Sunteți pe pagina 1din 7

Biosensors and Bioelectronics 17 (2002) 835 /841

‘Missing mass’ effect in biosensor’s QCM applications

M.V. Voinova *, M. Jonson, B. Kasemo
Department of Applied Physics, Chalmers University of Technology and Göteborg University, S-412 96 Göteborg, Sweden

Received 25 May 2001; received in revised form 11 January 2002; accepted 21 January 2002


Nowadays, liquid applications of quartz crystal microbalance (QCM) opened a way for in situ studies of proteins, vesicles and
cells adsorbed from the solution onto the QCM surface. The sensitivity of QCM to the viscoelasticity of the adsorbed biomaterial
can be a reason of the experimentally observed deviation from a linear dependence of QCM resonant frequency on mass deposition
(the so-called Sauerbrey relation) and can limit its application for biosensoring. Presented here rigorous theoretical analysis explains
the deviation from ideal mass response of soft overlayers in the contact with liquid. The fundamental result of the theory is the
analog of Sauerbrey relation for layered viscous/viscoelastic medium which can be exploited for the correct physical interpretation
of QCM experimental data in biofluids, in particular for measurements of the ‘true’ surface mass of adsorbed biomolecular films.
We predict a new physical effect ‘missing mass’ of the sample in liquid phase measurements and compare the results given by our
theory with QCM measurements on supported membranes. # 2002 Elsevier Science B.V. All rights reserved.

Keywords: Acoustical biosensors; Quartz crystal microbalance; Supported membranes; Viscoelasticity

1. Introduction Fredriksson et al., 1998; Höök et al., 1999; Keller and

Kasemo, 1998).
Successful characterization of biomolecular systems The experimental fact that the quartz crystal resona-
in their natural aqueous environment is the important tors are sensitive to viscoelastic properties of tested
objective of biosensoring. The quartz crystal microba- material (Voinova et al., 1997, 1999a,b Reed et al., 1990;
lance (QCM) which can operate in fluids, offers such Rodahl et al., 1997; Bandley et al., 1997 Lucklum and
straightforward possibility (Rodahl and Kasemo, 1996). Hauptmann, 1997; Johannsmann et al., 1992; Nwankwo
Polymer coated or biochemically modified quartz sur- and Durning, 1998), limits their application as a
face forms a ‘tolerant’ biological interface which is microbalance, i.e. for the precisious mass detection of
available for further mounting of biomolecular com- biological materials in liquid phase measurements. It
plexes exposed to water solution. Recently, the applica- seems crucial that, an adequate physical model is chosen
tion of QCM as a sensor element for the electronic nose for a quantitative interpretation of experimentally ob-
(DiNatale et al., 2000) and affinity immunosensor has served deviation from ideal Sauerbrey behavior in
been reported elsewhere (Sakti et al., 1999, 2000). biosensor’s QCM measurements. A vast literature on
Biosensor systems based on a QCM have become a QCM experiments and theory (see, for review, Hengerer
suitable tool for in situ measurements in biofluids, in et al., 1999; Rodahl and Kasemo, 1996; Voinova et al.,
particular, for on-line detection of immunological reac- 1999a,b) reveals a sharp interest to this topic. However,
tions (Hengerer et al., 1999; Sakti et al., 2000; Kößlinger the analog of Sauerbrey’s relation for systems with
et al., 1995). for surface bioelectrochemistry of redox complex rheology as to our knowledge has not been
enzymes (Buttry, 1991). adsorption of proteins, and reported yet.
adhesion of vesicles and living cell (Rodahl et al., 1997; In this communication, we present rigorous formulae
for QCM response in liquids for soft deposits when the
Sauerbrey’s relation is not valid. The main result in the
* Corresponding author. Tel.: /46-31-772-3115; fax: /46-31-41-
6884 present work */the effect of ‘missing mass’, predicts the
E-mail address: (M.V. Voinova). reduction in liquid phase measurements of surface mass
0956-5663/02/$ - see front matter # 2002 Elsevier Science B.V. All rights reserved.
PII: S 0 9 5 6 - 5 6 6 3 ( 0 2 ) 0 0 0 5 0 - 7
836 M.V. Voinova et al. / Biosensors and Bioelectronics 17 (2002) 835 /841

Ms comparing with the ‘true’ mass M of the film: When a rigid film is replaced by Newtonian bulk liquid
  of shear viscosity h , the QCM resonant frequency shift
hrv Gƒ
Ms  M 1 2 2 ; M r1 h1 ; (1) is given by Kanazawa and Cordon’s expression (Kana-
r1 G?2  Gƒ2 zawa and Gordon, 1985):
In the section, ‘Comparison with experiments and sffiffiffiffiffiffiffiffiffiffi
discussion’ we verify our theoretical results on QCM 1 rhv
Df : ; (3)
measurements on supported membranes (Fig. 1) pub- 2pmq 2
lished elsewhere (Rodahl et al., 1997; Johannsmann et
al., 1992; Höök et al., 1999; Keller and Kasemo, 1998). so the total response Df for the system of QCM rigid
film-immersed-in-water is a sum of film surface mass
(Eq. (2)) and viscous contribution of water (Eq. (3)).
However, our theoretical analysis ((Voinova et al., 1997,
2. Theoretical considerations of the QCM surface mass 1999a,b) shows that for non-rigid deposits that is not
measurements. Resonant frequency and damping of QCM valid. We found that the viscous loss of energy in the
in complex fluids overlayers causes the deviation from Sauerbrey behavior
and results in non-trivial reduction in measured surface
Let us consider a QCM oscillating in a thickness- mass of the film, Ms /M (1/a ), where M is the ‘true’
shear mode and on one side covered by a soft adsorbed film mass and the viscous correction factor a depends
layer (the geometry is depicted schematically in Fig. 1). both on mechanical properties both of overlayer mate-
We describe the motion of the QCM as the forced rial and of aqueous solution h , r . This reduction in Df
vibration of a damped oscillator driven by periodic force has been predicted by us for the first, time for the special
(Rodahl and Kasemo, 1996; Voinova et al., 1999a,b). case of pure viscous layers (Voinova et al., 1997).
When mass is deposited onto the crystal surface, the Now consider a soft (viscoelastic) material which
resonance frequency changes, For a small amount of contacts the solution and effects the Df of the quartz
mass deposited onto the QCM surface, the shift of oscillator. The material is assumed to exhibit linear
resonance frequency Df is proportional to the surface viscoelasticity if both deformations and deformation
mass M of the deposit (Sauerbrey, 1959): rates are small (Panizza et al., 1996; Lekkerkerker,
M 1997). which holds in QCM dynamic measurements.
Df˜ f0 : (2) In the linear viscoelastic regime, there is proportionality
between stored energy and strain and between strain
This equation known as Sauerbrey’s decrease of the rate and dissipated energy (Doi and Edwards, 1989).
resonant frequency (Sauerbrey, 1959), can only be used When the oscillatory shear stress of constant frequency
for thin films rigidly attached to the QCM surface. v is applied to the viscoelastic material, the general

Fig. 1. Sketch of different types of supported membranes. We also display the geometry of shear wave propagation in the system: k is the wave
vector, u denotes the in-plane displacement vector. (a) Flat lipid bilayer; (b) Langmuir /Blodgett film composed from rod-like organic molecules.
Adlayer formed by dense packed (c) small unilamellar lipid vesicles and (d) multilamellar liposomes (‘onion phase’, top view).
M.V. Voinova et al. / Biosensors and Bioelectronics 17 (2002) 835 /841 837

strain/stress relation can be written as following: When the viscosity of the top bulk liquid vanishes,
sik 2Guik : (4) viscoelastic corrections to the equivalent surface mass
appear only in higher order corrections in the film
Here sik , is the shear stress tensor, uik is the strain thickness:
tensor, G *(v )/G ?(v )/iGƒ(v ) is the complex fre-  
hrv h2 rv2 G?
quency-dependent shear modulus of viscoelastic fluid; Df : 1 (6)
the real part G ?(v ) is a storage modulus while the 2pmq 3 G?2  Gƒ2
imaginary one G ƒ(v ) is a loss modulus, v is a circular Let us discuss now the physical origin of damping in
frequency. The most well known mathematical formula- biofluid’s QCM measurements. While a rigid film
tions of linear viscoelasticity are the Maxwell model of attached to the quartz surface shows no dissipation at
relaxation in highly viscous fluids and the Kelvin/Voight no-slip conditions, a damping due to a bulk viscous
model of viscoelastic solids. Below we consider an liquid (as was originally reported by Stockbridge, 1966
arbitrary viscoelastic material characterized by a shear and later revealed by Rodahl and Kasemo, 1996)
complex modulus G *(v ) and discuss the results for the depends on its density r and shear viscosity h :
special cases of supported membranes in the next sffiffiffiffiffiffiffiffi
Section. 1 2rh
The shift of resonant frequency Df and dissipation DD : :
mq v
factor DD , due to the soft deposit on the quartz surface,
can be calculated as a real and imaginary parts of the In case of a layered viscous (viscoelastic) medium, DD
frequency shift of viscoelastically loaded quartz oscilla- is a complex functions of thickness, mass and viscoelas-
tor with no-slip boundary conditions (since in present tic properties of the material. We found that the
communication, we use the model developed by us dissipation factor of the quartz oscillator covered with
earlier, we will not repeat here the details of calculations soft overlayer in liquid phase measurements is:
and refer our readers to Voinova et al., 1997). Within   2 
1 h2 h2 h1 vG?
this model, using the balance between dissipated and DD̃ : 2 (7)
stored energy in the system of the soft film exposure to pfmq d2 d2 G?2  Gƒ2
one side of the crystal and to another one of the From (Eq. (7)). viscous corrections due to the
solution, we found the following expression for Df: dissipation in liquid phase /h2d2 appear already in
h2 hrv the first order in the film thickness, while in a gas phase
Dftotal :  1 1 or vacuum the same thin overlayer will dissipate much
2pmq d2 2pmq
smaller energy /h3:
2 h2 2 Gƒ
 1 ; (5) h3 r2 v3 Gƒ
r1 d2 G?2  Gƒ2 DD̃ : (8)
3pfmq G?2  Gƒ2
where the index ‘1’ corresponds to the thin soft overlayer
Comparing formulae (Eqs. (5) and (7)) and (Eqs. (6)
and index ‘2’ denotes liquid bulk phase, respectively,
and (8)), we conclude that viscoelastic effects become
d2 //2h2/r2v is a viscous length of bulk newtonian
more pronounced when the system operates in a bulk
liquid (Landau and Lifshitz, 1997). Note that in (Eq.
liquid phase and thus one can determine storage G? and
(5)) the viscous term appears in first order correction in
loss G ƒ moduli of soft deposits with higher accuracy
the film thickness h . Then the reduction (Eq. (1)) in
even for ultrathin films.
measured surface mass Ms in comparison with the ‘true’
mass M of the film, is due to the viscous loss of energy
of the quartz oscillator both in the soft overlays (/G ƒ)
and in the liquid bulk phase (h2r2). A simple analysis of
the expression (Eq. (5)) shows, that the resonance 3. Comparison with experiments and discussion
frequency has a minimum when the viscoelastic ratio
X /G ?/G ƒ is equal to unity. (It should be pointed out, In this section, we verify our theoretical results (a
since in biofluid’s QCM measurements the thickness h ‘missing mass’ effect) by comparing the QCM response
of adlayer and its viscoelasticity G* can both vary predicted in the theory with experimental data on
during the adsorption process, one has to monitor. e.g. Langmuir /Blodgett /Kuhn films and supported mem-
h independently by other method while G ? and G ƒ can branes published elsewhere (Voinova et al., 1999a,b;
be derived from Df and DD measured simultaneously). Rodahl et al., 1997; Keller and Kasemo, 1998). In
In Figs. 2 and 3, we display the calculated resonant contrast with Langmuir /Blodgett films, water environ-
frequency and dissipation factor versus film thickness ment is essential for lipid bilayer membranes since it
and loss moduli for viscoelastic film in liquid environ- maintains the structure and fluidity of lipid membrane
ment. via a balance of hydrophobic and hydrophilic forces.
838 M.V. Voinova et al. / Biosensors and Bioelectronics 17 (2002) 835 /841

Fig. 2. Calculated resonance frequency shift df of 3 MHz quartz crystal versus overlayer thickness and loss modulus G ?? at given elastic modulus
G ?/106 dyn cm 2; system operates in a bulk water solution (bulk water contribution was subtracted).

Supported membranes is a convenient model system hm /0.23 /106 cm is a monolayer thickness and
whose physical properties are similar to those of lipid Dfm /13 Hz is the frequency shift monitored for the
bilayer of the biological membranes (Sackmann, 1996). monolayer (Keller and Kasemo, 1998). Thus, the
Membranes-planar (Fig. 1a) or of closed type (vesicles; reduction in frequency response is df /51 Hz. This
Fig. 1c) form a soft biological interface, with peculiar deviation from Sauerbrey behavior and a large dissipa-
viscoelastic properties. In the supported membranes tion DD can be explained by ‘missing mass’ effect
technology, viscosity and elasticity of lipid bilayer play predicted in our theory. By setting for adlayer of
a key role in membrane assembling on surfaces (Sack- vesicles X /G? /G ƒ :/0.1 and G? :/105 dyne cm 2, we
mann, 1996). Strongly adhesive surfaces can give rise to find from (Eq. (5)) that df :/50 Hz and from (Eq. (7))
membrane failure and subsequent fusion of vesicles or to that. dD /3:/106 which is consistent with experi-
appearance of single bilayers with free edges. Alterna- mental data.
tively, adsorption of vesicles on moderately adhesive Since at the same time the lipid bilayer of thickness
substrates can result in morphological transformations 2hm demonstrated a pure viscous response (DD /0/
or formation of an intact vesicles monolayer (Höök et Dfm /2Dfm //26 Hz (Keller and Kasemo, 1998)), we
al., 1999; Keller and Kasemo, 1998; Sackmann, 1996). conclude that flat bilayers and intact dense packed
It was found by Keller and Kasemo, 1998, that the vesicles differ in mechanical properties: flat bilayer
adsorption of lipid vesicles (egg phosphatidylcholine behaves as 2D pure viscous fluid with negligible
(PC) small unilamellar vesicles of radius R /12.5 nm) resistance to shear in-plane stress (Helfrich, 1973) while
onto an oxidized gold surface is accompanied by a large adlayer built up from vesicles demonstrates a pro-
resonant frequency shift (Df//90 Hz) and a large nounced viscoelasticity. Apparent viscoelasticity of the
dissipation (DD /3 /106). Now let us estimate the latter can lie attributed to the assumption that dense
‘ideal’ pure mass response of this system (assuming that packed vesicles onto the oxidized gold forms a viscoe-
a bulk water contribution (Eq. (3)) has already been lastic ‘supramolecular fluid’ where individual vesicles
subtracted front Df). Sauerbrey’s formula (Eq. (2)) gives play role of the ‘supramolecules’ (Voinova, unpub-
Df /Dfmh1/hm //141 Hz where h1 / 2, R /2.5 /10 6 lished). (The formation of strong, weak and metastable
cm is the thickness of the adlayer r1 :/1 g cm 3 (PC liquids from colloidal ‘supramolecules’ has recently been
bilayer density at room temperatures (Petrov, 1999)), discussed in detail by Lekkerkerker (1997).)
M.V. Voinova et al. / Biosensors and Bioelectronics 17 (2002) 835 /841 839

Fig. 3. Calculated damping of 3 MHz quartz crystal versus overlayer thickness and loss modulus G ?? for the same conditions as in Fig. 2.

Another example of supported membranes-the so- who calculated and measured the frequencies of QCM
called onion phase, is shown schematically on Fig. 1d. resonances and the corresponding damping constants of
Panizza et al. (1996) provided a clear evidence that dense viscoelastic Langmuir /Blodgett /Kuhn films (sketched
packed multi-lamellar liposomes (onion phase) exhibit in Fig. 1b). The authors (Johannsmann et al., 1992)
viscoelastic properties. They have measured the elastic suggested to measure the equivalent mass on various
(storage) shear modulus G ?(v ) and a loss modulus G ƒ harmonics and then plot this characteristic versus the
(v ) of the onion phase by means of a low frequency square of the frequency to estimate the viscoelastic
rheometry. It should be mentioned that the QCM compliance J(v )/1/G * of the film and to study
measurements of shear elastic moduli of biomaterials whether viscous and elastic compliance arc frequency-
can provide essential information about their dynamical dependent. As an extension of their idea, we noticed that
behavior at high frequencies. in gas phase measurements, the frequency behavior of
It is useful to compare a response of QCM to the QCM characteristics in the linear viscoelasticity
viscoelastic fluid and viscoelastic solid films in gas phase
region, viz. Df(v ), can also test for the type of material-
(or vacuum). For the special case of the viscoelastic
either viscoelastic fluid or viscoelastic solid. From our
(Maxwell) fluid, we found that the resonant frequency
results (Eq. (9) and Eq. (10)) for DfM and Dfv , it follows
shift is a function on the film shear elasticity GM :
that we expect a straight line if Df(v ) is plotted versus
hrv h2 rv2 v2 for the case of a Maxwell fluid and a deviation from
DfM : 1 (9)
2pmq 3GM a straight line for other types of viscoelastic materials. In
the case of Maxwell fluid, the slope of the straight line
while the viscoelastic solid (Voight/Kelvin) film is a gives the equilibrium shear elastic modulus GM of the
function of both viscous h and elastic G moduli [9]: material (the viscosity h of the Maxwell film can then be
  deduced directly from the measured values of DD if the
hrv h2 Gv2 r
Dfv : 1 : (10) film thickness and density are known).
2pmq 3(G2  h2 v2 )
The usage of QCM for biological arid biomedical
The results of our model for gas phase are in purposes brings out the problem of accounting for the
agreement with those of Johannsmann et al. (1992) complex rheology of the biological interface, which is
840 M.V. Voinova et al. / Biosensors and Bioelectronics 17 (2002) 835 /841

dominated by viscous effects. In some special cases or road to biosensoring, is QCM-immunosensor and QCM
for particular type of shear deformations, the linear as mechanical spectroscopy device assembled in arrays.
viscoelasticity of polymers can be treated within either
the Maxwell or the Voight/Kelvin schemes. The results
of the Yoight/Kelvin model can readily be applied to 4. List of symbols
quartz crystal acoustic measurements of adsorbed
proteins, which conserve their shape during adsorption D dissipation factor
and do not flow under shear deformation as well as to f0 fundamental resonant frequency of QCM
polymer films far from the glass transition region. The G* shear complex modulus
Maxwell fluid scheme is appropriate for polymer solu- G? storage modulus
tions that are pure liquids (at low shear rates) and Gƒ loss modulus
polymers in the amorphous state and in the vicinity of J* viscoelastic compliance
the polymer liquid-glass transition (Sperling, 1986). h overlayer thickness
Viscoelastic materials with more complex rheology mq (surface) mass of the quartz plate
(e.g. cells, membranes, liquid crystal polymers etc.) can M (surface) mass of the deposit
be described by a combination of these two basic R radius of lipid vesicles
viscoelastic schemes. They can be analyzed in a similar nik strain tensor
as we have done here way by substitution of the
corresponding expressions for the complex shear mod- Greek letters
ulus G * in the general results (Eqs. (5) /(8)). For a viscous correction factor
instance, the complex shear modulus: d viscous length
h shear viscosity coefficient
G1  ivh1 1
G1   : (11) r density
ivh1 G1 G2  ivh2 sik stress tensor
t relaxation time
well describes, a viscoelasticity of the lyotropic multi- v circular frequency
lamellar vesicles. [26] (‘onion phase’) sketched in Fig. 1d.
It is convenient to describe the frequency behavior of Indices
a complex viscous medium exhibiting a spectrum of / corresponding to the non-slip conditions
relaxation times in terms of the three-parameter model M monolayer
h* /h /(1/ivt)b of the complex viscosity suggested by M maxwell viscoelastic fluid
Reed et al. (1990). In this model, h is the viscosity of the V Voight/Kelvin viscoelastic solid
liquid; b is a characteristic exponent related to the s surface
distribution of relaxation times t. In the same manner,
we can introduce in our model a complex shear modulus
of the form:

G Acknowledgements
(1  i=wt)b
One of us (M.V. Voinova) is indebted to P. Haupt-
mann for his discussion of QCM immnnosensor’s
which is a generalization for the case of a relaxation time
applications, to E. Terentjev for his fruitful comments
distribution characterized by the exponent b . In parti-
to our model of supramolecular viscoelastic fluid and
cular, the Maxwell fluid corresponds to the value b/1.
C.-Y.D. Lu for the important correspondence and
Besides the experimental concern on viscoelastic
demonstration of their rheometric frequency-dependent
corrections of surface mass measurements, an important
measurements on onion multilamellar liposomes. This
problem for the application of the QCM technique to
work was supported financially by the Royal Swedish
biosensoring remains. How can the device recognize
Academy of Sciences (KVA) and the Swedish TFR.
what type of material is being probed except by
detection of an ‘anonymous’ deposited mass? We
suggest here a mechanical test of how to distinguish
Maxwell viscoelastic materials among others by measur- References
ing the QCM response on different frequencies. Another
Bandley, H.L., Hillman, A.R., Brown, M.J., Martin, S.J., 1997.
way is a direct biochemical modification of quartz
Viscoelastic characterization of etectroactive polymer films at the
surface for selective binding of tested biological mole- electrode/solution interface. Faraday Discuss. 107, 214 /228.
cules (Hengerer et al., 1999; Sakti et al., 1999; Sakti et al. Buttry, D.A., 1991. The quartz crystal microbalance as an situ tool in
2000; Kößlinger et al., 1995). Perhaps the successful electrochemistry. In: Abruña, II.D. (ed.), Electrochemical inter-
M.V. Voinova et al. / Biosensors and Bioelectronics 17 (2002) 835 /841 841

faces: modern technique for in-situ interface characterization, Panizza, P., Roux, D., Vuillaume, V., Lu, C.-Y., Cates, M.F., 1996.
VCII. Viscoelasticity of the onion phase. Langmuir 12, 248 /252.
Doi, M., Edwards, S.F., 1989. The Theory of Polymer Dynamics. Petrov, A.G., 1999. The Lyotropic State of Matter. Gordon and
Clarendon Press, Oxford. Breach publication.
Fredriksson, C., Kihlman, S., Rodahl, M., Kasemo, B., 1998. The Reed, C.E., Kanazawa, K.K., Kaufman, J.H., 1990. Physical descrip-
piezoelectric quartz crystal mass and dissipation sensor: a means of tion of a viscoelastically loaded AT-cut quartz resonators. J. Appl.
studying cell adhesion. Langmuir 14, 248 /251. Phys. 68, 1993.
Helfrich, W., 1973. Elastic properties of lipid bilayers: theory and Rodahl, M., Höök, F., Fredriksson, C., Keller, C.A., Krozer, A.,
possible experiments. Z. Naturforsch. 28C, 693 /703. Brzezinski, P., Voinova, M., Kasemo, B., 1997. Simultaneous
Hengerer, A., Decker, J., Prohaska, E., Hauck, S., Kößlinger, C., frequency arid dissipation factor QCM measurements of biomole-
Wolf, H., 1999. Quartz crystal microbalance (QCM) as a device for cular adsorption and cell adhesion. Faraday Discuss. 107, 229 /
the screening of phage libraries. Biosens. Bioelectron. 14, 139 /144. 246.
Höök, F., Rodahl, M., Keller, C., Glasmästar, K., Fredriksson, C., Rodahl, M., Kasemo, B., 1996. On the measurement of thin liquid
Dahlqvist, P., Kasemo, B., 1999. The dissipative QCM-D techni- overlayers with the quartz crystal microbalance. Sens. Actuators A.
que: interfacial phenomena, and sensor applications for proteins, 54, 448 /462.
membranes, living cells and polymers. 1999 Joint Meeting EFTF- Sackmann, E., 1996. Supported membranes: scientific and practical
IEEE IFCS, 966 /972. applications. Science 271, 43 /48.
Johannsmann, D., Mathauer, K., Wegner, C., Knoll, W., 1992. Sakti, S.P., Röstler, S., Lucklum, R., Hauptmann, P., Bühling, F.,
Viscoelastic properties of thin films probed with a quartz-crystal
Ansorge, S., 1999. Thick polystyrene-coated quartz crystal micro-
resonator. Phys. Rev. B46, 7808.
balance as a basis of a cost effective immunosensor. Sens.
Kanazawa, K.K., Gordon, J.G., 1985. The oscillation frequency of a
Actuators 76, 98 /102.
quartz resonator in contact with a liquid. Anal. Chim. Acta 175,
Sakti, S.P., Hauptmann, P., Zimmermann, B., Bühling, F., Ansorge,
99 /105.
S., 2000. Disposable QCM-immunosensor for practical qualitative
Keller, C., Kasemo, B., 1998. Surface specific kinetics of lipid vesicle
and quantitative diagnosis. Eurosensors XIV. Gopenhagen, 475 /
adsorption measured with a quartz crystal microbalance. Biophys.
J. 75, 1397 /1402.
Sauerbrey, G., 1959. Verwendung von Schwingquarzen zur
Kößlinger, C., Uttenthaler, F., Drost, S., Alberl, F., Wolf, H., Brink,
G., Stanglmair, A., Sackmann, E., 1995. Comparison of the QCM Wägung Dünner Schichten und zur Mikrowägung. Z. Physik
and the SPR method for surface studies and immunological 155, 206 /222.
applications. Sens. Actuators B. 24 /25, 107 /112. Sperling, L.H., 1986. Introduction to Physical Polymer Science. Wiley.
Landau, L.D., Lifshitz, E.M. 1997. Course of Theoretical Physics, Stockbridge, C.D., 1966. Effects of gas pressure on quartz-crystal
three ed. vol. 6. Fluid mechanics. microbalances. In: Vacuum Microbalance Techniques, vol. 5.
Lekkerkerker, H.N.W., 1997. Strong, weak and metastable liquids. Plenum Press, pp. 147 /148.
Physica A. 244, 227 /237. Voinova, M.V., Jonson, M., Kasemo, B., 1997. Dynamics of viscous
Lucklum, R., Hauptmann, P., 1997. Determination of polymer shear amphiphilic films supported by elastic solid sub-strates. J. Phys.
modulus with quartz crystal resonators. Faraday Discuss. 107, Condens Matter 9, 7799 /7808.
112 /202. Voinova, M.V., Rodahl, M., Jonson, M., Kasemo, B., 1999a.
DiNatale, C., Paolesse, R., Macagnano, A., Mantini, A., D’Amico, A., Viscoelastic acoustic response of layered polymer films at fluid-
Legin, A., Lvova, L., Rudnitskaya, A., Vlasov, Y., 2000. Electronic solid interfaces: continuum mechanics approach. Physica Scripta
nose and electronic tonque integration for improved classification 59, 391 /396.
of clinical and food samples. Sens. Actuators B. 64 (1 /3), 15 /22. Voinova, M.V., Rodahl, M., Jonson, M., Kasemo, B., 1999b.
Nwankwo, E., Durning, C.J., 1998. Mechanical response of thickness- Viscoelastic acoustic response of layered polymer films at fluid-
shear mode quartz-crystal resonators to linear viscoelastic fluids. solid interfaces: continuum mechanics approach. Physica Scripta
Sens. Actuators A. 64, 119 /124. 59, 391 /396.