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SAFETY SUMMARY
The warning labels shown below are attached on the Model L-8900 high-speed amino acid analyzer.
Read the warning labels carefully, and check the instructions on them to acquire a clear understanding with reference to actual parts.
Periodically check the appearances of these warning labels to see if they are clean to allow easy reading over a safe distance.
If any one of the warning labels becomes illegible due to deterioration, contact you’re nearest Hitachi High-Technologies Corporation sales representative and service office of Hitachi High-Technologies
Corporation sales representative for replacement with a new one.
WARNING CAUTION
BIOLOGICAL HAZARD HOT COLUMN OVEN
Sticking of waste solution to your body Can cause burns.
can cause contagion.
The column oven is hot.
Don’ t touch a sample without protective Before accessing the column oven
device. for column replacement, etc., make
If waste solution sticks to your body, sure the temperature indication
on displayed is lower than 55℃
wash it off immediately and disinfect the
affected area, then consult with a physician. P/N 852-8323
P/N 852-8320
CAUTION
WARNING HOT REACTOR
BIOLOGICAL HAZARD Can cause burns.
Sticking of waste solution to your body The reactor is hot.
can cause contagion. Before accessing the reactor
for column replacement, etc., make
Don’ t touch a sample without sure the temperature indication
protective gloves. on displayed is lower than 55℃
If waste solution sticks to your body, P/N 852-8324
wash it off immediately and disinfect the
affected area, then consult with a physician.
P/N 852-8321 CAUTION
HOT COLUMN AND COVER
Can cause burns.
WARNING
The light source lamp and its cover
BURSTING are hot.
Can cause injury if a sample Before lamp exchange, wait 20 minutes
Container (made of glass or after turning off instrument power
polyethylene) bursts due to cracks. supply and make sure the lamp and
its cover are cooled.
Before applying pressure to a sample P/N 852-8322
container by means of N2 gas, check
for cracks and be sure to put the
container in the protective device.
P/N 852-8325 SAFETY - 18
6.2.1
6-5
6.2.2
Bubbles produced
in the pump valve. Open the drain valve for purging.
Pump unit (common) Contamination in pump Disassemble and wash the inlet valve, For washing method, refer to para. 6.3.1 or 6.3.5.
valve (seal chips) outlet valve and pump head.
Service life of Replace the pump seal For replacing pump seal, refer to 6.3.1.
pump seal expired (service life: approx. 1 month).
Reagent bottle inside filters
(common) Filter clogged
(adhesion of Wash the filter by ultrasonic cleaner. For replacing the filter, refer to 6.3.4.
reagent Otherwise, replace the filter.
Filter contaminant)
Pressure in pumps 1
(or 2) is insufficient or
fluctuates. Service life of
Injection valve of auto
sampler (pump 1 only) rotor seal expired For replacing the rotor seal, refer to 6.3.9.
Replace the rotor seal.
Bubbles produced Open the drain valve to perform purge. When starting the operation of the system after it has not been used for a long time (1 week or
in pump valve more), be sure to purge the pump because bubbles must have been generated naturally in the
Check if the pump pressure
pump unit.
Seal chips in pump value fluctuates by 1 MPa Disassemble and wash the inlet valve, outlet 1. Wash the pump head (with the inlet valve and outlet valve mounted) by ultrasonic cleaner
Pump unit valve and pump head.
valve or more on the monitor screen. (with distilled water).
Service life of seal 2. If the inlet valve and outlet valve cannot be washed enough in step 1 above, remove them
Replace the seal (service life: 1 month). from the pump head and wash them by the ultrasonic cleaner (with neutral detergent). For
expired (liquid leak)
details and cautions, refer to 6.3.1.
Line filter Clogging with 1. The clogged line filter may increase pressure in the buffer pump. Wash and replace it
impurities in periodically (every 6 months).
sample or injection Remove the line filter from the filter unit and
wash it by the ultrasonic cleaner. 2. For preventing the line filter and column from being clogged, be sure to feed sample through
port seal chips of a 0.45 µm filter.
auto sampler
Photometer lamp
Check of running time Replace when the running time of 1000 hours
Degradation is reached or exceeded. For replacing the lamp, refer to 6.3.6.
(Maintenance screen of the CRT)
6-6
6.2.3
Drift (including waveness) Flow path connection Liquid Visually check and retighten the liquid Use a spanner for further tightening the connections employing setscrews. Other connections
leak leaking section. including teflon tube, in particular, should be tightened with hand. (Or, the teflon tube inside is
a a b
crushed to cause clogging.)
Operate pump 1 and pump 2 in this order and then
Insufficient warm-up wait 30 minutes or more (B-1 for pump 1 and R1 for 1. For preventing sample impurities from being accumulated, feed sample solution through a
a b pump 2 in 50%:50%). 0.45 µm filter in addition to pretreatment. (Avoid analyzing
b a sample with white suspension or precipitate.) Buffer
Accumulated impurities and contamination Regenerate and smoothen the column. 2. For extracting resin of an faulty column from the
Analytical column Elution of impurities
and contamination
column, connect the column so that its flowing
c
c Re-pack or replace the column. direction is reversed. Feed liquid into the pump Pump 1
to eliminate contamination.
Baseline floating Column
Buffer solution Make sure that there is no NH3 generated both at the
Entered NH3 Replace the buffer solution.
a location of installing the system and the storage for reagent solutions.
a
Gap
NH3 filter column Since the gap is caused by pressure rise in pump 1, prevent the flow path from being clogged,
If there is gap, repack or replace the column. referring to the para. of abnormal pressure, 6.2.1.
b
b
Degraded ninhydrin solution Check the CH2 chromatogram according to the para. of degraded sensitivity, and replace the solution if degraded.
Coloring of If the peak height of Asp - Glu is lower than For reference, normal Pro/Asp (peak height ratio) is 3.0 or less in the standard assay.
CH2 (440 nm) ninhydrin solution Data in Data in that of Pro, ninhydrin solution is degraded.
chromatogram degraded normal abnormal Replace the ninhydrin solutions (R1, R2).
condition condition
Sample impurities and Impurities and If there is impurity or contamination, repack or For preventing sample impurities from being accumulated, filter sample through a 0.45 µm filter in
Analytical column flow path contamination contamination replace the column. addition to pretreatment. (Avoid analyzing a sample with white suspension or precipitate.)
Separation degraded accumulated in the
column, or gap in Since the gap is caused by pressure rinse in pump 1, prevent the flow path from being clogged,
column Gap If there is gap, repack or replace the column.
referring to the para. of abnormal pressure, 6.2.1.
Column temperature Check the chromatogram with the analytical
and buffer changeover conditions (peak moving table) in the Refer to para. 4.1.7 for protein hydrolyzates assay and 4.2.7 for phisiological fluid assay in the
Analyzing program timing (phisiological instruction manual and change program instruction manual.
fluid assay) appropriately.
Coloring of ninhydrin Check the CH2 chromatogram according to the para. of For preventing degradation of ninhydrin solution, be sure to pressurize the reagent bottle with nitrogen
degraded degraded sensitivity, and replace the solution if degraded. gas even after completion of analysis.
Degraded reproducibility of
peak position Unstable flowrate in pump 1 Take remedy in accordance with the para. of
“Pressure in both pumps 1 and 2 is
insufficient or fluctuates”.
Liquid leak from flow path
6-7
N2 gas
IN Soft nylon tube
Regulator
Manifold(4W)
OUT
1.0TT M2 IN
IN IN
NC 1.0TT Pressure sensor
Drain
Drain
IN
1.0TT
1.0TT OUT OUT
N2 gas unit
2.5VT
IN 10
2.5VT
1 9 2.5VT
2 8
2.5VT 2.5VT
1.0TT
1.0TT
APPENDIX3. FLOW PATH
Manifold(10W) 3 7
4 2.5VT
M2 2.5VT 6
5
Washing Buffer Buffer Buffer Buffer Buffer Buffer MIN MIN Distilled
solution solution solution solution solution solution 2.5VT solution reagent reagent water
2.5VT
C-1 B-1 B-2 B-3 B-4 B-5 B-6 R-1 R-2 R-3
1.0TT
1.0TT
1.0TT
1.0TT
1.0TT
1.0TT
1.0TT
1.0TT
1.5TT
1.0TT
1L 1L 1L 1L 1L 1L 1L 1L 1L 1L
1.5TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT
Degasser
deaerator
tube
300µL 470µL 470µL 470µL 470µL 470µL 470µL 470µL 470µL 470µL
Solenoid
B-1 B-2 B-3 B-4 B-5 B-6 R-1 R-2 R-3
valve
1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT 1.0TT
2 Line filter
0.5TT 1 2µm
Filter Column oven
5µm
1.0TT
Drain Preheat coil
1mL/min Drain 0.25ST×0.3m
0.5TT
Pump unit P1
Separation column
Φ4.6×60L
packing material
#2622SC
1 2
Injection valve
Joint 0.8ST
0.5ST 6 3
Anmonia column 1 Mixer
Φ4.6×40L
Sample loop packing material
4 #2650L 3
5
2
0.17ST
Preheat coil
Washing
3 0.25ST
port
4
2
Syringe valve Reaction
Needle column(2)
5 1 Φ4.6×40
Sample
Injection tube
Drain
1.0TT
Syringe Joint
flow cell
Autosampler unit Φ1.3×6mm
0.8TT 0.8TT volume
0.8TT 8µL(black quartz) PD
P1 P2 0.33TT×3m PC(data processingz)
Pump head washing mechanism