Experiment No: 01

Results:

Figure1: White DNA strands were appeared.

Experiment No: 02
Discussion:

Results: DNA sample was visualized using 0.8 % agarose gel for validation.

Discussion:
As opposed to independently adding small volumes of reagents to each individual tube, prepared
a master mix solution in a 1.5 ml eppendorf tube. This contains all reagents except the template
DNA. Using the master mix will increase accuracy, reduce reagent loss on tips and reduce tube-
to-tube variability.