Lineweaver- Burke Langmuir Eaddie- Hofstee

Substrat (M) r(mol/l·s) 1/r 1/S Substrat (M) S/r r/S

0.1 34.2 0.02923977 10 0.1 0.00292398 342
0.2 61.1 0.01636661 5 0.2 0.00327332 305.5
0.3 82.6 0.01210654 3.33333333 0.3 0.00363196 275.333333
0.4 101.7 0.00983284 2.5 0.4 0.00393314 254.25
0.5 124.7 0.00801925 2 0.5 0.00400962 249.4
0.6 141.4 0.00707214 1.66666667 0.6 0.00424328 235.666667
0.7 158.4 0.00631313 1.42857143 0.7 0.00441919 226.285714
0.8 161.6 0.00618812 1.25 0.8 0.0049505 202
0.9 176.6 0.00566251 1.11111111 0.9 0.00509626 196.222222
1 195.6 0.00511247 1 1 0.00511247 195.6
1.1 192.6 0.00519211 0.90909091 1.1 0.00571132 175.090909
1.2 209.6 0.00477099 0.83333333 1.2 0.00572519 174.666667
1.3 211.8 0.00472144 0.76923077 1.3 0.00613787 162.923077
1.4 216 0.00462963 0.71428571 1.4 0.00648148 154.285714
1.5 228.2 0.00438212 0.66666667 1.5 0.00657318 152.133333
1.6 239.8 0.00417014 0.625 1.6 0.00667223 149.875
1.7 240 0.00416667 0.58823529 1.7 0.00708333 141.176471
1.8 258.6 0.00386698 0.55555556 1.8 0.00696056 143.666667
1.9 256.9 0.00389257 0.52631579 1.9 0.00739587 135.210526
2 259.6 0.00385208 0.5 2 0.00770416 129.8
addie- Hofstee
r(mol/l·s) Lineweaver-Burke
34.2 0.035
61.1
0.03
82.6 f(x) = 0.0026960867x + 0.0026279958
0.025 R² = 0.9986694663
101.7
124.7 0.02

1/r
141.4 0.015
158.4 0.01
161.6
0.005
176.6
195.6 0
0 2 4 6 8 10 12
192.6
1/S
209.6
211.8
216
228.2 Langmuir
239.8 0.009
240 0.008
258.6 0.007 f(x) = 0.0024428918x + 0.0028369094
R² = 0.9917768592
256.9 0.006
259.6 0.005
S/r

0.004
0.003
0.002
0.001
0
0 0.5 1 1.5 2 2.5
S

Eadie - Hofstee
300

250
f(x) = - 1.1102243433x + 399.6551705085
R² = 0.9780381805
200

150
r

100

50

0
100 150 200 250 300 350 400
r/S
0
100 150 200 250 300 350 400
r/S
 Km/rm =0,0027
1/rm = 0,0026

rm 384.615385
Km 1.03846154

10 12

Km/rm=0,0028
1/rm=0,0024

rm 416.666667
Km 1.16666667

2.5

rm 399.66
Km 1.1102

350 400
350 400
 Absorbància 340 nm
NAD+ 0,5 min 1,5 min 2,5 min 3,5 min 4,5 min
1.5 0.009 0.025 0.041 0.055 0.069
2 0.012 0.034 0.055 0.074 0.091
2.5 0.015 0.042 0.068 0.092 0.114
3 0.017 0.051 0.081 0.11 0.137
5 0.029 0.084 0.135 0.182 0.226
10 0.056 0.164 0.264 0.357 0.445

Concentració NADH
NAD+ 0,5 min 1,5 min 2,5 min 3,5 min 4,5 min
1.5 1.446945E-06 4.0193E-06 6.5916E-06 8.8424E-06 1.1093E-05
2 1.92926E-06 5.4662E-06 8.8424E-06 1.1897E-05 1.463E-05
2.5 2.411576E-06 6.7524E-06 1.0932E-05 1.4791E-05 1.8328E-05
3 2.733119E-06 8.1994E-06 1.3023E-05 1.7685E-05 2.2026E-05
5 4.662379E-06 1.3505E-05 2.1704E-05 2.926E-05 3.6334E-05
10 9.003215E-06 2.6367E-05 4.2444E-05 5.7395E-05 7.1543E-05

So Ro 1/So 1/Ro
1.5 2.893891E-06 0.66666667 345555.556
2 3.858521E-06 0.5 259166.667 400000

2.5 4.823151E-06 0.4 207333.333 350000

f(x)
3 5.466238E-06 0.33333333 182941.176 300000 R²
5 9.324759E-06 0.2 107241.379
250000
10 1.800643E-05 0.1 55535.7143
200000

150000

100000

50000

0
0 0.1
 5,5 min
0.081
0.108
0.135
0.161
0.267
0.526

5,5 min
1.3023E-05
1.7363E-05
2.1704E-05
2.5884E-05
4.2926E-05
8.4566E-05

km/rm = 509133
Michaelis-Menten
1/rm = 6280,2
400000

350000 rmax= 0.00015923

f(x) = 509132.906280634x + 6280.2386341346
300000 R² = 0.9988672738 Km= 81.0695519

250000

200000

150000

100000

50000

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
Temps(min) [glucosa], mM 1.2
1 0.05
1
5 0.23 f(x) = 0.033026835x +
R² = 0.9973355794
10 0.38
0.8
15 0.52
30 1.03 0.6

0.4

0.2

0
0 5 10
 Exp data
1.2

1 f(x) = 0.033026835x + 0.0390726125

R² = 0.9973355794
0.8

0.6

0.4

0.2

0
0 5 10 15 20 25 30 35
 s 0.049 0.0963 0.181 0.266 0.457
R 0.0051 0.0081 0.0121 0.0141 0.0135
1/S 20.4081633 10.384216 5.52486188 3.7593985 2.18818381
1/r 196.078431 123.45679 82.6446281 70.9219858 74.0740741

S baixa
250 S alta
120
200
f(x) = 7.5649990016x + 42.4805241528 f(x) = 100.8544503539x + 27.3152044644
R² = 0.9990470876 100 R² = 0.9946149303
150
80

100 60

50 40

20
0
2 4 6 8 10 12 14 16 18 20 22
0
0.4 0.45 0.5 0.55 0.6 0.65 0.7
 exp data
0.69 0.846 0.016
0.0105 0.0088
0.014
1.44927536 1.1820331
95.2380952 113.636364 0.012

0.01

0.008

0.006

0.004

0.002

0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9
S alta

exp data inversas

= 100.8544503539x + 27.3152044644
0.9946149303 250

200

150

100

50
0.5 0.55 0.6 0.65 0.7 0.75 0.8 0.85 0.9

0
0 5 10 15 20 25
.6 0.7 0.8 0.9

20 25