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The road of discovery and application of yeast
Technological Function of Yeast
Synthetic Biology • Prospect of the
synthesis of entire
genomes for
Systems Biology
construction of
“ideal” industrial • Dough Leavening (baker’s yeast)
yeast
• Brewing (brewer’s yeast)
• Exploitation of biological complexity
Molecular Biology of yeast strain improvement for • Distilled alcohol beverages fermentation
example to optimise yeast cell
process. (distiller’s yeast)
Genetics
• Metabolic engineering of yeast by directed genetic
modifications and reconstruction to improve • Wine fermentation (wine yeast)
performance of industrial strains
Biochemistry
• Genetics engineering of yeast by gene cloning and
• Other alcoholic fermentation (ragi)
trnasformation of specific genes for a new desirav=ble
characteristics
– Indonesia: tape, brem
Microbiology
• Genetics breeding • Dairy fermentation (kefir grain)
• Mutagenesis and selection
• Clonal selection • Soy fermentation (soy sauce, tauco)
• Isolation and Identification Adapted from Verstrepen et al. 2006
Yeast Technology in Food Industry Yeast Technology in Food Industry
Jakarta September 4, 2007 Jakarta September 4, 2007
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Desirable properties of Distiller’s
Wine Yeast
Yeast
• Controlled fermentation started in 20th century
• Natural fermentation: any number of • Natural fermentation involves different yeast
species may be involved species such grow succesively at initial.
• Controlled fermentation: S.cerevisiae – In the most active fermentation stage to end
of fermentation: Saccharomyces cerevisiae
– Very alcoholic tolerant and gives a good yield
– Ferments rapidly • Desirable characteristics:
– Good flavor production – Rapid fermentation rate
– Tolerance to SO2
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Storage Stability of Active Dry Baker’s Yeast Ragi and Kefir grain
in Variuos Atmosphere
Atmosphere Residual Activity (%)*)
• Ragi • Kefir grain
Nitrogen 79.5
– Mixed yeast culture – Mixed yeast culture
Carbon dioxide 81.9
– Present together with – Present together
Carbon monoxide 85.2
amylolytic molds with lactic acid
Argon 80.7
bacteria
Hydrogen 88.9
Air 43.3
Vacuum 70.3
*) after storage for 3 d at 55oC
Hill, 1987
Trade fermentation
PACKAGING
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Baker’s yeast fermentation Processing aids and additives to
improve activity of Dry Yeast
• Added to yeast cream or press cake prior
• Substrate: to drying:
– Molasses: beet and/or cane – Swelling agent:
– Other: whey • Methyl cellulose or carboxymethyl cellulose (1-2%
of yeast solid) (Langejan, 1980)
• Fermentation condition:
– To reduce solid leaching
– Aerobic • Monoglycerides, soya lecithin, glycerol polyesters
and sorbitan esters (Hill, 1987)
– To improve storage in air:
• Addition antioxidant
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Product definition Product definition
• Yeast Extract: • Yeast Autolysates/Autolysed yeast:
– Yeast extract comprises the water soluble – Autolysed yeast is the concentrated, not
components of the yeast cell, the composiiton extracted, partially soluble digest obtained
of which is primarily amino-acids, peptides, from food-grade yeast. Solubilisation is
carbohydrates and salts. Yeast extract is accomplisehd by enzyme hydrolysis or
produced through the hydrolyses of peptide autolysis of yeast cells. Autolysed yeast
bonds by the naturally occurring enzymes contains both soluble and insoluble
present in edible yeast or by the addition of components derived from the whole yeast
food grade enzymes cells.
The Food Chemical Codex The Food Chemical Codex
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Production Process of Yeast
Source of yeast cream
Extract Absent in
production of
Yeast cream Clarification yeast • Conventional:
autolysate
– Baker’s Yeast
Plasmolyses Concentration – Brewer’s Yeast
• Alternative:
Liquid: 50-65% dry matters • Candida utilis
Autolyses Paste: 70-80% dry matters
• Kluyveromyces marxianus
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Other yeast-derived products
• Colorants
– Pigmented yeasts such as Rhodotorula, Thank you
Phodospondium, Cryptococcus,
Sporidiobolus, Sporobolomyces
• Yeast Polysaccharides:
– Food application and nutraceutical potential
– Potential species S. cerevisiae, Pichia holstii,
Hansenula sp, Candidia, Rhodotorula Further information:
lilis@seafast.org
• Yeast Enzymes Http://www.seafast.ipb.ac.id