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DNA and R
May have a
envelope
Have their
metabolism
May contai
enzymes fo
replication
Cell wall
s Viruses co
either DNA
RNA but n
Some may
an envelop
Unlike bac
they do not
cell wall or
own metab
They may
enzymes fo
replication
2. The foll
are DNA v
Herpesviru
Orthomyxo
Enteroviru
Hepadnavi
Parvovirus
s Herpesviru
Hepadnavi
are double
stranded D
viruses.
Parvovirus
single stran
DNA virus
Orthomyxo
and enterov
are singles
stranded R
viruses.
3. The foll
are RNA v
Picornaviru
Adenoviru
Papillomav
Rhabdoviru
Rotaviruse
s Adenoviru
papillomav
are double
standed DN
viruses.
Picornaviru
and rhabdo
are single
stranded R
viruses.
Rotaviruse
double stra
RNA virus
4. Live
attenuated
vaccines a
available a
the followi
viruses
Influenza A
Hepatitis B
Rubella Vi
Yellow Fev
Virus
Varicella-Z
Virus
s Live atten
vaccines ar
available a
rubella, yel
fever, and
varicella-zo
viruses. Inf
A viral vac
are either
inactivated
subunit vac
The vaccin
current use
against hep
are recomb
subunit vac
5. The foll
antiviral a
is active ag
the followi
virus
Lamivudin
HIV
Lamivudin
HBV
Amantidin
influenza B
Ribavirin a
RSV
Acyclovir a
HSV
s Lamivudin
reverse
transcriptas
inhibitor ac
against bot
and HBV.
Amantidin
active agai
influenza A
Ribavirin i
against RS
acyclovir a
HSV
6. Herpes Simplex Encephalitis
Comments Herpes Simplex Encephalitis commonly affect the temporal lobe. Culture of the CSF is usually unsuccess
is a much better choice. A diagnosis can also be made by the detection of HSV-specific antibodies in the C
Encephalitis should be treated with IV acyclovir.
Comments VZV causes a vesicular rash. It does not respond to AZT as it does not possess reverse transcriptase. It re
sensory ganglia following primary infection. It is rare for more than one episode of shingles to occur. Pati
infectious.
8. Cytomegalovirus (CMV)
False Is teratogenic
Comments Primary CMV infection is usually asymptomatic. Occasionally, an infectious mononucleosis-like syndrom
primary infection. CMV is well known to cause severe disease in immunocompromised individuals, notab
recipients and patients with AIDS. Although it may cause severe congenital infection, it is not teratogenic
formed fetal organs. It is not associated with Kawaski's disease.
9. Epstein-Barr Virus (EBV) is associated with
True Hepatitis
Comments Parvovirus is associated with Fifth disease (erythema infectiosum). HHV-6 is associated with Sixth diseas
HHV-8 is associated with Kaposi's sarcoma. Rarely, in adults, primary HHV-6 infection may result in an
mononucleosis-like illness. EBV is associated with oral leukoplakia
11. Adenoviruses
Comments Human papillomaviruses are associated with genital cancers. Adenoviruses type 40 and 41 are associated
Adenoviruses may also cause conjunctivitis and pneumonia. Human papillomaviruses are associated with
12. Human Papillomaviruses
Comments HPV-16 and HPV-18 are associated with genital cancers. Warts caused by papillomaviruses may respond
although it is rarely used. Huamn Papillomaviruses cannot be grown in cell culture. Polyomavirus JC is as
True Myocarditis
True Meningitis
Comments Influenza A Virus may undergo antigenic shift and antigenic drift. Antigenic shifts result in pandemics. In
respond to rimantidine, which is a similar compound to amantidine. It will also respond to newly available
inhibitors. Because of constant antigenic changes, vaccination will not confer lifelong immunity; it will la
True Croup
True Bronchiolitis
False Diarrhoea
Comments Paramyxoviruses most commonly cause croup. They may occasionally cause bronchiolitis and pneumoni
Comments RSV will not respond to amantidine. It most commonly cause bronchiolitis and pneumonia. Occasionally
There is no vaccine available.
17. Parvoviruses
False Is teratogenic
Comments Parvovirus causes erythema infectiousum. Although it may cause abortion and stillbirths, it is not teratoge
aplastic crisis in persons with haemolytic anaemias
Comments Measles causes a maculopapular rash. It may cause encephalitis. It does not respond to acyclovir. Suscept
be protected by HNIG. A live attenuated vaccine is now given as part of universal vaccination in many co
True The rash of rubella is similar to that caused by parvo and enteroviruses
True Is teratogenic
True Congenital rubella is diagnosed by the finding of rubella-specific antibody in the cord blood of infants
Comments The rash of rubella is similar to that caused by parvo and enteroviruses. It is teratogenic, the classical triad
rubella consists of eye, ear and heart defects. Congenital rubella can be diagnosed by the finding of rubell
the cord blood of infants.Infants with congenital rubella poses a great infectious risk since they may excre
1 year.
Comments Human T-lymphotropic virus 1 (HTLV-1) is associated with Adult T-cell lymphoma and Tropical Spastic
True Dementia
Comments HIV envelope antibody remains high at a constant level throughout the period of
infection and is thus of no use as a prognostic marker. HIV-p24 antigen had been widely
used as a prognostic marker in the past but has now been supplanted by HIV plasma RNA
(viral load). The CD4 count provides important information on the stage of the disease.
False Hepatitis A
True Hepatitis B
True Hepatitis C
Comments A chronic carrier state may occur with HBV and HCV infection.
24. The following markers are usually present in a hepatitis B carrier with
chronic active hepatitis
True HbeAg
True HBV-DNA
True HbsAg
Comments In patients with chronic active hepatitis, HBV replication is present and hence HBV-DNA
and HbeAg. HbsAg is present. Anti-HbcIgM is normally absent although it may
occasionally be detected during periods of exacerbations. Anti-HBc IgG is present.
25. The following statements are true
Comments Chronic HBV and HCV infection may respond to interferon therapy. Chronic HCV
infection may respond to ribavirin therapy; however ribavirin is usually given with
interferon. Hepatitis Delta infection may be prevented by vaccination against HBV. There
is no vaccine available against HEV.
True Hepatitis A
True HIV
True HTLV-1
True HBV
True HCV
True Enteroviruses are one of the commonest causes of CNS infections in childhood
Comments Meningitis usually occurs with encephalitis. Enteroviruses are one of the commonest causes of
CNS infections in childhood. HSV encephalitis result from invasion of the virus rather than an
allergic postinfectious event as in the case of measles. The detection of antibody in the CSF is a
useful diagnostic marker
28. The following viruses are associated with gastroenteritis
True Astroviruses
False Picornviruses
True Adenoviruses
True Rotaviruses
Comments Although enteroviruses may be found in faeces, they are not associated with gastroenteritis
False Polioviruses
False CMV
True Hantaviruses
Comments Rabies may be transmitted to humans from a variety of mammals. Hantaviruses and Lassa Fever
Virus may be transmitted to humans by rodents.
Comments The majority of human rabies cases world-wide result from dog bites. Infection may be prevented
by active and passive immunisation. Although live attenuated vaccines are available for
vaccinating animals, inactivated vaccines are used in humans for safety reasons. May be diagnosed
by serology.
.
1. Viruses may contain
True
True
True
True
False
Comments
True
False
False
True
True
Comments
False
True
True
True
True
Comments
True
False
False
True
True
Comments
True
False
True
False
True
Comments
True Seroconversion
Comments A diagnosis of a primary viral infection may be made by the detection of IgM and/or
seroconversion.
7. The following are examples of viral genome detection (molecular methods)
Comments Southern blot, branched DNA, and PCR are viral genome detection methods. Western blot and
RIBA are serological assays
False For cytomegalovirus (CMV), the cytopathic (CPE) effect usually appears within 24-48 hours
True For some viruses, the CPE is so characteristic that so further identification is required.
False A given virus always produce identical CPE in different cell cultures
Comments It takes 1-3 weeks for CMV-specific CPE to appear. For some viruses such as herpes simplex, the
CPE is so characteristic that so further identification is required.
Comments Polioviruses can be typed by neutralization tests and hybridization with specific nucleic acid probes.
SRH and HAI are not used. The DEAFF test is used for the isolation of CMV
10. Immunofluorescence techniques can be used to detect the following directly from
the specimen.
True Chlamydia
True CMV
True Respiratory Syncytial Virus (RSV)
11. The following statements are true for the haemagglutination-inhibition (HAI)
test.
Comments HAI is a quantitative test. It is widely used in rubella serology. It is usually more specific than
CFT. Treatment of patient serum is necessary to remove non-specific inhibitors.
False The presence of cytopathic effect is the only way to detect a virus
False The haemagglutination inhibition test is the mainstay of identification of a respiratory syncytial
virus (RSV) isolate
False Whole blood is the specimen of choice for many common viruses
Comments Viruses may also be cultured in eggs and animals. The presence of virus may be detected by
CPE as well as haemadsoption, and presence of viral particles and antigen.
True dNTPs
True Mg++ ions
Comments Denaturation, annealing, and ligation steps occur in LCR. PCR consists of Denaturation,
annealing, and extension steps. It requires dNTP, Mg++, taq polymerase, and target-specific
oligonucleotide primers.
Comments Nested PCR, RT-PCR and quantitative PCR are modifications of the PCR protocol. bDNA and
3SR are alternative amplification techniques.
True Separate areas for master mix, template, and PCR product operation
True Dedicated pipettes for master mix, template, and PCR products
Comments Exponential amplification occurs in PCR, LCR and NASBA/3SR. Therefore these techniques
are extremely liable to contamination and are more sensitive than branched DNA, where linear
amplification occurs.
Comments Diagnosis of infectious mononucleosis is usually made by the Paul-Bunell where heterophil
antibodies are detected and by the detection of EBV-IgM. VCA-IgG avidity test may be used to
confirm equivocal cases. Detection of elevated titres of VCA-IgA correlates with an increased
risk for nasopharyngeal carcinoma and is used as a screening test. Seroconversion against
EBNA does not take place until months after the initial illness.
18. Viruses that routinely establish latent infections in human sensory ganglia
include
Comments All the above herpesviruses can cause latent infections. However, only HSV 1+2, and VZV
routinely establish latent infections in human sensory ganglia.
False Rotaviruses
True Adenoviruses
False HTLV-1
True RSV
Comments Rotaviruses cause diarrhoea. HTLV-1 is associated with adult T-cell leukaemia and tropical
spastic paraparesis.
False Enteroviruses
True Adenoviruses
True Astroviruses
True Rotaviruses
Comments Although enteroviruses may be found in faeces, they are not associated with gastroenteritis. The
other viruses are associated with gastroenteritis.
False HTLV-1
True Hantaviruses
False Poliomyelitis
True Rabies
Comments Influenza A H5N1 is otherwise known as the "bird flu" It afflicted Hong Kong in late 1997 and
was transmitted from poultry to humans. Hantaviruses is transmitted from rodents to humans and
rabies can be transmitted from various mammals to humans.
23. A chronic carrier state may occur in the following:
False Hepatitis A
True Hepatitis B
True Hepatitis C
False Hepatitis E
Comments A chronic carrier state can exist in hepatitis B, C and Delta Virus infections
False Rubella
True HIV
False Measles
True HBV
True HCV
Comments HIV, HBV and HCV are transmitted by blood. Rubella and measles are transmitted via respiratory
droplets
Comments HIV envelope antibody remains high throughout the course of HIV infections and thus does not
carry any prognostic value. HIV-p24 antigen had been widely used in the past as a prognostic
marker but it has been superseded by HIV viral load. CD4 count plays an important role in telling
us what stage the disease is at.
26. The following markers are usually present in a hepatitis B carrier with chronic
active hepatitis
True HbsAg
False HbsAb
True HBV-DNA
True HbeAg
Comments In patients with chronic active hepatitis, HBV replication is present and hence HBV-DNA and
HbeAg. HbsAg is present. Anti-HbcIgM is normally absent although it may occasionally be
detected during periods of exacerbations.
True Enteroviruses are one of the commonest causes of CNS infections in childhood
False Electron microscopy of the cerebrospinal fluid (CSF) is a useful diagnostic test
Comments Meningitis usually occurs together with encephalitis. Enteroviruses are one of the commonest
causes of CNS infections in childhood. Electron microscopy is too insensitive to be useful as a
diagnostic test. PCR assays for HSV are now routinely used for the diagnosis of herpes simplex
encephalitis. The detection of antibody in the CSF is a useful diagnostic marker
False The rash caused by parvovirus B19 is due to the presence of virus in the skin
True Herpes simplex virus (HSV) infection is usually diagnosed by viral culture
False Can have serious side effects when occurring in a woman in the third trimester of pregnancy
True May be acquired by having close contact with an infant with congenital rubella syndrome
Comments Rubella infection can be asymptomatic. It may be indistinguishable clinically from parvovirus
B19 infection. The most serious effects occur when a pregnant woman is infected in the first
trimester of pregnancy. It is usually preventable by vaccination. Infants with congenital rubella
syndrome pose a great infectious risk since they may excrete virus in great quantities up to 1 year
of age.
Comments Rubella immunity may be determined by an enzyme immunoassay. CFT is not commonly used for
the diagnosis of rubella infection; Haemagglutination-inhibition tests are. IgM antibody is usually
detectable after the onset of rash Rubella virus can be cultured although this is rarely used as a
method of diagnosis. Pre-natal diagnosis of rubella in a foetus such as amniocentesis involve some
degree of risk to the fetus.
True Seroconversion
Comments A diagnosis of a primary viral infection may be made by the detection of IgM and/or
seroconversion.
Comments Southern blot, branched DNA, and PCR are viral genome detection methods. Western blot and
RIBA are serological assays
False For cytomegalovirus (CMV), the cytopathic (CPE) effect usually appears within 24-48 hours
True For some viruses, the CPE is so characteristic that so further identification is required.
False A given virus always produce identical CPE in different cell cultures
Comments It takes 1-3 weeks for CMV-specific CPE to appear. For some viruses such as herpes simplex, the
CPE is so characteristic that so further identification is required.
Comments Polioviruses can be typed by neutralization tests and hybridization with specific nucleic acid probes.
SRH and HAI are not used. The DEAFF test is used for the isolation of CMV
10. Immunofluorescence techniques can be used to detect the following directly from
the specimen.
True Chlamydia
True CMV
11. The following statements are true for the haemagglutination-inhibition (HAI)
test.
Comments HAI is a quantitative test. It is widely used in rubella serology. It is usually more specific than
CFT. Treatment of patient serum is necessary to remove non-specific inhibitors.
False The presence of cytopathic effect is the only way to detect a virus
False The haemagglutination inhibition test is the mainstay of identification of a respiratory syncytial
virus (RSV) isolate
False Whole blood is the specimen of choice for many common viruses
Comments Viruses may also be cultured in eggs and animals. The presence of virus may be detected by CPE
as well as haemadsoption, and presence of viral particles and antigen.
True dNTPs
Comments Denaturation, annealing, and ligation steps occur in LCR. PCR consists of Denaturation,
annealing, and extension steps. It requires dNTP, Mg++, taq polymerase, and target-specific
oligonucleotide primers.
Comments Nested PCR, RT-PCR and quantitative PCR are modifications of the PCR protocol. bDNA and
3SR are alternative amplification techniques.
True Separate areas for master mix, template, and PCR product operation
True Dedicated pipettes for master mix, template, and PCR products
Comments Exponential amplification occurs in PCR, LCR and NASBA/3SR. Therefore these techniques are
extremely liable to contamination and are more sensitive than branched DNA, where linear
amplification occurs.
Comments Diagnosis of infectious mononucleosis is usually made by the Paul-Bunell where heterophil
antibodies are detected and by the detection of EBV-IgM. VCA-IgG avidity test may be used to
confirm equivocal cases. Detection of elevated titres of VCA-IgA correlates with an increased risk
for nasopharyngeal carcinoma and is used as a screening test. Seroconversion against EBNA does
not take place until months after the initial illness.
18. Viruses that routinely establish latent infections in human sensory ganglia
include
Comments All the above herpesviruses can cause latent infections. However, only HSV 1+2, and VZV
routinely establish latent infections in human sensory ganglia.
Comments Rapid diagnosis of CMV disease include CMV pp65 antigenaemia, DEAFF test, and Detection of
CMV-DNA in blood by PCR.
False Rotaviruses
True Adenoviruses
False HTLV-1
True RSV
Comments Rotaviruses cause diarrhoea. HTLV-1 is associated with adult T-cell leukaemia and tropical
spastic paraparesis.
False Enteroviruses
True Adenoviruses
True Astroviruses
True Rotaviruses
Comments Although enteroviruses may be found in faeces, they are not associated with gastroenteritis. The
other viruses are associated with gastroenteritis.
False HTLV-1
True Hantaviruses
False Poliomyelitis
True Rabies
Comments Influenza A H5N1 is otherwise known as the "bird flu" It afflicted Hong Kong in late 1997 and was
transmitted from poultry to humans. Hantaviruses is transmitted from rodents to humans and rabies
can be transmitted from various mammals to humans.
False Hepatitis A
True Hepatitis B
True Hepatitis C
False Hepatitis E
Comments A chronic carrier state can exist in hepatitis B, C and Delta Virus infections
False Rubella
True HIV
False Measles
True HBV
True HCV
Comments HIV, HBV and HCV are transmitted by blood. Rubella and measles are transmitted via respiratory
droplets
25. The following may be useful for prognostic purposes in HIV-infected individuals
Comments HIV envelope antibody remains high throughout the course of HIV infections and thus does not
carry any prognostic value. HIV-p24 antigen had been widely used in the past as a prognostic marker
but it has been superseded by HIV viral load. CD4 count plays an important role in telling us what
stage the disease is at.
26. The following markers are usually present in a hepatitis B carrier with
chronic active hepatitis
True HbsAg
False HbsAb
True HBV-DNA
True HbeAg
Comments In patients with chronic active hepatitis, HBV replication is present and hence HBV-DNA and
HbeAg. HbsAg is present. Anti-HbcIgM is normally absent although it may occasionally be
detected during periods of exacerbations.
True Enteroviruses are one of the commonest causes of CNS infections in childhood
False Electron microscopy of the cerebrospinal fluid (CSF) is a useful diagnostic test
Comments Meningitis usually occurs together with encephalitis. Enteroviruses are one of the commonest
causes of CNS infections in childhood. Electron microscopy is too insensitive to be useful as a
diagnostic test. PCR assays for HSV are now routinely used for the diagnosis of herpes simplex
encephalitis. The detection of antibody in the CSF is a useful diagnostic marker
False The rash caused by parvovirus B19 is due to the presence of virus in the skin
True Herpes simplex virus (HSV) infection is usually diagnosed by viral culture
Comments Varicella-zoster virus (VZV) infection may cause a vesicular rash. The rash caused by
parvovirus B19 is probably an allergic reaction due to the deposition of immune complexes.
Measles is usually diagnosed by serology. Herpes simplex virus (HSV) infection is usually
diagnosed by viral culture. VZV can be grown in cell culture although this is rarely used for
diagnosis.
False Can have serious side effects when occurring in a woman in the third trimester of pregnancy
True May be acquired by having close contact with an infant with congenital rubella syndrome
Comments Rubella infection can be asymptomatic. It may be indistinguishable clinically from parvovirus
B19 infection. The most serious effects occur when a pregnant woman is infected in the first
trimester of pregnancy. It is usually preventable by vaccination. Infants with congenital rubella
syndrome pose a great infectious risk since they may excrete virus in great quantities up to 1
year of age.
Comments Rubella immunity may be determined by an enzyme immunoassay. CFT is not commonly used
for the diagnosis of rubella infection; Haemagglutination-inhibition tests are. IgM antibody is
usually detectable after the onset of rash Rubella virus can be cultured although this is rarely
used as a method of diagnosis. Pre-natal diagnosis of rubella in a foetus such as amniocentesis
involve some degree of risk to the fetus.
False HSV-2
True EBV
True HCV
False Adenoviruses
True HHV-8
HSV-2 was associated with genital cancers at one time but this has now been disproved. EBV is
associated with Burkitt's lymphoma, nasopharyngeal carcinoma, and large-cell lymphomas in
immunocompromised individuals. Although adenoviruses may be oncogenic in vitro, there is no
evidence that they are associated with human cancers. HHV-8 is associated with Kaposi's
sarcoma.
True HSV-2
True Rubella
True HIV
True HBV
True CMV
All the above viruses may cause congenital infection. Rubella and CMV are usually transmitted
transplacentally in utero, whilst HIV, HBV and HSV-2 are usually transmitted perinatally
during the birth process.
True HIV
False Parvovirus
True HBV
False CMV
True HTLV-1
HIV and HTLV-1 are retroviruses. HBV is a DNA virus whose replication depend on a reverse
transcriptase
True Herpseviruses
False Poxviruses
False Picornaviruses
True Orthomyxoviruses
False Paramyxoviruses
All DNA viruses, with the exception of poxviruses replicate in the nucleus. All RNA viruses,
with the exception of orthomyxoviruses, replicate in the cytoplasm
False Rubella
True Rabies
True Hantaviruses
Zoonoses are infections which are transmitted from a vertebrate animal to humans either
directly, or indirectly through an arthropod vector. Rabies may be transmitted to human from
various mammals. Hantaviruses and Lassa fever from rodents. The reservoir of Japanese
encephalitis is in birds and pigs and it is transmitted to humans via culex mosquitoes
False HSV-1
False HBV
False HIV
True Rotaviruses
False Contain
True Have long incubation periods
False Rabies
"Slow virus infections" refer to viral or prion infections of the brain characterised by a long
incubation period which is in terms of years. Human prion diseases, Subacute Scelerosing
Panecephalitis (SSPE), and Progressive multifocal leucoencephalopathy (PML) are considered
as slow virus infections. Herpes Simplex Encephalitis and rabies are not.
True Encephalitis
True Gingivostomatitis
False Shingles
HSV-1 infection may result in encephalitis, gingivostomatitis, genital herpes, and corneal ulcers.
Shingles arise from a previous infection by VZV
False Parvovirus
True HSV-1
True VZV
HHV-8 is associated with Kaposi's sarcoma, HTLV-1 is associated with Adult T-cell lymphoma
Parvovirus is associated with fifth disease, HHV-6 with Roseala Infantum, EBV with
Nasopharyngeal Carcinoma and oral leukoplakia
19. The following viruses are thought to cause gastroenteritis
False Enteroviruses
True Caliciviruses
True Astroviruses
False Paramyxoviruses
Although enteroviruses may be found in the faeces, they are not associated with gastroenteritis.
Paramyxoviruses are not associated with gastroenteritis
True Human polyoma virus JC is associated with progressive multifocal leucoencephalopathy (PML)
True HPV-6 and HPV-11 are associated with Juvenile laryngeal papillomatosis
True Patients with Epidermodysplasia verruciformis are susceptible to widespread warts caused by
HPV
Human papillomaviruses (HPV) are associated with warts. HPV-16 and 18 are associated with
genital cancers. Patients with Epidermodysplasia verruciformis are susceptible to widespread
warts caused by a variety of HPV types. These lesions may become cancerous later on in life.
Only influenza A is known to undergo antigenic shift. Both influenza A and influenza B may
undergo antigenic drift. Only influenza A respond to amantidine
RSV mainly causes disease in infants. It is the main cause of bronchiolitis. There is no vaccine
available. It will not respond to amantidine but will respond to ribavirin. It is not known to cause
latent infections,
True Acyclovir
HIV may respond to reverse transcriptase inhibitors, many of which are nucleoside analogues. It
may also respond to HIV protease inhibitors which are the most potent agents to date.
Neuraminidase inhibitors are used for the treatment of influenza virus infections whilst acyclovir
is used for herpesvirus infections.
False HIV is mainly integrated into the genome of long-lived cell populations
During the incubation period, there is a huge amount of viral replication and consequently a high
turnover of CD4 cells. Only a very small proportion of HIV is integrated into the genome of long-
lived cell populations. The viral load has a direct bearing on the prognosis. HIV envelope
antibody remains at a high level throughout the incubation period.
HAV infection does not result in chronic infection and therefore cirrhosis. It may be prevented by
the administration of human normal immunoglobulin (HNIG), and active vaccination by an
inactivated vaccine. By the time jaundice appears, there is little viral excretion and thus
infectivity.
HBV infection may result in chronic infection, cirrhosis of the liver and hepatocellular
carcinoma. The presence of HbeAg indicates viral replication and thus infectivity. Chronic
HBV infection may respond to interferon therapy.
27. The following statements are true of hepatitis B core escape mutants
Hepatitis B core escape mutants are associated with acute fulminant hepatitis. They are anti-
HbeAg antibody positive and HBV-DNA positive. HbsAg is present and therefore they
should be no anti-HbsAg antibody.
HCV is mainly transmitted by blood. It may cause chronic infection and is associated with
hepatocellular carcinoma. It may respond to interferon therapy. There are a number of HCV
genotypes.
True Cases of dengue haemorrhagic fever usually result from reinfection by a different serotype of
dengue virus
Dengue virus infection result from bites by Aedes mosquitoes. Cases of dengue
haemorrhagic fever usually result from reinfection by a different serotype of dengue virus: an
immunopathological mechanism is thought to be involved. No animal reservoir is involved in
the vast majority of dengue cases: transmission arise from a man-mosquito-man cycle.
Yellow fever is not found in Asia, it is prevalent in some West African and Central and South
American countries. It can be prevented by vaccination with a live attenuated vaccine.
Measles virus infection may result in pneumonia and encephalitis. There is one stable
serotype. Measles will not respond to acyclovir but can be prevented by vaccination.
1.6 Which of below mentionned structures on microbes are recognised by human immune
system molecules.
A. MHC I molekuly
B. MHC II molekuly
C. PAMP x
D. PRR
E. SGR
2.1 Synthetic molecule that bind the receptors on B cells, but does not stimulise their
production of specific antibodies unless it is conjugated with bigger immunogenic molecules is
A. adjuvans
B. carrier
C. hapten x
D. immunogen
E. tolerogen
2.2 Which of below mentioned molecules induce the strongest specific immune reaction in man
A. 250 000 Da self plasmatic protein
B. 150 000 Da toxin produced by bacterium x
C. 500 Da plasmatic protein from monkey
D. 400 Da molecule from foreign man
E. 200 Da molecule of carbohydrate that is the same in all biological species
2.3 During early stages of lymphocyte development the receptor-epitop binding can results in
inactivation or death of the lymphocyte, or unresponsivness. The epitop is
A. hapten
B. immunogen
C. tolerogen x
D. carrier
E. adjuvans
2.4 Natural killers induce lysis of infected cell, that has the decreased exposition of MHC
I molecules. NK cells do this by activation of receptors:
A. receptors for complement
B. Fc receptors
C. KAR – killer activation receptors x
D. KIR – killer inhibition receptros
E. TCR
2.5 Antibodies mediated accumulation of macrophages in the site of pathogen arises thanks to
A. Receptors for complement
B. Fc receptors x
C. KIR – killer activation receptors
D. PRR – pattern recognition receptors
E. TLR – toll like receptors
2.7 Binding of epitop on Fab portion of molecule before its Fc fragment anchors in target
structure is not required for
A. molecule of the carrier
B. conjugate of hapten-carrier
C. hapten
D. IgE x
E. IgG
4.1 Which of mentioned cells are present on the place of infection by worms
A. basofils
B. eosinofils x
C. lymphocytes
D. monocytes
E. neutrofils
4.3 In microscopic smear of the pus from bacterial infection site there will be mostly present
A. bazofils
B. eozinofils
C. lymphocytes
D. monocytes
E. neutrofils x
4.5 Which of mentionned cells engulf extracellular bacterial antigens and are not antigen
presentig cells
A. basofils
B. dendritic cells
C. eosinofils
D. macrophages
E. neutrofils x
4.8 Predominant form of leucocytes in the blood of patient with longlasting pyogenic bacterial
infection are
A. B lymphocytes
B. Juvenil and inmature neutrofils x
C. Monocytes a macrophages
D. Natural killers
E. T lymphocytes
5.4 Which of below mentionned molecules are expressed on the surfaces of human infected
cells or abnormal cells and are recognised as stress molecules by NK cells
A. Alfa or beta defensines
B. C3 convertase and properdine
C. Cytokines and chemokines
D. Interferon and/or interferon beta
E. MICA and MICB x
6.5 In patient with allergy the cross binding of the allegen molecule with Fab fragment of
immunoglobulin will activate degranulation of mediators:
A. IgA
B. IgE x
C. IgG
D. Histamin
E. Mastocytes
6.10 Migration of B lymfocyte to a specific place (e.g. lymphatic node) depend on production of
A. antibodies
B. CD8 molecule
C. CD3 molecule
D. complement
E. Selectins x
8.2 Existence of BCR and TCR specific only for one antigen is enabled by:
A. rearrangemet of genes for variable part of immunoglobulin molecule x
B. maturation of immunity
C. exclusion of allels
D. Isotype swich
E. Junction diversity
8.3 Presence of only mother´s or only father´s genes for V chains is enabled by
A. Exclusion of allels x
B. Antibody diversity
C. Isotype switch
D. Junction diversity
E. Random rearrangement of VD and VDJ
8.5 During restimulations of memory B cells the minor mutation in DNA encoding variable part
of light and heavy chains will result in
A. Isotype swich
B. Decrease of antigenic specificity
C. Exclusion of allels
D. Specificity maturation
E. .apoptosis of the cell x
9.1 Which of problems is typical for patients with aplasia of tymus
A. problems of B cells development only
B. inactivation of complement system
C. dysfunction of NK cells
D. dysfunction of cellular immunity
E. dysfunction in T lymphocyte differenciation and B cell mediated immunity x
<h6>10.1 T cells recognise epitop to which they are exposed for the first time by</h6>
A. randomly generated TCRs x
B. MHC molecule
C. Ig molecule
D. One of many molecule on the surfacei
E. PRR
<h6>10.6 Presence of lipopolysaccharid from bacterial cell wall in the tissue stimulates
(nonspecific stimulation)</h6>
A. APC cells to cytokines secretion
B. Release of cytokines to activate leucocytes
C. Stimulation and sectretion of IFN- g that activate leucocytes
D. Th0 cells differentiate to Th1 cells
E. All answere are correct x
11.1 Production of of visible aggregates to identify blood groupes by binding IgM with apropriate
A or B antigens is:
A. aglutination x
B. complement aktivation
C. neutralisation
D. opsonisation
E. precipitation
11.2 C3b is able to bind on a microbe that will make it more attractive for phagocytosis. This
process is
A. agglutination
B. complement activation
C. neutralisation
D. opsonisation x
E. precipitation
11.3 Test (diffusion in agar) that identifies production on not soluble complexes of soluble
antigen with antibody is the example of
A. agglutination
B. complement activation
C. neutralisation
D. opsonisation
E. precipitation x
11.4 Antigen antibody binding that will result in incapability of microbe to bind on target cells is:
A. agglutination
B. complement activation
C. neutralisation x
D. opsonisation
E. precipitation
11.5 Which of mentione izotypes of antbodies activate complemet cascade if bound to antigen
A. IgA and IgD
B. IgA and IgE
C. IgA and IgM
D. IgE and IgG
E. IgG and IgM x
12.2 Which of mentioned cells are the source of the 2 nd signal to T cell after immunological
synapsis is produced
A. APC x
B. Anergised T cell
C. CD4+T cells
D. B cells
E. Naive T cells-thymocytes
12.3 Which of mentioned cells need cooperation of pMHC with costimulatory 2nd signals from
APC to be activated.
A. anergised T cells
B. B cells
C. Mastocytes
D. Naïve T cells x
E. Natural killers
13.4 Whicb of mechanisms are/is responsible for repeated infection with influenza virus
A. Neutralisation antibodies are short living
B. Low stimulation of CD4+ T cells to produce memory cells
C. Intracellular localisation disable stimulation of immune system
D. Hypersensitivity type 1 is present only in second exposition
E. Variability of viral antigens x
13.6 Molecule of acute phase of infection synthesised in liver during bacterial and viral infection
is
A. CRP x
B. Chemokin
C. Complement
D. Immunoglobulin
E. Interleukin
13.9 Even unvaccinated child can be protected against some of vaccination preventable
diseases because of
A. Herd immunity x
B. Genetical predisposition
C. Antigenic shift
D. Imunity escape
E. Tolerance
14.2 Patient with history of PNC allergy was given the dose in PNC injection. In minutes the
symptoms of hypotension, tachycardia, diffuse exanthema arised. She suffered from:
A. Anaphylactic reaction x
B. Anergy
C. ADCC
D. Ashtma
E. Contact hypersensitivity
14.3 Which of mentione reaction is induced by interaction of host cell membrane with IgM or IgG
antibodies
A. Arthus reaction
B. Serum disease
C. hypersenzitivity I type
D. hypersensitivity II type x
E. hypersensitivity IV type
14.4 A patient with known allergy ate unconsciously the cake with peanuts that resulted in diffus
exanthema, respiratory distress and diffuse lung symptoms This is typical for:
A. hypersensitivity I. Type x
B. arthus reaction
C. serum disease
D. IgG binding on extraceluluar matrix in respiratory tract mucous membrane
E. IgM dependent disease from immunocomplexes
16. 3 Inativation and destructon of lymphocytes bearing the BCR or TCR recognising self
molecules results in
A. autoimmunity
B. positive selection
C. negative selection x
D. tolerance
E. supression
16.4 Molecular mimicry is resulting from reaction against self structures produced by
A. cross reacting antibodies x
B. loss of toleranie
C. loss of negative selection
D. loss of anergy of immunologicall priviledged organs
E. loss of central tolerance
1. In vaccination against variola – small pox – the attenuated annimal virus was used to prevent
human virus infection (antigenic similarity and cross reacting immunity). Similar principle is used in
vaccination against
A. tuberculosis
B. tetanus
C. measles
D. poliomyelitis
E. pertussis
1. Substance that resemble toxin, that has its antigenic and immunogenic properties but not
toxic properties is called
A. antitoxin
B. toxoid x
C. toxin
D. antibody
E. hapten
2. Complete antigen:
F. hapten
G. semihapten
H. imunogen x
3. CH50 is a
A. Specific immunity function
B. Nonspecific immunity function x
6. Titer is
A. Dilution of serum x
B. Indicating the amount of antibodies in not diluted serum
C. For ex. 1:124
D. Indicate the number of vial in witch the reaction is positive
E. Is to detect precipitation
F. Is used to detect nonspecific immunity
12. Vaccination can produce autoimmune disease., is not needed because the disease against that
they protect are eradicated – no more present of the world. These statements are
A. true – based on immunological and epidemiological studies
B. correct - based on immunological surveys
C. flase and presented by antivaccination activitists
D. are not well studied and that is why they can be tolerated
E. are well studied and should be propagated
15. CH50 is a
F. detection of hemolytical activity of complement x
G. Specific immunity function qualitative test
H. Nonspecific immunity function qualitative test
i. Titer is
I. Dilution of serum
J. Indicating the amount of antibodies in not diluted serum x
K. For ex. 1:124
L. Indicate the number of vial in witch the reaction is positive
M. Is to detect precipitation
N. Is used to detect nonspecific immunity
17. To detect soluble molecules – toxin for ex. Which test can be use:
A. agglutination
B. precipitation x
C. diffusion
D. B and C is correct
18. Which of mentioned way is correct to indicate the titer of antigodies:
A. 16O
B. 1: 16O x
C. 1 x16O
12. Exotoxín
a) Is immunogenic x
b) Is the synonyme to toxoid
c) Is neutralised by anatoxin
d) Is a part of vaccine against tetanus
16. Specific IgM antibodies in serum from the blood taken from umbilical cord is indicating
a) Intrauterine infection of the fetus
b) Transplacentarly transmitted antibodies from mother
c) Perinatal infection x
d) Postnatal infection