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  • (2010) Biodegradation of Caffeine by Pseudomonas Sp. NCIM 5235 PDF

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    Chaitanya
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    [2010]Biodegradation of caffeine by Pseudomonas sp. NCIM 5235.pdf

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    13 vizualizări9 pagini

    (2010) Biodegradation of Caffeine by Pseudomonas Sp. NCIM 5235 PDF

    Încărcat de

    Chaitanya

    Drepturi de autor:

    © All Rights Reserved
    Descărcați ca PDF sau citiți online pe Scribd
    Indicator pentru conținut neadecvat
    din 9
    Research Journal of Microbiology 5 (8) 2010 ISSN 1816-4935 / DOL: 10.39234jm.2006.115.123 © 2010 Academie Journal In. Biodegradation of Caffeine by Pseudomonas sp. NCIM $238* ‘Swati Sucharita Dash and Sathyanarayana N. Gummadi Department of Biotechnology, Indian Institute of Technalogy-Madsas, ‘Chermai 600 136, India AbstractsA bacterial stain capable of ufliing caffeine asthe sole source of carbon and nitrogen has been isolsted from sol of coffee plantation area and deposited st National Collection of industri Microorganisms as Pseudomonas sp. based on biochemical studies In this study, the isolate showed close resemblance to Pseudomonas pusida strain T-S7 based on 16S sRNA analysis. The ability of Pseudomonas sp. to degrade caffeine was studied by growing the isolate in eaffeine medium with 1.2, 5, 7.5 and 10 g L~' of initial ‘concentration of caffeine, [twas cbcerved that te isolate could eficiently degrade caffeine tan initial conosntrtion of S g L~! within 48 h It could also bring about 599 and 21 ons : 10 : 5 02 : : 10 46 40 om ox 019 ons aa? we Ols 086 eas 7 sa ost 0% OL rn il oa 0% ols 4508 art es O17 oe Zs om 036 ‘The eapertnan was epee Uvice an he vlis repress he mean of We illzen pena wie and devia of +580 a The cai ibis concretion of eine a which He cell goth cease ws fund tobe20 gL _Basilovirio gp. Tames Ilana (AY 0941151) Pseudomonas sp. DSY-42 (AF 3160371) L__. Prendomenas putida KCTC1639 (AY 7508591) ‘Preudomonas pda sain op (AS276648.1) Peendamonas putida ssi op (AI 276650.1) Peendomonaspsida (U S238.1) Peeudomonas pia soan A29 (AY 5800711) Peeudomonas putida E57 (AB 188054.1) Peendomonas sp. NCIM 5235° ‘Soyabean acter EN 189 avs0n.1) Fig. 1: Phylogenetic tree diagram based on 16S sRNA analysis using CLUSTALY software. Numbers in parentheses indicate Gentank EMBL /DDBI database accession mumbers.* indicates the isolated stain in this study 1, 3elimethybxanthine (heophylline) is formed in lesser amount, Hence degradation through ‘thoophyiline route canbe considered as the mincr pathway of degradation (Blecher and Lingens, 1977). Im order to investigate the route of caffeine degradation by Pseudomonas sp. NCIM 5238 the metabolite profiles were obtained ot various fime points during the course of degradation and ‘computed with the proiles ofthe standards (Fig, 2). It was observed that degradation of eufTeine by 749 Res. J. Microbiol, 5 (9): 745-753, 2010 ie 12001 ‘Renton time (ais) Fig. 2: Metabolites fomed at various time points during degradation of eaeine by Pseudomonas 9p. NCIMS235 when the initial concentration is Sg L~'. ‘The analysis was performed by RP-HPLC on Zorbax C18 column with 10 mM arnmenium phosphate: acetonitrile 1:4 as mobile phase, Peak 1 corresponds to caffeine at 4.9 min, Peak 2 corresponds to 3, 7- imethyixanthine at 3.5 min, Peak 3 corresponds to 7-methylsanthine at 3.0 min, Peak 4 corresponds to xanthine at 2.8 min Pseudomonas 5p. NCIM $235 follows the demethyation pathway with the formation of 3, 7- dimethylsanthine and then subsequent derivatives (Fig, 2). At 24 h of degradation both

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