Detailed experiments of Phytophthora blight disease of pigeonpea

Experiment No. PB- 001-10

Title: Collection, purification and maintenance of pure couture of Pdc.

Completed
Repetition: Yes
 Experiment No. PB- 002-10

Title: Single spore culture and pathogenecity of Pdc isolates

Objective: To prove the pathogenecity of all the isolate collected form different location

Methods and materials:

Inoculation technique
ar spray
drench

Inoculum form

3. Host- Growth stage: 15 days old

Variety- HY3C

Environment: Controlled environment (Growth chamber-

Temp- 15 & 25)
Design of experiment
Treatments: 6+1
Replication: 3
No. of plants/ pot
Observation
6.1. Temp: Max & min
RH
Light
6.2. Progress of disease development
0 day- inoculation
1 day- incubation
Typical symptoms development
6.3. Termination of the experiment
≥ 80% plant showing the typical PB symptoms
6.4. Precautions:
Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.

7. Data analysis: Collection compilation and statistical analysis

with graphs, dendograms ete.

Conclusion: Whether it needs repetition- Yes

Time line: Spredding of suspension of Pdc on 2% agar:

8.07.10
no.of isolates: 5
 Pickup spores:
9.07.10

Spredding of suspension of Pdc on 2% agar: 12.07.10

no.of isolates: 5
Pickup spores: 13.07.10

Spredding of suspension of Pdc on 2% agar: 14.07.10

no.of isolates: 5
Pickup spores: 15.07.10

Spredding of suspension of Pdc on 2% agar: 19.07.10

no.of isolates: 5
Pickup spores: 20.07.10

Spredding of suspension of Pdc on 2% agar: 21.07.10

No. of isolates: 5
Pickup spores: 22.07.10

Experiment No. PB- 003-10

Title: Evaluation of inoculation techniques and PB development

Objective: To identify the most effective inoculation technique for PB development.

Methods and materials:

1. Inoculation technique
1.1. Scattered inoculum on soil
1.2. Soil mixing
1.3. Soil drenching
1.4. Stem bit staging/ attaching
1.5. Stem bit insertion into the soil near root zone
1.6. Stem scratching/scrapping and applying inoculum
1.6.1. Stem bit attachment
1.6.2. Cotton swabbing
1.6.3. Inoculum brushing on scrapped portion
 1.7. Leaf scar
1.8. Toothpick

2. Inoculum form: Mass multiplied on grains

Stem bits (Plant debris) of Pdc 14
3. Host- Growth stage: 10 days and 30 days old plants grown on sterile red soil
Varity- HY3C
4. Environment: Controlled environment Growth chamber- Temp- 25
Greenhouse- temp-28
5. Design of experiment
Treatments: 10+1
Replication: 3
No. of plants/ pot:5
6. Observation
6.1. Temp: Max & min
RH
Light
6.1.2. Progress of disease development
0 day- inoculation
1 day- incubation
Typical symptoms development
6.1.3. Termination of the experiment
≥ 80% plant showing the typical PB symptoms
6.1.4. Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.

7. Data analysis: Collection compilation and statistical analysis with graphs,

dendograms ete.

8. Conclusion: Whether it needs repetition- Yes with Pdc14 single sporangial culture

Time line:
6 methods repetition
Date of sowing and inoculum multiplication on grains: 5th july
Date of inoculation: 15.07.10

Scattered inoculum on soil

Soil mixing
Soil drenching
Stem bit staging/ attaching
Stem bit insertion into the soil near root zone

Date of observations: 16.07.10 onwards

 Effect of different debris inoculation in pigeon pea
Date of inoculation: 29.06.10 Isolate: Pdc 14
Environmental condition: Growth chamber Date of sowing: 22.05.10
Age of seedlings: 38 days
Inoculation Replication Total Observations
methods plants
Date/No. of infected plants Symptoms Incidence of the disease Severity of the Plant parts
disease infected
30.6 1.7 2.7 5.7 SR stem Wilting 30 1 2 5 30 1 2 5 Leaf Stem
5 4 0 1 0 2 defo 80 0 20 0 3 4 4 8 Upp
lesio liati er
R1 n on leaf
Debris mix R2 5 2 0 0 3 40 0 0 60 3 3 3 6 Upp
in soil er
leaf
R3 6 6 0 0 0 defo 100 0 0 0 4 5 6 8 Upp
liati er
on leaf
R4 5 2 2 1 0 40 40 20 0 3 3 4 7 Upp
er
leaf
Control R1 12 0 0 0 0 0 0 0 0 0 0 0 0

Debris R1 5 0 0 0 0 0 0 0 0 0 0 0 0
Combined
to the stem R2 5 0 0 0 1 0 0 0 20 0 0 0 0

R3 5 0 0 0 0 0 0 0 0 0 0 0 0

R4 5 0 0 0 0 0 0 0 0 0 0 0 0

Control R1 12 0 0 0 0 0 0 0 0 0 0 0 0
 Effect of different debris inoculation in pigeon pea
Date of inoculation: 29.06.10 Isolate: Pdc 14
Environmental condition: Greenhouse Date of sowing:22.05.10
Age of seedlings: 38 days
Inoculation Replication Total Observations
methods plants
Date/No. of infected Symptoms Incidence of the disease Severity of the Plant parts
plants disease infected
30/ 1/ 2/ 5/ SR Wilting WSL 30/6 1/7 2/7 5/7 30/6 1/ 2/ 5/ Leaf Stem
6 7 7 7 7 7 7
5 4 0 1 0 def S 80 0 20 0 3 5 7 8 Upp
olie L er
R1 tion 1 leaf
Debris mix R2 5 4 1 0 0 S 80 20 0 0 3 4 5 8
in soil L
3
R3 5 5 0 0 0 Sl 100 0 0 0 5 7 9 9
3
R4 5 4 0 0 1 S 80 0 0 20 4 5 6 8
L
2
Control R17 7 0 0 0 0 0 0 0 0 0 0 0 0

Debris R1 5 0 0 0 0 0 0 0 0 0 0 0 0
Combined
to the stem R2 5 0 0 0 0 0 0 0 0 0 0 0 0

R3 5 0 0 0 0 0 0 0 0 0 0 0 0

R4 5 0 0 0 0 0 0 0 0 0 0 0 0

Control R1 10 0 0 0 0 0 0 0 0 0 0 0 0
 Data sheet for Effect of Pdc inoculums (Grain) on pigeon pea (HC3Y) in Green house

Date of raising seedling: 14.6.10 Age of seedling: 10 days

Date of inoculation: 24.06.10 Date of observation:25th on wards

Isolat Inoculati Replic Total IP Observations

e on ation plants
methods
Pdc24 Date/No. of infected plants Symptoms Incidence of the disease Severity of the disease Plant parts
infected
25.6 26.6 27.6 28.6 SR Wilting WSL Leaf Stem
13 24 48 72 96 - 8 2 3 1 8, 2, 3 61.5 15.3 23.0 23.0 3 5 8 √

R1
Inoculums R2 14 - 5 4 5 1 5,4,3 35.7 28.5 35.7 35.7 3 4 9 √
scattered
on soil R3 8 - 5 2 1 5,2,1 62.5 62.5 12.5 12.5 4 5 8 √
surface
R4 12 - 1 1 10 7 1 8.33 8.33 8.33 83.3 2 2 8 √

Mean

Inoculums R1 12 2 8 2 - 2 1,6,3 1 16.6 66.6 16.6 0 2 3 6 7 √

mixed in
soil R2 12 2 6 3 - 4,4 2,1 16.6 50.0 25.0 0 3 3 6 7 √

R3 15 1 7 6 1 7,6,1 6.66 46.6 40.0 6.66 2 4 6 8 √

R4 12 1 5 5 - 5,6 1 8.33 41.6 41.6 0 2 3 6 7 √

Mean

Harvest R1 9 1 7 - - 1,7,1 11.1 77.7 0 0 2 4 6 8 √

mycelium
/sporangia R2 14 4 5 4 - 5,4,4 28.5 28.5 28.5 0 3 4 4 5 √
from grain
& soil R3 13 2 5 5 - 4,6 1,2 15.3 38.4 38.4 0 2 4 6 8 √
drench
R4 14 - 4 8 2 4,2,5 3 0 28.5 57.1 14.8 3 4 4 7 √

Mean
 Data sheet for Effect of Pdc inoculums (Grain) on pigeon pea (HC3Y) in Green house

Date of raising seedling: 14.6.10 Age of seedling: 10 days

Date of inoculation: 24.06.10 Date of observation: 25th on wards
Isolat Inoculati Replic Total IP (hrs) Observations
e on ation plants
methods
Pdc14 Date/No. of infected plants Symptoms Incidence of the disease Severity of the disease Plant parts
infected
25.6 26.6 27.6 28.6 SR Wilting WSL Leaf Stem
5 24 48 72 96 - 1 - 1 1,1 0 20.0 0 20.0 2 2 √

R1
Inoculums R2 9 - 1 2 1 1,2,1 0 11.1 22.2 11.1 2 2 2 √
scattered
on soil R3 12 1 - - - 1 8.33 0 0 0 2 2 √
surface
R4 14 - - - - 0 0 0 0 √

Control R1 17 - - - - 0 0 0 0

R2 16

Inoculums R1 12 5 1 5 1 2,1,5 3,1 41.6 8.33 41.6 8.33 2 3 7 8 √

mixed in
soil R2 14 1 2 5 2 2,5,2 1 7.14 14.2 35.7 14.2 3 3 4 5 √

R3 13 4 9 - - 2 2,9 2 30.7 69.2 0 0 4 4 6 8 √ √

R4 14 4 9 - - 2 2,5 2 28.5 64.2 0 0 5 6 7 8 √ √

Control R1 15 - - - - 0 0 0 0

R2 12

Harvest R1 16 1 3 8 3 3,8,3 1 6.25 18.7 50.0 18.7 2 4 5 7 √

mycelium
/sporangia R2 14 2 9 1 2 2,7,1,2 2 14.2 64.2 7.14 14.2 2 3 6 8 √
from grain
& soil R3 13 1 7 2 2 7,2,2 1 7.69 53.8 15.3 15.3 2 4 5 7 √
drench
R4 11 - 4 1 2 4,1,2 0 36.6 9.09 18.1 4 4 4 √

Control R1 12 - - - -

R2 11
Experiment No. PB- 004-10

Title: Effect of growth stage on PB development

Objective:
1. To identifies the most vulnerable stage of growth of pigeonpea against PB
development.
2. To identify the resistance stage of plant growth against PB
3. To identify the correlation between growth stage and PB symptom development.

Methods and materials:

1. Inoculation technique: Best method form inoculation techniques (Experiment:003-10)

2. Inoculum form: Mass multiplied grains or plant derby of Pdc 14

3. Host- Growth stage: 7, 15, 30, 45, 60, 75, 90

Varity- HY3C

4. Environment: Controlled environment Growth chamber- Temp- 25

Greenhouse- temp-28
5. Design of experiment
Treatments: 7+1
Replication: 3
No. of plants/ pot:5
6. Observation
6.1.1. Temp: Max & min
RH
Light
6.1.2. Progress of disease development
0 day- inoculation
1 day- incubation period (IP)
Latent period (LP)
Typical symptoms on different plant parts
Type of symptoms on foliar and root
6.1.3. Termination of the experiment
≥ 80% plant showing the typical PB symptoms
6.1.4. Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.

7. Data analysis: Collection compilation and statistical analysis with graphs,

dendograms ete.
8. Conclusion: Whether it needs repetition- Yes/ no

Time line:
Host- Growth stages : 7, 15, 30, 45, 60, 75, 90 (5th july- 28 sept.)
Growth stages: 15, 30, 45 days
Environment: GH, GC
Date of sowing: 5th july
No of pots: 9 big pots 8 1/2 inch
Age of plants to 28th sept: 90 days
Date of sowing: 19 july
Age of plants to 28th sept: 75 days
No of pots: 9 big pots 8 1/2 inch
Date of sowing: 2nd Aug
Age of plants to 28th sept: 60 days
No of pots: 9 big pots 8 1/2 inch
Growth stages: 15, 30, 45 days
Date of sowing: 16th Aug
Age of plants to 3th October: 45 days
No of pots: 6+6 (12) big pots 6”
Date of sowing: 30th Aug
Age of plants to 28th sept: 30 days
No of pots: 6+6 (12) big pots 6”
Date of sowing: 14th sept
Age of plants to 28th sept: 15 days
No of pots: 6+6 (12) big pots 6”
Date of inoculum multiplication: 14th sept
Date of sowing: 21th sept
Age of plants to 28th sept: 7 days
No of pots: 6+6 (12) big pots 6”
No. of plants: 5
Date of sowing: 14th sept
Age of plants to 28th sept: 15 days
Date of inoculation on to pots: 28th sept

Experiment No. PB- 005-10

Title: Standardization of the inoculum conc./quantity, method and form

of inoculum during inoculation methods

Objective:

1. To determine the quantity of inoculum needed for maximum infection

2. To determine the suitable form of inoculum for disease development.

Methods and materials:

1. Inoculation technique: Best method form inoculation techniques (Experiment:003-10)

2. Inoculum form: Mass multiplied grains or plant derby of Pdc 14

3. Host- Growth stage: 10 days

Variety- HY3C

4. Environment: Controlled environment- Growth chamber- Temp- 25

Greenhouse- temp-28

5. Design of experiment:
Treatments:
Replication: 3
No. of plants/ pot:5

6. Observation

6.1. Temp: Max & min

RH
Light
6.2. Progress of disease development
0 day- inoculation
1 day- incubation period (IP)
Latent period (LP)
Typical symptoms on different plant parts
Type of symptoms on foliar and root
6.3. Termination of the experiment
 ≥ 80% plant showing the typical PB symptoms
6.4. Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.

7. Data analysis: Collection compilation and statistical analysis with graphs,

dendograms ete.

8. Conclusion: Whether it needs repetition- Yes

Time line:
Gains sterilization: 6.07.10

Debris (stem) sterilization: 6.07.10

Quantity/conc.: 5, 10, 20, 30, 40g/ half kg soil
Each conc. replications: 3
Each flask 100ml: 10g/half kg soil (5’’ pot)
Total flasks: 15 flasks
Environment: 2
Total flasks: 15+15=30
Forms: pp grain, stem bits
Date of inoculation on to media: 8.7.10
Pots sowing: 15+15+6=36 for grains
Total pots: 36+36= 72 pots
Seeds: 6-7seeds/pot
Date of sowing: 06.7.10
Date of inoculation: 16.7.10
Date of observations started: 17.7.10

Experiment No. PB- 006-10

Title: Variability study of Pdc isolate collected from different locations

Objective:
1. To study the diversity of Pdc isolate collected from different geographical locations
2. To established the taxonomic group of the Pdc in India

6.1. Morphological variability

6.1.1. Cultural and morphological character of the different isolate of Pdc

6.1.2. Study the different methods for early and more sporangial production and zoospore
release

6.1.3. Study the conditions favorable for oospores formation by different methods

6.1.4. Study the effect of different media on Pdc isolate based on cultural and
morphological characters.

Time line:
Morphological studies:
No. of isolates: 27
V8 juice agar preparation: 23th july

Date of inoculation into petriplates: 26th july

No. of plates required: 27 X 3= 81
th
Date of observations on plates: 27 july

Please add methods and materials after each experiment

6.2. Pathogenic variability

6.2.1. Study the aggressiveness of Pdc and Fu with 1. Pdc alone 2. Fu alone 3. Pdc +
Fu

6.2.2. Study the predisposing factors for pathogenecity of PB

Please add methods and materials after each experiment

6.3. Molecular characterization

6.3.1. Characterization of Pdc pure culture at molecular level

Methods and materials:

Isolate: ICRISAT isolate of Pdc
Design of experiment: 1. Isolation of DNA from Pdc isolate by gene specific primers
2. Cloning of amplified product in suitable vector system.
3. Sequencing of cloned fragment either in house or by commercial service

Data analysis: Sequence analysis for making dendogram and sequence identity
Conclusion: Whether it needs repetition- Yes/no

6.3.2. Variability study of Pdc pure culture at molecular level collected from different
geographical locations

Isolate: ICRISAT isolate of Pdc

Design of experiment: 1. Isolation of DNA from different Pdc isolates by gene specific
primers
2. Cloning of amplified products in suitable vector system.
3. Sequencing of cloned fragment either in house or by commercial service

Data analysis: Sequence analysis for making dendogram and sequence identity
Conclusion: Whether it needs repetition- Yes/no

Experiment No. PB- 007-10

Title: Effect of inoculum depth on pigeonpea

Objective: To study the relationship between inoculum depth and PB disease

Methods and materials:

Inoculum form: Mass multiplied grains of Pdc 14

Host- Growth stage: Sowing on inoculated soil

Varity- HY3C

Environment- Controlled environment Growth chamber- Temp- 25

Greenhouse- temp-28
Design of experiment
Treatments: - 1. Infected grains on the bottom of the pot 2. Infected grains on the middle of
the pot 3. Infected grains on the 1 cm below the surface.
Replication: 3
No. of plants/ pot:5
Observation
Temp: Max & min
RH
Light
Progress of disease development
After germination
1 day after germination- incubation period (IP)
Latent period (LP)
Typical symptoms on different plant parts
Type of symptoms on foliar and root
Termination of the experiment
≥ 80% plant showing the typical PB symptoms
Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.
Data analysis: Collection compilation and statistical analysis with graphs, dendograms ete.
Conclusion: Whether it needs repetition- Yes/no

Tima line: Date of inoculation: 14.07.10

Date of observation: 15.07.10 onwards

Experiment No. PB- 008-10

Title: Effect of Pdc and Fu both on pigeonpea to know the aggressiveness of

Pdc and Fu in greenhouse
Objective: To investigate, which isolate (Pdc and Fh) is more dominating to other

Methods and materials:

Inoculum form: Pure culture of Pdc and Fu

Host- Growth stage: 10 days old seedling

Varity- HY3C

Environment- Controlled environment Growth chamber- Temp- 25

Greenhouse- temp-28
Design of experiment
Treatments: 1. Pdc alone 2. Fu alone 3. Pdc and Fu both
Replication: 3
No. of plants/ pot:5
Observation
Temp: Max & min
RH
Light
Progress of disease development
0 day after inoculation
1 day after inoculation- incubation period (IP)
Latent period (LP)
Typical symptoms on different plant parts
Type of symptoms on foliar and root
Which is more aggressive Pdc or Fu
Termination of the experiment
≥ 80% plant showing the typical PB symptoms
Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.
Data analysis: Collection compilation and statistical analysis with graphs, dendograms ete.
Conclusion: Whether it needs repetition- Yes/no

Experiment No. PB- 009-10

Host range

We should discuss with Sir

Experiment No. PB- 010-10

Title: Effect of environment/ physical factors on pathogenecity of PB

Objective: To study the effect of different factors on pathogenesity of PB

Methods and materials:

Inoculum form: Mass multiplied grains/debris of Pdc 14

Host- Growth stage: 10 days old seedlings

Varity- HY3C

Environment- Controlled environment Growth chamber

Design of experiment
Treatments: 1. Temp 2. Light 3. RH 4. Moisture
Replication: 3
No. of plants/ pot:5
Observation
Temp: Max & min
RH
Light
Progress of disease development
After inoculation
1 day after inoculation- incubation period (IP)
Latent period (LP)
Typical symptoms on different plant parts
Type of symptoms on foliar and root
Termination of the experiment
≥ 80% plant showing the typical PB symptoms
Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.
Data analysis: Collection compilation and statistical analysis with graphs, dendograms ete.
Conclusion: Whether it needs repetition- Yes/no

Experiment No. PB- 011-10

Title: Screening of pigeonpea for host plant resistant

Objective: To find out superior germplasm/ variety resistant or tolerant against PB

Methods and materials:

1. Inoculation technique: Soil mixing
2. Inoculum form: Mass multiplied on grains or debris of Pdc 14
3. Host- Growth stage: 10 days old plants grown on sterile red soil
Varity/germplasm: As much as available form the prvious study
as well as new
4. Environment: Controlled environment Growth chamber- Temp- 25
Greenhouse- temp-28
5. Design of experiment
Treatments: 1+1
Replication: 3
No. of plants/ pot:5
6. Observation
6.1. Temp: Max & min
RH
Light
6.1.2. Progress of disease development
0 day- inoculation
1 day- incubation
Typical symptoms development
6.1.3. Termination of the experiment
≥ 80% plant showing the typical PB symptoms
6.1.4. Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.

7. Data analysis: Collection compilation and statistical analysis with graphs, dendograms
ete.

8. Conclusion: Whether it needs repetition- Yes/ No

Experiment No. PB- 012-10

Title: Role of biochemical and histo-pathological component for HPR of

pigeonpea

Objective: To study the exact biochemical mechanism for HPR in pigeonpea

12.1. Role of biochemical components for HPR

Methods and materials:

1. Inoculation technique: Best method form inoculation techniques (Experiment:003-10)

2. Inoculum form: Mass multiplied grains or plant derby of Pdc 14

3. Host- Growth stage: 10 days

Variety- HY3C (Control) and one resistant variety

4. Environment: Controlled environment- Growth chamber- Temp- 25

5. Design of experiment:
Treatments:
Replication: 3
No. of plants/ pot: 5
2 hrs after inoculation, sample taken for biochemical assays form inoculated and control.
Then after every 2 hrs gap the assay continued.

6. Observation

6.1. Temp: Max & min

RH
Light
6.2. Change of biochemical components
0 day- inoculation
2 hrs, 4, 6, 8…..
Typical biochemical changes
6.3. Termination of the experiment
When data not varying
6.4. Precautions: Same environment should be maintained through out the experiment.

7. Data analysis: Collection compilation and statistical analysis with graphs ete.

8. Conclusion: Whether it needs repetition- Yes/no

12.2. Changes of biochemical components after inoculation of Pdc in pigeonpea

Methods and materials:

1. Inoculation technique: Best method form inoculation techniques (Experiment:003-10)

2. Inoculum form: Mass multiplied grains or plant derby of Pdc 14

3. Host- Growth stage: 10 days

Variety- HY3C

4. Environment: Controlled environment- Growth chamber- Temp- 25

5. Design of experiment:
Treatments:
Replication: 3
No. of plants/ pot: 5
2 hrs after inoculation, sample taken for biochemical assays form inoculated and control.
Then after every 2 hrs gap the assay continued.

6. Observation

6.1. Temp: Max & min

RH
Light
6.2. Change of biochemical components
0 day- inoculation
2 hrs, 4, 6, 8…..
Typical biochemical changes
6.3. Termination of the experiment
When data not varying
6.4. Precautions: Same environment should be maintained through out the experiment.

7. Data analysis: Collection compilation and statistical analysis with graphs ete.

8. Conclusion: Whether it needs repetition- Yes/no

Experiment No. PB- 013-10

Title: Effect of Pdc on pigeonpea at field

Objective: 1. To implement standardized greenhouse inoculation technique (s) in field

2. To study the incidence and severity in field level

13.1. Field inoculation: Implementation of greenhouse techniques in field

Methods and materials:

1. Inoculation technique: Best method form inoculation techniques (Experiment:003-10)

2. Inoculum form: Mass multiplied grains or plant derby of Pdc 14

3. Host- Growth stage: 30 days

Variety- HY3C

4. Environment: Field

5. Design of experiment:
Treatments: Soil mixing
Plot: 9x9

Observation
Temp: Max & min
RH
Light
Progress of disease development
After inoculation
1 day after inoculation- incubation period (IP)
Latent period (LP)
Typical symptoms on different plant parts
Type of symptoms on foliar and root
Termination of the experiment
≥ 80% plant showing the typical PB symptoms
Precautions: Flooding with sterilized water
Plating of water contaminants
Exposing plats in experiment environments to catch contaminants.
Data analysis: Collection compilation and statistical analysis with graphs, dendograms ete.
Conclusion: Whether it needs repetition- Yes/no

13.2. Succession of fungi (off session/ crop session)

????

13.3. Survey of different pigeonpea fields of ICRISAT and Andhra

Pradesh

Objective: 1. To study the disease incidence severity of PB of pigeonpea

2. To isolate sample form different locations.
Experiment No. PB- 014-10

Title: Mode of inheritance and allelic relationship of genes for resistance to

PB

We should discuss with Sir