & Misc. $ 103,500
Total ATMOCEAN Costs $ 1,268,580
Total NMSU + ATMOCEAN Costs $ 1,555,425

Assumes R/V Tommy Munro is available, with refrigerator and freezer for samples; winch; 1100
m cable; water sampler; temperature, oxygen, salinity and pH meter.

8
Literature cited

Anderson, D.M., P.M. Glibert and J.M. Burkholder. 2002. Harmful algal blooms and
eutrophication: Nutrient sources, composition, and consequences. Estuaries 25: 704-726.
Arrigo, K.R. and A. Tagliabue. 2005. Iron in the Ross Sea: 2. Impact of discrete iron addition
strategies. Journal of Geophysical Research – Oceans 110: C03010.
Bonfil, R., G. Munro, U.R. Sumaila, H. Valtysson, M. Wright, T. Pitcher, D. Preikshot, N.
Haggan and D. Pauly. 1998. Distant water fleets: An ecological, economic and social
assessment. In: FCRR. Fisheries Centre, University of British Columbia, Vancouver,
Canada. Vol. 6, 111 pp.
Boyd, P.W., C.S. Law, C.S. Wong, Y. Nojiri, A. Tsuda, M. Levasseur, S. Takeda, R. Rivkin, P.J.
Harrison, R. Strzepek, J. Gower, R.M. McKay, E. Abraham, M. Arychuk, J. Barwell-
Clarke, W. Crawford, D. Crawford, M. Hale, K. Harada, K. Johnson, H. Kiyosawa, I.
Kudo, A. Marchetti, W. Miller, J. Needoba, J. Nishioka, H. Ogawa, J. Page, M. Robert,
H. Saito, A. Sastri, N. Sherry, T. Soutar, N. Sutherland, Y. Taira, F. Whitney, S.-K. E.
Wong and T. Yoshimura. 2004. The decline and fate of an iron-induced subarctic
phytoplankton bloom. Nature 428: 549-553.
Checkley, D.M., S. Uye, M.J. Dagg, M.M. Mullin, M. Omori, T. Onbe and M.Y. Zhu. 1992. Diel
variation of the zooplankton and its environment at neritic stations in the Inland Sea of
Japan and the North-West Gulf of Mexico. Journal of Plankton Research 14: 1-40.
Christensen, V., C.J. Walters and D. Pauly. 2005. Ecopath with Ecosim: A users guide. Fisheries
Centre, University of British Columbia, Vancouver. November 2005 edition. 154 p.
Clesceri, L.S., A.E. Greenberg and A.D. Eaton. 1998. Standard methods for the examination of
water and wastewater. American Public Health Association, 20th edition.
DeMaria, M. and J. Kaplan. 1994. Sea surface temperature and the maximum intensity of
Atlantic tropical cyclones. Journal of climate 7: 1324-1334.
Emanuel, K. 2005. Increasing destructiveness of tropical cyclones over the past 30 years. Nature
435: 686-688.
Fitzwater, S.E., K.S. Johnson, V.A. Elrod, J.P. Ryan, L.J. Coletti, S.J. Tanner, R.M. Gordon and
F.P. Chavez. 2003. Iron, nutrient and phytoplankton biomass relationships in upwelled
waters of the California coastal system. Continental Shelf Research 23: 1523-1544.
Gnanadesikan, A., J.L. Sarmiento and R.D. Slater. 2003. Effects of patchy ocean fertilization on
atmospheric carbon dioxide and biological production. Global Biochemical Cycles 17:
1050.
Harvey, C.J., S.P. Cox, T.E. Essington, S. Hansson and J.F. Kitchell. 2003. An ecosystem model
of food web and fisheries interactions in the Baltic Sea. Journal of Marine Science 60:
939-950.
Hastie, T.J. and R.J. Tibshirani. 1990. Generalized additive models. Chapman & Hall/CRC, New
York.
Hobbie, J.E., R.J. Daley and S. Jasper. 1977. Use of Nuclepore filters for counting bacteria by
fluorescence microscopy. Applied Environmental Microbiology 33: 1225-1228.
Joos, F., J.L. Sarmiento and U. Siegenthaler. 1991. Estimates of the effect of Southern-Ocean
iron fertilization on atmospheric CO2 concentrations. Nature 349: 772-775.
Justic, D., N.N. Rabalais and R.E. Turner. 2005. Coupling between climate variability and
coastal eutrophication: Evidence and outlook for the northern Gulf of Mexico. Journal of
Sea Research 54: 25-35.

9
Karayanni, H., U. Christaki, F. Van Wambeke and A.P. Dalby. 2004. Evaluation of double
formalin-Lugol’s fixation in assessing number and biomass of ciliates: an example of
estimations at mesoscale in NE Atlantic. Journal of Microbiological Methods 56: 349-
358.
Kerr, R.A. 1994. Iron fertilization: a tonic, but no cure for the greenhouse. (Adding trace
amounts of iron to ocean to control greenhouse effect). Science 263: 1089-1090.
Kuypers, M.M.M., L.J. Lourens, W.R.C. Rijpstra, R.D. Pancost, I.A. Nijenhuis and J.S.S.
Damste. 2004. Orbital forcing of organic carbon burial in the proto-North Atlantic during
oceanic anoxic event 2. Earth and Planetary Science Letters 228: 465-482.
Maravelias, C.D. and D.G. Reid. 1997. Identifying the effects of oceanographic features and
zooplankton on prespawning herring abundance using generalized additive models.
Marine Ecology Progress Series 147: 1-9.
Martin, J.H., K.H. Coale, K.S. Johnson, S.E. Fitzwater, R.M. Gordon, S.J. Tanner, C.N. Hunter,
V.A. Elrod, J.L. Nowicki, T.L. Coley, R.T. Barber, S. Lindley, A.J. Watson, K. Vanscoy,
C.S. Law, M.I. Liddicoat, R. Ling, T. Stanton, J. Stockel, C. Collins, A. Anderson, R.
Bidigare, M. Ondrusek, M. Latasa, F.J. Millero, K. Lee, W. Yao, J.Z. Zhang, G.
Friederich, C. Sakamoto, F. Chavez, K. Buck, Z. Kolber, R. Greene, P. Falkowski, S.W.
Chisholm, F. Hodge, R. Swift, J. Yungel, S. Turner, P. Nightingale, A. Hatton, P. Liss
and N.W. Tindale. 1994. Testing the iron hypothesis in ecosystems of the equatorial
Pacific Ocean. Nature 371: 123-129.
Martin, J.H., R.M. Gordon and S.E. Fitzwater. 1990. Iron in Antarctic waters. Nature 345: 156-
158.
Michener, W.K., E.R. Blood, K.L. Bildstein, M.M. Brinson and L.R. Gardner. 1997. Climate
change, hurricanes and tropical storms, and rising sea levels in coastal wetlands.
Ecological Applications 7: 770-801.
Paerl, H.W. 1995. Coastal eutrophication in relation to atmospheric nitrogen deposition – current
perspectives. Ophelia 41: 237-259.
Pauly, D., V. Christensen and C. Walters. 2000. Ecopath, Ecosim, and Ecospace as tools for
evaluating ecosystem impact of fisheries. ICES Journal of Marine Science 57: 697-706.
Peng, T.H. and W.S. Broecker. 1991. Dynamic limitations on the arctic iron fertilization
strategy. Nature 349: 227-229.
Posgay, J.A. and R.R. Marack. 1980. The MARMAP bongo zooplankton samplers. Journal of
Northwest Atlantic Fishery Science 1: 91-99.
Richardson, A.J., N.F. Silulvane, B.A. Mitchell-Innes and F.A. Shillington. 2003. A dynamic
quantitative approach for predicting the shape of phytoplankton profiles in the ocean.
Progress in Oceanography 59: 301-319.
Ritchie, E.A., 2006. A Numerical Modeling Study of the Impact of cooler SST’s on the Intensity
of Hurricane Ivan (2004) in the Gulf of Mexico. 20 April 2006. (Unpublished).
Sarmiento, J.L. and J.C. Orr. 1991. 3-dimensional simulations of the impact of Southern-Ocean
nutrient depletion on atmospheric CO2 and ocean chemistry. Limnology and
Oceanography 36: 1928-1950.
Smith, E. 1999. Atlantic and east coast hurricanes 1900-98: A frequency and intensity study for
the twenty-first century. Bulletin of the American Meteorological Society 80: 2717-2720.
Stone, G.W., J.M. Grymes, J.R. Dingler and D.A. Pepper. 1997. Overview and significance of
hurricanes on the Louisiana coast, USA. Journal of Coastal Research 13: 656-669.

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Suzuki, M.T., E.B. Sherr and B.F. Sherr. 1993. DAPI direct counting underestimates bacterial
abundances and average cell-size compared to AO direct counting. Limnology and
Oceanography 38: 1566-1570.
ter Braak, C.J.F. 1996. Unimodal models to relate species to environment. DLO-Agricultural
Mathematics Group, Wageningen.
Utermöhl, H. 1958. Zur Vervollkommung der quantitativen Phytoplankton-Methodik.
Internationale Vereinigung für Theoretische und Angewandte Limnologie, Mitteilungen
9: 1-38.
Walker, N.D., R.R. Leben and S. Balasubramanian. 2005. Hurricane-forced upwelling and
chlorophyll a enhancement within cold-core cyclones in the Gulf of Mexico. Geophysical
Research Letters 32: L18610.
Ware, D.M. and R.E. Thomson. 1991. Link between long-term variability in upwelling and fish
production in the Northeast Pacific Ocean. Canadian Journal of Fisheries and Aquatic
Sciences 48: 2296-2306.
Watson, A.J., D.C.E. Bakker, A.J. Ridgwell, P.W. Boyd and C.S. Law. 2000. Effect of iron
supply on Southern Ocean CO2 uptake and implications for glacial atmospheric CO2.
Nature 407: 730-733.
Wetzel, R.G. 2001. Limnology: Lake and River Ecosystems. Saunders College Publishing.
Wiebe, P.H. 1988. Functional regression equations for zooplankton displacement volume, wet
weight, dry-weight, and carbon – a correction. Fishery Bulletin 86: 833-835.

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