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Item No : 22.11
Semester – III:
BBTDBT302/
MIBDBT302 Biochemistry 5-0-0-5
BBTDBT304/
MIBDBT304 Biochemistry Laboratory 0-0-3-2
Total 22-3-6-29
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3-1-0-4
UNIT - II 15 Periods
COMPLEX DIFFRENTIATION
Complex integration –Line and Surface Integrals- Cauchy’s integral theorem – Integral formula –
Taylor’s and Laurent’s series (without proof) – Singularities – Zeros – Poles and
residues – Cauchy’s residue theorem – Contour integration – evaluation of integrals of the type
only.
UNIT - IV 15 Periods
FOURIER TRANSFORMS
The infinite Fourier transforms – Sine and cosine transforms – properties – Inversion theorem –
problems – Convolution theorem – Parseval’s identity – Problems – Finite Fourier transforms – Sine
and cosine transforms – Evaluation of definite Integrals-problems Solving boundary value problems
using finite Fourier sine and cosine transforms.
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TEXTBOOKS
REFERENCES
2. “Advanced engineering Mathematics,” 8/e, J. Erwin Kreyszig, Wiley eastern Ltd., 2007.
2006.
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4-0-0-4
Unit – I 12 Periods
Unit – II 12 Periods
Different stages of Cell cycle, G1, S, G2 and M.Mitosis - Prophase, Prometaphase, Metaphase,
Anaphase, Telophase and Cytokinesis; Meiosis - The stages of Meiosis, Significance of
Meiosis. Comparison of Mitosis and Meiosis, Cell Cycle Check Points
Unit – IV 12 Periods
Unit V 12 Periods
CellSignaling
Basic Elements of cell signalling systems.Autocrine, paracrine and endocrinesignalling. Second
messengers and their role in signal transduction :CAMP, IP3. Cell Surface receptors in signal
transduction, GPCR and Tyrosine kinase receptors.
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TEXT BOOK
1. “Cell & Molecular Biology – Concepts & Experiments,” 6/e, Gerald Karp, John Wiley & Sons,
USA, 2007.
2. Molecular Biology of the Cell, 5/e, B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts and
P. Walter, Garland Science, Taylor & Francis, USA, 2008.
REFERENCE BOOK
1. “Molecular Cell Biology,” 5/e, J. Darnell, H. Lodish& D. Baltimore. W.H. Freeman, USA,
2003.
2. “Molecular Biology: Genes to proteins,” 3/e, B.E. Tropp, D. Freifelder, Jones & Bartlett
Publishers, USA, 2008.
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4-0-0-4
A brief outline of processes for the production of some commercially important organic Jacids:
citric acid, itaconic acid, lactic acid, acetic acid, gluconic acid.
Amino acids: glutamic acid, lysine, aspartic acid, phenylalanine. Alcohols: ethanol, 2,3-
butanediol.
UNIT - II 15 Periods
PRODUCTION OF SECONDARY METABOLITES
UNIT - IV 15 Periods
Specialty Bio-products for the Agricultural, Food & Pharmaceutical Industries
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TEXTBOOKS
REFERENCES
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4-1-0-4
UNIT - I 15 Periods
ABSORPTION & EMISSION SPECTROSCOPY
UV-visible Spectroscopy
Types of electronic transitions; Instrumentation; Beer-Lambert’s law; deviation from Beer’s law;
Applicability of Beer’s law to solutions of proteins, nucleic acids and cell cultures. Qualitative &
Quantitative analysis of biological samples (including enzyme assay)
FTIR spectroscopy
Spectrofluorimetry
UNIT - II 15 Periods
SPIN RESONANCE AND MASS SPECTROMETRY
NMR spectroscopy
1
H NMR Spectroscopy: Principle; Instrumentation; Chemical shift, spin-spin coupling and
coupling constant; shielding & de-shielding. Applications in the field of biotechnology: structure
elucidation of simple molecules, quantitative analysis; 13C NMR spectroscopy; 2D NMR - Its
significance for protein studies. Introduction to solid-state NMR spectroscopy.
ESR Spectroscopy
Mass Spectrometry
Principle- Instrumentation- Parent ion peak- Nitrogen rule- Molecular weight determination.
MALDI-TOF.
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UNIT - III 15 Periods
CHROMATOGRAPHY
UNIT - IV 15 Periods
ELECTROPHORESIS
Zeta potential measurement (principle, electric field induced denaturation of proteins during
measurement and the importance of diffusion barrier).
TRACER TECHNIQUES
TEXTBOOKS
1. “Instrumental Methods of Analysis” 6/e, Willard, Merritt, Dean & Settle, CBS Publishers,
Delhi, 1986.
2. “Biophysical Chemistry” 1/e A. Upadhyay, K. Uadhyay& N. Nath, Himalaya Publishing
house, Mumbai (Only for Units III and IV)
REFERENCES
1. “Principles & techniques of practical biochemistry”, 5/e, Keith Wilson &John Walker,
Cambridge University Press, New York, 1994
2. “Modern Spectroscopy”, 4/e, J. Michael Hollas, John Wiley & Sons Inc., USA, 2005
3. “Modern Analytical Techniques”, David T. Harvey, Tata McGraw Hill Co., India, 2000
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4-0-0-4 BBTCBT304/MIBCBT304:BASIC BIOINFORMATICS
UNIT I 14 Periods
The central dogma – genome- the human genome project and the need for databases and
annotation ,Databases – contents, structure and annotation: file formats; annotated sequence
databases; Retrieval of biological data: data retrieval with Entrez& DBGET/LinkDB; data
retrieval with SRS (sequence retrieval system).
Concept of sequencing -DNA, RNA & proteins- Next Generation Sequencing and data handling,
interpreting sequence data
UNIT II 15 Periods
Sequence similarity searches; similarity and identity among biological sequences -amino acid
substitution matrices; FASTA& BLAST; sequence filters; iterative database searches & PSI-
BLAST-Multiple-sequence alignment, local and global alignment algorithms- multiple-sequence
alignment and family relationships
UNIT IV 13 Periods
Process of drug discovery- drug design and virtual screening – structure and receptor based
ligand design – docking – scoring –small molecular libraries – lead optimisation
TEXTBOOK :
1. “Bioinformatics –Sequence and Genome Analysis’ David W.Mount, CSHL Press, New
York 2e 2005
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5-0-0-5 BBTDBT301/MIBDBT301: MICROBIOLOGY
UNIT - I 15 Periods
INTRODUCTION TO MICROBIOLOGY
History and Scope, bacterial cell structure and function, physical and chemical control of
microbes. Study of microbial structures, microscopy and specimen preparation- Light, Phase
contrast, Epi Fluorescent, Confocal Microscopy, SEM, TEM and AFM.
UNIT - II 15 Periods
MICROBIAL METABOLISM, GENETICS & FUNGI
Microbial Nutrition, Overview of Microbial Metabolism: Anaerobic Respiration and Fermentation,
Outline of metabolic Pathway involved in synthesis of various classes of antibiotics, Microbial
Genetics: An overview of the mechanisms of Genetic Variations.
UNIT - IV 15 Periods
MICROBIAL PATHOGENECITY AND THEIR CONTROL
Microbial Pathogenesis and Immunity: Host- parasitic relationships, Immunity: Types, cells
involved. Pathogenesis of bacterial diseases, Microbial mechanism for escaping host
defenses.Pathogenicity of some important human diseases- HIV, Tuberculosis, Candidiasis.
UNIT - V 15 Periods
APPLIED MICROBIOLOGY: Microbial Enzymes, SCP, Biofuels, Bio-polymer, Bioremediation,
Biofouling, Biosensors, Production of Bio-gas, Biofertilizers, Biopesticides, Probiotics and
Biofilms of Clinical importance: Cystic Fibrosis, Wound infections, Biofilms on Implants.
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TEXTBOOKS
“Microbiology” 5/e, L.M. Prescott, J.P. Harley, D.A. Klein, WCB McGraw Hill, New York, USA,
2002.
REFERENCES
1. “Foundations in Microbiology”, 4/e, K. Talaro, A. Talaro, W.C. McGraw Hill, New York, USA,
2002.
4. “Environmental microbiology”, 2/e, R.M. Maier, I.L. Pepper, C.P. Gerba, Academic Press,
CA USA, 2009.
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5-0-0-5 BBTDBT302/MIBDBT302: BIOCHEMISTRY
UNIT - I 15 Periods
BIOMOLECULES
Lipids - Structure and functions of fatty acids, triacylglycerols, phospholipids and glycolipids.
UNIT - II 15 Periods
ENZYMES
Nucleic acids metabolism - Purines and Pyrimidines- synthesis, degradation and its control.
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UNIT - IV 16 Periods
AMINO ACID AND LIPID METABOLISM
UNIT-V 14 Periods
Electron transport chain and its complexes; Oxidative phosphorylation: chemiosmotic theory;
Proton motive force, ATP synthesis - rotational catalysis; Uncouplers of oxidative
phosphorylation; control of ATP production.
TEXTBOOKS
REFERENCES
1. “Biochemistry,” 4/e, Donald Voet and Judith G. Voet,John Wiley and Sons, 2011.
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0-0-3-2 BBTDBT302/MIBDBT302: BASIC BIOINFORMATICS LAB
1. Internet basics: URL naming & addressing, effective usage of search engines
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0-0-3-2 BBTDBT303/MIBDBT303: MICROBIOLOGY LAB
2. Culturing Microbes
i) Pour Plate
5. Identification of Microorganisms
a) Staining Techniques
i) Simple ii) Gram Stain iii) Negative Stain iv) Acid Fast Stain v) Endospore Stain
b) Motility Determination
c) Biochemical Testing
d) Hydrolytic Reactions
8) Quantitation of Microorganisms
i) Counting:
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iv) Turbidometry
TEXT BOOK
REFERENCE BOOK
1. “Microbial Applications,” 8/e, Harold J. Benson, McGraw Hill, New York, USA, 2001.
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0-0-3-2 BIBDBT304/MIBDBT3044: BIOCHEMISTRY LABORATORY
7. Estimation of DNA.
8. Estimation of RNA.
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Scheme of study for
B.Tech. (Biotechnology)
Semester – IV
BBTDBT402/
MIBDBT402 Biochemistry 5-0-0-5
BBTDBT404/
MIBDBT404 Biochemistry Laboratory 0-0-3-2
Total 20-5-6-29
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3-1-0-4 BBTCBT401/ MIBCBT401: ENGINEERING MATHEMATICS - IV
UNIT - I 15 Periods
FOURIER SERIES
Introduction – Dirichlet’s conditions – Euler’s Formula – General Fourier series – Odd and even
functions – Half range series – Parseval’s identity – Complex form of Fourier series – Root –
mean square (RMS) value of a function – Numerical methods of finding Fourier coefficients -
Harmonic analysis.
UNIT - II 15 Periods
PARTIAL DIFFERENTIAL EQUATIONS (PDEs)
One dimensional wave equation–Assumptions- Boundary and initial value problems – Fourier
series solution - One dimensional heat equation –Assumptions- Steady and unsteady states -
Boundary and initial value problems– Fourier series solution.
UNIT - IV 15 Periods
TWO DIMENSIONAL HEAT FLOW EQUATIONS
Two dimensional heat flow equation –Assumptions- Steady state heat flow in two dimensions –
Laplace equation in Cartesian and Polar coordinates (including annulus) – Fourier series
solution.
TEXTBOOKS
1.“Engineering Mathematics Vol – III,” edition, Dr.P.Kandasamyet al.,S.Chand& Co., India, year.
REFERENCES
1. “Advanced Engineering Mathematics,” 8/e, Erwin Kreyszig, Wiley Eastern Company, 2005.
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3-1-0-4 BBTCBT402/ MIBCBT402: FLUID MECHANICS FOR PROCESS ENGINEERS
UNIT - I 15 Periods
UNIT - II 15 Periods
FLUID KINEMATICS
Concept of boundary layer: Boundary layer theory, form drag, skin drag, Drag coefficient
Laminar and turbulent flow through closed conduit; velocity profiles and friction factor for smooth
and rough pipes. Head losses due to friction in pipes, non-circular sections, fittings, etc.;
introduction to compressible flow.
Flow around solids and through packed beds; Drag curves for regular and irregular solids.
Flow through fixed bed: Pressure drop, flooding and loading, friction factor for packed beds,
Ergun's equation.
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UNIT - IV 15 Periods
FLOW MEASUREMENT, PUMPS, FANS AND COMPRESSORS
Measurement of fluid flow; Orifice meter, Venturi meter; Pitot tube; Rotameter, Weirs and
Notches. Wet-gas meter, Hot-wire and hot-film anemometers. Principles and applications of
TEXTBOOKS
REFERENCES
1. “Fluid Mechanics for Chemical Engineers,” 3/e, N. De Nevers, McGraw Hills, New York,
2004.
2. “A Textbook of Fluid Mechanics & Hydraulic Machines,” 9/e, R.K.Bhansal, Laxmi Publication,
India, 2010.
3. “Chemical Engineering”, 1/e J. M. Coulson & Richardson, Vol. 1, Asian Book Pvt. Ltd, New
Delhi, 2001.
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4-0-0-4 BBTCBT403/ MIBCBT403: BIO PROCESS CALCULATIONS
UNIT - I 15 Periods
UNITS &DIMENSIONS AND PVT RELATIONSHIP:
Basic & derived units; use of mole in calculations; methods of expressing compositions of
mixtures & solutions.Gas systems: ideal- & real-gas laws; gas constant; calculations of
pressure, volume & temperature, using ideal-gas law; use of partial pressure & partial volume of
a pure component in gas calculations. P-V-T relationship for real gases; law of corresponding
states; Equation of states approach to the P-V-T relationship of non-ideal gas; compressibility
factor.
UNIT - II 15 Periods
MATERIAL BALANCE IN DOWNSTREAM PROCESSING
Application of material balance to unit operations like distillation, evaporation, crystallization &
drying.
Material balance with chemical reaction; limiting & excess reactants; recycle, bypass & purging;
problems on recycle, bypass & purging.
Humidity & saturation: calculation of absolute humidity; molal humidity; relative humidity &
percentage humidity; use of humidity in condensation & drying; humidity chart.
Stoichiometry of cell growth & product formation – elemental balances; degrees of reduction of
substrate & biomass; available-electron balances; yield coefficients of biomass & product
formation; maintenance coefficients, problems based on yield coefficients, respiration quotients,
& reduction ratio.
UNIT - IV 15 Periods
ENERGY BALANCE IN BIO PROCESS ENGINEERING
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TEXT BOOKS
1.“Stoichiometry,” 3/e, B. I. Bhatt and S. M. Vora, Tata McGraw-Hill, New Delhi, 1996.
2. “Bio process Engineering Principles,” 2/e, Paulin M Doran, Acadamic press, NewDelhi, 2006.
REFERENCES
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4-1-0-4 BBTCBT404/ MIBCBT404: BIOCHEMICAL THERMODYNAMICS
UNIT - I 15 Periods
BASIC CONCEPTS AND LAWS OF THERMODYNAMICS:
Brief review of System, Surroundings and Processes, Open and Closed systems, State
properties, Intensive andExtensive Properties, State and Path functions, equilibrium state and
Phase Rule, Zeroth Law of Thermodynamics,Reversible and Irreversible processes, General
Statement of First and Second and Third laws of thermodynamics. (Abrief review of the above is
desired with simple numerical problems).
UNIT - II 15 Periods
THERMODYNAMIC PROPERTIES OF PURE FLUIDS
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UNIT - IV 15 Periods
PHASE EQUILIBRIA
Criteria of phase equilibria, criterion of stability: phase equilibria in single and multi-component
systems, phase rule fornon-reacting systems, Duhem’s theorem, Vapour-Liquid Equilibria, VLE
in ideal and Non-ideal solutions, Non-Idealsolutions: azeotropes, Consistency test for VLE data,
calculation ofactivity coefficients using Gibbs-Duhem equation, Liquid-Liquid equilibrium
diagrams, Liquid phase reactions,heterogeneous reaction equilibrium, solid-solid equilibria.
Numerical examples.
UNIT - IV 15 Periods
CHEMICAL AND BIO CHEMICAL REACTION EQUILIBRIA
TEXTBOOKS
3. “Bio process Engineering Principles,” 2/e, Paulin M. Doran, Acadamic press, NewDelhi,
2006.
REFERENCES
1. “Chemical and Process Thermodynamics,” 2/e,B.G. Kyle, Prentice-Hall of India, New Delhi,
2000.
2. “An Introduction to Thermodynamics,” 1/e., Y. V. C. Rao, New Age International, India, 2000.
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3-0-0-3 BBTCBT405/ MIBCBT405: BIOCHEMICAL REACTION ENGINEERING
UNIT - I 15 Periods
REACTION KINETICS
UNIT - II 15 Periods
HOMOGENEOUS REACTIONS AND IDEAL REACTORS
Introduction to reactor design.Single ideal reactor.Batch reactor, plug-flow reactor & mixed-flow
reactor for single reactions.
UNIT - IV 15 Periods
HETEROGENOUS NON-CATALYTIC REACTIONS
Industrial non-catalytic reactions, non-catalytic reaction models for explaining diffusion kinetics
and ash control steps, design aspects of fluid-particle reactor, Fluid-Fluid reactions - Kinetics
and design.
TEXTBOOKS
1. “Chemical Reaction Engineering,” 3/e, O. Levenspiel, John Wiley & Sons, New York, 1999.
2. “Advances in chemical Engineering - volume 17”, 1/e, James Wei, John L. Anderson,
Kennath B. Bischoff, John H. Seinfeld, Academic press, New York, 1994.
REFERENCES
1.“Chemical Engineering Kinetics,” 3/e, J. M. Smith, McGraw-Hill International, New York, 1981.
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5-0-0-5 BBTDBT401/ MIBDBT401: MICROBIOLOGY
UNIT - I 15 Periods
INTRODUCTION TO MICROBIOLOGY
History and Scope, bacterial cell structure and function, physical and chemical control of
microbes. Study of microbial structures, microscopy and specimen preparation- Light, Phase
contrast, Epi Fluorescent, Confocal Microscopy, SEM, TEM and AFM.
UNIT - II 15 Periods
MICROBIAL METABOLISM, GENETICS & FUNGI
Microbial Nutrition, Overview of Microbial Metabolism: Anaerobic Respiration and Fermentation,
Outline of metabolic Pathway involved in synthesis of various classes of antibiotics, Microbial
Genetics: An overview of the mechanisms of Genetic Variations.
UNIT - IV 15 Periods
MICROBIAL PATHOGENECITY AND THEIR CONTROL
Microbial Pathogenesis and Immunity: Host- parasitic relationships, Immunity: Types, cells
involved. Pathogenesis of bacterial diseases, Microbial mechanism for escaping host
defenses.Pathogenicity of some important human diseases- HIV, Tuberculosis, Candidiasis.
UNIT - V 15 Periods
APPLIED MICROBIOLOGY: Microbial Enzymes, SCP, Biofuels, Bio-polymer, Bioremediation,
Biofouling, Biosensors, Production of Bio-gas, Biofertilizers, Biopesticides, Probiotics and
Biofilms of Clinical importance: Cystic Fibrosis, Wound infections, Biofilms on Implants.
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TEXTBOOKS
1. “Microbiology” 5/e, L.M. Prescott, J.P. Harley, D.A. Klein, WCB McGrawHill, New York,
USA, 2002.
REFERENCES
1. “Foundations in Microbiology”, 4/e, K. Talaro, A. Talaro, W.C. McGraw Hill, New York,
USA,2002.
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5-0-0-5 BBTDBT402/ MIBDBT402: BIOCHEMISTRY
UNIT - I 15 Periods
BIOMOLECULES
Lipids - Structure and functions of fatty acids, triacylglycerols, phospholipids and glycolipids.
UNIT - II 15 Periods
ENZYMES
Nucleic acids metabolism - Purines and Pyrimidines- synthesis, degradation and its control.
UNIT - IV 16 Periods
AMINO ACID AND LIPID METABOLISM
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UNIT-V 14 Periods
Electron transport chain and its complexes; Oxidative phosphorylation: chemiosmotic theory;
Proton motive force, ATP synthesis - rotational catalysis; Uncouplers of oxidative
phosphorylation; control of ATP production.
TEXTBOOKS
REFERENCES
1. “Biochemistry,” 4/e, Donald Voet and Judith G. Voet,John Wiley and Sons, 2011.
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0-0-3-2 BBTCB406/ MIBCBT406: FLUID MECHANICS LABORATORY
LIST OF EXPERIMENTS
1. Pipe friction
2. Flow through fittings and valves
3. Calibration of venturi-meter
4. Calibration of orifice-meter
5. Efflux time
6. Single stage Centrifugal pump – performance characteristics
7. Reciprocating pump – performance characteristics
8. Gear pump - performance characteristics
9. Non-Newtonian flow
10. V-notch
11. Pressure drop in helical coil
12. Annular flow
13. Packed bed
14. Fluidised bed
15. Drag study
16. Manometer dynamics
17. Dynamics of interacting and non-interacting systems
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0-0-3-2 BBTDBT403/ MIBDBT403: MICROBIOLOGY LAB
2. Culturing Microbes
i) Pour Plate
5. Identification of Microorganisms
a) Staining Techniques
i) Simple ii) Gram Stain iii) Negative Stain iv) Acid Fast Stain v) Endospore Stain
b) Motility Determination
c) Biochemical Testing
d) Hydrolytic Reactions
8) Quantitation of Microorganisms
i) Counting:
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iii) Haemocytometric count of yeast cells
iv) Turbidometry
TEXT BOOK
REFERENCE BOOK
2. “Microbial Applications,” 8/e, Harold J. Benson, McGraw Hill, New York, USA, 2001.
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BBTDBT404/ MIBDBT404: BIOCHEMISTRY LABORATORY
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