Rofp jLp pTkfbg

(Mushroom Spawn production)

 Rofp jLp pTkfbg
(Mushroom Spawn production)

 vfWffGg jfnL pTkfbg ug{sf] nflu jLpsf] cfjZoQmf eP h:t} Rofp

jLp pTkfbg ug{nfO{ klg jLpsf] cfjZoQmf kb{5 . Rofpsf] :kf]/ g}
o;sf] vf; jLp xf] t/ o;jf6 jLp oTkfbg ug{ k|fljlws b[li6sf]0fn]
pko'Qm b]lv+b}g . :kf]/sf] ;f6f] Rofpsf] /];fjf6 - Mycelium _
jLp ptkfbg ug{ ;lhnf] x'g hfG5 . jLp pTkfbg ubf{
Mycelium nfO{ ux'Fsf] jLp tyf cGo ljleGg dfWodx?df a9fO{
jLpsf] ?kdf k|of]u ul/G5 . Rofpsf] jLpnfO{ c+u|]hLdf Spawn
elgG5 .
Rofpsf] jLp nfO{ ltg j6f steps df pTkfbg
ug]{ ul/G5
!=z'4 jLp -pure culture _ / o;sf] ;Djw{g
@=dfp jLp pTkfbg - Mother spawn _
#=Jofj;foLs jLp -5g]{ jLp_ pTkfbg - planting
spawn _
 !=z'4 jLp -pure culture _

 Rofpsf] z'4jLp cg';Gwfg s]Gb| tyf 7'nf Jofj;foLx?n] w]/} nufgLdf

pTkfbg ub{5g\ .
 ;fgf nufgLstf{nfO{ z'4 jLp pTkfbg ug{ jl9 vlr{nf] x'g] ePsf]n] o:tf
Jofj;foL x?n] 7'nf Jofj;foLx?jf6 jf ;+sng s]Gb|jf6 jLp k|fKt ug'{kb{5 .
 ;+sng a]Gb|jf6 pknJw jLpnfO{ ljleGg sNr/ ldl8ofx?df ;Djw{g ug'{
kb{5 .
 Rofpsf] hft cg';f/ /];fnfO{ ;Djw{g u/L /fVgsf] nflu lk=l8=P= -potato
Dextrose Agar _ jf Pd=O=P= -Malt Extact Agar _ jf l;=O=P= -
Compost Extract agar _ sf] k|of]u ug]{ ul/G5 .
@= Dffp jLp pTkfbg - Mother Spawn
production

 Dffp jLp pTkfbg ug{sf] nflu pure culture nfO{ klxnf k]l6«Kn]6df
x'sf{pg' kb{5 . o;sf] nflu k]l6«Kn]6nfO[{ !*) l8=;]=df @ #306f jf
c6f]Sn]edf !@!l8=;] tfqmddf @) ldg6 ;Dd lgd{lns/0f ug]{ .
 rlkª /8 tyf l:6n lg8n cflbnfO{ klg lgd{lns/0f ul/ ;f]sf]
;xfotfn] Rofpsf] /;fnfO{ sdfl6 lg8nsf] ;xfotfn] cu/Kn]6df
;fl/G5 .
 Rofpsf] /];f ;fbf{ laminar flow sf] k|of]u ug'{ kb{5 .
qmdz
 Dffp jLp pTkfbg ug{sf] nflu pure culture nfO{ klxnf k]l6«Kn]6df
x'sf{pg' kb{5 . o;sf] nflu k]l6«Kn]6nfO[{ !*) l8=;]=df @–# 306f jf
c6f]Sn]edf !@! l8=;] tfqmddf @) ldg6 ;Dd lgd{lns/0f ug]{ .
 rlkª /8 tyf l:6n lg8n cflbnfO{ klg lgd{lns/0f ul/ ;f]sf]
;xfotfn] Rofpsf] /;fnfO{ sfl6 lg8nsf] ;xfotfn] cu/Kn]6df ;fl/G5
.
 Rofpsf] /];f ;fbf{ laminar flow hood sf] k|of]u ug'{ kb{5 .
jLpsf] nflu pko'Qm dfWod (Substrate)

 dfWod jgfpg rflxg] cfjZos ;fduLx? M–

Rofpsf] jLp -Mother culture_ ,ux'Fsf] jLp , lk=l8=P=,
k]6«LKn]6, skf;,ux'F ksfpg] efF8f], bfljnf] ,PNo"ldlgod kmf]On,
Sofnl;od sfjf]{g]6, lhK;d, Knfl:6s y}nf, c6f]Sn]e, n]ldgf/
km|nf] x'8, rlkª /8 / l:6n lg9n cflb .
 dfWod (Substrate) sf] tof/L M–

 Rofpsf] /];f pTkfbg ug{sf] nflu ux'Fsf] bfgfnfO{ dfWod -substate _ sf]
?kdf k|of]u ul/G5 .
 ux'FnfO{ /fd|f]l;t s]nfO{,w'nf] ,d}nf,6's|f 6's|L tyf sfl6Psf] bfgfnfO{ x6fpg] .
 ;kmf ux'nfO{ /ftel/ lr;f] kfgLdf lehfpg] / ef]nLkN6 !)–!% ldg6 ;Dd
pl;Gg] . jLpnfO{ cfwf dfq kfSg] ul/ pl;Gg -ux'FnfO{ km'6g glbg] _ .
 cfwf kfs]sf] ux'nfO{ efF8fjf6 t'/Gt lemls lr;f] kfgLn] /fd|f]l;t @–$
k6s kvfnL kfgL tsf{pg] / ;kmf sk8fdf 5fofdf ;'sfpg] .
 ux'F rnfpgsf] nflu sf7sf] bfljnf]sf] k|of]u ug]{ .
qmdz
 jLpdf cln cln lr:ofg /xg] cj:yf x'Fb}df Sofnl;od sfjf]{g]6 – ^ u|f= /
lhK;d – !@ u|f=k|lt lsnf] jLpsf] b/n] ld;fO[ /fVg] / ;j} bfgfdf kg]{ ul/
ldnfpb} hfg] .
 ux'Fsf] bfgfsf] lk=Pr= &=) /fVg] .
 lgd{nLs/0f ug{sf] nflu ^–(OGrsf] Knfl:6ssf y}nfx?df k|lt Knfl:6s @))
u|f=sf] b/n] ux'sf] jLp eg]{ / To;sf] d'vnfO{ kfgL g;f]:g] ul/ skf;n] jGb
u/L PNo"ldlgod kmf]Pnn] 9fSg] .
 cj ux'F e/]sf] Knfl:6s y}nf]nfO{ c6f]Sn]edf !@! l8=;]= tfks|ddf Ps 306f
;Dd jf c6f]Sn]edf @–$ l;l6 nfUg] j]nf;Dd lgd{lns/0fn ug]{ .
qmdz
 c6f]Sn]esf] k|];/ ;'Godf em/kl5 y}nfx?nfO{ jflx/ lgsfnL lr;f] kfg]{ .
 jf]tn jf Knfl:6s y}nf lr;f] ePkl5 Laminar flow hood df /flv
Inoculation ul/G5 .
 /fd|f] Rofpsf] /];f ePsf] k]6LKn]6jf6 Inoculation ug{sf] nflu #–$ j6f
Rofpsf] /];fsf] 6'sf|x? chopping rod sf] ;xfotfn] sfl6 cu/ ;lxtsf]
k]6«LKn]6df hLjf0f'/lxt jftfj/0fdf ;fl/G5 .
 k]6«LKn]6df ePsf] Rofpsf] /];fnfO{ y}nfdf ;f/]kl5 ux'F;+u ldnfpg #–$
k6s ;Dd xNnfpg' kb{5 .
qmdz
 /fd|f] Rofpsf] /];f ePsf] k]6LKn]6jf6 Inoculation ug{sf] nflu #–$ j6f
Rofpsf] /];fsf] 6'sf|x? chopping rod sf] ;xfotfn] sfl6 cu/ ;lxtsf]
k]6«LKn]6df hLjf0f'/lxt jftfj/0fdf ;fl/G5 .
 k]6«LKn]6df ePsf] Rofpsf] /];fnfO{ y}nfdf ;f/]kl5 ux'F;+u ldnfpg #–$
k6s ;Dd xNnfpg' kb{5 .
#= Jofj;foLs jLp -5g]{_ pTkfbg (Planting
Spawn)M–

 Jofj;foLs jLpsf] nflu klg ux'Fsf] dfWod - substrate _ jgfpg] tl/sf –

dfp jLpdf h:t} ul/G5 .
 ux'Fsf] dfWod jgfO{ ;s]kl5 inoculation ug{ k]6«LKn]6sf] /];fo'Qm cu/sf]
dfp jLpsf] k\of]u ul/G5 .
 Pp6f Knfl:6s y}nf jLpnfO{ inoculation ug{ @–# rDrf dfp jLpsf]
cfjZoQmf kb{5
 Inoculation ubf{ chopping rod , ;'O/f] / rDdr hlxn] klg
lgd{lns/0f u/]sf] x'g k5{ / dfp jLpsf] y}nf / 5g]{ jLpsf] y}nfnfO{ d'v
hf]8]/ lj:tf/} dfp ljhgsf] y}nfjf6 @–# rDdr /];f ;lxtsf] ux'F ;fg' k5{ .
qmdz
 jLp ;fg]{ sfo{ Laminar flow hood df hLjf0f'/lxt jftfj/0fdf ug'{
kb{5
 To;kl5 y}nfx?nfO{ skf;sf] ljsf]{ nufO{ PNo"ldlgod kmf]Onn] ljsf]{ 9fsL
/j/n] jfFWg' kb{5 / incubation df /fVg' kb{5 .
 jLpnfO{ incubation df /fVbf Knfl:6s y}nfdf jLpsf] hft , cf]yf/f] u/]sf]
ldlt n]v]/ /fVg' kb{5 .
 Ps kf]sf dfp jLpn] @)–@% kf]sf tof/ ug{ ;lsG5 .
 o;/L jLp cf]yf/f] /fv]sf] @–# xKtf leqdf Rofpsf] /];f ;j} ux'Fdf km}lng
hfG5 / Rofp ljs|L ug{ of]Uo x'G5 .
 jLpsf] e08f/0f M–

 Rofp jLpnfO{ tof/ ePkl5 t'/Gt} k|of]u ug{ pko'Qm x'G5 .

 o;nfO{ e08f/0f ug{ @–$ l8=;]=tfks|d ;j} eGbf pQd
dflgG5 / o;nfO{ dlxgf lbg;Dd e08f/0f ug{ ;lsG5 .
 jLp KoflsË tyf 9'jfgL M–

 Rofp jLpnfO{ lbgdf eGbf /ftdf 9'jfgL ug{ pko'Qm

x'G5 .jLp KoflsË ug{ k"j{ skf;sf] ljsf]{ l7s 7fs 5
5}g r]s ug'{ kb{5 / jLpnfO{ sf6'{gdf KoflsË
u/]/ dfq 9'jfgL ug' kb{5 .
s]lx kmf]6f]x?
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