eeoruary 1980 indo ts on or sable) EICIS ig oo IFES en dea io $ HRC bas » $ sxe uasoen 12039 seta A te _ Pasian ag 3 sa sion 1300 2 SRC eee as cue an 3 tke ; ies a i sre Geeta nossa ‘though the number of PFC per spleen was consi (Gor example, LTC 25, Table : Table 2). "At present, we Know litle about the long-term fote ofthese injected helper T cells in vivo C6 al ice ho 1h Hacks Nn rn or bRe Foe bly higher Boosting of mice which had been se rtituted with the SRC-specific clone 1/25 and primed 21 dlays earlier, gave rise to even bighe ernumbersof PFC against the omsigoos ate eee Ths suggest hat these her Te ilps ae eat este Teel growth factor”. an cation" the reconstitu tate, Inthe experiments presented here, westu “asote requirement fo fing ells may revert there to a resting ily, and have not observed. ved a significant switch 0 Oy By using various clones and immunisation schedules, We whether the same helper T cell can ould be able to decide taxi help for various a tibody classes. Sar results show that helper T cells reveal the Fa “per in wt, these helper T eels are cexifity in vitro and in 2 resticte out Reconstitution of T-cell-deficient mice come Research Unit, Chr ieled and passagedin cl ‘tamin By, synthesis by fnaa small intestinal, bacteria SAthert, V. Te Mathan & S.J. Baker ore oon Tam Sa Inia sn phystological amounts of vitamin shed by the ntsinsl factor SE Riy in he ileum ies of sitarain Bra Sea bacteria in the colon ded afew clones IgG produc- ime degree of The restriction mapping to the Ft of the IB with T cells Iture provides anew tool for studying fn Medic! College Hosni feyanocobatarnin) “tated. mechanism in ar facees contain appreciable ‘or starin Bu lie anat duce age ioavideal, Hoeven, aivigon psierabie meroior2 and ne also often hart ae ae ore_etesive im aenty an subjects’. We ‘Sf heathy soothe Ne bowel, Pendomanes a i ge significant aroun riveree opined DY ASP a Fnaian com Y sot subjects ge, 1 on : ques and, vitarni Bu eal pest ut hiss unavatiale 8 ‘naan, sal thy southern se ya a east (TOUTS Of TRlebsiella P= 1g anaer0ie, Hn ee erent sc0UNs as srt ey FATE SOUT peas ous organist 2 Gipp rer the mero ec PPC in he the funtion andre ove expecially usefol in cserinalng bet pee wpe wal I dnc iintig ernen . Jamin acy wos ensured DS BEETS rt ie ge os ete Ee en Sea Gehromonst sa the test organist sed Otomo omg wa cae tod Descent Eater an satay 09 TS method St Linnea Tia ga gs st the lowes ate Banas poke fo Nm Sr an press sete Oy ot meal (mesa On ETN bl Reis Newser ati ch) aera TOPS tsar Neu ant Bec a emout i roe ea ee psf oan te, Asutmample gt ee 3 2 Anh tne Ockromanas ea he Hou ny a . Tein By; than that of Euglena the mot es ey tec eT ff Fusobec~ etna comme M pd teenie ra lamas a Toe isioe piace on i WES LO Sapenst , Sc ‘and bioautograpby, ‘of the material prod juce iy and OuoEA au ent at eum ant Me nteroee in he ease OF "Klebsiella the vitae, Bal ea 0 pe wate PO a dh a py ee 0 PT assay five sr ot vitamin Ba setiity Hedomonas te at ines toecn shows Yet aye omen of ‘produce Ho sete a coutebind cosine factor 8 OE an se facts organs, NS Beypat woundNoot Wiamin Bi Roof View sinine — Meepetity, pram Wag ata Wted (ust Feakute) Tated (we insur Mews Neto "Range Preomonss 7S 3 NR wel Rica a 8 2 S34 Cece ye > an "ooe vetinalia Bus 3 46 009 rata ie aoe oo By complex"™”'; alteration of the IF-B,. complex; the Nature Vol. 283 21 February It face HLA att Bd Che AR 190960, FrwisCrfuat ah Ee, 9. Ou 6938-99.9978, A factor(s) in Klebsiella culture filtrates specifically modifies an HLA-B27-associated cell-surface component AF, Geczy, Kerri Alexander & Helen V. Bashir NSW Red Crom Blood Transfusion Service, 153 Clarence Steet, ‘Syeney, NSW, 2000, Australie J. Edmonds [Rheumatolory Department, The St George Hospital, Kogarah, NSW 2217, Aust We have presfously shown"? that a serum raised against certin Isolates of Klebsiella pneumoniae Iyses the Iymphocyies of HLA-27-positive patients with ankylosing sponilitis (AS) but not of B21-positive or 827-negative healthy controls. These ‘observations suggested that some Klebsiella’ ant react with a gene product intimately associated with B27 oF possibly with ‘modified’ B27 (refs 1,3) in patients with AS. It seems likely therefore that some Kicbselfa antizens play arole in the pathogenesis of AS, perhaps by specifically modityine 2 1827 associated cell-surface marker. Wehave now exanined the Influence of euture filtrates rom three Klebsiella Isolates lymphocytes from B27-positive and B2T-negative.heallhy Individuals. We report that 24-h culture filtrates of Klebiela K43 (previously Klebsiella F19) contain a factors) capable of specifically modifying the sensitivity to anti-Rlebsiella K43 serum of B2T"AS lymphocytes (that i cells nat Iysed by anti-Klebsella K43 serum): ‘modilied’ B27" AS lymphocyte: arenow serologically similar tothe cells of B27-positiv paien's ‘with AS (R27°AS*). The falore of untieKlebselis FLD and of anti-Escherichia coli sera tolyse lymphocytes, ws incubated with the corresponding culture filteate>, exch nonspecific lysis by the anti-bacterial sera of target lymmphoeytes bearing bacterial antigens random distributed on tele surtace Therefore, the data ure compatible with a specific t: Formation by a Klebsiella Ka3-derived soluble factor of B27-associated lymphoid cell component. cm | \ | j |